• KSBB Journal
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• 제13권6호
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• pp.649-655
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• 1998

Hantaviruses are rodent hosts-borne viruses belonging to the family Bunyaviridae, and are etiologic agents for two acute diseases, i.e., Haemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome (HPS). There have been a lot of reports on prophylactic vaccines and diagnostics for the diseases, but most of viral antigens have been prepared by eukaryotic cell culture. Nucleocapsid proteins of Hantaviruses are known as the major viral antigens. Thereby, we prepared nucleocapsid genes of Hantaan virus and Seoul virus by RT-PCR and cloned into plasmid vectors, pET-3a and pKK223-3. Both genes were expressed in Escherichia coli with higher expression level of Seoul viral nucleocapsid protein compared to that of Hantaan in pET-3a. Hantaan viral gene was expressed much higher level in plasmid pET-3a that in pKK223-3. About 30% of expressed nucleocapsid protein was soluble and the rest was remained in insoluble fraction.

• 한국동물학회지
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• 제37권2호
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• pp.255-261
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• 1994

Recombinant human pBrathyriod hormone related peptide (1-341 (rhPTHrP) has been known to stimulate bone resorption in intact bone tissue culture system. Osteoclast has been known as a primary responsible cell for bone resorption. To examine the effect of rhPTHrP on this cell, we employed disaggregated rat osteodast culture. As a result, we found that rhPTHrP sisnificBntly elevates both the number and total area of resorbed pits in this culture. On the other hand, the conflicting results between disagsregated rat osteoc13st culture and Ca2+-deficient hen osteoclast culture system have been a big obstacle for the progress of bone research. To verify the differences between rat 3nd chick osteoclast system, we performed the same experiment using chick embryonic osteoclast. Since the similar results were obtained from the disaggregated chick osteoclast culture, the discrepancy between chick and rat osteoclast culture study seemed to be due to the difference in culture method, rather due to the species-difference.

• 대한임상독성학회지
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• 제5권2호
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• pp.131-134
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• 2007

The emergence of anticoagulant rodenticides in the 1950's resulted in an epochal reduction in the use of all other agents. It is estimated that anticoagulant rodenticides constitute 95 percent of all rodenticides now in use. However, the advent of rodent resistance to these agents has led to a return to use of some other non-anticoagulant types of rodenticides. Older non-anticoagulant rodenticides have been stored in basements, garages, and barns and are still in use in some regions of the United States and in other countries. We report a case of non-anticoagulant rodenticide (beta-fluoroethyl acetate) poisoning.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1994년도 하계학술대회 논문집 C
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• pp.1643-1645
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• 1994

It has been reported frequently that damage to underground electric power cable was brought about, due to attack by Insect such as termite (white-ant) and by rodent such as rat, in tropical and semitropical areas. Among these cable damages, we are likely to describe the damage to cable by termites as causing the severe trouble in cable operation and to solve this trouble with termite-proof method regarded as the most effective and economical one. This report covers our experiences of manufacturing the cable for anti-termite purpose and its intrinsic properties in cable operation.

• 대한의생명과학회지
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• 제9권2호
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• pp.99-103
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• 2003

Hantavirus are rodent-borne RNA virus that belongs to the family Bunyaviridae. Those viruses persistently infect a variety of rodents, and are transmitted by aerosols of their urine, feces and saliva. Antibody titers of sera obtained from normal laboratory rodents against hantaviruses were investigated by indirect immunofluorscence antibody technique (IFA), Seroconversion rates of normal laboratory rodents showed higher in rats than that from hamster and mongolian (M). gerbil. Theses rates of normal laboratory rodents also showed higher in titers against puumala virus (PUUV) than in hantaan (HTNV) and seoul virus (SEOV). We are concerned about infections caused by hantaviruses, especially by PUUV, occurred in laboratory rodents.

• Journal of Korean Neurosurgical Society
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• 제65권2호
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• pp.161-172
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• 2022

Although technological advances and clinical studies on stem cells have been increasingly reported in stroke, research targeting hemorrhagic stroke is still lacking compared to that targeting ischemic stroke. Studies on hemorrhagic stroke are also being conducted, mainly in the USA and China. However, little research has been conducted in Korea. In reality, stem cell research or treatment is unfamiliar to many domestic neurosurgeons. Nevertheless, given the increased interest in regenerative medicine and the increase of life expectancy, attention should be paid to this topic. In this paper, we summarized pre-clinical rodent studies and clinical trials using stem cells for hemorrhagic stroke. In addition, we discussed results of domestic investigations and future perspectives on stem cell research for a better understanding.

• Journal of Veterinary Science
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• 제21권1호
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• pp.1.1-1.4
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• 2020

Tumor incidence in wild mammals is reportedly very low. Wild nutria, a large rodent, is known to carry many infectious diseases, but rarely exhibits neoplastic diseases. We necropsied a male wild nutria and found a large nodular mass in the left inguinal region, adjacent to the penis. Histopathologically, the mass was diagnosed as preputial gland adenoma. Spontaneous preputial gland adenomas are extremely rare in all animals. Moreover, reports of tumors in nutrias have been limited to adenocarcinomas of the lungs and uterus, as well as subcutaneous fibromas. Here, we describe preputial gland adenoma in a wild nutria.

