• Journal of Pharmaceutical Investigation
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• v.19 no.4
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• pp.191-194
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• 1989

Bioequivalence test of $Asthcontin^{\circledR}$ tablet, a commercial slow-release theophylline (TP) dosage form, was performed using $Slo-bid^{\circledR}$ capsule as the reference. Since it has been confirmed that the saliva concentration of TP is closely correlated with the plasma concentration in man, the area under the saliva concentration-time curve was used as a bioavailability parameter. The statistical analysis showed that the two dosage forms are equivalent in bioavailability estimating from the saliva concentration. The results supported that the use of soliva as a test sample provides simple and easy techniques for bioequivalence tests of TP-containing dosage forms.

• Journal of Korean society of Dental Hygiene
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• v.17 no.2
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• pp.283-294
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• 2017

Objectives: The purpose of the study was to investigate the effect of calcium concentration in saliva on dental caries activity after consuming calcium. Methods: A total of 59 adult women aged 20 to 40 years were surveyed for calcium intake. The daily average calcium intake was analyzed through dietary records of the subjects. The subjects were divided into two groups based on daily average calcium intake. Salivary pH and concentrations of minerals in the saliva were obtained from A group and B group. Calcium ($Ca^{2+}$) and magnesium ($Mg^{2+}$) concentrations in saliva were measured by HPLC-Ion chromatography using 15 mM sulfuric acid. The dental caries activity test was quantified by salivary buffer capacity test and plaque pH test. Results: The mean $Ca^{2+}$ concentrations of A group was $12.75{\mu}g/m$, the mean $Ca^{2+}$ concentrations in the B group was $16.30{\mu}g/mL$ (p<0.05) and respectively, $Mg^{2+}$ concentrations were found to be $0.48{\mu}g/mL$ and $0.51{\mu}g/mL$. Calcium intake and calcium concentration in saliva showed a significant correlation (r=0.380). Conclusions: The mean $Ca^{2+}$ concentrations in saliva was higher in the high calcium intake group. Therefore, calcium intake in saliva was correlated with dental caries.

• Journal of dental hygiene science
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• v.21 no.3
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• pp.150-157
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• 2021

Background: Stress as a cause of mental health problems is known to be more prevalent in women than in men and has a negative effect on several aspects of physical health, such as the composition of blood and saliva. This study investigated the relationship of perceived stress with blood cell counts, saliva flow rate, and saliva factors. Methods: We recruited women in their 20s with a high prevalence of stress. Stress was evaluated using the Korean version of the perceived stress scale. Blood tests included white blood cell, hemoglobin, and platelet. We then examined the saliva flow rate and cariogenic bacteria level, acidity, occult blood, buffer capacity, leukocyte level, protein level, and ammonia level using rinse water with the SILL-Ha^Ⓡ saliva test system. Results: In a total of 70 participants, the average age was 21.64 years old, the average perceived stress score was 16.96±4.32, and high levels of stress were reported by 80% of the participants (n=56). The high-stress group had lower hemoglobin levels. In addition, the high-stress group showed a lower saliva flow rate than the low-stress group, and there was a difference in the salivary acidity and buffer capacity. The total perceived stress score showed a positive correlation with acidity and negative correlation with buffer capacity and the hemoglobin level. Conclusion: This study found that stress in female college students might affect the composition of blood and saliva. High levels of stress were positively correlated with the hemoglobin level, saliva flow rate, and acidity and negatively correlated with the buffer capacity.

• Journal of Periodontal and Implant Science
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• v.40 no.4
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• pp.164-171
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• 2010

Purpose: This study compared the total antioxidant status (TAS) and superoxide dismutase (SOD) activity in the saliva of periodontally compromised patients before and after scaling and root planing (SRP) to assess their diagnostic utility. Methods: Severe chronic periodontitis patient (test group) and subjects with no attachment loss, sites showing a 3 mm or more probing depth and a sulcus bleeding index < 10% (control group) were enrolled in this study. Saliva sampling and clinical examination were performed at one week, one month and 3 months after SRP. The TAS and SOD activity in each patient's saliva was measured for the comparative analysis between the groups. Results: In the test group, the TAS decreased directly after SRP. With time, it increased slightly and was relatively unchanged compared to the baseline. In the control group, the TAS also decreased immediately after SRP but increased gradually with time until 3 months. The SOD activity in the test and control subjects decreased immediately after SRP until 1 month. At 3 months, the SOD activity had increased. Both groups had a similar profile of SOD activity. However, the SOD activity of the control group was significantly higher than that of the test group at each point in time (P < 0.05). Conclusions: There was a significant difference in the total salivary antioxidant level between the periodontitis and healthy or gingivitis (control) group during the experiment period. The total antioxidant level in the saliva was higher in the patients with severe chronic periodontitis than the healthy or gingivitis control before SRP. The SOD activity of the periodontitis patients was lower than the control at each time point. These findings conclusively reveal the possible use of saliva as a diagnostic tool for periodontal health.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.3
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• pp.298-305
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• 2017

