• Mycobiology
• /
• 제38권2호
• /
• pp.144-148
• /
• 2010

$\beta$-Glucans have been known to exhibit antitumor activities by potentiating host immunity by an unknown mechanism. The C-type lectin dectin-1, a $\beta$-glucan receptor, is found on the macrophage and can recognize various $\beta$-glucans. Previously, we demonstrated the presence of $\beta$-glucan receptor, dectin-1, on the Raw 264.7 cells as well as on murine mucosal organs, such as the thymus, the lung, and the spleen. In order to investigate immunopotentiation of innate immunity by $\beta$-glucan, we stimulated a murine macrophage Raw 264.7 cell line with $\beta$-glucans from Pleurotus ostreatus, Saccharomyces cerevisiae, and Laminaria digitata. Then, we analyzed cytokines such as tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6 by reverse transcription-polymerase chain reaction (RT-PCR). In addition we analyzed gene expression patterns in $\beta$-glucan-treated Raw 264.7 cells by applying total mRNA to cDNA microarray to investigate the expression of 7,000 known genes. When stimulated with $\beta$-glucans, the macrophage cells increased TNF-$\alpha$ expression. When co-stimulation of the cells with $\beta$-glucan and lipopolysaccharide (LPS), a synergy effect was observed by increased TNF-$\alpha$ expression. In IL-6 expression, any of the $\beta$-glucans tested could not induce IL-6 expression by itself. However, when co-stimulation occurred with $\beta$-glucan and LPS, the cells showed strong synergistic effects by increased IL-6 expression. Chip analysis showed that $\beta$-glucan of P. ostreatus increased gene expressions of immunomodulating gene families such as kinases, lectin associated genes and TNF-related genes in the macrophage cell line. Induction of TNF receptor expression by FACS analysis was synergized only when co-stimulated with $\beta$-glucan and LPS, not with $\beta$-glucan alone. From these data, $\beta$-glucan increased expressions of immunomodulating genes and showed synergistic effect with LPS.

• 정보화정책
• /
• 제25권2호
• /
• pp.3-19
• /
• 2018

경찰이 범죄예방과 수사를 위해 영상장비를 활용하고 있는 것은 비단 최근의 일만은 아니다. 과거 캠코더나 열화상 카메라와 같은 새로운 영상장비들이 개발되어 사용되면서부터 꾸준히 범죄에 대응하기 위한 수단으로 사용되어 왔다. 하지만 최근 들어 지능형 CCTV나 드론의 영상장비, 개량된 열화상 카메라 등과 같이 관련 기술들이 더욱 진화하고, 해당 영상 장비들을 범죄대응 목적으로 사용할 수 있는 근거 법령들이 뒷받침 되면서 그 활용범위가 보다 넓어지고 있다. 그에 따라 범죄예방 목적 뿐 아니라 범죄수사, 형사소송절차에도 널리 사용되고 있다. 그러나 영상장비의 활용이 많아짐에 따라 다양한 문제점들도 제기되고 있다. 최근 범죄대응 영상장비의 활용과 관련된 문헌들을 살펴보면 근거규정 미비, 개인정보 침해 위험성, 사생활 자유침해, 그리고 영상장비의 보안침해에 대해 꾸준히 문제점들을 제시하고 있다. 앞으로 영상장비는 보다 다양한 모습으로 범죄대응을 위해서 사용될 것이고, 그 기술 또한 더욱 발전할 것이다. 하지만 지금 시점에서 제기되고 있는 문제점을 해결하지 않는다면 앞으로 더욱 큰 문제들이 발생할 수 있다. 따라서 입법자들과 정부는 제기되는 문제점들을 면밀히 검토하여 영상장비들이 시민의 인권을 침해하지 않으면서 시민의 안전을 위해 활용될 수 있도록 노력해야 할 것이다.

• 한국철도학회논문집
• /
• 제17권6호
• /
• pp.423-432
• /
• 2014

소비자의 구매행동이 기업에 의해 제공되는 서비스 품질뿐 아니라 기업의 사회적 책임에도 영향을 받는다고 보고되며, 사회적 책임 활동은 효율적, 전략적 업무 수행에 필요한 기업의 핵심 요인으로 인식되고 있다. 본 연구는 지금까지 사기업을 중심으로 연구된 사회적 책임 요인을 공기업인 철도공사에 적용함으로써 철도이용자의 행동에 어떤 영향을 미치는지 파악하기 위해 이루어졌다. 철도이용자를 대상으로 설문조사를 실시한 결과, 철도공사의 경제적, 법적 책임 활동이 철도공사의 신뢰에 유의한 영향을 미치며, 신뢰는 철도공사에 대한 고객 충성도에 유의한 긍정적 효과를 미치는 것을 검증했다. 본 연구를 통해 철도공사는 법적 책임 활동과 경제적 책임 활동을 수행함으로써 고객 신뢰를 확보해야 함을 확인 할 수 있었으며, 또한 철도이용자들에게 고객 충성도를 유발시키기 위해 전략으로 준법경영프로그램의 도입, 열차안전관리 정보 제공, 지역 산업과의 연계를 통한 맞춤형 관광 상품의 개발, 커뮤니케이션 전략을 이행할 것을 제안하였다.