• 대한이식학회지
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• 제28권3호
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• pp.113-120
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• 2014

Two-signal models are useful in explaining various types of immune responses. In particular, secondary, so-called costimulatory, signals are critically required for the process of T-cell activation, survival, differentiation, and memory formation. Early studies in rodent models showed that targeting T-cell costimulatory pathways elicits immunological tolerance, providing a basis for development of costimulatory therapeutics in allograft rejection. However, as the classic definition of T-cell costimulation continues to evolve, simple blockade of costimulatory pathways has limitations in prevention of allograft rejection. Furthermore, functions of costimulatory molecules are much more diverse than initially anticipated and beyond T cells. In this mini-review, we will discuss CD137-CD137L bidirectional signals as examples showing that two-signals can be applicable to multiple phases of immune responses.

• 한국환경생태학회지
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• 제34권3호
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• pp.198-206
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• 2020

본 연구는 지리산국립공원 세석평전 일대 설치류에 의한 수목피해실태를 조사하고, 피해목 주변에서 확인된 설치류 배설물 분석을 통하여 가해 의심종을 확인하고자 하였다. 피해목 조사는 대상지 내 탐방로를 중심으로 접근과 육안관찰이 가능한 지역에 한해 수종에 관계없이 실시하였으며, 가해 의심종 확인을 위해 피해목 주변 설치류 배설물에 대한 미토콘드리아 DNA Cyt-b의 염기서열 분석을 수행하였다. 설치류에 의한 피해목은 총 52개체로 모두 구상나무로 확인되었으며, 구상나무가 높은 밀도로 군락을 형성하고 있는 곳에서 집중되어 있었다. 수고 1.5m 이상 구상나무가 40개체가 조사되었으며, 이중 39개체는 피해 입은 가지부만 고사한 것으로 나타나 수목 전체에 영향을 미치지 않았다. 수고 1.5m 미만 구상나무는 12개체가 조사되었으며, 이중 7개체는 완전히 고사한 것으로 나타나, 상대적으로 어린 구상나무가 피해를 입을 경우 고사 가능성이 높은 것으로 나타났다. 배설물의 유전자 염기서열 분석결과 구상나무를 가해한 종은 대륙밭쥐로 확인되었다.

• 한국발생생물학회지:발생과생식
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• 제11권1호
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• pp.13-20
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• 2007

암컷 포유동물의 노화 과정에서 생식계는 체내 여러 시스템 가운데 가장 먼저 기능 저하가 나타난다. 생식 노화(reproductive aging) 과정 중인 암컷 포유동물은 비록 종 특이성이 있지만 주기성(cyclicity)의 소실과 같은 생식 능력의 감소와 함께 여러 기능들의 변화가 동반되면서 궁극적으로 인간의 폐경 현상과 같은 생식 능력 상실이 나타난다. 본 증설은 암컷 포유동물의 생식호르몬 축, 특히 신경내분비 회로의 노화에 대한 정보들과 노화 연구에 유용한 설치류 모델들을 소개하고자 한다. 암컷 설치류의 중년(middle age, 생후 $8{\sim}12$개월)은 인간의 폐경기 진입 직전에 해당되는 시기로, 이 시기에 시상하부 GnRH 분비의 맥동성과 급등(surge)이 현저히 지연되기 시작하고, 곧 이어 뇌하수체 LH 분비의 맥동성과 급등이 역시 약화 내지 지연된다. 노화와 관련된 GnRH-LH 신경내분비 활성의 결손은 시상하부 GnRH 뉴런의 활성을 조절하는 여러 자극(예, glutamate)들의 변화와 밀접하게 연관되어 있다. 많은 연구자들이 이러한 '시상하부 결손(hypothalamic defects)'이 생식 노화의 주된 요인임을 지지하지만, inhibin과 같은 난소 요인의 변화 역시 생식 노화의 유도와 관련된 것으로 보인다. 생식 노화를 연구함에 유용한 몇몇 설치류 모델이 있다; FSH 수용체 녹아웃(follitropin receptor knockout; FORKO) 생쥐 모델의 경우는 homozygous(null) 뿐만 아니라 heterozygous(haploinsufficient) 상태도 노화에 따른 난자/난포의 고갈을 나타낸다. Dioxin/aryl hydrocarbon 수용체 녹아웃 (AhRKO) 생쥐 모델도 유사한 상태를 유발할 수 있다. 생식 노화의 기작에 관한 연구는 삶의 질을 높이기 위한 수단들, 예를 들어 호르몬 보충요법(HRT)의 장단점을 평가하고 안전성을 제고하는데 도움이 될 것이다.