• Title/Summary/Keyword: rooting percentage

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Effects of Inoculation with Mycorrhizal Fungi, Pisolithus tinctorius and Glomus sp. on the Rooting of Quercus acutissima Carr. Cuttings at Various Ortet Ages (모래밭 버섯균(菌)과 Glomus 균근균(菌根菌)의 인공접종(人工接種)이 연령(年齡)이 다른 상수리나무에서 채취(採取)한 삽수(揷穗)의 삽목발근(揷木發根)에 미치는 영향(影響))

  • Kim, Jong Ju;Lee, Kyung Joon
    • Journal of Korean Society of Forest Science
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    • v.79 no.3
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    • pp.302-308
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    • 1990
  • This study was conducted to determine the effects of mycorrhizal inoculation and ortet ages on the rooting of Quercus acutissima cuttings. The cuttings taken from 12-week-, 2-year-, 4-year-, and 20-year-old plants were rooted in mid summer in the rooting medium (vermiculite 2 : peatmoss 1 by volume) with or without Pisolithus tinctorius (Pt) and Glomus sp. inocula and IBA under intermittent misting system in an open shed. The average percentages of rooting were about 82%, 49%, 29%, and 13% for cuttings taken from 12-week-, 2-year-. 4-year-, and 20-year-old seedlings, respectively. Pt inoculation, enhanced rooting of cuttings at all age classes, except 12-week-old seedlings, with the highest enhancement(22%) observed in cuttings taken from 20-year-old trees. The highest percentage of rooting in each age group eras 88.9% in 12-week-old seedlings treated with Pt plus 3.000ppm IBA, 75% in 2-Year-old plants with 1,000ppm IBA, 58.3% in 4-year-old plants with 3.000ppm IBA and 22% in 20-year-old plants. The addition of Glomus sp. fungus inoculum failed to enhance rooting. Pt mycorrhizal inoculation enhanced root dry weight, length, and diamter of adventitious roots at cuttings taken from 12-week- and 20-year-old trees, except the cuttings taken from 4-year-old seedlings. Rooted cuttings had more total nitrogen content in the leaves than unrooted cuttings, and the greater rooting response was associated with the higher phosphorus content in the leaves.

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Effect of Topophysis and Uniting Method of Rootstock and Scion on Rooting and Subsequent Growth of Stenting-propagated (Cutting-grafted) Roses (접수의 채취부위 및 접수와 대목의 고정법에 따른 장미 접삽묘의 생육 특성)

  • Park, Yoo-Gyeong;Jeong, Byoung-Ryong
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.456-461
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    • 2010
  • A study was conducted to investigate the effect of topophysis, and uniting method of rootstock and scion on rooting and subsequent growth of stenting-propagated cut rose ($Rosa$ $hybrida$ Hort.) in an effort to develop an efficient stenting propagation method for domestic rose cultivars. Four cultivars used in this study were two standard type cultivars 'Sweet Yellow' and 'Hanmaum', and two spray type cultivars 'Chelsi' and 'May'. Scions were grafted on cuttings of a rootstock $Rosa$ $indica$ 'Major'. The stenting-propagated scion-rootstock unions were planted in rockwool cubes ($50{\times}50{\times}50mm$, Delta, Grodan, Denmark) and were placed in a graft-take chamber for five days before being placed on misted greenhouse beds. The rootstock was removed of all leaves and nodes. Both the base of scions and top of stocks were simultaneously cut at a $45^{\circ}$ angle for grafting. Scions were prepared as single node cuttings, each with a five-leaflet leaf. Three positions of topophysis used were 7-9th (top), 4-6th (middle), and 1st-3rd (bottom) nodes from the stem base. Four uniting materials used were tube, tube + parafilm wrap, tube + clothespin, and clothespin. Rooting and growth were affected by the topophysis and cultivar. The best topophysis for rooting was 7-9th (top) nodes in all cultivars. Topophysis affected percent rooting, and number of roots, length of the longest root, and but not weight, shoot length and graft-take. Rooting and growth were affected by the uniting method and cultivar. Tube uniting method generally showed higher percentage graft-take, percent rooting, and number of roots than other methods. However, rootstock and scion union was not complete in this treatment. On the whole, the greatest rooting and subsequent growth of stenting-propagated plants were found in the tube + clothespin method. Except 'Sweet Yellow', rooting and growth were not adequate in the clothespin method. The results suggested that a tube + clothespin method was the most effective, and this method may be used as a substitute to save labor compared to a tube + parafilm wrap method which is currently being used in commercial nurseries.