This study aimed to compare chair-side test results for caries risk assessment and evaluate how well the tests reflect caries experience. The study was conducted on children aged < 6 years in primary dentition. Dental examination of children was conducted to determine the dmft index and subjects were divided into two groups : group I (dmft < 6), group II (dmft > 6). This study used four kinds of test kits (Plaque-check PH kit, Saliva-check buffer kit, Saliva-check mutans kit, Cytoperio analysis system). Saliva buffer capacity was significantly low in the high caries experience group (dmft > 6) and correlated with dmft index. Saliva pH level correlated significantly with saliva buffer capacity. The results showed that plaque pH and saliva pH levels had no correlation with dmft index. The Streptococcus mutans level measured by using the Saliva-check mutans and Cytoperio analysis system did not correlate with dmft index.

• Korean Journal of Child Studies
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• v.33 no.3
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• pp.83-97
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• 2012

The purpose of this study is to investigate the effects of sand play therapy on the parenting stress and saliva cortisol levels of parents undergoing child counseling programs. The study was conducted with 13 parents who were undergoing child counseling programs. The sessions were held every week for 45 minutes from July, 2011 to September, 2011. To evaluate the effects of sand play therapy, pre test and post test were conducted and the results were then analyzed. The therapy sessions consist of parents making sand boxes, and this was done without consideration of any particular theme. The instrument used was Abidin (1990)'s Parenting Stress Instrument (PSI). Saliva cortisol levels were obtained at the pre-post stage of the sand play therapy. The data were analyzed by means of frequency and the Wilcoxon Rank Sum Test was conducted by the SPSS. The major findings were as follows; There were significant differences in terms of the decreases in the areas of perception of all the parenting stresses examined, including daily life stress, child temperament stress, child relationship stress, and learning-expectation stress, as well as in saliva cortisol levels. Finally, this study suggests that parenting stress and saliva cortisol levels have a positive relationship, and the effects of sand play therapy were significant with decreases in parenting stress and saliva cortisol levels. There is a clear need for parents undergoing child counseling programs to engage in sand play therapy to decrease parenting stress and saliva cortisol levels.

• Analytical Science and Technology
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• v.21 no.1
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• pp.48-52
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• 2008

A new forensic saliva test method using $SALIgAE^{(R)}$ was evaluated in this study. The sensitivity and specificity of $SALIgAE^{(R)}$ were examined and compared to those of other saliva test methods such as agarose gel diffusion method and $Phadebas^{(R)}$ test sheet method. $SALIgAE^{(R)}$ showed high sensitivity and specificity to human saliva in addition to quickness. Moreover modified $SALIgAE^{(R)}$ method was cheap and easy to use in crime scene and DNA laboratory. $SALIgAE^{(R)}$ was very stable at room temperature and had no effect on STR typing.

• Journal of Korean society of Dental Hygiene
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• v.3 no.2
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• pp.197-208
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• 2003

This study was implemented for 84 students of dental hygiene to show the correlation between dental caries experience and improved caries activity test. Dental caries experience for the sample groups was examined and stimulative saliva secreted for 5 minutes was collected into the tube to check saliva secretion rate. Dentocult LB test was executed to observe Lactobacilli colonies after 96 hour cultivation of culture slides moistened with stimulative saliva. Dentocult SM test(screening strip, site strip) was done to measure SM colonies distribution after 48 hour cultivation of culture strips applied with collected saliva and dental plaque respectively, and salivary buffering capacity was checked by means of Dentobuff strip kit. Following conclusions are obtained after examining the relation between Dentocult LB, Dentocult SM, Dentobuff strip test results and DMFT index, salivary secretion rate. 1. Showed no significant difference between Dentocult LB test results and DMFT index, salivary secretion rate. 2. Showed no significant difference between Dentocult SM(screening strip) test results and DMFT index, salivary secretion rate. 3. Showed significant difference between Dentocult SM(site strip) test results and DMFT index(pE0.05), but showed no significant difference between Dentocult SM(site strip) test results and salivary secretion rate. 4. Showed no significant difference between Dentobuff strip test results and DMFT index, but showed a very wide difference between Dentobuff strip test results and salivary secretion rate(pE0.01).

