• The korean journal of orthodontics
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• v.51 no.4
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• pp.231-240
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• 2021

Objective: This outcome analysis study evaluated the actual positions of the orthodontic miniplate and miniplate anchoring screws (MPASs) and the risk factors affecting adjacent anatomic structures after miniplate placement in the mandibular incisal area. Methods: Cone-beam computed tomographic images of 97 orthodontic miniplates and their 194 MPASs (diameter, 1.5 mm; length, 4 mm) in patients whose miniplates provided sufficient clinical stability for orthodontic treatment were retrospectively reviewed. For evaluating the actual positions of the miniplates and analyzing the risk factors, including the effects on adjacent roots, MPAS placement height (PH), placement depth (PD), plate angle (PA), mental fossa angle (MA), and root proximity were assessed using the paired t-test, analysis of variance, and generalized linear model and regression analyses. Results: The mean PDs of MPASs at positions 1 (P1) and 2 (P2) were 2.01 mm and 2.23 mm, respectively. PA was significantly higher in the Class III malocclusion group than in the other groups. PH was positively correlated with MA and PD at P1. Of the 97 MPASs at P1, 49 were in the no-root area and 48 in the dentulous area; moreover, 19 showed a degree of root contact (19.6%) without root perforation. All MPASs at P2 were in the no-root area. Conclusions: Positioning the miniplate head approximately 1 mm lower than the mucogingival junction is highly likely to provide sufficient PH for the P1-MPASs to be placed in the no-root area.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.222-229
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• 2002

Ixeris dentata root were extracted with methanol and then fractionated with n-hexane, EtOAc and BuOH to get active fractions. and their antioxidant and antimicrobial activities in each fraction were determined. Ethyl acetate fraction of Ixeris dentata root showed strong antioxidant activities, but aqueous fraction did not show any activities. But in the antimicrobial test, aqueous fraction showed strong antimicrobial activities except to Escherichia coli. especially, aqueous fraction showed the strongest activities against Hypocrea nigricans. and butanol fraction showed the strongest activities against Cladosporium herbarum. This study was performed to determine the antimutagenic and cytotoxic effect of Ixeris dentata root methanol extract on Salmonella typhimurium TA98, TA100 and cancer cell lines using ames test and cytotoxicity assay, respectively. Cancer cell lines include human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma (Hep 3B). Futher fractionations with hexane, ethyl acetate, butanol and water from methanol extract of Ixeris dentata root were performed to obtain effective fraction, methanol extracts showed 79.94% inhibitory effect on the mutagenesis induced by N' -methyl- N' -nitro-N-nitrosoguanidine(MNNG) against TA100, while 89.99% inhibition was observed on the mutagenesis induced by 4-nitroquinoline-1-oxide(4NQO) against TA98. In the meanwhile, butanol fraction showed 89.92% and 71.01% inhibitory effect on the mutagenesis induced by benzo(a)pyrene(B(a)P) against TA98 and TA100, respectively. Ethyl acetate fraction showed the strongest effect against A549, MCF-7 and Hep3B at the same concentration compared to those of other fration.

• Journal of Crop Science and Biotechnology
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• v.11 no.2
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• pp.91-100
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• 2008

Melting curve analysis of fluorescently labeled DNA fragments is used extensively for genotyping single nucleotide polymorphism(SNP). Here, we evaluated a SNP genotyping method by melting curve analysis with the two probe chemistries in a 384-well plate format on a Roche LightCycler 480. The HybProbe chemistry is based on the fluorescence resonance energy transfer(FRET) and the SimpleProbe chemistry uses a terminal self-quenching fluorophore. We evaluated FRET HybProbes and SimpleProbes for two SNP sites closely linked to two quantitative trait loci(QTL) for southern root-knot nematode resistance. These probes were used to genotype the two parents and 94 $F_2$ plants from the cross of PI 96354$\times$Bossier. The SNP genotypes of all samples determined by the LightCycler software agreed with previously determined SSR genotypes and the SNP genotypes determined on a Luminex 100 flow cytometry instrument. Multiplexed HybProbes for the two SNPs showed a 98.4% success rate and 100% concordance between repeats two of the same 96 DNA samples. Also, we developed a HybProbe assay for the Rcs3 gene conditioning broad resistance to the frogeye leaf spot(FLS) disease. The LightCycler 480 provides rapid PCR on 384-well plate and allows simultaneous amplification and analysis in approximately 2 hours without any additional steps after amplification. This allowed for a reduction of the potential contamination of PCR products, simplicity, and enablement of a streamlined workflow. The melting curve analysis on the LightCycler 480 provided high-throughput and rapid SNP genotyping and appears highly effective for marker-assisted selection in soybean.

