• Title/Summary/Keyword: rol

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Growth Characteristics of Transgenic Potato Using Wild-type Agrobacterium spp. (Agrobacterium spp. 에 의하여 형질전환된 감자조직의 생장특성)

  • 양덕춘
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.176-176
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    • 1998
  • This study was carried out to obtain the information for growth characteristics of crown gall tumor and hairy root transformed by Agrobacterium spp,. on the media with phytohormones, casein hydrolysate and activated charoal. Crown gall tumors and hairly roots were formed respectively on potato tuber discs infected by tumerfaciens A ch 5 and A.rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. PCR analysis of Rol C and Vir C gene fragments confirmed that crown gall root was prompted on the medium containing 2,4-D 2mg/l with casein hydrolysate lg/l. The survival ration of crown gall tumor callus derived from potato increased on medium containing the activated charcoal 0.5∼0.2mg/l because of the prevention, on the other hand, hairly roots were necrosis on the same medium. Callus derived from hairly root were excellently grown for a short time by suspension culture on liquid medium containing 2.4-d 2mg/L and casein hydrolysate lg/l.

Growth Characteristics of Transgenic Potato Using Wild-type Agrobacterium spp. (Agrobacterium spp. 에 의하여 형질전환된 감자조직의 생장특성)

  • 양덕춘
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.179-187
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    • 1998
  • This study was carried out to obtain the information for growth characteristics of crown gall tumor and hairy root transformed by Agrobacterium spp,. on the media with phytohormones, casein hydrolysate and activated charoal. Crown gall tumors and hairly roots were formed respectively on potato tuber discs infected by tumerfaciens A ch 5 and A.rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. PCR analysis of Rol C and Vir C gene fragments confirmed that crown gall root was prompted on the medium containing 2,4-D 2mg/l with casein hydrolysate lg/l. The survival ration of crown gall tumor callus derived from potato increased on medium containing the activated charcoal 0.5∼0.2mg/l because of the prevention, on the other hand, hairly roots were necrosis on the same medium. Callus derived from hairly root were excellently grown for a short time by suspension culture on liquid medium containing 2.4-d 2mg/L and casein hydrolysate lg/l.

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Evaluation of the usefulness of IGRT(Image Guided Radiation Therapy) for markerless patients using SGPS(Surface-Guided Patient Setup) (표면유도환자셋업(Surface-Guided Patient Setup, SGPS)을 활용한 Markerless환자의 영상유도방사선치료(Image Guided Radiation Therapy, IGRT)시 유용성 평가)

  • Lee, Kyeong-jae;Lee, Eung-man;Lee, Jeong-su;Kim, Da-yeon;Ko, Hyeon-jun;Choi, Shin-cheol
    • The Journal of Korean Society for Radiation Therapy
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    • v.33
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    • pp.109-116
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    • 2021
  • Purpose: The purpose of this study is to evaluate the usefulness of Surface-Guided Patient Setup by comparing the patient positioning accuracy when image-guided radiation therapy was used for Markerless patients(unmarked on the skin) using Surface-Guided Patient Setup and Marker patients(marked on the skin) using Laser-Based Patient Setup. Materials And Methods: The position error during IGRT was compared between a Markerless patient initially set up with SGPS using an optical surface scanning system using three cameras and a Marker patient initially set up with LBPS that aligns the laser with the marker drawn on the patient's skin. Both SGPS and LBPS were performed on 20 prostate cancer patients and 10 Stereotactic Radiation Surgery patients, respectively, and SGPS was performed on an additional 60 breast cancer patients. All were performed IGRT using CBCT or OBI. Position error of 6 degrees of freedom was obtained using Auto-Matching System, and comparison and analysis were performed using Offline-Review in the treatment planning system. Result: The difference between the root mean square (RMS) of SGPS and LBPS in prostate cancer patients was Vrt -0.02cm, Log -0.02cm, Lat 0.01cm, Pit -0.01°, Rol -0.01°, Rtn -0.01°, SRS patients was Vrt 0.02cm, Log -0.05cm, Lat 0.00cm, Pit -0.30°, Rol -0.15°, Rtn -0.33°. there was no significant difference between the two regions. According to the IGRT standard of breast cancer patients, RMS was Vrt 0.26, Log 0.21, Lat 0.15, Pit 0.81, Rol 0.49, Rtn 0.59. Conclusion:. As a result of this study, the position error value of SGPS compared to LBPS did not show a significant difference between prostate cancer patients and SRS patients. In the case of additionally performed SGPS breast cancer patients, the position error value was not large based on IGRT. Therefore, it is considered that it will be useful to replace LBPS with SGPS, which has the great advantage of not requiring patient skin marking..