Position Effect of Axillary Buds on Shoot Multiplication and Rooting in Bud Culture of Quercus acutissima (상수리나무 기내(器內) Axillary Bud의 치상부위(置床部位)에 따른 다경(多莖) 및 발근유도(發根誘道) 효과(効果))

  • Moon, Heung Kyu;Kim, Jae Hun;Park, Jae In
    • Journal of Korean Society of Forest Science
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    • v.76 no.4
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    • pp.370-375
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    • 1987
  • This study was conducted to examine the position effect of axillary buds on shoot multiplication and rooting with 1-year-old seedlings of Quercus acutissima. Shoot multiplication was greatly affected by axillary bud position: Productivity of multiple shoots were decreased in the order of top, mid and basal explants respectively. The best shoot multiplication (mean 6.1 shoots per explant) was obtained on WPM medium containing $1.0mg/{\ell}$ BAP and $0.1mg/{\ell}$ NAA using basal explant after 4-week-culture. Rooting was also greatly influenced by position. Its percentage was increased in the order of top, mid and basal explant respectively. Root initiation was better and more rapid on 1/2MS medium than GD medium. High rooting percentage (100%) was obtained on 1/2MS medium containing $0.2mg/{\ell}$ IBA after 15 days culture. Sucrose concentrations did not effect on rooting. However root development and shoot growth were greatly affected by them. Root was developed shortly on 1-2% levels and shoot growth was getting retarded, whereas both of them did not show significant difference at 3-6% levels. Rotting was decreased on 7-8% levels gradually, but shoot and leaf condition was better than any other concentrations. Survival rate of rooted explants in pot was varied according to the position of explants. Seedlings of top part were survived up to about 50% but most of mid and basal part seedlings did not survive over 4 weeks even in high humidity condition. Seedlings in pots showed normal growth over 10 months but most of them showed the condition of premature leaf shedding.

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Characteristics of Nursery Plants Influenced by Leaflet and Raising Method for Soft-Nodal cuffing in Cherry Tomato (토마토 절간(節間)을 이용한 마디삽목 시(時) 삽수의 절위(節位)와 삽수의 소엽(小葉)부착 유무가 묘(苗) 소질에 미치는 영향)

  • Yang, Seung Koo;Son, Dong-Mo;Choi, Kyung Ju;Kim, Sang Chaul;Kim, Wol-Soo;Chung, Soon Ju
    • Horticultural Science & Technology
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    • v.19 no.4
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    • pp.483-487
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    • 2001
  • This study was carried out to investigate the role of attached leaflet on rooting and growth by one nodal cutting in tomato. As cutting sources 4 to 6 nodes could be obtained from one nursery seedling. Medium for cutting was a mixture with perlite plus peat moss (1 : 1, v/v), and each plug capacity was 30mL in the 72 cells-plug tray. Plant height and the number of leaves were significantly increased by attached leaflet cutting in 'Pepe' and 'Coco' cherry tomato. Dry weights of top and root were increased as much as 3 to 15 times in the cutting attached leaflet. Rooting percentage was 93.5% in one node cutting and 86% in the cutting with hypocotyl node part. At 20 to 24 days after nodal cutting, healthy nursery plant could be produced to transplant in field. The nursery plants by nodal cutting (NPNC) showed more fibrous roots and less tap roots than that of seedling. In terms of rooting pattern, NPNC rooted at the whole ground stem part, while rooting of seedling occurred at basal part of hypocotyl.

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Effect of Length of Cutting, Number of Leaflets Attached and Cutting Position on Rooting and Growth of Cuttings of Rosa hybrida L. 'Red Sandra' and 'Little Marble' (삽수 길이, 소엽수 및 삽수 채취부위에 따른 절화장미 'Red Sandra'와 'Little Marble'의 발근과 생장)

  • Jeong, Jong-Woon;Hwang, Seung-Jae;Park, Su-Min;Jeong, Byoung-Ryong
    • Journal of Bio-Environment Control
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    • v.16 no.2
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    • pp.115-120
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    • 2007
  • An experiment was conducted to measure rooting and growth of cuttings of rose as affected by cutting length, number of leaflets and cutting position. Test plants were two cultivars of Rosa hybrida 'Red Sandra'(standard) and 'Little Marble'(miniature). Cutting length was 4, 7 or 10cm for 'Red Sandra' and 3, 5 or 7 cm for 'Little Marble'. The number of leaflets left on the five-leaflet leaf was 0, 2, 4 or 5. The cutting position on the stem was between $1{\sim}2,\;3{\sim}4,\;5{\sim}6\;or\;7{\sim}8$ nodes from the shoot tip, which have five leaflet leaves. The most efficient cutting length was 7cm in both cultivars. Treatment with all leaflets left and cutting position at $1{\sim}2$ nodes resulted in good rooting ratio for both cultivars.

