• Journal of Food Hygiene and Safety
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• v.8 no.4
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• pp.205-214
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• 1993

The monoclonal antibody to chloramphenicol(CAP) was produced to develop an enzyme-linked immunosorbent assay(ELISA) for residual CAP. An immunogen(CAP-BSA) was prepared by immunogen, antibody titer was measured by indirect ELISA. Spleen cells form the immunized mouse were fused with SP2/OAg14 myeloma cells. Among hybridomas selected in HAT media, 6 clones shown high antibody titer to CAP were subjected to cloning by limit dilution, and all of the monoclonal antibodies(MCA1, 2, 3, 4, 5, 7 and 9) produced by each clone were identified as IgG1 by ELISA isotyping analysis. Competitive ELISA condition was established by using the purified monoclonal antibody MCA1 as primary antibody and CAP-HSA conjugate as coating antigen. Standard curve of CAP(n=28) showed that the lowest detection limit of CAP is 20ng/ml level. The cross-reactivities of the 6 monoclonal antibodies showed that CAP sodium succinate. CAP base, P-nitrophenol, and p-nitrobenzyl alcohol were 89∼178, 0.050∼2.237, 0.056∼0.794 and 0.013∼7.939%, respectively. No cross-reactivities were observed with phenylalanine, tyrosine, glutamine, thiamphenicol, neomycin, streptomycin, gentamicin, sulfamethazine, sulfathiazole, chlortetracycline and p-aminobenzoic acid.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05b
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• pp.657-662
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• 1996

Venting capability to prevent excess pressurization caused by loss of Residual Heat Removal System (RHRS) during mid-loop operation hat been evaluated analytically and the peak Reactor Coolant System (RCS) pressure was compared with the results of the MIDLOOP computer code. Even though analytical method if relatively simple, the results are in a good agreement with those of the computer code. For both methods, the peak pressures have not, exceeded the nozzle dam design pressure, if the vent paths such as pressurizer safety valves or a pressurizer manway are available in a closed RCS configuration with the nozzle dam installed.

• Proceedings of the KWS Conference
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• 2006.10a
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• pp.45-47
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• 2006

Lightweight design, safety, vibration, energy absorption capability can no longer achieve without adhesive bond joint. Base on those concepts and cost reduction, adhesive bond FEM model development has been done. The FEM analysis and experiments were conducted in this study. Crash condition is 143 Kg Hammer weight and unit meter Height. and then These test results were used to develop resonable FEM model. To estimate which FEM model is resonable, compare hybrid joint specimen experiment results with FEM analysis results. Conclusively this study achieves optimization bonding model related crash and adhesive bond jointed hat profile crash characters.

• Journal of Mechanical Science and Technology
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• v.16 no.2
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• pp.262-269
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• 2002

The effects of velocity boundary conditions on the structure of methane-air nonpremixed counterflow flames were investigated by two-dimensional numerical simulation. Two low global strain rates, 12 s$\^$-1/ and 20 s$\^$-1/, were considered for comparison with measurements. Buoyancy was conformed to have strong effects on the flame structure at a low global strain rate. It was shown that the location where a top hat velocity profile was imposed is sensitive to the flame structure, and that the computed temperature along the centerline agrees well with the measurements when plug flow was imposed at the inner surface of the screen nearest the duct exit.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.2
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• pp.92-99
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• 2006

Design capability for high safety vehicle with light weight is crucial to enhancing competitive power in vehicle market. The structural foam can contribute to restraining section distortion in body members undergoing bending collapse at vehicle crash. In this study, first, the validation of analysis model including structural foam model for simulating fracture behavior was discussed, and the bending collapse characteristics of five representative section types were analyzed and compared. Next, with changing the laminate foam shape, load carrying capability and absorbed energy were observed. The results suggests a design strategy of body members filled with laminate foam, leading to effectively elevating bending collapse characteristics with weight increase in the minimum.

• Journal of Korea Ship Safrty Technology Authority
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• s.23
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• pp.51-64
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• 2007

Main concern of this subject is "hat type of hull shape is most important for building aluminum fishing vessel and suitable for domestic fisherman." For the this subject, We research from traditional hull shape of fiberglass fishing vessel and supply to our domestic fisherman with 3D render images. Following the above job making with concept design and basic drawing for product model. And most important research job with technical of aluminum vessel and products system and standard of job description. We filled two category with design and products for our domestic fisherman.

