• 한국수산과학회지
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• 제21권1호
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• pp.42-49
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• 1988

미역의 가공적성을 높일려는 목적으로 미역 다당류의 대부분을 차지하고 있는 알긴산의 물성을 이용한 미역쨈을 제조한 결과, 미역중량에 대해 $0.5\%\;K_2HPO_4$ 용액 4배량을 첨가할 경우 시중 딸기쨈과 유사한 점도를 가졌으며 유동특성은 항복력을 가지면서 의가소성형인 혼합형으로 간주되었다. 그리고 생미역으로 만든 쨈이 마른미역의 경우보다 점도가 좋았으며 미역 고유의 색조를 유지하는데도 양호하였다. 또 관능검사결과 부원료로서 사카린 $0.375\%$, 솔비톨 $2.0\%$, 구연산 $0.25\%$, 구운콩가루 $0.5\%$를 첨가하는 것이 양호하였다. 지방산조성에서는 고도불포화지방산인 $C_{18:3},\;C_{20:4},\;C_{20:5},\;C_{22:6}$의 함량이 상당히 높았으며 정미성분에 관여하는 유리아미노산 중 lysine, alanine, glutamic acid의 함량과 유기산중에서 구연산, 옥살산, $\alpha-ketoglutaric\;acid$, 젖산, 숙신산의 함량이 많았다.

• 한국식품영양과학회지
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• 제41권9호
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• pp.1226-1234
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• 2012

본 연구에서는 염증반응을 조절하는 다양한 신호전달체계를 중심으로 분자생물학적 방법을 통해 piceatannol의 항염증 기전을 규명하였다. LPS로 염증반응을 유도한 Raw 264.7 대식세포에서 piceatannol은 iNOS의 발현 억제를 통해 NO의 생성을 감소시키고 염증성 사이토카인(TNF-${\alpha}$, IL-6, IL-$1{\beta}$)의 생성을 감소시켰다. 염증반응을 조절하는 신호전달체계 중 piceatannol은 LPS에 의해 유도된 $I{\kappa}B$의 분해와 p65의 핵으로의 이동을 억제하고, LPS에 의해 유도된 SAPK/JNK의 인산화를 억제하였다. 또한 piceatannol은 LPS와 IL-6(LPS에 의해 증가됨)에 의한 STAT3의 활성화를 억제하였다. 뿐만 아니라 piceatannol은 Nrf2의 핵 내 축적을 야기하고 ARE의 transcriptional activity를 증가시켜 HO-1의 발현을 증가시켰다. 본 연구의 결과, piceatannol은 NF-${\kappa}B$와 AP-1, STAT3 신호전달의 억제를 통해, 그리고 HO-1의 발현 증가를 통해 항염증 효과를 나타내었다(Fig. 8).

• 한국식품영양과학회지
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• 제39권4호
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• pp.506-510
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• 2010

본 연구는 뇌혈관성 질환의 예방을 위한 양생약선으로의 활용을 위한 약용식물에 관한 연구의 일환으로 계획되었고 문헌상의 기록을 바탕으로 한 약용식물의 여러 기능 중 혈류 및 혈압 개선 효과에 중점을 두고 그 기능을 과학적으로 확인하여 보고자 하였다. 흰쥐를 대상으로 LDF를 이용하여 약용식물 추출물이 국소 뇌혈류량과 혈압에 미치는 효과를 관찰한 결과는 다음과 같다. 추출물을 여러 농도로 투여 시 국소 뇌혈류량은 증가되었다. 추출물이 국소 뇌혈류량을 증가시키는 기전을 확인하기 위해 atropine, L-NNA, methylene blue, indomethacin을 전 처리한 후 추출물을 투여하였을 때는 국소 뇌혈류량에 유의한 변화를 관찰할 수 없었으나 propranolol을 전 처리한 후 추출물을 1.0 mg/kg 농도 이상으로 투여했을 때는 국소 뇌혈류량이 유의적으로 증가함을 관찰할 수 있었다. 추출물을 여러 농도로 투여 시 혈압은 감소되었다. 추출물이 혈압의 변화를 초래하는지를 확인하기 위해 atropine, L-NNA, methylene blue, indomethacin을 전 처리한 후 추출물을 투여하였을 때는 혈압변화에 유의한 변화를 관찰할 수 없었으나, propranolol을 전 처리한 후 추출물을 1.0 mg/kg 농도 이상으로 투여했을 때는 혈압이 유의적으로 감소함을 관찰할 수 있었다. 결과에서 약용식물 추출물은 뇌혈류량을 증가시키고 혈압을 강하시키는 작용이 있음을 확인하였고 그 기전은 교감신경 $\beta$ 수용체의 활성을 변화시킴으로써 나타난 것으로 판단된다. 따라서 연구의 목적인 약선식으로의 활용에 있어 원재료로 사용이 가능하다고 판단되며 완제품에 대한 영양성분 및 유효성에 대한 연구를 수행할 때 기초자료가 될 수 있을 것으로 사료된다.

