• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.152-152
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• 2017

Plants exposed to environmental stress for long durations often can adapt to stress conditions with improved tolerance. Moreover this acquired tolerance to stress can be retained even after reverting to destressed growth conditions, which is known to stress memory. In these adaptation and stress memory processes, epigenetic regulation, such as DNA methylation and histone modifications play a key role. Here, we showed that regenerated rice plants from embryogenic callus exposed to gradually increasing NaCl concentrations (up to 120 mM NaCl) acquired salt tolerance and their enhanced tolerance are inherited to subsequent generations. The rice plants (R0) regenerated from rice callus under saline conditions were transplanted into normal paddy field and R1 seeds were harvested. These R1 seeds displayed higher germination rate on MS medium containing 100mM NaCl than wild-type. The callus derived from R1 seeds showed better growth than control callus on high salinity medium. And the salt-adapted R1 plants exhibited higher chlorophyll contents and also higher $K^+/Na^+$ ratio than wild-type rice under saline conditions. The results indicated that rice plants successfully adapted to saline growth conditions during regeneration on high salt medium and moreover this acquired tolerance to salt stress was inherited subsequent generation.

• 한국식품조리과학회지
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• 제23권6호
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• pp.785-792
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• 2007

The aim of this study was to develop rice cookies based on 'Goami 2' rice, and made with sesame (white and black) and perilla seeds. Furthermore, we studied the physicochemical and sensory characteristics of the cookies as well as the alternative effects of the sesames or perilla seeds for butter, where we substituted the sesames or perilla for 30% of the total butter. The pH values of the cookie doughs made with the sesames or perilla seeds were significantly different (p<0.001), whereas the spread factors of the doughs were not significantly different. The Hunter color L-, a-, and b- values were significantly different for the cookies made with the added sesames or perilla seeds (p<0.001). The brittleness of the Goami 2 rice cookies (control) was significantly higher than that of the cookies made with the sesames or perilla (p<0.001). In a descriptive analysis performed by 8 trained panelists, the following sensory characteristics: yellow, black, sweet taste, nutty taste, nutty odor, oily flavor, and off flavor, were significantly different among the cookie samples. In a consumer test by 50 women students, the Goami 2 rice cookies were preferred in the following order: Goami 2 rice cookies with no additions > black sesame cookies > white sesame cookies > perilla cookies. In conclusion, some physicochemical and sensory characteristics of the cookies were different, in relation to being made with or without the sesame or perilla seeds. However, because of the small difference in acceptance among the cookies, the sesame and perilla rice cookies show strong potentials for consumer acceptance. Moreover, the black sesame cookies were more acceptable in terms of butter substitution for the cookie preparation.

• 현장농수산연구지
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• 제16권1호
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• pp.17-24
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• 2014

Growth Chamber에서 철분코팅 벼종자와 무처리 볍씨의 발아 및 입묘 특성을 구명하기 위하여 유묘의 입모율, 경시적 입묘양상의 변화, 초장, 엽수, 근장, 근수, 생체중의 특성을 조사결과를 요약하면 다음과 같다. 1. 입모율은 철분코팅 95%, 무처리 95%로 매우 높은 결과를 보였으며, 벼 출아소요일수는 출아가 13~14일 소요되는 것으로 나타났으며, 철분코팅볍씨가 무처리 볍씨보다 전반적으로 출아가 빠르게 나타났다. 2. 벼 유묘의 초장에서는 철분코팅볍씨 4.9cm, 무처리볍씨 3.4cm로, 엽수는 철분코팅볍씨 3.8개, 무처리볍씨 3.3개로 각각 나타났다. 3. 유묘의 뿌리 철분코팅볍씨 3.8cm로 무처리볍씨 0.4cm보다 9.5배 최장 뿌리 길이가 긴 것으로 나타났으나, 뿌리수는 철분코팅볍씨 6.8개, 무처리볍씨 5.5개로 비슷한 수준으로 나타났다. 4. 유묘의 생체중은 철분코팅볍씨 107mg, 무처리 볍씨 66mg로 각각 나타났다.

• 한국작물학회지
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• 제43권3호
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• pp.157-160
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• 1998

To find out the effects of humidification and hardening of rice (Oryza sativa L. cv. Ilpumbyeo) seeds on the germination, both normal and artificially aged seeds with 60% germination rate were humidified at 40, 60, and 80% RHs and $25^{\circ}C$ for five weeks or hydrated for 6, 12, 18, and 24 hours at $25^{\circ}C$ from one to five cycles for hardening. Relative humidity and duration of humidification did not affect the germination rate of normal seeds, while reduced the time to get 50% germination rate ($T_{50}$) by 1.0～1.6 days compared to that of normal seeds at $25^{\circ}C$. Aged seeds humidified at 40 and 60% RHs did not affect the germination rate and $T_{50}$ regardless of relative humidity and duration of treatment, while at 80% RH, the germination rate decreased and $T_{50}$ increased significantly with the duration of humidification. Hardening of normal seeds reduced $T_{50}$ by 0.7～1.1 days without changes in the germination rate. However, the germination rate and $T_{50}$ of aged seeds soaked in water for 6, 12, and 18 hours were similar regardless of soaking/drying cycles, while hardening of artificially aged seeds with 1～4 cycles in 24 hours soaking increased the germination rate by 11-16% and reduced $T_{50}$ by 1.4～2.0 days.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.244-244
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• 2017