• 융합정보논문지
• /
• 제7권6호
• /
• pp.89-95
• /
• 2017

암을 유발하는 PMA는 세포를 자극하여 전사인자 C-Jun/C-fos의 발현을 증가시키며 핵 내의 AP-1의 활성을 증가하게 되고 결과적으로 각종 암이 발생된다고 보고되고 있다. 본 연구에서는 천연물 지표성분의 안전성을 보기위한 세포독성과 암예방 효과를 예측할 수 있는 Activator protein(AP-1) 활성억제를 관찰하였다. 천연물 지표성분 38종을 대상으로 세포독성과 AP-1 활성억제를 관찰한 결과 섬수의 지표성분인 Bufogein 과 Cinobufagin에서는 세포독성을 보이며 AP-1 활성 억제와 비교 시 5배 정도 차이를 보임을 알 수가 있다. 반면에 Arctigenin, Manassantin A, B에서는 AP-1 활성 억제 농도는 $2{\mu}M$ 이하이면서 세포독성과의 비율은 15배 이상임을 알 수 있다. 본 실험의 결과를 통해 우방자와 삼백초의 지표성분인 Arctigenin, Manassantin A,B는 암 예방 연구의 가능성을 시사하였다.

• 한국주거학회:학술대회논문집
• /
• 한국주거학회 2002년도 추계학술발표대회
• /
• pp.137-142
• /
• 2002

The purpose of this study was to examine the features that make residents feel “at home” in ALFs in Southwest Virginia and to suggest further policy and design guidelines for better Quality of ALFs as a “home.” For this purpose, residents' needs, experiences, and opinions of the physical environment, the social environment, and the organizational environments such as policies and programs of ALFs were identified. As a multi-case study, five ALFs in Southwest Virginia were studied using constant comparative methos of data analysis. In addition to face-to-face interviews with 25 residents and five administrators of five ALFs, observations were conducted with personal journal. Overall, the five sites selected presented homelike features showing the philosophy of assisted living which combines housing and services. Each facility was designed to be a single-family house or multi-family dwelling in outside appearance. As a whole, residents felt isolation and loneliness and they did not have active interaction with other residents because of diverse background among the residents. However, all of them had close relationships with the staff. The staff's attitude and behavior seemed to influence greatly the residents' feeling “at home.” Despite the provision of diverse activities by the facilities, many residents did not participate in the programs. Most of the residents agreed that the rule and regulations were fair. In spite of high satisfaction with the facility, many people did not think of their current dwelling as a real ‘home.’ As the biggest difference between living in their own homes and living in the ALF, people pointed out a lack of independence, freedom, and autonomy. Residents of ALFs may have reordered their priorities in their current life situation so that safety, security, and care were more important to them than feeling “at home.” Among the three factors --physical, social, and organizational-- that affect the residents' perception of ALFs as a “home, ” many emphasized the importance of social factors such as relationships with the staff and residents, and social support from their family or friends.

• 한국임상약학회지
• /
• 제8권1호
• /
• pp.1-12
• /
• 1998

Rheumatoid arthritis (RA) is a common systemic inflammatory disease which DMARDS have been widely used as a treatment modality both as monotherapy and combination therapy Bucillamine, one of newer DMARDS, has recently proven its efficacy as monotherapy in the treatment of RA. The objective of this study was to compare the efficacy and the safety of bucillamine monotherapy and bucillamine plus methotrexate combination therapy in the treatment of rheumatoid arthritis. Forty-nine mild RA patients were enrolled in this prospective, open-trial and were assigned to receive bucillamine 200 mg/day (n=18) or bucillamine 200 mg/day and methotrexate 7.5-15 mg/week (n=31) orally for 16 weeks. Concomitant use of NSAID and prednisolone <5 mg/day or equivalent dose of steroid were allowed. Both monotherapy group and combination therapy group have shown significant improvement in disease activities (Ritchie index, painful joints, swollen joints, morning stiffness, grip strength, ESR, RF, CRP, patient's self assessment of pain, physician's global assessment of disease activity) from the baseline. However, there was no statistically significant difference between two groups. The adverse effects were more frequently shown in combination therapy group than monotherapy group. In conclusion, in patients with mild RA monotherapy has shown to be equally efficacious as combination therapy with less side effects.

