• Title/Summary/Keyword: rub test

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Mediation of Rubradirin Resistance by ABC Transporters (RubT1) from Streptomyces achromogenes var. rubradiris NRRL3061

  • Lamichhane, Janardan;Oh, Tae-Jin;Lee, Hei-Chan;Liou, Kwang-Kyoung;Kim, Chun-Gyu;Sohng, Jae-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.16 no.12
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    • pp.1928-1934
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    • 2006
  • The rubradirin biosynthetic gene cluster harbors 58 ORFs within a 105.6-kb sequence, which includes all of the genes responsible for the synthesis of rubradirin, as well as the primary genes relevant to regulatory, resistance, and transport functions. This gene cluster also harbors a resistance-mediating ABC transporter, RubT1, which is located at the most upstream position in the cluster. In the present study, RubT1 was expressed heterologously in E. coli, and the resistance affinity of RubT1 was determined by an antibacterial activity test, as well as by HPLC and ESI-MS analyses. Evidence clearly demonstrates that RubTl mediates rubradirin resistance as an ABC transporter.

A Study on Copper hydroxide affecting the Curing and the Corrosion resistance of Electrocoating (수산화구리가 전착도막의 경화성과 내식성에 미치는 영향)

  • Yang, Wonseog;Hwang, Woonsuk
    • Corrosion Science and Technology
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    • v.12 no.5
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    • pp.253-258
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    • 2013
  • Effects of copper hydroxide(II) on the curing and the corrosion resistance of electrocoating were investigated by MEK rubbing test, electrochemical impedance spectroscopy (EIS) and Thermo-gravimetric analysis (TGA). Curing performance of electrocoating was lowered with increasing the content of copper hydroxide(II) as evidenced by the MEK rub performance which decreased with increasing the content of copper hydroxide(II). This indicates copper hydroxide(II) affected the blocked isocyanate reaction in the coatings, by the decomposition of copper hydroxide(II) to CuO and $H_2O$ during reaction of isocyanate with nuclephiles. Corrosion resistance of coatings also decreased with the content of copper hydroxide. This reflects the higher barrier property in coatings with higher curing performance.

A Study on the Manufacturing of Cellular RubBer Products with NR, SBR, NBR EPDM EPDM (NR, SBR, NBR 및 EPDM 발포(發泡)고무의 제조연구(製造硏究))

  • Choi, Sei-Young;Paik, Nam-Chul
    • Elastomers and Composites
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    • v.16 no.1
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    • pp.3-13
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    • 1981
  • The purpose of this dissertation is to study the physical properties of cellular rubber products for industrial use. Vulcanization characteristics were investigated by usingcure curve that had obtained by means of Rheometer. The results of physical properties, vulcanization characteristics and foaming states are as follows. 1. The test results for vulcanization characteristics of NR compounds indicated that in the recipe R-1. When accelerator D is used, the optimum conditions of vulcanizate are obtained, while formula R-2 and R-3 have shown higher torgue at curing time, $1{\sim}2$ minutes. Cellular rubber product test in terms of compression set and compression deflection has also met the requirements of SAE. 2. For SBR compounds, S-1 formula was the best in terns of vulcanization characteristics, and for the blowing structure of cellular rubber products, formula S-3 in which accelerator M is added was fair. All other test results, such as compression set and compression deflection properties met SAE requirements. 3. NBR compound (N-1) including accelerator TT was the best in terms of vulcanization characteristic and also blowing structure. All other properties listed above met requirements, particulary for oil resistance test. 4. In the test of EPDM compounds, when mixed accelerator, M and TT, is used(formula E-1) the best results were obtained. Since EPDM is hydrocarbon elastomer, oil resistance test failed. All other properties met the requirement specified in SAE.

