• The KSFM Journal of Fluid Machinery
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• v.8 no.1 s.28
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• pp.16-22
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• 2005

This paper presents the method of ROL(Rate Of Loading) improvement for the Motor Operated Gate Valve operated in high differential pressure condition. ROL is one of the most important evaluation parameters for the valve ability. It is close to correlation in stem factor (SF) and appears different value by the differential pressure of fluid. ROL and SF are analyzed by the static test and dynamic test. The obtained result show that the modification of stem factor is very important factor for the ROL improvement. In order to obtain the same value of SF between static and dynamic test, stem and stem nut should be combined appropriately by the repetition test.

• Molecules and Cells
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• v.44 no.8
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• pp.613-622
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• 2021

In vertebrate eyes, the retinal pigment epithelium (RPE) provides structural and functional homeostasis to the retina. The RPE takes up retinol (ROL) to be dehydrogenated and isomerized to 11-cis-retinaldehyde (11-cis-RAL), which is a functional photopigment in mammalian photoreceptors. As excessive ROL is toxic, the RPE must also establish mechanisms to protect against ROL toxicity. Here, we found that the levels of retinol dehydrogenases (RDHs) are commonly decreased in phosphatase tensin homolog (Pten)-deficient mouse RPE, which degenerates due to elevated ROL and that can be rescued by feeding a ROL-free diet. We also identified that RDH gene expression is regulated by forkhead box O (FOXO) transcription factors, which are inactivated by hyperactive Akt in the Pten-deficient mouse RPE. Together, our findings suggest that a homeostatic pathway comprising PTEN, FOXO, and RDH can protect the RPE from ROL toxicity.

• 유체기계공업학회:학술대회논문집
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• 2004.12a
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• pp.166-171
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• 2004

The Rate-of-Loading(ROL) effect of motor operated gate valves causes a decrease in the thrust delivered at a specific torque by the actuator under dynamic flow ${\Delta}$P conditions as compared to the no-flow, static conditions. This effect needs to be studied for the quantitative evaluation of motor operated gate valve operability. This study is performed to verify the validity for the application of ROL in the evaluation of operability of motor operated gate valves for the opening stroke. The ROL is assessed on the basis of in-situ test data for the opening and closing strokes. The results show that the distribution of the ROL for the operling sake has a tendency to the negative value when DP is higher than 150psid.

• 유체기계공업학회:학술대회논문집
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• 2003.12a
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• pp.562-567
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• 2003

This paper presents the method of Rate of Loading(ROL) improvement for the Motor Operated Gate Valve operating in high differential pressure condition. The character of ROL and Stem Factor is analyzed. Static test and dynamic test were performed and acquired the diagnosis signal for the valve closing stroke. The result of this study is the modification of stem factor is very important factor for the ROL improvement. In order to obtain the same value of dynamic test thread friction coefficient stem and stem nut should be combined appropriately.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.329-334
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• 1995

Transformation system of rolC gene, dwarf gene in Lycium chinenese Mill. established by using system. Pin-punctured leaves induced numerous adventious buds in abaxial side when cultured on 3/2 MS medium containing 2.0 mg/L zeatin. Survival rate and shoot regeneration frequency of leaf explants decreased as kanamycin sulfate level increased. Shoot buds were not regenerated on 3/2 MS medium containing 10 mg/L kanamycin sulfate and 2.0 mg/L zeaein. Of the level tested, 10 mg/L of kanamycin sulfate was optimum in selection of kanamycin sulfate resistant plant. Co-culture time of bacteria and leaf explants was affected at the frequency of shoot regeneration and survival of leaf explants. Leaf explants co-cultivated during above 48hr severely decreased survival rate and shooting rate. Best result on survival rate and shooting rate were obtained when exposed for 24 h. 80 explants of 105 leaf explants survived on 3/2 MS medium containing 2.0 mg/L zeatin and 10 mg/L kanamycin sulfate, and 15 shoots was regenerated on the same medium. To select kanamycin sulfate resistant plant, regenerate as cultured on 3/2 MS medium containing 10 mg/L kanamycin sulfate, and obtained 5 kanamycin resistant plants. Southern blot analysis conformed that the rolC gene was incorporated into the genomic DNA of kanamycin resistant plants.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.482-487
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• 2009

The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26 produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.269-272
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• 1997

Cotyledonary petioles of Brassica napus cocultivated with Agrobacterium vectors for 72 h were transferred to MS medium with 0.5 mg/L NAA, 2.0 mg/L BA, 30 mg/L kanamycin, 100 mg/L cefotaxime, 30 g/L sucrose, 3 mg/L $\textrm{AgNO}_{3}$ and 2 g/L Gelrite. The cotyledonary petioles with green shoots were selected at a frequency of 17.5% in a selection medium and then rooted. Southern blot analysis confirmed the rolC and NPT IIgenes were incorporated into the regenerated plants. The stable inheritance of rolC gene was confirmed in progeny test of transgenic plants.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.21-26
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• 1999

These experiments were attempted to introduce rolC gene in the Petunia hybrida cv. Titan white by Agrobacterium mediated. The maximum frequency of shoot regeneration was obtained by 60% on MS medium containing 1.0 mg/L BA, 0.1 mg/L NAA, 200 mg/L kanamycin, 500 mg/L carbenicillin, 30 g/L sucrose, and 8 g/L agar. Kanamycin-resistant calli were selected from petunia leaf discs by cocultivation with Agrobacterium suspension cultures on MS medium. The addition of AgNO$_3$ and KMnO$_4$ in the medium increased the shoot regeneration by 31.3% from leaf disc as compared with non-treated leaf disc. Among clones exhibiting kanamycin resistance, only 3 clones were confirmed by southern hybridization analysis.

• Proceedings of the Korean Nuclear Society Conference
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• 1999.10a
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• pp.76.2-76
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• 1999

• Toxicological Research
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• v.26 no.1
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• pp.61-66
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• 2010

Retinoids have many beneficial effects on dermatological applications. But, retinoids cause skin irritation. In this study, the safety of retinoids was clarified via both primary skin irritation test in rabbits and sensitization study using an integrated model for the differentiation of chemical-induced allergic and irritant skin reaction (IMDS), an alternative method to sensitization test. The effects of retinoids on the change of ultraviolet A (UVA)-induced matrix metalloproteinase-1 (MMP-1) in human skin fibroblasts and the modulation of type-1 pN collagen synthesis in hairless mice were examined to clarify the anti-wrinkle effects. Alltrans retinol (t-ROL) and its derivative, all-trans retinoic acid (t-RA), showed mild skin irritation but did not induce the sensitization. t-ROL and t-RA exerted anti-wrinkle effects by inhibiting the UVA-induced MMP-1 in human skin fibroblasts and increasing the type-1 pN collagen synthesis in hairless mice. These findings suggest that retinoids do not induce the allergy, and show anti-wrinkle effects by decreasing MMP-1 activation and increasing collagen synthesis.