• 지역사회간호학회지
• /
• 제14권3호
• /
• pp.488-495
• /
• 2003

Purpose: This study was attempted to provide the basic data in order to develop a supportive system for the family by investigation of the general and disability-related features of the industrial accident disabled and their family function. Method: Three hundred and forty six industrial accident disabled were randomly selected among those who resided in the Seoul, Incheon, and Gyeonggi areas, and who were confirmed as disabled according to its severity from the first to fourteenth grade from January, 1998 to December, 2000. The data were collected for one month from August 1 to August 31, 2001. Each study team consisted of two members out of 20 researchers and they interviewed the disabled individually by visiting their homes. Collected data were analyzed using frequency, percentages, t-test, ANOVA, and Correlation, etc. Results: The average score for family function was 102.60. The adaptability represented the highest score among the concepts dependent on the family function. The concepts were cohesion, adaptability and communication, which scored as 33.59, 35.38, 33.34, respectively. The family function was significantly different in education, marital status and subjective health status. In particular, the family function was excellent from the industrial accident disabled who had graduated from middle school, was married, and felt healthy. Depression and quality of life were other factors, which were also correlated significantly with the family function. The lower the degree of depression and the better the quality of life, the better the family function. Conclusion: The industrial accident disabled who had been in poor health or faced with difficulties in daily activities even after the therapy for the disability was completed. In particular, their family always had a great burden to care for the disabled and to take financial responsibility. Therefore, the rehabilitation program for the disabled should be family oriented.

• 한국패션뷰티학회지
• /
• 제5권4호
• /
• pp.130-138
• /
• 2007

The aim of this study was to assess the cosmeceutical activies of four kinds of Korea herb medicine extracts using in cosmetics and related industries. The cosmeceutical activities of extracts were investigated by tyrosinase inhibition, astringent, anti-bacterial and MTT assay for cell viability. In the whitening effect, PA that the highest tyrosinase inhibition activity showed 56% at 10 ppm in ethanol extract. Also water and ethanol extract of RE showed 54%, 68% at 1,000 ppm, respectively, but LE and CA showed lower effect. Astringent effect of water and ethanol extract of PA appeared over 60% at 1,000ppm concentration but other extracts showed no astringent effect. In the anti-bacterial test, water and ethanol extract of PA showed no anti-bacterial effect against all microorganisms. But water and ethanol extract of RE showed anti-bacterial effect on Staphylococcus aureus, ethanol extract of showed on Staphylococcus aureus and Propionibacterium acnes, ethanol extract of CA showed on Candida albicans. The resUlt of stability test showed that the emulsion of containing PA were very stable at various temperature and sun-light test. Viscosity and pH of emulsion did not change. From the results of human patch test to assess the safety of cosmetics containing PA there was no negative reaction on skin was found.

• Preventive Nutrition and Food Science
• /
• 제9권4호
• /
• pp.361-366
• /
• 2004

Defatted methanol extracts of the medicinal plants, Rubus coreanus Miq. (RC) and Atractylodes japonica Koidzumi (AJ) were added at the levels of 0.1, 0.5, or $2\%$ (w/w) to high cholesterol diets and fed to ovariectomized Sprague-Dawley female rats, weighing 212.6 $\pm$ 1.8 g for four weeks. Weight gains were lower in RC and AJ groups than the control group, but there were no changes in uterus weights. Serum levels of triglyceride decreased by 20-$27\%$ in the experimental groups fed $0.1\%$ of each extract (O.1RC and O.1AJ), compared with that of control (Ovx). Serum cholesterol levels were not changed in the RC groups but increased in the group fed $2\%$ of the AJ extract. Liver levels of cholesterol and triglyceride were reduced in both the RC and AJ groups. Microscopic observation revealed that there were no morphological alterations in liver, lung, heart, spleen and kidney tissues of the experimental groups. Plasma levels of albumin, BUN, creatinine, sodium, potassium and phosphate in the IRC and AJ groups were in normal ranges. Serum GOT and GPT activities were, however, higher in the 2.0AJ than Ovx group. These results suggest that the extracts of the Rubus coreanus Miq. and Atractylodes japonica Koidzumi at dietary levels as low as $0.1\%$ may be utilized as hypotriglyceridemic ingredients for functional foods.

• 한국식품영양과학회지
• /
• 제45권11호
• /
• pp.1696-1700
• /
• 2016

본 연구에서는 차아염소산 나트륨 같은 염소계 살균세척제를 대체하기 위한 천연 살균세척제로서 도토리박 추출물(APE)을 개발하기 위해 적근대에 Listeria monocytogenes를 접종한 후 APE의 항균 효과를 측정하였다. 그리고 최적 세척처리 조건을 수립하고자 APE, FA, $50^{\circ}C$에서의 mild heat를 병합처리하였다. 모든 처리구 중 0.5% APE/$50^{\circ}C$ mild heat/0.5% fumaric acid(FA) 병합 세척처리가 가장 효과적이었는데, L. monocytogenes 수를 대조구에 비해 3.36 log CFU/g 감소시켰다. 병합처리된 적근대 시료의 저장 8일 후 총 호기성균은 대조구와 비교하였을 때 2.89 log CFU/g 감소하였고, 색도 측정 결과 처리구 간 유의적인 차이가 나타나지 않았다. 따라서 본 연구 결과 APE/mild heat/FA 병합처리 방법은 수확 후 적근대의 품질에 영향을 미치지 않고 미생물학적 안전성을 높일 수 있는 효과적인 기술이라고 판단된다.