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A Comparison of Antimicrobial Effect of Two Waterless Alcohol-based Hand Rubs with a Povidone-Iodine Hand Scrub for Surgical Hand Antisepsis (두 가지 알코올제제 손마찰과 포비돈 아이오다인의 외과적 손소독 효과 비교)

  • Ju, Houng Ley;Jeong, Jae Sim;Kim, Mi Na;Park, Kwang Ok
    • Journal of Korean Clinical Nursing Research
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    • v.15 no.1
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    • pp.55-65
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    • 2009
  • Purpose: The purpose of this study was to compare the 1% chlorhexidine gluconate/61% ethanol (CHG/ethanol), 45% ethanol/18% 1-propanol (ethanol/propanol) and 7.5% povidone-iodine (PVI) scrub with brush to evaluate their antimicrobial effect. Method: Utilizing repeated measures design, 9 nurses participated in the study. Glove juice sampling procedure was used to evaluate microbial hand counts before the surgical hand antisepsis, one minute after hand wash, and after the surgery. Results: Waterless rub using CHG and ethanol combination resulted in a 3.94 log reduction at 1 min and 2.78 log reduction at 3 hrs. Ethanol/propanol resulted in a 2.42 at 1 min and 2.22 at 3 hrs. The traditional scrub using PVI with brush resulted in a 0.94 at 1 min and 0.95 at 3 hrs (p=.003) and 3 hrs (p=.026) after the surgical hand antisepsis. Repeated measures ANOVA results showed that there was a statistically significant difference among group (p=.002). Duncan post hoc test result showed that the PVI was less effective (p<.05) in sterilizing microbials on hands than CHG/ethanol or ethanol/propanol. Conclusion: Both of the two alcohol-based antiseptic rubs are acceptable alternatives to the PVI with brush for surgical hand antisepsis.

Antioxidant Activities and Quality Characteristics of Gravy Sauce Added with Bokbunja (Rub coreanus Miquel), Mulberry and Blueberry (복분자, 오디 및 블루베리 첨가 그레이비소스의 품질특성 및 항산화 활성)

  • Ra, Ha Na;Kim, Nam-Geun
    • Korean journal of food and cookery science
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    • v.32 no.4
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    • pp.458-465
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    • 2016
  • Purpose: Bokbunja (Rub coreanus Miquel), mulberry and blueberry have been reported to have powerful anti-antioxidant and anti-aging activities. The purpose of this study was to compare the quality characteristics and antioxidant activities of gravy sauce added with Bokbunja, mulberry and blueberry. Methods: Quality characteristics pH, brix, color and sensory characteristics of samples were evaluated. Antioxidant activities of samples were compared using total phenol, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. Results: Mulberry juice showed significantly (p<0.05) higher values of pH and $^{\circ}Bx$ compared to Bokbunja and blueberry juice. Bokbunja juice also had significantly (p<0.05) higher value of Color-L value than mulberry and blueberry juice. The total phenol contents and ABTS cation radical scavenging activity of Bokbunja juice were 236.50 mgGAE/100 g and 61.66%, respectively, which were significantly (p<0.05) higher than those of blueberry and mulberry juice. pH value of gravy sauce added with mulberry (MBS) was 4.67, which was significantly higher than that of the control gravy sauce (GS) and gravy sauce added with Bokbunja (BJS) which had the lowest (p<0.05) pH value 4.33. $^{\circ}Bx$ of GS (13.50) was not significantly different from that of MBS and BBS. However, BJS showed the lowest (p<0.05) value $12.33^{\circ}Bx$. Total phenol contents and ABTS cation radical scavenging activity of BJS were 78.09 mgGAE/100 g and 14.47%, respectively, which were significantly (p<0.05) higher than those of MBS or BBS. Values of sensory characteristics color, sour aroma and sour flavor of BJS were significantly (p<0.05) higher value than those of other samples. Conclusion: Bokbunja juice and gravy sauce showed higher antioxidant activities and higher acceptance test compared to blueberry and mulberry. Thus, Bokbunja has the potential to be added to as high value products in the food industry.

