• Archives of Reconstructive Microsurgery
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• 제21권1호
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• pp.50-55
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• 2012

Purpose: We reviewed 66 cases of the glomus tumors in the hands. The purpose of this study was to review the characteristic signs and symptoms of glomus tumors and the surgical outcomes. We made hypothesis that (1) our guideline for surgical excision are reliable and (2) our surgical technique is efficient and less harmful to the nail root. Materials and Methods: The clinical diagnosis was made with occasional radiological studies and surgical excision was performed according to our indications. Patients with electrical shock-like pain by touching (the essential symptom) and at least 2 of 4 clinical signs and symptoms (cold hypersensitivity, paroxysmal pain that radiated proximally, blue discoloration, nail deformity for dorsal tumor or a palpable nodule for pulp tumor) underwent surgical excision. Results: The duration of symptoms of the patients was about 5.3 years. No difference of prevalence was found in both hands, but the thumb and the middle finger were more affected than the other fingers. The mean follow up period was 2.4 years and none of the patients complained the same characteristic pain at the last visit. Conclusion: To the best of our knowledge, this report is the largest case series about glomus tumors in the finger tip. The clinical signs and symptoms are important to make a diagnosis of glomus tumors, and surgical excision is the treatment of choice.

• Korean Journal of Environmental Agriculture
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• 제18권4호
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• pp.361-365
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• 1999

This atudy was conducted to determine the growth characteristics and photosynthesis of soybean (Glycine max L. cv. Keumjongkongl) 30 day old seedling to 100mM NaCl concentration containing 1/2 Hoagland`s nutrient solution in sand culture. The nodule formation of root is not found perfectly with NaCl stress. The leaf dry matter weight (g/plant) of stressed plant is more reduction in 77% to control than any other characters. The water content (%) is tend to increase but water potential (MPa) is tend to decrease at NaCl stress. The chlorophyll content (SPAD) is tend to increase at growing leaf age of control but decrease at NaCl stress. The photosynthesis, stomatal conductance and transpiration are tend to decrease sharply at NaCl stress.

• Korean Journal of Microbiology
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• 제31권2호
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• pp.123-128
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• 1993

Genes for dinitrogenase reductase (nifH) and dinitogenase a subunit (nifD) were found to be located on 7.9 kb of EcoRI, 6.5 kb of Sail, 7.3 kb of HindlII and 4.4 kb of Pstl fragments of the genomic blot of Rhizobium sp. SNU003. a symbiotic strain from root nodule of Canavalia lineata. Nine recombinant phage nif-clones were selected from the genomic library constructed by using EMBL-3 BamHI arms of bacteriophage lambda. Among them. Rnif-6 had insert DNA of 15.3 kb. in which 7.6 kb of BamHI!SacI fragment contained nifHD region. Therefore, the 7.6 kb fragment was subcloned into pUC19 and partial restriction map was constructed. As the results, nifH and nifD were found to be located continuously on 4.5 kb of BamHI/BglIl in the genome of Rhizobium sp. SNU003 strain.

• Korean Journal of Soil Science and Fertilizer
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• 제8권1호
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• pp.37-43
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• 1975

Effect of inoculation trial on plant height and dry matter yield of seven alfalfa varieties (Medicago sativa L. var. Washoe, Ranger, Lahontan, Narragensett, Atlantic, Vernal, and Moapa 69) which are considered to be well adapted in these areas were conducted during 1973-1974 in a relatively poor soil at the field station in Kyung Hee University. Root nodule bacteria: Rhizobium meliloti (Noculator Northrup Kings Co. Ltd) was applied at a rate to provide 6 oz/30l of alfalfa seeds. Seven varieties were seeded in pots on May 31, 1973 and in second year, they were transplanted in field. 1. Dry matter yield and plant height of all inoculation plots were significantly greater than those of the no inoculation plots in all varieties. 2. In the 1973, dry matter yield of inoculation plot a in pot experiment was in the order of Moapa 69> Vernal> Lahontan> Washoe> Atlantic> Narragensett> Ranger. 3. There was no difference in dry matter yield between with and without-inoculation after transplanted in a field in the second year (1974). Total dry matter yield of varieties was in the order of Washoe> Lahontan> Moapa 69> Ranger> Narragensett> Vernal> Atlantic.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.955-959
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• 2003