• Journal of the Korea Institute of Building Construction
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• v.18 no.5
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• pp.463-469
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• 2018

A currently used roof green system with multi layers has a low constructability. Therefore a new integrated waterproof & root barrier sheet and roof green box unit system was developed using steel plate fixture and cone type fixture by electromagnetic induction heating method. This study was proceeded to evaluate adhesion performance of two types of fixtures on Engineering PE, TPO, PVC sheet in a normal condition, repeated heating and cooling condition. As a result, adhesion load on Engineering PE sheet showed the highest value. The adhesion loads of steel plate fixture showed higher value as heating temperature was getting higher. However adhesion loads of cone type fixture showed opposite tendency. Regarding to the test conditions, test results of normal condition, repeated heating and cooling condition showed same value. The cone type fixture using butyl tape showed 7 times lower adhesion load than that of cone type fixture using electromagnetic heating and 28% lower adhesion load in a repeated heating and cooling condition than a usual condition.

• The korean journal of orthodontics
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• v.41 no.5
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• pp.361-370
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• 2011

Objective: Speedy surgical orthodontics (SSO), an innovative orthodontic treatment, involves the application of orthopedic forces against temporary skeletal anchorage devices following perisegmental corticotomy to induce movement of specific dental segments. Herein, we report the biological effects of SSO on the teeth and periodontal structures. Methods: Five beagle dogs were divided into 2 groups and their 6 maxillary incisors were retracted $en$ $masse$ by applying 500 g orthopedic force against a single palatal mini-plate. Retraction was performed without and with perisegmental corticotomy in groups I and II, respectively. All animals were killed on the 70th day, and their periodontal structures were processed for histologic analyses and scanning electronic microscopy (SEM). The linear distance between the third maxillary incisor and canine was used as a benchmark to quantify the retraction amount. Results: Retraction was markedly faster and retraction amount greater in group II than in Group I. Surprisingly, Group II did not show any root resorption despite extensive retraction, while Group I showed prominent root surface irregularities. Similarly, SEM showed multiple resorption lacunae in Group I, but not in Group II. Conclusions: SSO is an effective and favorable orthodontic approach for major en masse retraction of the maxillary anterior teeth.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.183-187
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• 1997

This study was carried out to find the growth characteristics and content of sarsasapogenin in different plant parts of Anemarrhena asphodeloides Bunge. Five native-cultivars were collected and evaluated for several agronomic traits. The collected native-cultivars were classified into two seed-attached peduncle and vegetative propagation types. Seed-attached peduncle lines were predominance of growth traits than vegetative propagation. For the content of sarsasapogenin in each part was investigated, BuOH extarct of 'timo' was developed on silica gel 60G plate using elution solvent($CHC1_2$: Methyl ethyl Ketone : EtOH = 11 : 2 : 0.5). The developed plate were examined using Dual Wavelength Zig - Zag using. Content of sarsasapogenin in main root and lateral root were respectively 1.67mg/g and 1.31mg/g.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2008.04a
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• pp.197-202
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• 2008

In this paper, a hybrid vibration-impedance approaches is newly proposed to detect the occurrence of damage, the location of damage, and extent of damage in steel plate-girder bridges. The hybrid scheme mainly consists of three sequential phases: 1) to alarm the occurrence of damage, 2) to classify the alarmed damage, and 3) to estimate the classified damage in detail. Damage types of interest include flexural stiffness-loss in girder and bolts-loose in supports. In the first phase, the global occurrence of damage is alarmed by monitoring changes in acceleration features. In the second phase, the alarmed damage is classified into subsystems by recognizing patterns of impedance features. In the final phase, the location and the extent of damage are estimated by using modal strain energy-based damage index method and root mean square deviation method. The feasibility of the proposed system is evaluated on a laboratory-scaled steel plate-girder bridge model for which hybrid vibration-impedance signatures were measured for several damage scenarios.