Stress Corrosion Cracking Behavior of Alloy 690 in Crevice Environment (Pb + S + Cl) in a Steam Generator Tube (증기발생기 전열관 틈새복합환경(Pb+S+Cl)에서 Alloy 690의 응력부식균열거동)

  • Shin, Jung-Ho;Lim, Sang-Yeop;Kim, Dong-Jin
    • Corrosion Science and Technology
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    • v.17 no.3
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    • pp.116-122
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    • 2018
  • The secondary coolant of a nuclear power plant has small amounts of various impurities (S, Pb, and Cl, etc.) introduced during the initial construction, maintenance, and normal operation. While the concentration of impurities in the feed water is very low, the flow of the cooling water is restricted, so impurities can accumulate on the Top of Tubesheet (TTS). This environment is chemically very complicated and has a very wide range of pH from acidic to alkaline. In this study, the characteristics of the oxide and the mechanism of stress corrosion cracking (SCC) are investigated for Alloy 690 TT in alkaline solution containing Pb, Cl, and S. Reverse U-bend (RUB) specimens were used to evaluate the SCC resistance. The test solution comprises 3m NaCl + 500ppm Pb + 0.31m $Na_2SO_4$ + 0.45m NaOH. Experimental results show that Alloy 690 TT of the crevice environment containing Pb, S, and Cl has significant cracks, indicating that Alloy 690 is vulnerable to stress corrosion cracking under this environment.

Study on Rub Vibration of Rotary Machine for Turbine Blade Diagnosis (터빈 블레이드 진단을 위한 회전기계 마찰 진동에 관한 연구)

  • Yu, Hyeon Tak;Ahn, Byung Hyun;Lee, Jong Myeong;Ha, Jeong Min;Choi, Byeong Keun
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.26 no.6_spc
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    • pp.714-720
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    • 2016
  • Rubbing and misalignment are the most usual faults that occurs in rotating machinery and with them severe effect on power plant availability. Especially blade rubbing is hard to detect on FFT spectrum using the vibration signal. In this paper, the possibility of feature analysis of vibration signal is confirmed under blade rubbing and misalignment condition. And the lab-scale rotor test device provides the blade rubbing and shaft misalignment modes. Feature selection based on GA (genetic algorithm) is processed by the extracted feature of the time domain. Then, classification of the features is analyzed by using SVM (support vector machine) which is one of the machine learning algorithm. The results of features selection based on GA compared with those based on PCA (principal component analysis). According to the results, the possibility of feature analysis is confirmed. Therefore, blade rubbing and shaft misalignment can be diagnosed by feature of vibration signal.

Effects of Ground Exercise for Arthritis Program in Person with Chronic Arthritis (만성관절염 환자에 대한 관절염체조의 효과)

  • Sohang, Kyeong-Yea;Kang, Sung-Sil
    • 대한근관절건강학회:학술대회논문집
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    • 2001.04a
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    • pp.179-190
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    • 2001
  • The purpose of this study was to explore the effects of GEAP on pain, joint function, activities of daily living(ADL) and fatigue in chronic arthritis patients. The GEAP was held twice a week for 6 weeks for chronic arthritis patients at one university hospital in Seoul, Korea. Thirty four subjects completed the program, who were recruited at four times from September, 1999 to September, 2000. The effect of GEAP were evaluated as follows: Pain severity and number of painful joints ADL, fatigue were measured before and after the GEAP. In order to examine the joint flexibility and strengthening, the followings were measured: the extent of the upward arm reach in both sides(flexibility of shoulder), the ability to touch fingertips of the both hands in back pat and rub(flexibility of arm), the degree of range of motion (ROM) of both ankles in their dorsiflexion(flexibility of ankle) and plantarflexion with standing with toe(strengthening of ankle), and the degree of knee extension, and the grip strength. Paired t-test and Wilcoxon signed rank test were used for data analysis and the significance of the differences in the variables was examined to compare the data obtained before and after the GEAP. After the GEAP, followings were found: 1. Pain severity and number of painful joints was significantly decreased. 2. The flexibility of both shoulders and arms, knee, both ankle were significantly improved. 3. The strengthening of both arms was significantly improved, but the strengthening of ankle was not changed. 4. ADL was significantly increased. 5. Fatigue was significantly decreased. In conclusion, GEAP used in this study was clearly proved to be an effective exercise program to reduce pain and fatigue, to enhance joint function and ADL in people with chronic arthritis. It is suggested that the GEAP should be recommended as one of the useful and appropriate nursing interventions for chronic arthritis patients.