Efficient Watercolor Painting on Mobile Devices

  • Oh, Junkyu;Maeng, SeungRol;Park, Jinho
    • International Journal of Contents
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    • v.8 no.4
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    • pp.36-41
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    • 2012
  • We present a novel watercolor painting drawing system which can work even on low powered computing machine such as tablet PC. Most digital watercolor systems are generated to perform on desktop, not low powered mobile computing system such as iPad. Our system can be utilized for art education besides professional painters. Our system is not a naïve imitation of real watercolor painting, but handles with properties of watercolor such as diffusion, boundary salience, and mixing of water and pigment.

Dynamic Modeling and Analysis of Control Systems for Skin Pass Mill (조질 압연기의 동적 모델링과 제어시스템 분석)

  • 이규택;이원호
    • 제어로봇시스템학회:학술대회논문집
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    • 2000.10a
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    • pp.316-316
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    • 2000
  • SPM dynamic model was developed by using Bland & Ford formulas considered elastic zone in roll gap, gauge meter equation, tension equation, speed equation and actuator models. And SPM controllers of the field were done model ing. It was shown the efficiency of constant tension, rol1ing force and elongation controllers by the simulation program and it was recommended the proper gain to the controllers of the field.

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Selection of Active Grow Hairy Root Lines in Ginseng (고생장 인삼 모상근의 선발)

  • 양덕춘;김용해;양덕조;민병훈;신성련;최광태
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.6
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    • pp.525-530
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    • 1998
  • These studies were carried out to select the active grow hairy root lines induced from various ginseng(Panax ginseng C. A. Meyer) parts. Hairy roots were induced in root explants, stem and petiole in vitro by A. rhizogenes R1000 or A. rhizogenes $A_4$. These hairy roots could be grown on the phytohormone free medium, and PCR analysis of rol C and vir C gene fragments confirmed that hairy roots were transgenic tissues. We have selected 11 hairy root lines with active growing characters among 300 hairy root lines selected based on growth and morphological characteristics on 1/2MS solid media with 250 mg/L carbenicillin. Morphological characteristics of selected 11 hairy root lines were thickness and thiness of main roots, and many projection for lateral roots, active grow of lateral roots. Among selected 11 hair root lines prominent characteristics of hairy roots with active growing characters were thiness of main roots and active grow of lateral roots. But characteristics of low growing hairy roots were thickness of main roots and low grow of lateral roots. Finally we have selected actively growing hairy roots, KGHR-1, KGHR-5, KGHR-8 among 11 hairy root lines.

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Constituents of Spiraea prunifolia var. simpliciflora (조팝나무 뿌리의 성분 연구)

  • Lee, Eun-Hee;Chung, Soon-Ok;Kim, Chong-Won;Woo, Mi-Hee
    • Korean Journal of Pharmacognosy
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    • v.27 no.4
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    • pp.389-396
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    • 1996
  • Spiraea prunifolia var. simpliciflora (Rosaceae) is a deciduous. latifoliate shrub growing in most parts of Korea. The roots of this plant have been used for malaria, as antipyretics and emetics. From the roots of this plant, sterol glycoside and two triterpenoids were isolated and the structures were elucidated by chemical and spectroscopic methods. They were identified as $3{\beta}-hydroxyurs-12-ene-28-oic$ acid (ursolic acid.1), $2{\alpha}$, $3{\beta}$, $19{\alpha}-trihy-droxyurs-12-ene-28-oic$ acid (tormentic acid.2) and ${\beta}-sitoste-rol-3-O-{\beta}-D-glucopyranoside$ (3).

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