• The Plant Pathology Journal
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• v.24 no.2
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• pp.143-151
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• 2008

To define the functions of the rpf genes in Xanthomonas oryzae pv. oryzae (Xoo), which regulates pathogenicity factors in Xanthomonas campestris pv. campestris (Xcc), marker-exchange mutants of each rpf gene were generated. When the mutants were inoculated on a susceptible cultivar, the lesion lengths caused by the rpfB, rpfC, rpfF, and rpfG mutants were significantly smaller than those caused by the wild type, whereas those caused by the rpfA, rpfD, and rpfI mutants were not. Several virulence determinants, including extracellular polysaccharide (EPS) production, xylanase production, and motility, were significantly decreased in the four mutants. However, the cellulase activity in the mutants was unchanged. Complementation of the rpfB and rpfC mutations restored the virulence and the expression of the virulence determinants. Expression analysis of 14 virulence genes revealed that the expression of genes related to EPS production (gumG and gumM), LPS (xanA, xanB, wxoD, and wxoC), phytase (phyA), xylanase (xynB), lipase (lipA), and motility (pitA) were reduced significantly in the mutants rpfB, rpfC, rpfF, and rpfG. In contrast, the expression of genes related to cellulase (eglxob, clsA), cellobiosidase (cbsA), and iron metabolism (fur) was unchanged. The results of this study clearly show that rpfB, rpfC, rpfF, and rpfG are important for the virulence of Xoo KACC10859, and that virulence genes are regulated differently by the Rpfs.

• The Plant Pathology Journal
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• v.29 no.4
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• pp.364-373
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• 2013

It has been known that most regulation of pathogenicity factor (rpf ) genes in xanthomonads regulates virulence in response to the diffusible signal factor, DSF. Although many rpf genes have been functionally characterized, the function of rpfE is still unknown. We cloned the rpfE gene from a Xanthomonas oryzae pv. oryzae (Xoo) Korean race KACC10859 and generated mutant strains to elucidate the role of RpfE with respect to the rpf system. Through experiments using the rpfE-deficient mutant strain, we found that mutation in rpfE gene in Xoo reduced virulence, swarm motility, and production of virulence factors such as cellulase and extracellular polysaccharide. Disease progress by the rpfE-deficient mutant strain was significantly slowed compared to disease progress by the wild type and the number of the rpfE-deficient mutant strain was lower than that of the wild type in the early phase of infection in the inoculated rice leaf. The rpfE mutant strain was unable to utilize sucrose or xylose as carbon sources efficiently in culture. The mutation in rpfE, however, did not affect DSF synthesis. Our results suggest that the rpfE gene regulates the virulence of Xoo under different nutrient conditions without change of DSF production.

• Journal of Korean Society for Atmospheric Environment
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• v.28 no.4
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• pp.423-434
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• 2012

This study was carried out to develop for the emission factor of greenhouse gas (GHG) from medium and smallscaled incineration facility using RPF which is considering as a part of renewable energy in UNFCC. The actual concentration of the exhaust gas and the fuel composition of RPF were measured for the calculation of GHG emission factor in RPF incinerators, and were compared with the IPCC guideline. The $CO_2$ and $N_2O$ emission factors by the actual concentration of exhaust gas were $2.3575{\pm}1.0070tCO_2/tRPF$ and $0.0014{\pm}0.0014tN_2O/tRPF$ respectively. Also, $CO_2$ emission factor by the RPF composition was $2.7057{\pm}0.0540tCO_2/tRPF$. The GHG emission factor per energy by the actual concentration was $83.0867{\pm}26.0346tCO_2e/TJ$ which showed higher consistency with the GHG emission factor ($80.3967tCO_2e/TJ$) of waste plastic in the IPCC guideline (2006b). The $CO_2$ and $N_2O$ emission factor calculated in this study is considered as a meaningful data for GHG emission factor of RPF incineration facility because of not being developed in ROK.

• 한국신재생에너지학회:학술대회논문집
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• 2007.11a
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• pp.609-612
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• 2007

The co-combustion of coal and RPF(Refuse Plastic Fuel) mixture has been experimented in a commercially operating CFB coal boiler and the emissions such as SOx, NOx, TSP and dioxine were measured at the stack. The experimented RPF was supplied by domestic RPF company that is commercially producing RPF pellet from the wasted plastics. Up to 15% of total coal was substituted to RPF and no trouble was happened during normal boiler operation. SOx and NOx concentration was reduced about $15{\sim}20$% and TSP(Total Suspended Particle) was drastically reduced about 30% during co-combustion. Dioxine concentration at mixing ratio of 7.5% was $0.0487ng{\sim}TEQ/Sm^3$ ($O_2$, 12%) that satisfied governmental emission regulation.