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A Study on the Development of Tube-to-Support Nonlinear Impact Analysis Model (튜브와 지지대 사이의 비선형 충격해설모델 개발에 관한 연구)

  • 김일곤;박진무
    • Journal of KSNVE
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    • v.5 no.4
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    • pp.515-524
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    • 1995
  • Tubes in heat exchanger of fuel rods in reactor core are supported at intemediate point by support p0lates or springs. Current practice is, in case of heat exchanger, to allow clearance between tube and support plate for design and manufacturing consideration. And in case of fuel rod the clearance in support point can be generated due to the support spring force relaxation. Flow-induced vibration of a tube can cause it to impact or rub against support plate or against adjacent tubes and can result in fretting-wear. The tube-to- support dynamic interaction is used to relate experimental wear data from single-span test rigs to real multi-span heat exchanger configurations. The dynamic interaction cna be measured during experimental wear tests. However, the dynamic interaction is difficult to measure in real heat exchangers and, therefore, analytical techniques are required to estimate this interaction. This paper describels the nonlinear impact model of DAGS(Dynamic Analysis of Gapped Structure) code which simulates the tube response to external sinusodial or step excitation and predicts tube motion and tube-to-support dynamic interaction. Three experimental measurements-two single span rods excited by sinusodial force and a two span rod impacted by a steel ball are compared from the simulation nonlinear model of DAGS code. The simulation results from DAGS code are in good agreement with measurements. Therefore, the developed model of DAGS code is good analytical tool for estimating tube-to-support dynamic interaction in real heat exchangers.

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The Effects of PACE Program on Self-efficacy, Pain and Joint Function in Korean Immigrant Elderly with Osteoarthritis. (PACE 프로그램이 퇴행성관절염 노인의 자기효능감과 통증 및 관절기능에 미치는 효과 - 미국이민 한국노인을 중심으로 -)

  • Sohng, Kyeong-Yae
    • Journal of muscle and joint health
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    • v.6 no.2
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    • pp.278-294
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    • 1999
  • The PACE (People nth Arthritis Can Exercise) is an exercise program developed by the Arthritis Foundation to improve muscle strength and joint flexibility for patients with arthritis. The purpose of this study was to explore the effects of PACE program on self-efficacy, pain, and joint function in the Korean immigrant elderly. The PACE program was held twice a week for 6 weeks for Korean immigrant elderly who had osteoarthritis. Twenty four subjects completed the program, who were recruited in two places : 10 elderly in a senior residential apartment, and 14 elderly in a senior center supported by Congregated Meal Program for Korean Elderly. Self-efficacy(Sherer et al., 1982), pain severity(by using Visual Analogue Scale), and number of painful joints were measured before and after the PACE program. To examine the joint flexibility and strengthening, the followings were measured : the extent of the upward arm reach in both sides(flexibility of shoulder), the ability to touch fingertips of the both hands in back pat and rub(flexibility of arm), the degree of range of motion(ROM) of both ankles in their dorsiflexion(flexibility of ankle) and plantarflexion with standing with toe(strengthening of ankle), and the degree of knee extension. Wilcoxon signed rank test was used for data analysis and the significance of the differences in the variables was examined to compare the data obtained before and after the PACE program. After the PACE, followings were found : 1. Self-efficacy was significantly increased. 2. Pain severity and number of painful Joints was significantly decreased. 3. The flexibility of both shoulders and arms were significantly improved, but the flexibility of knee was not changed. 4. The flexibility and strengthening of both ankle was significantly improved. In conclusion, PACE was clearly proved to be an effective exercise program to promote self-efficacy, to reduce pain, and to enhance joint function in the elderly with osteoarthritis. It is suggested that the PACE program should be recommended as one of the useful and appropriate nursing interventions for elderly with osteoarthritis.

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