Rhizobium tropici CIAT 899 is capable of synthesizing inactive apo-glucose dehydrogenase (GDH). To become an active holo enzyme, the GDH requires a cofactor, PQQ. When R. tropici CIAT 899 was grown in a broth culture medium containing hydroxyapatite and pyrrolo quinoline quinone (PQQ), pH decreased while the concentration of soluble P increased. The solubilization of hydroxyapatite was associated with the production of gluconic acid and 2-ketogluconic acids. The organic acid production and P solubilization were greatly enhanced when the bacterium was grown with air supply. Effect of R. tropici CIAT 899 with (CI＋PQQ) and without PQQ (CI) on the common bean growth was examined. Shoot and root weight, and N and P contents in CI＋PQQ treatment, were significantly higher than those in control and CI treatment. Nodule weight and acetylene reducing activities were also significantly higher in CI＋PQQ treatment than in other treatments.

• Korean Journal of Microbiology
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• 제30권1호
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• pp.30-36
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• 1992

nif (nitrogen fixation)-H.D, K genes of Frankia sp. SNU 014201. a symbiotic strain isolated from root nodule of Alnus hirsura, were found to be located in the genome on 13.5 kb of EcoRI, 18.0 kb of BamHI, 10.5 kb of BglII and 4.5 kb of KpnI fragments. Using EMBL-3 BamHI arms of bacteriophage lambda. the genomic library was constructed. from which fourteen recombinant phage nif-clones were selected. Among them, Ahnif-I2 had insert DNA of 18 kb, in which 7.9 kb of BamHl fragment contained nif-H, D, K and 3.6 kb of HindlIl/KpnI had nif-H and partial -D. Therefore, the 7.9 kb and 3.6 kb fragments were subcloned and partial restriction maps were constructed. As the results, nif-F1, D.K genes were found to be located continuously on the 6.5 kb of HindII/BamHI and 5.2 kb of SalIIBamHI fragment in the genome of Frankia sp. SNU 014201.

• Microbiology and Biotechnology Letters
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• 제19권3호
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• pp.222-227
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• 1991

Rhizobium fredii USDA193 is one of the causal organism for root nodule formation in soybean (peking). Previously we cloned the pectate lyase gene (SY1) of R. fredii USDA193. The $pel^-$ mutants (SY1$\Omega$ and SY1$\Omega$1) of SY1 were obtained using the in vitro insertional omega mutagenesis of RpelB (of Rhizobium pel) and fill-in reaction of RpelE (of Rhizobium pel) gene respectively, and we constructed two mutants (R, fredii USDA193$\Omega$ and R. fredii USDA193$\Omega$1) in pectate lyase function by marker-exchange with pe1B::$\Omega$ and R. fredii USDA193 strain (rif). The pectate lyase activity of two pel- mutant of R. fredii USDA193 was determined by spectrophotometric method. However, all pectate lyase activity of these mutants was not lost upon the mutagenesis by marker-exchange. This suggests that other pectate lyase genes may be present on the plasmid or the chromosome of R. fredii. As yet we do not have evidence linking RpelB and RpelE genes of R. fredii directly to the early nodulation process.

• Journal of The Korean Society of Grassland and Forage Science
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• 제23권2호
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• pp.81-90
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• 2003