• Fire Science and Engineering
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• v.22 no.1
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• pp.99-104
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• 2008

It is important to understand thermal characteristic as a method to estimate the new materials, because spontaneous ignition characterized by causing combustion in the low temperature without ignition source. If can not find out the thermal characteristics of materials, it is frequent that causes of fires could not be found. The danger level of spontaneous ignition material should be estimated and by closely studying its thermal characteristic. However, RPF(Refuse Paper & Plastic Fuel) is a solid matter and getting increasesa year by year because it is an economy profit as alternative energy for limited fossil fuels. Some time RPF occur a fire in the cases of its production process and conservation. Therefore study for thermal stability and critical ignition temperature of RPF was so imperative that the experiment by means of Bombe Calorimeter, TG-DTA, MS80, SIT-II, and Wire Basket Test was implemented. As a result, RPF had a caloric value 26.4-28.3 MJ/kg, and its initial pyrolysis temperature was $192^{\circ}C$ at heating rate 2 K/min. With the result of analysis by MS 80 which is an instrument measuring microscopic calory, pure RPF not containing water has higher caloric value than RPF containing 20% water. Also, SIT-II which is an instrument of insulated auto-ignition was ignited by $118.5^{\circ}C$. This temperature is lower than that of Wire Basket Test. The critical ignition temperature was calculated by Frank-Kamenetskii equation can cause ignition at $80^{\circ}C$ when conserved in the height of 10 m by the standard of infinity slab.

• Steel and Composite Structures
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• v.43 no.1
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• pp.67-77
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• 2022

In the current study, ordinary design of Represstessed Pre-Flex (RPF) girder by classical beam theory and numerical model taking buckled shape into consideration were compared with field-survey data to find imperfections on the RPF girder before prestressing and after preflexion. It should be noted that the ordinary design do not consider deformed shape of steel girder in RPF beam. The deformed shapes of steel girder due to the incomplete fabrication that could be caused by self-weight, preflexion misalignment, existence of lateral bracing at mid-span and stiffness of reaction frame were found using a newly developed model which was verified against a deformation survey conducted on actual RPF girder in the field. The final observed deformed shapes of RPF after concrete shrinkage and before prestressing were classified into W, C and Unsymmetric shapes in regard to both survey and analytical results. The deformation survey showed negligible amount of unwanted deformation compared to the large size of the RPF girders. The shallower width of the bottom flange of steel girder caused amount of lateral torsional buckling under self-weight and preflexion thereby affecting the unwanted final overall shape of the RPF girders. However, it was found that the unwanted deformation of RPF girders by fabrication errors even though it is negligible compared to the size of the girder, caused unsymmetrical stress contours in concrete and additional tensile stress and raise some safety issues.

• The Plant Pathology Journal
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• v.30 no.3
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• pp.304-309
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• 2014

The rpf genes and $colS_{XOO1207}/colR_{XOO1208}$ were known to require for virulence of Xanthomonas oryzae pv. oryzae (Xoo). In Xoo KACC10331 genome, two more colS/colR genes, $colS_{XOO3534}$ (raxH)/$colR_{XOO3535}$ (raxR) and $colS_{XOO3762}/colR_{XOO3763}$ were annotated. The $colS_{XOO3534}/colR_{XOO3535}$ were known to control AvrXa21 activity and functions of $colS_{XOO3762}/colR_{XOO3763}$ were unknown in Xoo. To characterize the relationship between rpf and colS/colR genes, expression of colS/colR genes in Rpf mutants of Xoo were analyzed with quantitative reverse transcription PCR (qRT-PCR). Expressions of all three colS/colR genes increased in the rpfF mutant in which DSF synthesis is defective. Expression of $colS_{XOO1207}/col-R_{XOO1208}$, $colS_{XOO3534}/colR_{XOO3535}$ and $colS_{XOO3762}/colR_{XOO3763}$ increased 2, 2-7, 3-13 folds respectively. Expression of $colS_{XOO3534}$ and $colS_{XOO3762}$ also increased 2-4 folds in the rpfG mutant in which the signal from DSF is no longer transferred to down-stream. Expression of the other colS/colR genes was not significantly changed in the rpfG mutant compared to the wild type. Since RpfF and RpfG are responsible for DSF synthesis and signal transfer from DSF to down-stream to regulate virulence gene expression, these results suggest that the DSF and DSF-mediated signal regulate negatively three colS/colR genes in Xoo.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.206.2-206.2
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• 2010