• Journal of Gastric Cancer
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• 제4권3호
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• pp.169-175
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• 2004

Purpose: The tumor suppressor gene p53 has been shown to be a factor in the carcinogenesis or progression of gastric cancer. The mutant p53 has been reported to cause a higher risk of lymph-node metastasis. Futhermore, mutation of the p53 has been linked to a poor prognosis for gastric cancer. The heat shock protein-27 (HSP27), a stress protein, has also been reported to be a poor prognostic factor in ovarian and breast cancers. However, in gastric-cancer patients, controversies exist as to its influence on the prognosis. In the present study, we used an immunohistochemical stain to observe the effects of p53 and HSP27 on the clinicopathological factors and on the prognosis for gastric-cancer patients. Materials and Methods: To evaluate the significance of p53 and HSP27 in gastric cancer patients, we analyzed 212 cases of gastric cancer (stage I.IV). Tissue samples of 212 patients were stained immunohistochemically for the mutant p53 protein and for HSP27. The correlations between protein expression and the clinicopathological factors were investigated. Results: The overall expression rates for p53 and HSP27 were $36.9\%\;and\;27.8\%$, respectively. p53 and HSP27 were correlated to each other because the HSP27 expression rate was higher in the p53-positive group (P=0.046). Statistically, the p53 and the HSP27 expression rates were significantly increased in the case of tumor invasiveness, lymphatic metastasis and vessel involvement. Therefore, they play a role in cancer progression. The 5-year survival rates of the p53-positive and the p53-negative groups were $62.8\%\;and\;60.1\%$, respectively (P=0.793) while the 5-year survival rates for the HSP27-positive and HSP27-negative groups were $54.2\%\;and\;63.1\%$, respectively (P=0.090). Conclusion: p53 and HSP27 were correlated to each other in our immunohistochemical study of gastric carcinomas and they were not independent prognostic factors in gastric- cancer patients. However, further studies are needed to determine their prognostic values for gastric-cancer patients.

• Journal of Acupuncture Research
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• 제19권6호
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• pp.111-124
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• 2002

A line of study reported that electroacupuncture(EA) modulate natural killer cell(NK Cell) activities. One report suggested that EA enhanced splenic interferon-gamma($IFN-{\gamma}$), interleukin-2(IL-2), and NK cell activity in Sprague-Dawley rats. Another study suggested that $IFN-{\gamma}$ mediates the up-regulation of NK cell activity, and endogenous ${\beta}$-endorphin secretion also play a role in the up-regulation of NK cell activity induced by EA stimulation. In order to better understand the molecular regulation underlying the activation of NK cell induced by EA, we have utilized cDNA microarray to elucidate how EA alters program of gene expression of spleen in rats. First, we divided three groups, group I was EA group treated with EA in restriction holder, group II was sham group with only holder stress, and last group III was control group with no treatment. We measured NK cell activity after EA stimulation three times for 2 days using $^{51}Cr$ release assay. Second, Biotin-labeled cDNA probes synthesized from EA group and sham group, were competitively hybridized to the microarray that contained variable genes. Such high-throughput screening has identified a number of EA-responsive gene candidates. Of these, we found that EA induced a subset of genes of genes that functionally could modulatory effects on NK cell activity. Genes(vascular cell adhesion molecule-1, protein-tyrosine kinase, CD94 mRNA) related to boost NK cell activity, were increased by EA And, genes(protein-tyrosine-phospatase mRNA, protein-tyrosine phosphatase(SHP-1) mRNA) related to inhibit NK cell activity, were decreased by EA. These EA-responsive genes may provide key insights from which to understand mechanisms of activation of NK cell induced by EA.