• Structural Engineering and Mechanics
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• v.9 no.3
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• pp.269-288
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• 2000

The governing differential equations for free vibration of multi-step orthotropic shear plates with variably distributed mass, stiffness and viscous damping are established. It is shown that a shear plate can be divided into two independent shear bars to determine the natural frequencies and mode shapes of the plate. The jk-th natural frequency of a shear plate is equal to the square root of the square sum of the j-th natural frequency of a shear bar and the k-th natural frequency of another shear bar. The jk-th mode shape of the shear plate is the product of the j-th mode shape of a shear bar and the k-th mode shape of another shear bar. The general solutions of the governing equations of the orthotropic shear plates with various boundary conditions are derived by selecting suitable expressions, such as power functions and exponential functions, for the distributions of stiffness and mass along the height of the plates. A numerical example demonstrates that the present methods are easy to implement and efficient. It is also shown through the numerical example that the selected expressions are suitable for describing the distributions of stiffness and mass of typical multi-storey buildings.

• The Journal of Korean Society for Radiation Therapy
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• v.19 no.1
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• pp.19-26
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• 2007

Purpose: To compare of the accuracy among various measurement procedures of HDR Brachytherapy, and to evaluate the clinical suitability and usefulness of alternative PMMA (polymethylmethacrylateplastics: $C_5H_8O_2$) plate phantom without any additional cost due to the purchase of measuring apparatus. Materials and Methods: We made a comparative study on three types of measuring systems: well type chamber, source calibration jig, and PMMA plate phantom. Farmer type chamber was used for source calibration jig method and PMMA plate phantom method. Measurement was done 5 times each in comparison with the measurement values from manufacturer. Measurement results from experiment were compared with that from the manufacturer which is offered with the source whenever a source is substituted by a new one and evaluate the accuracy of source activity. Results: As a consequence of Ir-192 source measurement using well type chamber, source calibration jig and PMMA plate phantom, RMS (Root Mean Square) values for the relative error are 0.6%, 1.57%, 2.1%, respectively, compared with the data from manufacturer. And the mean errors with standard deviation are given $-0.2{\pm}0.5%$, $0.97{\pm}1.23%$, $-0.89{\pm}1.87%$ respectively. Conclusion: From the results shown by the three types of measurement system (well type chamber, source calibration jig, and PMMA plate phantom), the measurement with well type chamber produced the best accuracy. It turns out that we can also use the alternative system of PMMA plate phantom clinically without purchasing any additional particular apparatus since the system does not exceed the recommendation of AAPM (American Association of Physicists in Medicine), which requires the error range of within ${\pm}5%$.

• Restorative Dentistry and Endodontics
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• v.32 no.2
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• pp.121-129
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• 2007

The purpose of this study was to assess the antibacterial effect of sodium dichloroisocyanurate (NaDCC), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) on Enterococcus faecalis and to evaluate and to compare the time-dependant antimicrobial effect of NaDCC with NaOCl and CHX in the root canal in vitro before and after instrumentation. Extracted Human single teeth were prepared by serial instrumentation technique. The samples were autoclaved and contaminated for 3 days with E. faecalis monocultures. The teeth were then divided into 4 groups Each group was irrigated and inserted with 2% NaOCl, 2% NaDCC, 2% CHX and steri)ized saline. After 6, 12, 24, 72h, and 1 week incubation, sterilized paper point was inserted into the root canal. Paper points containing root canal contents were then placed on the agar plate. And then each root cana) was prepared with #4 and #5 GG (Gates-Glidden) drill. The debris were collected in the sterilized microtube and the plates were incubated at $37^{\circ}C$ in an increased $CO_2$ atmosphere. After 24h incubation the growth of bacteria around the paper points were measured. NaOCl and NaDCC solution shows similar antimicrobial effect for E. faecalis at 6, 12, 24, 72h and 1 week. In centrol group, irrigated with sterilized saline, no antimicrobial effect was observed. The results are in agreement with other investigators, who have shown the bactericidal property and possibility of NaDCC as a root canal irrigation solution. Thus it seems that NaDCC solutions can be clinically applied into the root canal within 1 week after dilution.