현재 RPF 생산공정에서 생산된 RPF는 약 7,500~8,500kcal/kg 의 높은 열량을 지니고 있다. 이러한 특성으로 연소시 소각로 내부의 온도가 부분적으로 급격히 상승하여 적정온도조절이 어렵고, 로내 장치들의 내구성이 저하되는 등 문제가 발생하고 있다. 또한, RPF에 포함된 비교적 높은 농도의 염소 함량(0.8~1.8% wt)으로 인해 다량의 대기오염물질이 발생되는 단점이 나타나고 있다. 따라서, 이러한 문제점들을 개선하기 위해 RPF의 개질이 필요하며 본 연구에서는 슬러지와 RPF를 혼합하여 열량, 성형성, 염소함량등을 고려하여 최적의 혼합비율을 선정하였다. J하수처리장에서 발생하는 하수슬러지를 이용하여 5, 15, 25, 30, 35%를 RPF 성형공정에 혼합하여 실험하였다. 5% 혼합시 발열량은 약 6,300~6,800kcal/kg, 염소농도는 0.8~1.6%(wt), 15% 혼합시 발열량은 5,500~6,000kcal/kg, 염소농도는 0.7~1.4%(wt), 25% 혼합시 발열량은 5,200~5,900kcal/kg, 염소농도는 0.6~1.1%(wt), 30% 혼합시 발열량은 5,000~5,700kcal/kg, 염소농도는 0.6~1.0%(wt), 35% 혼합시 발열량은 4,800~5,200kcal/kg, 염소농도는 0.4~0.6%(wt)으로 나타났다. 각 혼합비율에서 관찰된 성형성은 5~25% 혼합까지는 성형된 RPF와 유사하게 일정한 크기 및 강도를 유지 할 수 있었으나, 25% 이상 혼합시 분말형태의 가루가 많이 발생되며 강도가 약해져 쉽게 부스러지는 문제점등이 나타났다. 연료의 개질 형태나 성형성등을 고려하였을 때 슬러지 혼합비율이 약 15~25% 정도가 최적 혼합비율인 것으로 나타났다.

• 한국연소학회:학술대회논문집
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• 2004.06a
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• pp.173-179
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• 2004

Combustion of refuse derived fuel(RDF) and refuse plastic fuel (RPF) was carried out in a lab-scale circulating fluidized bed. Experiment was investigated cold flow visualization. RDF was made by C & tech and RPF was made by KRS. The results include distribution of temperature in the combustion chamber, and concentrations of flue gas such as $O_2$, $CO_2$, CO, $NO_x$ and HCs Micro G.C(gas chromatograph) was employed to find out concentration of He Temperature distribution was different when RDF and RPF were burnt respectably. As air ratio became increased, $CO_2$, CO, and total of HCs emissions were decreased. According to the number of carbon atom of HCs, HC were classified as five kinds of HC.

• Proceedings of the KAIS Fall Conference
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• 2006.05a
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• pp.593-595
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• 2006

고유가 시대를 맞이하여 석유류를 대체할 연료의 필요성이 대두되고 있다. 한편 석유로부터 나오는 농가의 폐비닐이나, 산업폐기물 중 가전제품 및 산업용 폐플라스틱의 증가로 재활용에 대한 요구가 증대되고 있다. 일본 등에서는 이미 RPF를 연료로 사용하는 것이 보편화 되고 있어, 사람이 섭취하는 다이옥신의 98%는 음식, 1.5%는 대기 중에서 섭취하며, 소각장치에서 다이옥신류의 생성 억제 및 제거 기술은 이미 많이 확보되어 있다. RPF를 효과적으로 생산하고 열병합 발전소, 대형 보일러 시설, 작게는 농가의 대형 비닐하우스 등에 사용하기 위한 체계적인 연구가 필요하며, 본 논문에서는 W 기업의 플랜트 설비를 이용하여 폐플라스틱에 음식물류폐기물을 혼합한 RPF의 제조 및 연소특성을 중심으로 고찰하였다.