• Journal of the korean academy of Pediatric Dentistry
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• v.34 no.1
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• pp.73-80
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• 2007

Compomer that release fluoride could be used on proximal caries of child effectively. But oral cavity is always wet, so saliva inhibits bonding of tooth and compomer. When the saliva exist on bonding, it can be occured microleakages. The purpose of this study was to evaluate the influence of salivary contamination on compomer restoration and degree of microleakage according to restoration methods. Dyract $AP^{(R)}$ and prime and $bond^{(R)}$ NT was applied by the manufacture s instructions. Elipar Trilight was applied for light curing. Saliva pool was made for reconstruction of oral cavity. Two premolar was embedded in acrylic resin. After class II cavity preperation, Dyract $AP^{(R)}$ was restored under several condition, the specimen was thermocycled 500 times with 30 second dwell time. 0.5% methylene blue was used for microleakage test. Micoleakage was measured by the ratio of the infiltration length to occlusal and gingival side interface. Data were analyzed statistically using Kruskal Wallis Test, Mann-Whitney Test. The Result were as follows ; 1. In occlusal side, there were no statistical differences. 2. In gingival side, there were no statistical differences in Group III ($ContactMatrix^{TM}$, Rubber dam, $Oraseal^{(R)}$), Group IV (No saliva contamination). 3. In gingival side, there were no statistical differences in Group I$(ContactMatrix^{TM})$, II($ContactMatrix^{TM}$, Rubber dam). 4. In gingival side, there were statistical differences in Group I$(ContactMatrix^{TM})$, II($ContactMatrix^{TM}$, Rubber dam).

• Restorative Dentistry and Endodontics
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• v.25 no.1
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• pp.46-55
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• 2000

During bonding procedure of composite resin, the prepared cavity can be contaminated by saliva. In this study, marginal microleakage and shear bond strength of a composite resin to primed enamel and dentin treated with artificial saliva(Taliva$^{(R)}$) were evaluated. For the marginal microleakage test, Class V cavities were prepared in the buccal surfaces of fifty molars. The samples were randomly assigned into 5 groups with 10 samples in each group. Control group was applied with a bonding system (Scotchbond$^{TM}$ Multi-Purpose plus) according to manufacture's directions without saliva contamination. Experimental groups were divided into 4 groups and contaminated with artificial saliva for 30 seconds after priming: Experimental 1 group ; artificial saliva was dried with compressed air only, Experimental 2 group ; artificial saliva was rinsed and dried. Experimental 3 group ; cavities were etched with 35% phosphoric acid for 15 seconds after rinsing and drying artificial saliva. Experimental 4 group ; cavities were etched with 35% phosphoric acid for 15 seconds and primer was reapplied after rinsing and drying artificial saliva. All the cavities were applied a bonding agent and filled with a composite resin (Z-100$^{TM}$). Specimens were immersed in 0.5% basic fuschin dye for 24 hours and embedded in transparent acrylic resin and sectioned buccolingually with diamond wheel saw. Four sections were obtained from one specimen. Degree of marginal leakage was scored under stereomicroscope and their scores were averaged from four sections. The data were analyzed by Kruscal-Wallis test and Fisher's LSD. For the shear bond strength test, the buccal or occlusal surfaces of one hundred molar teeth were ground to expose enamel(n=50) or dentin(n=50) using diamond wheel saw and its surface was smoothed with Lapping and Polishing Machine(South Bay Technology Co., U.S.A.). Samples were divided into 5 groups. Treatment of saliva-contaminated enamel and dentin surfaces was same as the marginal microleakage test and composite resin was bonded via a gelatin capsule. All specimens were stored in distilled water for 48 hours. The shear bond strengths were measured by universal testing machine (AGS-1000 4D, Shimaduzu Co., Japan) with a crosshead speed of 5 mm/minute. Failure mode of fracture sites was examined under stereomicroscope. The data were analyzed by ANOVA and Tukey's studentized range test. The results of this study were as follows : 1. Enamel marginal microleakage showed no significant difference among groups. 2. Dentinal marginal microleakages of control, experimental 2 and 4 groups were lower than those of experimental 1 and 3 groups (p<0.05). 3. The shear bond strength to enamel was the highest value in control group (20.03${\pm}$4.47MPa) and the lowest value in experimental 1 group (13.28${\pm}$6.52MPa). There were significant differences between experimental 1 group and other groups (p<0.05). 4. The shear bond strength to dentin was higher in control group (17.87${\pm}$4.02MPa) and experimental 4 group (16.38${\pm}$3.23MPa) than in other groups, its value was low in experimental 1 group (3.95${\pm}$2.51 MPa) and experimental 2 group (6.72${\pm}$2.26MPa)(p<0.05). 5. Failure mode of fractured site on the enamel showed mostly adhesive failures in experimental 1 and 3 groups. 6. Failure mode of fractured site on the dentin did not show adhesive failures in control group, but showed mostly adhesive failure in experimental groups. As a summary of above results, if the primed tooth surface was contaminated with artificial saliva, primer should be reapplied after re-etching it.