• 대한간호학회지
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• 제22권4호
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• pp.569-588
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• 1992

This study examined the relationship between the experience of stressful life events and somatic symptoms of urban women. Data were collected by interviewing 200 women from June to July 1, 1991. Vsable data work obtained from 162 of the women. Modified version of a stressful life events measurement tool developed p.5. Lee (1984) and the Somatic Discomfort Inventory by Wittenborn were used to measure the variables. Data were processed by an 5.p.5.5. program and analyzed. statistically for percentage, T-test, ANOYA and Pearson Correlation coefficient. Result of the Study are as follows : 1) The group total mean score of stressful life events was 92.66 $\pm$ 10.41. The higher scores in the $\boxDr$Test and school$\boxUl$ of the Extrapersonal factor, in the $\boxDr$Health problems$\boxUl$ of the Intrapersonal factor and $\boxDr$Conflict and differences within the family$\boxUl$ of the Interpersonal factor, factors. which a suggested by Neuman's model. 2) The group total mean score for somatic symptoms was 100.41$\pm$9.74. The higher scores were for the factors of $\boxDr$Fatigue (1.94)$\boxUl$, $\boxDr$Menopause (1.74)$\boxUl$, $\boxDr$Muscular system(1.67)$\boxUl$ and $\boxDr$Sleeping (1.67)$\boxUl$ 3) The mean scores of stressful life events were higher in the 40~60 age group, for middle school graduates(P＜.05), career women and those in nuclear families (P＞.05). 4) The mean scores of somatic symptoms were higher in the 45~60 age group, for middle school graduates, non career women and women with 5 or more children(P＜.05). 5) There was a positive correlation between the scores of stressful life events and somatic symptoms (r＝.585 P＜.05). The higher the level of stressful life events the higher the score of somatic symptoms, the results were consistent with the Extrapersonal, Intrapersonal and Interpersonal stress factors of Neuman's Health Care Systems. This research assessed the stressful life events of women, who play the most important role in the family for illness prevention and health promotion and suggested the importance of programs in the Primary Health Services to build basic coping resources.

• International Journal of Oral Biology
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• 제30권1호
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• pp.23-30
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• 2005

Bone remodeling is a process controlled by the action of two major bone cells; the bone forming osteoblast and the bone resorbing osteoclast. In the process of osteoclastogenesis, stromal cells and osteoblast produce RANKL, OPG, and M-CSF, which in turn regulate the osteoclastogenesis. During the bone resorption by activated osteoclasts, extracellular $Ca^{2+}/{PO_4}^{2-}$ concentration and degraded organic materials goes up, providing the hypertonic microenvironment. In this study, we tested the effects of hypertonicity due to the degraded organic materials on osteoclastogenesis in co-culture system. It was examined the cellular response of osteoblastic cell in terms of osteoclastogenesis by applying the sucrose, and mannitol, as a substitute of degraded organic materials to co-culture system. Apart from the sucrose, mannitol, and NaCl was tested to be compared to the effect of organic osmotic particles. The addition of sucrose and mannitol (25, 50, 100, 150, or 200 mM) to co-culture medium inhibited the number of tartrate-resistant acid phosphatase (TRAP) positive multinucleated cells induced by 10 nM $1{\alpha},25(OH)_2vitaminD_3$ ($1{\alpha},25(OH)_2D_3$). However, NaCl did exert harmful effect upon the cells in this co-culture system, which is attributed to DNA damage in high concentration of NaCl. To further investigate the mechanism by which hypertonicity inhibits $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis, the mRNA expressions of receptor activator of nuclear factor (NF)-kB ligand (RANKL) and osteoprotegerin (OPG) were monitored by RT-PCR. In the presence of sucrose (50 mM), RANKL mRNA expression was decreased in a dose-dependent manner, while the change in OPG and M-CSF mRNA were not occurred in significantly. The RANKL mRNA expression was inhibited for 48 hours in the presence of sucrose (50 mM), but such a decrement recovered after 72 hours. However, there were no considerable changes in the expression of OPG and M-CSF mRNA. Conclusively, these findings strongly suggest that hypertonic stress down-regulates $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis via RANKL signal pathway in osteoblastic cell, and may playa pivotal role as a regulator that modulates osteoclastogenesis.

• 한국융합학회논문지
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• 제9권6호
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• pp.287-298
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• 2018

