• Korean Journal of Food Science and Technology
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• v.47 no.3
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• pp.306-320
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• 2015

This survey was carried out in 2014 to estimate the pesticide residue levels in commercial environment-friendly agricultural products in Seoul metropolitan area. Pesticide residues in 27 commodities were examined and analyzed using LC-MS/MS, which can simultaneously detect up to 85 pesticides. A total of 441 samples were collected and analyzed. Among the detected samples, the residue levels in 1.2% of organic agricultural products and 2.2% of pesticide-free agricultural products exceeded the maximum residue limits (MRLs); hence, 1.8% of the total samples exceeded the MRLs. The safety of the detected pesticides was assessed by monitoring the acceptable daily intake level (ADI) and acceptable dietary exposure (ADE) to the pesticides via consumption of the commodities. ADI and ADE are found to be 0.07728-9.46530% and 0.00141-0.17210%, respectively, which means that the residual pesticides in the environmental-friendly agricultural products in Korea are below the safe limit and therefore, pose no potential public health risks.

• Journal of the Korea institute for structural maintenance and inspection
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• v.18 no.3
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• pp.20-27
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• 2014

In the construction site, various earth retaining systems are developed and applied to maintain stability of excavated area and structures. Among the methods, the underground continuous wall and the column-type diaphragm wall methods are especially used in construction site nearby buildings or roads. However, these methods have some disadvantages such as the difficulty of quality control and long curing time because these methods need to cast fresh concrete at the construction site. In addition, these methods are usually applied to the site for the temporary purpose. In this paper, we suggest precast hollow prestressed concrete pile for continuous pile wall system. To investigate the structural behavior of suggested pile, which is the main member of the suggested system, tests pertaining to the structural behavior and prestressing force applied in the pile are conducted. From the test results, it was found that the prestressing force measured is sufficient compared with the value obtained by the design equation and the cracking moment measured is 34% higher than the design value. In addition to the above, this precast hollow prestressed concrete pile has an additional safety margin that the maximum moment is 59.2% higher than the cracking moment which is one of the serviceability limits for the design of the system.

• Nuclear Engineering and Technology
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• v.45 no.5
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• pp.605-612
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• 2013

Removal of decay heat from an operating reactor during a prolonged station blackout condition is a big concern for reactor designers, especially after the recent Fukushima accident. In the case of a prolonged station blackout condition, heat removal is possible only by passive means since no pumps or active systems are available. Keeping this in mind, the AHWR has been designed with many passive safety features. One of them is a passive means of removing decay heat with the help of Isolation Condensers (ICs) which are submerged in a big water pool called the Gravity Driven Water Pool (GDWP). The ICs have many tubes in which the steam, generated by the reactor core due to the decay heat, flows and condenses by rejecting the heat into the water pool. After condensation, the condensate falls back into the steam drum of the reactor. The GDWP tank holds a large amount of water, about 8000 $m^3$, which is located at a higher elevation than the steam drum of the reactor in order to promote natural circulation. Due to the recent Fukushima type accidents, it has been a concern to understand and evaluate the capability of the ICs to remove decay heat for a prolonged period without escalating fuel sheath temperature. In view of this, an analysis has been performed for decay heat removal characteristics over several days of an AHWR by ICs. The computer code RELAP5/MOD3.2 was used for this purpose. Results indicate that the ICs can remove the decay heat for more than 10 days without causing any bulk boiling in the GDWP. After that, decay heat can be removed for more than 40 days by boiling off the pool inventory. The pressure inside the containment does not exceed the design pressure even after 10 days by condensation of steam generated from the GDWP on the walls of containment and on the Passive Containment Cooling System (PCCS) tubes. If venting is carried out after this period, the decay heat can be removed for more than 50 days without exceeding the design limits.