Propagation by Cutting Method of Korea Rare Endemic Thuja koraiensis Nak. (희귀식물(稀貴植物) 눈측백나무(Thuja koraiensis Nak.)의 삽목증식(揷木增殖))

  • Song, Jeong-Ho;Lee, Jung-Joo;Koo, Yeong-Bon;Lee, Kab-Yeon;Han, Sang-Don;Yang, Byeung-Hoon
    • Journal of Korean Society of Forest Science
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    • v.95 no.4
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    • pp.393-397
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    • 2006
  • Thuja koraiensis Nak. is a short and creeping evergreen shrub which reaches about 3 m in height and only occurs in the northeast China and in high mountains over the Korea. It's designated as a rare and endangered tree species in Korea and DD (data deficient) in Red List Category & Criteria of IUCN. This study was carried out to develop the propagation technique by cutting for conservation of genetic resources of T. koraiensis. The rooting responses of branch cuttings, obtained from hard (May) and semi-hard wood shots (August) to three plant growth regulators (PGRs), namely, IAA, IBA, and NAA applied at various concentrations (0, 100, 500, 1000, 2000, and 3000 mg/l) were examined in sand and mixed soil media. Percentage of rooting showed significant difference between cutting time, among kinds and among concentration of PGRs. The optimum cutting time was April to May in hardwood cutting. The application of IAA 1000 mg/l and NAA 500mg/l were effective in callus formation and rooting of cutting. Relatively, rooting of cutting of the control taken in May was above 93%.

Adventitious Rooting of Cherry Dwarfing Rootstock 'Gisela 5' in Semi-hardwood Cuttings (체리 왜성대목 '기셀라 5' 반경지 삽목 발근 특성)

  • Kim, S.H.;Kim, H.L.;Kang, S.K.;Kwack, Y.B.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.21 no.1
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    • pp.5-13
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    • 2019
  • The first commercial planting of sweet cherry in Korea is thought to be done in early 1920s. The planting area of sweet cherry in Korea is about 500 ha in 2017. Although sweet cherry is considered a minor fruit in Korea, it is one of premium fruits in market and popular among children and women, especially among pregnant women. The import of sweet cherry fruits has increased dramatically in recent years. Seedlings of Prunus lannesiana or clones of 'Colt' (P. avium × P. pseudocerasus) were usually used as sweet cherry rootstocks in Korea. During recent decades new dwarfing rootstocks for cherry such as 'Gisela' series were developed in Germany. Among them, several 'Gisela' series have been mainly used in international nurseries. In this study, we investigated optimum rooting condition of a dwarfing rootstock 'Gisela 5' in summer season cuttings. Among eight soil conditions tested, saprolite + commercial nursery soil (1:1) and saprolite + vermiculite (1:1) showed higher rooting percentage than others, showing 93.6% and 88.9%, respectively. More than 95% of 'Gisela 5' rootstocks produce roots when it was treated with IBA for several seconds just before cuttings, irrespective of concentrations (500 to 2000 mg·L-1).

Studies on the Propagation of the Genus Osmanthus (목서류(木犀類)의 증식(增殖)에 관(關)한 연구(研究))

  • Youn, Ki Sik;Goo, Gwan Hyo;Jo, Chung Suk
    • Journal of Korean Society of Forest Science
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    • v.75 no.1
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    • pp.19-24
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    • 1986
  • Present studies were carried out to understand the way of propagation of Osmanthus which has much value as ornamental trees in southern parts of Korea. The results of these experiments were summarized as follows: 1. In order to increase the survival of Osmanthus, scions of Osmanthus was grafted on the stocks of Ligustrum obtusifolium S. et Z. The survival of the grafts were 63% for Osmanthus fragrans var. aurantiacus Makino and 60% for Osmanthus asiaticus Nakai. 2. The growth of the grafts was initiated from 28th day after the grafting and the height growth resumed three times, and the growth percentage was 63% for Osmanthus fragrans var. aurantiacus and 59% for Osmanthus asiaticus. 3. Cutting medium mixed with perlite and vermiculite (1:1) gave better results than either use of them separately, and the rooting percentage was 93% for Osmanthus fragrans var. aurantiacus and 90% for Osmanthus asiaticus. 4. Rooting percentage of cuttings was lower in hard-wood cutting than that of in green-wood cutting. 5. Grafts showed fairly good height growth in July and September, and ceased growth in October. 6. Correlation between height growth and diameter of root collar of grafts in case of Osmanthus fiagrans var. aurantiacus was highly significant and showed linear correlation ($r=0.70^{**}$)