본 연구는 제 6기 국민건강영양조사(2013-2015)의 원시자료를 이용한 2차 분석연구로, 초기성인의 동반식사 유무를 통한 정신건강 위험 요인을 파악하는데 그 목적이 있다. 본 연구는 제 6기 국민건강영양조사에서 20-30대 3,712명을 대상으로 일반적 특성, 식습관에 따른 정신건강을 파악하고자 교차분석을 하였고 초기성인층의 정신건강 위험요인을 파악하고자 다중 로지스틱 회귀분석을 실시하였다. 대상자의 개인적 특성들을 보정하였을 때, 남자는 아침식사 동반자가 있는 경우 우울감 여부, 저녁식사 동반자가 있는 경우 자살생각 여부가 낮게 나타났고, 여성은 아침식사 동반자가 있는 경우 스트레스 여부가, 점심식사 동반자가 있는 경우 자살생각 여부가 낮게 나타났다. 따라서 초기성인의 건강한 식습관과 긍정적 정신건강을 위해 함께 식사할 수 있도록 공공장소 및 기관의 공간 개선과 동반식사에 대한 필요성의 홍보가 부각되어야 할 것으로 생각된다. 그러므로 초기성인의 식습관관리 활동에 필요한 다각적인 프로그램을 제시하며 다양한 사람들과 함께하는 식습관 문화 교육을 통해 동반식사 할 수 있도록 노력이 필요할 것이다.

• 대한가정학회지
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• 제32권5호
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• pp.165-182
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• 1994

Korea's clothing industry which has been country's leading export industry and basic strategical industry is now faced with many difficulties both domestically and internationally. Domestically it is faced with continuing shortage of manpower in both production line and management high labour cost causing increase in price putting more weight on behavior of consumers resulting in change of industrial environment and continuing structural problems of industry itself. Internationally it is faced with strengthening of import regulations and protectionism of developed countries and rapid emergence of underdeveloped countries as leading exporting nations. In reality export plays the most essential role in our economy and is especially sensitive to the external environmental factors. Already economic bloc phonomenon can be seen everywhere and is continuing to accelerate in place such as E. U in Europ, North & South America as NAFTA, and South East Asian contries which recent tendency of economic unity effort is present. These countries of such economic blocs are imposing heavy custom duties reinforcing provision of country origin and acting out strict inspection regulations in order to protect the interest of their own industry. Therefore it is vital to manufacture excellent quality goods For these reasons study in this area has brought attention in Korea as well as worldwide in the recent years. Apparel industry which requires professional technology and ability is the most competitive international business. In order to challenge the international market the high level of intelligence is most required to produce high quality goods. The purpose of this study is to analyze the relationship between functions and roles of marketing and to approach problems in more efficient manner. Apparel industry is composed of various programs such as design pattern making merchandising and textile science. To succeed in the business is to give the highest satisfaction to the targeted market. Hence this study will example the factors that determine the Cost Quality and Performance of apparel products. The study will involve following steps; firstly establish relationship between the quality concept and productivity of apparel products Secondly inquire in to marketing strategy laying stress on apparel production related factors focusing on merchandising marketing production and operations Thirdly prospect 21st century apparel industry focusing on garment production and trade and also other countries structural improvement Fourthly establish the new dimension of competitive factors by grasping the actual circumstance of Korea's apparel industry in the international market. The research method will include; First reality approach method by analysing the present state of industry Second literal analysis such as marketing comparisons between leading apparel exporting countries.

• Tuberculosis and Respiratory Diseases
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• 제75권1호
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• pp.9-17
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• 2013

Background: In cancer cells, autophagy is generally induced as a pro-survival mechanism in response to treatment-associated genotoxic and metabolic stress. Thus, concurrent autophagy inhibition can be expected to have a synergistic effect with chemotherapy on cancer cell death. Monensin, a polyether antibiotic, is known as an autophagy inhibitor, which interferes with the fusion of autophagosome and lysosome. There have been a few reports of its effect in combination with anticancer drugs. We performed this study to investigate whether erlotinib, an epidermal growth factor receptor inhibitor, or rapamycin, an mammalian target of rapamycin (mTOR) inhibitor, is effective in combination therapy with monensin in non-small cell lung cancer cells. Methods: NCI-H1299 cells were treated with rapamycin or erlotinib, with or without monensin pretreatment, and then subjected to growth inhibition assay, apoptosis analysis by flow cytometry, and cell cycle analysis on the basis of the DNA contents histogram. Finally, a Western blot analysis was done to examine the changes of proteins related to apoptosis and cell cycle control. Results: Monensin synergistically increases growth inhibition and apoptosis induced by rapamycin or erlotinib. The number of cells in the sub-$G_1$ phase increases noticeably after the combination treatment. Increase of proapoptotic proteins, including bax, cleaved caspase 3, and cleaved poly(ADP-ribose) polymerase, and decrease of anti-apoptotic proteins, bcl-2 and bcl-xL, are augmented by the combination treatment with monensin. The promoters of cell cycle progression, notch3 and skp2, decrease and p21, a cyclin-dependent kinase inhibitor, accumulates within the cell during this process. Conclusion: Our findings suggest that concurrent autophagy inhibition could have a role in lung cancer treatment.