Soil salinity due to accumulation of salts particularly sodium chloride affects agricultural lands and their vegetation. Generally, rice is a moderately sensitive plant with some cultivars with varying tolerance to salinity. Though there are physiological differences between salt-sensitive and salt-tolerant rice cultivars, both are still affected especially during high salinity and prolonged exposure. This also ultimately affects their indigenous bacterial endophytes particularly those that inhabit the rice seed endosphere. This study investigates the dynamic structure of seed bacterial endophytes of salt-sensitive and tolerant rice cultivars grown in different levels of soil salinity. Endophytic bacterial diversity was studied Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis. Results revealed a very interesting pattern of diversity and shifts in community structure of bacterial endophytes in the rice seeds. There is a general decrease in diversity for the salt-sensitive rice cultivar, IR29 as soil salinity increases. For the salt-tolerant cultivars, IC32 and IC37, diversity interestingly increased at moderate salinity then decreased at high soil salinity. The patterns of community structure is also strikingly different for the salt-sensitive and salt-tolerant rice cultivars. IR29 has a more even distribution of abundance, but under soil salinity, the community shifted where Curtobacterium, Pantoea, Flavobacterium and Microbacterium become the more dominant bacterial communities. For IC32 and IC37, the dominant bacterial groups under normal stress conditions were also the dominant bacterial groups during salt stress conditions. Their seed bacterial community is dominated by endophytes belonging to Microbacterium, Flavobacterium, Pantoea, Kosakonia and Enterobacter. Stenotrophomonas and Xanthomonas have not changed in terms of abundance under different salinity stress level in the salt-sensitive and salt-tolerant rice cultivars. This study showed that soil salinity greatly influenced the seed bacterial communities of rice seeds irrespective of their physiological tolerance to salinity.

• 한국작물학회지
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• 제55권1호
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• pp.76-82
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• 2010

자운영 지속재배시 토양에 매몰된 종자의 휴면 및 발아특성을 조사하여 토양속 종자 동태를 구명하기 위해 자운영종자와 꼬투리의 토양매몰 깊이 및 기간별 휴면성과 종자활력 변화를 조사하고 또한 자운영 지속재배 포장에서 낙수후 자운영 종자의 토양속 분포 개수, 발아율 및 종자활력기간을 조사한 결과는 다음과 같다. 1. 자운영 종자 회수율은 종자 매몰 한달후부터 활력이 잃기 시작하여 낙수전 9월까지 52~65%, 낙수후 10월에는 급격히 떨어져 종자와 꼬투리 모두 30% 이하를 나타내었고, 꼬투리 매몰의 경우는 낙수전 9월까지 활력소실이 되지 않고 100%를 보였다. 2. 자운영 종자의 휴면은 수확당시는 95%로 높았으나 시간이 경과함에 따라 점차 파괴되어 9월에는 휴면율(경실율)이 종자는 12~22%, 꼬투리는 31~33%로 급격히 낮아졌고, 낙수후 10월에는 경실율이 종자는 2~8%, 꼬투리는 5~12%로 보다 더 낮아졌다. 3. 발아율은 토양 매몰전 종자는 4%로 낮았으나 토양에 120일간 매몰후 종자 및 꼬투리속 종자 발아율은 휴면이 타파되어 각각 21%~31%, 49~51%로 증가하였다. 4. 자운영 지속포장에서 9월 낙수후 토양속 잔존 자운영 종자는 로터리 부분경운이앙은 경운이앙보다 2.3~2.6배가 많았고, 자운영 종자분포 깊이는 로터리 경운은 0~15 cm 깊이에 고르게 분포되었으나 부분경운 이앙은 대부분의 종자가 5cm 이하 깊이에 분포하고 있었다. 5. 자운영 종자의 출아기간을 기준으로 한 토양 속에서 활력유지기간은 매몰 후 2년정도 인 것으로 조사되었다.

• 한국작물학회지
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• 제63권3호
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• pp.219-228
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• 2018

Weedy rice (Oryza sativa) is a semi-wild rice grown in paddy fields and is more adapted to the environment than cultivated rice. Therefore, it is expected that the seeds of weedy rice might contain some chemicals related to its environmental adaptation, such as antioxidants, which may be useful for nutritional and medicinal purposes. This study was carried out to investigate the antioxidant activities of weedy rice compared to a Korean-bred rice cultivar, Sindongjin (SDJ), and to screen lines that show higher antioxidant activity in 199 accessions of weedy rice germplasm collected in Korea. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity ranged from 31.0 to 91.7%, with an average of 82.5%, and 19 accessions that showed more than 91% antioxidant activity were selected. The 19 accessions were re-screened against non-germinated brown rice (BR) and germinated brown rice (GBR) using four assays, total phenol determination, DPPH radical scavenging, ABTS radical scavenging, and reducing power activities. The results showed that accession 'WD3' had the highest antioxidant capacity in both BR and GBR, suggesting that WD3 is a promising potential source of antioxidants and could be developed as a potentially functional substance material.