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An Experimental Study on the Factors Affecting Damages of Submerged Rice Plants in Tong-il Variety Plots (II) -Emphasis on the Effects of Submergence Treatment on the Grain yields of Rice Plants- (통일계벼의 침수피해요인에 관한 실험적 연구(II) - 침수가 벼수량에 끼치는 영향을 중심으로 -)

  • 김철기;박명근
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.25 no.2
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    • pp.35-41
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    • 1983
  • This research is mainly to deal with the effects of submergence treatment on the grain yields of two rice plants, local variety, "Akibare" and Tongil line variety, "Milyang 23". The results obtained are summarized as follows. 1. According to the rice products of each plot the grain yield index was smallest in the plot treated at the early heading stage. The index of the next order became smaller in order of late flowering stage, late reduction division stage, milk ripe stage and dough ripe stage etc. The submerged stage at which the damages were smallest was tillering stage. Under the condition of two thirds or one third submerged depth of plant height, few differences in the grain yield index between Milyang 23 and Akibare was found, but except rooting stage, the damages of milyang 23 by whole submergence during growing period were mostly greater than those of Akibare. Especially the grain yield index of early heading stage at which the damages by whole submergence was most serious showed 45 percentage for one day submergence, 31 percentage for 3 days and 0.7 percentage for 7 days in Akibare plots, and 26.7% percentage for one day submergence, 7.9 percentage for 3 days and none for 7 days in Milyang 23 plots. 2. All the factors such as submerged stage, submerged depth and submerged period in this experimental test were highly recognized significance. The factors of the submerged depth and duration influenced on greater damages than the others. According to the difference in grain yield between plots, the larger the submerged depth and duration were, the larger the significant difference appeared. And between the treated levels at other submerged stages except both early tillering stage and most active tillering stage, the significance in the differences in grain yield was recognized, while only the submergence at early heading stage showed the most serious damages. 3. The decreased rate of grain yield for one day submergence at early heading stage indicated that in case of whole submergence of plant height it was 73 percentage in Milyang 23 plot and 55 percentage in Akibare plot, and in the event of two thirds and one third submergences of it, 20 percentage and 10 percentage in both Mulyang 23 and Akibare plots respectively. Therefore, the current criteria for planning project that restricted allowable submergence duration of more than 30cm submerged depth to 24 hours, should be amended not to exceed the submerged depth of 60cm when the duration of more than allowable submerged depth of 30cm will be limited to 24 hours, or within the limits of 12 hour submerged duration for locai variety and of less than 12 hour duration for Tong-illine variety as long as possible in case that submerged depth will be allowed to more than 60cm depth.ore than 60cm depth.

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Elimination of SPFMV from Virus-infected Sweet Potato Plants through Apical Meristem Culture

  • Kim, Young-Seon;Jeong, Jae-Hun;Park, Jong-Suk;Eun, Jong-Seon
    • Plant Resources
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    • v.7 no.3
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    • pp.200-205
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    • 2004
  • Sweet potato infected with a viral disease (SPFMV) showed irregular chlorotic patterns, so called feathering associated with faint or distinct ring spots that have purple-pigmented borders. SPFMV was eliminated from sweet potato plants using meristem tip culture. MS medium supplemented with BAP (2mg/L) and NAA (0.05 mg/L) was used for shoot proliferation and 1/2 MS medium for rooting of the plants. Highest percentage of regenerated plants (60%) was obtained from the optimum size (0.3-0.5mm) meristem tips. Of these, 60% plants were found negative for SPFMV by RT-PCR. Virus detection by RT-PCR was found to be a reliable method. Meristem-tip culture to produce SPFMV-free quality sweet potato and virus detection by RT-PCR is an efficient, time saving and reliable method for production of SPFMV-free tissue culture raised plants.

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