• Steel and Composite Structures
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• v.36 no.4
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• pp.383-398
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• 2020

This paper investigates the progressive collapse behavior of 3D steel-framed gravity buildings under fires with a cooling phase. The effect of fire protections and bracing systems on whether, how, and when a gravity building collapses is studied. It is found that whether a building collapses or not depends on the duration of the heating phase, and it may withstand a "short-hot" fire, but collapses under a mild fire or a "long-cool" fire. The collapse time can be conservatively determined by the time when the temperature of steel columns reaches a critical temperature of 550 ℃. It is also found that the application of a higher level of fire protection may prevent the collapse of a building, but may also lead to its collapse in the cooling phase due to the delayed temperature increment in the heated members. The tensile membrane action in a heated slab can be resisted by a tensile ring around its perimeter or by tensile yielding lines extended to the edge of the frame. It is recommended for practical design that hat bracing systems should be arranged on the whole top floor, and a combination of perimeter and internal vertical bracing systems be used to mitigate the fire-induced collapse of gravity buildings. It is also suggested that beam-to-column connections should be designed to resist high tensile forces (up to yielding force) during the cooling phase of a fire.

• Journal of the FoodService Safety
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• v.2 no.2
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• pp.97-102
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• 2021

The purpose of this study was to investigate the effect of developing and continuously providing on-line hygiene education programs on the improvement of hygiene conditions in children's cafeterias. As a result of the 2020 sanitation and safety checklist analysis, 6 items (personal hygiene, separate use, clean ventilation, temperature control of refrigerators, country of origin, food distribution) were derived and on-line hygiene education programs for each of 6 items were produced. ① Customized educational materials and self-inspection checklists were provided to 208 children's cafeterias. After that, educational videos were provided through Kakao Talk twice a week for 6 months, and they were made available for viewing at any time through YouTube upload, ③ Kakao Talk Through this, a quiz related to the educational video was conducted to give feedback for interaction with the cook. As a result of analyzing the total hygiene and safety checklist score of all registered facility catering centers by visit order, in 2020 it was 82.8 points/100 points, but in 2021, it was 84.2 points (1st round), 89.3 points (2nd round), 91.4 points (3 points) The score improved significantly (p<0.001) as the on-line hygiene education program continued. As a result, significant (p<0.001) changes were observed in the items of 'Knife, chopping board' and 'Sanitation clothes, sanitary hat, sanitary shoes, apron, and sanitary gloves', confirming a clear improvement effect. Therefore, it is considered that the on-line hygiene education program will play a positive role in showing a lasting effect on improving hygiene management in children's cafeterias.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.23 no.3
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• pp.243-249
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• 2014

Currently, CFRP composites are rapidly replacing steel plates, as they are lighter, stronger, and more elastic; however, they are poorly suited to hygrothermal and impact-collapsed environments because moisture can alter their molecule arrangement and chemical properties. In this study, environments are experimentally simulated in order to investigate changes in the moisture absorption inside a CFRP composite and to determine its weakest point. Moreover, changes in the moisture absorption ratio at temperatures of $60^{\circ}C$ and $80^{\circ}C$ are studied and compared in order to understand how changes in temperature affect moisture absorption inside CFRP composites. Results show that moisture absorption leads to a strength reduction of around 50%. In addition, the moisture absorption rate inside CFRP composites is shown to change rapidly with increasing temperature. Accordingly, it showed that the change in matrix also has a weak point.

• Journal of the Korean Society of Clothing and Textiles
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• v.30 no.4 s.152
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• pp.542-553
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• 2006

The purpose of this study were to analyse of craniofacial shape by 3D and to develope of head models for Korean adults with gender and age groups. The 3D measurement technique adapted in this study was a novel approach compared that the same technique has been commonly used in measuring human bodies. The data and the model of head analysis can be used as a basic reference in developing various head related items such as hat, helmet, gas mask, ear phone, and etc. In this study, heads of 836 Korean adults were measured in 3D, analyzed by statistical methods, and modelized in 3D by gender and age groups. From the basic statistical data analysis, vertex-tragion and the length between the pupils were the longest in their twenties for both men and women, and grew shorter in elderly groups. In all categories, a significant difference appeared between men and women in their 20's, but the differences were less noticeable in elderly groups. Compared to the one size standard head model of the Korea Occupational Safety and Health Agency, the above three-dimensional standard head model would provide a more through fit because gender and age groups were sub-divided and analyzed in 3D.