• 한국작물학회지
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• 제41권2호
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• pp.223-229
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• 1996

미맥 작부체계에서 보리수확과 동시 벼 이앙작업에 따른 노동력의 경합과 우리나라 기상 여건상 소득이 낮아 답리작으로 맥류재배를 회피하는 실정이다. 이에 생력화 방안으로 보리입모중 상태에서 시기별로 공시품종인 간척벼를 인력산파하여 파종시기와 파종량 및 파종당시의 종자 최아 유무에 따른 입모수 및 수량구성요소와 수량을 검토하였던 바, 그 결과를 요약하면 다음과 같다. 1. 입모수 및 입모율은 보리 수확당일 파종에서 가장 많았고, 파종량간에는 10kg /10a 수준에서, 그리고 건종자보다는 최아종자가 입모수가 많았으며 경향은 분얼기의 경수 및 수수에서도 같은 경향을 보였다. 2. 출수기는 맥후작 어린모기계이앙이 8월 20일이었는데, 수확 10 일 전 파종은 2일, 5일 전 파종은 3일 그리고 수확 당일 입모중 파종에서는 4일이 각각 지연되었다. 3. 보리 입모중 산파의 간장은 기계이앙보다 다소 짧았으나, 파종기 및 파종량간에는 차이가 없었다. 4. 도복정도는 수확 10일전과 5일전의 입모중 파종에서는 도복이 일어나지 않았으나, 수확당일 입모중 파종에서는 파종량이 많은. 8∼10kg/10a 수준에서 1∼3정도의 경미한 도복이 일어났다. 5. 수량은 맥후작 어린모기계이앙과 비교하여 보리입모중 파종은 파종시기가 이를수록 수량이 낮았고, 파종량이 많을수록 수량은 높았으며, 수확당일 입모중 파종에서 6kg/10a 수준이 3%, 8∼10kg/10a수준이 6% 정도 증수경향을 보였다.

• Journal of Biosystems Engineering
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• 제28권5호
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• pp.411-420
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• 2003

Physical and cultural properties were investigated on the rice-seed pellets made by the pelleting machine(Yu, 2003) as the changes with mixing ratios of soil to rice seed of 6 : 1, 7 : 1, and 8 : I, and rotating speeds of forming rolls of 7, 10, and 13 rpm. Average weight, average diameter, and average sphericity of the pellets were 1.70 g, 12.0 ㎜, and 99.1 ％, respectively. Average number of seeds per pellet was more than 3, and almost all pellets had more than 3 seeds in the cases of mixing ratios of 6 : 1, and 7 : 1 at the forming rolls' speed of 7 rpm. Gradual drying was needed because rapid drying caused cracks on surface of the pellets. Compression strength of the pellets dried in shady room was in the range of 132 ∼ 152 N, which was enough for handling. Comparing with the previous pellets(Park, 2002), average number of seeds per pellet, ratio of pellets including more than 3 seeds, and compression strength increased due to the effects of pressure feed of pellet materials, and improvements of the forming rolls. Emergence ratio of the pellets made at the mixing ratio of 6 : 1 and the forming rolls' speed of 7 rpm, was 100 % on dry paddy and was 97 % on flooded paddy surface. Good growth characteristics, and yield except number of seedling stand and ratio of missing plant were shown in planting of the pellets made at the mixing ratio of 7 : 1 and the forming rolls' speed of 7 rpm on flooded paddy surface field. Considering the cultural results, the mixing ratio of 6 : 1, and the forming rolls' speed of 7 rpm seems to be optimum operating condition for the improved pelleting machine.

• 한국작물학회지
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• 제10권
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• pp.51-56
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• 1971

청도군이서면과 김제군죽산면에서 발생된 수도심고선충을 분리하여 그 병실과 병원선충의 형태를 조사하고 그 전염경로와 온탕처리방제에 관한 약간의 실험을 실시하였는데 그 지과를 요약하면 다음과 같다. 1. 발병되었던 포장에서는 매년발병된다. 즉 포장전염을 한다. 2. 발병되었던 벼 종자는 그 종자를 통해서 전염한다. 3. 관개수를 통해서 전염한다. 4. 토양중에서 선충이 생존하고 있다가 토양의 이동으로 선충이 전염된다. 5. 선충은 이삭과 뿌리에 많이 분포되어 있지만 성숙식물전체부분에 널리 분포되여 있다가 그것을 통해서 전염된다. 6. 5$0^{\circ}C$, 55$^{\circ}C$, 6$0^{\circ}C$의 온탕에서 5분간격으로 60분까지 종자처리를 하여 살선충효과와 발아와의 관계를 조사하였던 바 5$0^{\circ}C$에서는 60분간 처리하여도 효과가 없고 55$^{\circ}C$에서는 15~20분간, 6$0^{\circ}C$에서는 10~15분간만 처리하여도 효과가 있음을 알았다.