• Food Science of Animal Resources
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• v.34 no.6
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• pp.799-807
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• 2014

Meat source fraud and adulteration scandals have led to consumer demands for accurate meat identification methods. Nucleotide amplification assays have been proposed as an alternative method to protein-based assays for meat identification. In this study, we designed Loop-mediated isothermal amplification (LAMP) assays targeting species-specific mitochondrial DNA to identify and discriminate eight meat species; cattle, pig, horse, goat, sheep, chicken, duck, and turkey. The LAMP primer sets were designed and the target genes were discriminated according to their unique annealing temperature generated by annealing curve analysis. Their unique annealing temperatures were found to be $85.56{\pm}0.07^{\circ}C$ for cattle, $84.96{\pm}0.08^{\circ}C$ for pig, and $85.99{\pm}0.05^{\circ}C$ for horse in the BSE-LAMP set (Bos taurus, Sus scrofa domesticus and Equus caballus); $84.91{\pm}0.11^{\circ}C$ for goat and $83.90{\pm}0.11^{\circ}C$ for sheep in the CO-LAMP set (Capra hircus and Ovis aries); and $86.31{\pm}0.23^{\circ}C$ for chicken, $88.66{\pm}0.12^{\circ}C$ for duck, and $84.49{\pm}0.08^{\circ}C$ for turkey in the GAM-LAMP set (Gallus gallus, Anas platyrhynchos and Meleagris gallopavo). No cross-reactivity was observed in each set. The limits of detection (LODs) of the LAMP assays in raw and cooked meat were determined from $10pg/{\mu}L$ to $100fg/{\mu}L$ levels, and LODs in raw and cooked meat admixtures were determined from 0.01% to 0.0001% levels. The assays were performed within 30 min and showed greater sensitivity than that of the PCR assays. These novel LAMP assays provide a simple, rapid, accurate, and sensitive technology for discrimination of eight meat species.

• The Korean Journal of Pesticide Science
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• v.15 no.4
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• pp.453-462
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• 2011

This study was carried out to survey the residual characteristics of pesticides and assess their safety. Nineteen agricultural commodities, collected from wholesale and traditional markets in Cheongju. Nineteen agricultural commodities including perilla leaves were collected from the markets on October 29th, 2010. Total 240 pesticides which can be analyzed by multiresidue analysis method by GLC and HPLC were monitored and the pesticides detected were confirmed by GC-MSD and LC-MS. Five pesticides, alachlor, bifenthrin, endosulfan, procymidone and triflumizole, were detected from five samples, such as welsh onion, leek and celery in case of wholesale market and perilla leaves and welsh onion in case of traditional market. Detection rate of 13.2% was obtained as a result of pesticide analysis but 2.6% of the pesticides detected exceeded their maximum residue limits. The estimated daily intakes (EDIs) and maximum permissible intakes (MPIs) of the pesticides detected were less than 26% and 0.05% of their acceptable daily intakes (ADIs) respectively, representing that residue levels of the pesticides detected would be safe.

• The Korean Journal of Pesticide Science
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• v.15 no.4
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• pp.441-452
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• 2011

This study was carried out to survey the residual characteristics of endocrine disruptor (ED)-suspected pesticides in greenhouse soils and assess their leachabilites to groundwater. Greenhouse soils were collected from 40 sites of greenhouse in 2008 in Korea. Sixteen ED-suspected pesticides which had been using in Korea, such as alachlor, benomyl, carbaryl, cypermethrin, 2,4-D, dicofol, endosulfan, fenvalerate, malathion, mancozeb, metribuzin, metiram, methomyl, parathion, trifluralin, and vinclozolin, in the soils, were analyzed by chromatographic methods using GLC-ECD and HPLC-DAD/FLD. Limits of detection (LODs) of the test pesticides ranged from 0.0004 to 0.005 mg/kg. Recoveries of the target pesticides from soil ranged from 72.69 to 115.28%. Four pesticides including cypermethrin were detected in the range of from 0.001 to 2.019 mg/kg, representing that their detection rate from greenhouse soils was 37.5%. The highest detection rate was observed from endosulfan which was detected from 16 site soils of the total samples, indicating that endosulfan is persistent in soil because of its very low mobility and high adsorption characteristics in soil. Based on the groundwater ubiquity scores (GUSs) of the pesticides detected from greenhouse soils, most of them have little possibilities of groundwater contamination except the fungicide vinclozolin with some leaching potential because of high water solubility and very low soil adsorption property.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.1
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• pp.1-8
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• 2006