This pot experiment was conducted to find out the effects of boron application(B$_{0}$; 0.0, B$_1$; 0.2, $B_2$;2.0, $B_3$;6.0, $B_3$;6.0, $B_4$;15.0me B/pot) on the forage performance in the pure and mixed swards of orchardgrass and white clover. This 1st part was concerned with the changes in the growth, flowers and flower buds, and roots/nodules of forages. The results obtained are summarized as follows: 1. At the $B_3$ and $B_4$ treatments, the B toxicity was more seriously in white clover than in orchardgrass in the first half of cutting orders, and reduced in the latter half. In white clover, it was more worsened in a mixture than in a pure culture. It was somewhat reduced at the best growth stage of each forage. 2. In orchardgrass, the B toxicity($B_3$,$ B_4$) showed the chlorosis on leaf tips, shallow leaf, little tillers, and weak stems. Whereas it showed the chlorosis/necrosis on old leaf edge, little and weak stolons in white clover. 3. Comparing with the B deficiency($B_{0}$ , $B_1$) and toxicity($B_3$, $B_4$), the optimum B application($B_2$) influenced markedly good growth of shoot, root, nodule, and flower (flower number, blooming period, early full flower) in white clover. 4. Comparing with orchardgrass, white clover was greatly influenced by the boron application. However, this responses of white clover to boron were reduced in a grass-clover mixture with additional fertilization. It was recognized that the good forage performance in a grass-clover mixture could be regulated by the adequate applications of boron and additional fertilizers.s.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제52권4호
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• pp.370-374
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• 2007

This study was carried out to investigate the soil chemical and physical properties by annul variation and yield properties according to cropping rotation system of paddy field which was cultivated rice and soybean of each 1 year by turns, each 2 year by turns, 1 year of rice and 3 years of soybean and 4 years of soybean continuously. This study was conducted for 3 years from 2002 to 2004 in National Institute of Crop Science(NISC), Korea. Study was controlled by Shinpaldal 2 of midmatured species, sow a field with soybean by $60{\times}15cm$ in 27 May. Chemical fertilizer was spread in the field by N 3.0 kg, $P_2O_5 $ 3.0 kg, $K_2O$ 3.4 kg per 10a. This experiment was carried for increase the degree of self sufficiency of soybean from detect the better cropping system in paddy field. The results of the study were as follows; Soil prosity was increased 17.4% in each one year rotation and 21.8% in the sector of cultivated soybean for four years. Results was indicated that $5{\sim}9cm$ of stem length, $0.5{\sim}1.0ea$ of branch number and $3.5{\sim}7.0$ of SPAD value was decreased in the sector of cultivated soybean for $3{\sim}4$ years compared to $1{\sim}2$ year cultivated sector. The soybean yield was reduced 12.9%(222 kg/10a) in the 2 year cultivated sector and 21%(201 kg/10a) in the 3 year cultivated sector compare with paddy-upland switching cultivation(255 kg/10a). Root nodule weight and number was tend to decrease according to the increasing duration of cultivated soybean.

• The Plant Pathology Journal
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• 제31권3호
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• pp.259-268
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• 2015

This study investigated the chemical characteristics and microbial population during incubation of four kinds of aerated compost teas based on oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR). It aimed to determine the effects of the aerated compost tea (ACT) based on MOVR on the growth promotion of red leaf lettuce, soybean and sweet corn. Findings showed that the pH level and EC of the compost tea slightly increased based on the incubation time except for rice straw compost tea. All compost teas except for oriental medicinal herbs and rice straw compost tea contained more ${NO^-}_3-N$ than ${NH^+}_4-N$. Plate counts of bacteria and fungi were significantly higher than the initial compost in ACT. Microbial communities of all ACT were predominantly bacteria. The dominant bacterial genera were analyzed as Bacillus (63.0%), Ochrobactrum (13.0%), Spingomonas (6.0%) and uncultured bacterium (4.0%) by 16S rDNA analysis. The effect of four concentrations, 0.1%, 0.2%, 0.4% and 0.8% MOVR on the growth of red leaf lettuce, soybean and sweet corn was also studied in the greenhouse. The red leaf lettuce with 0.4% MOVR had the most effective concentration on growth parameters in foliage part. However, 0.8% MOVR significantly promoted the growth of root and shoot of both soybean and sweet corn. The soybean treated with higher MOVR concentration was more effective in increasing the root nodule formation by 7.25 times than in the lower MOVR concentrations Results indicated that ACT could be used as liquid nutrient fertilizer with active microorganisms for culture of variable crops under organic farming condition.