For the effective utilization of tuna processing byproducts such as roes of skipjack tuna (Katsuwonus pelamis) and yellowfin tuna (Thunnus albacores) as a food resource, the roes of skipjack and yellowfin tuna were examined on food component characteristics and also compared to those of Alaska pollack (Theragra chalcogramma). The concentrations of heavy metal in both roe of the skipjack and yellowfin tuna were below the reported safety limits, therefore, these roes appeared to be safe as a raw material for food resource. The contents of crude protein were 21.4% in the skipjack tuna roe and 21.5% in the yellow fin tuna roe, which showed to be the major component in tuna roes. The prominent amino acids of total amino acids were aspartic acid, glutamic acid, leucine and lysine, and these amino acids were comprise to be 38.4-41.2% of total amino acid in both tuna roes. The total lipid content were 2.1 % in the skipjack tuna roe and 2.0% in the yellofin tuna roe. The major component of total lipid was found to be triglyceride in both tuna roes (skipjack tuna roe, 93.3%; yellow fin tuna roe, 92.0%), which was high in the compositions of 16:0, l8:1n-9, and 22:6n-3. The content of DHA in total lipid of the tuna roes (skipjack tuna roe, 29.9%; yellowfin tuna roe, 36.3%) were higher than that of Alaska pollack roe (18.1%). Based on the results of the proximate composition, mineral, amino acid and lipid characteristic, roes of skipjack tuna and yellowfin tuna showed potential as a raw material for food.

• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.321-331
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• 2011

Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture- and biochemical-based assays is time-consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. We developed a novel multiplex real-time PCR assay which was applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081 gene, the multi-copy insertion element in the MTC and the 12.7-kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 10 other bacterial species such as Escherichia coli, and cattle lymph nodes (n=113). The tests identified 96.4% (27/28) as M. bovis from the MTC-positive bTB samples using conventional PCR for specific insertion elements IS1081. And MTC-negative bTB samples (n=85) were tested using conventional PCR and the real-time PCR. When comparative analyses were conducted on all bovine samples, using conventional PCR as the gold standard, the relative accuracy of real-time PCR was 99.1%, the relative specificity was 100%, and the agreement quotient (kappa) was 0.976. The detection limits of the real-time PCR assays for M. bovis and M. tuberculosis genomic DNA were 10 fg and 0.1 pg per PCR reaction, respectively. Consequently, this multiplex real-time PCR assay is a useful diagnotic tool for the identification of MTC and differentiation of M. bovis and M. tuberculosis, as well as the epidemiologic surveillance of animals slaughtered in abattoir.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1467-1474
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• 2006

Fish-frames, which are left after obtaining fillets or muscle during fish processing, consists of useful food components, such as muscle, collagen, calcium, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). This study was carried out to prepare snack using conger eel frame (SF) for human consumption and also to elucidate food component characterization of the snack. The results of volatile basic nitrogen suggested that conger eel frame was a suitable material for preparing snack. Based on the results of sensory evaluation and costs, starch syrup was an optimal sweetener for preparing snack using conger eel frame. The starch syrup-treated SF appeared safe because the moisture content and peroxide value were below the safety limits described in the guideline of Korea Food and Drug Administration (KFDA). Starch syrup-treated SF was similar in the pattern of fatty acid composition to soybean oil, whereas EPA and DHA were detected in SF. The total content of amino acid in starch syrup-treated SF was 23.9% based on 100 g of raw material. The maj or amino acids were aspartic acid, glutamic acid, glycine and alanine. The total contents of calcium and phosphorus in starch syrup-treated SF were 4.9% and 2.8%, respectively. The Ca/P of starch syrup-treated SF was 1.9, which is a good ratio for absorption of calcium. The SF made with starch syrup was superior in EPA and DHA compositions, total amino acid, calcium and phosphorus contents to commercial snack using eel frame.

• Korean Chemical Engineering Research
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• v.44 no.2
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• pp.216-226
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• 2006

Total trihalomethanes (THMs), dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA) that are the major disinfection byproducts (DBPs) are monitored continuously in drinking water in Seoul. Study on characteristics of DBPs is crucial to judge the safety of drinking water in Seoul. Analysis of THMs, haloacetonitriles (HANs), chloral hydrate (CH), and haloacetic acids (HAAs) was carried out in several distribution systems from January 2002 to December 2004. The concentration of THMs was 0.015 mg/L in purified water, 0.019 mg/L in tapwater by direct service, and 0.023 mg/L in tapwater through watertank, respectively. It might be due to the increased contact time with chlorine by a process of the distribution system. And the other DBPs show a tendency to increase in its concentration by a process of the distribution system. Also, in summer, the concentration of DBPs was higher than in spring and winter. It might be due to the higher temperature of water in summer. In all cases, the quantities of detected DBPs were 4-6 times lower than those of regulation limits of drinking water in Seoul. In view of these results, the tapwater in Seoul is good to drink it all the times.