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Development of a Functional Chungkookjang (Soybean Paste Fermented for 2-4 Days) with Anti-AGS Human Gastric Cancer Cell Properties

  • Park, Kun-Young;Jung, Keun-Ok;Kwon, Eun-Young
    • Preventive Nutrition and Food Science
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    • v.8 no.1
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    • pp.54-60
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    • 2003
  • To develop a functional chungkookjang; the anticancer effects of chungkookjangs prepared with different varieties of soybeans, starters, fermentation periods and seasoning additive ratios; were studied against AGS human gastric adenocarcinoma cells using the MTT assay, at different stages chungkookjang processing. The chungkookjang samples exerted different antiproliferative effects according to the variety of soybeans used. The chungkookjangs manufactured with soybean var. manrikong exhibited the highest cytotoxicity against AGS human cancer cells. The chungkookjangs fermented with rice straw and B. licheniformis strongly inhibited the growth of the AGS human cancer cells. All fermented chungkookjangs had a strong inhibitory effect on the growth of the cancer cells; however, the non-fermented soybean (chungkookjang) showed a low inhibition rate. The fermented chungkookjangs mixed with red pepper powder (RPP) and garlic exhibited strong antiproliferative effect against the cancer cells, and chungkookjang prepaved with 1.1 % RPP and 1.1 % garlic showed the highest cytotoxicity against the cancer cells. The functional chungkookjang fermented with soybean variety of manrikong and B. licheniformis for 3 days at 4$0^{\circ}C$ and then mixed with 7.9% salt, 1.l% RPP and 1.1% garlic, exhibited a higher antiproliferative effect than the chungkookjangs prepared by traditional or modified methods, according to the MTT assay. The functional chungkookjang exhibited a similar anticancer effect to the traditional doenjang. These results indicate that the fermentation period and the ratio of seasoning additives, as well as the variety of soybeans and starter cultures may affect the degree of the anticancer effect of chungkookjang.

Prospect of Soybean Production, Consumption and Supply in Korea (콩 생산 수급전망과 대책)

  • Kim Seok Dong;Park Keum Yong
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1998.10a
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    • pp.249-265
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    • 1998
  • The yearly consumption of soybean ranged from 1.5 to 1.7 million tons in Korea during 1995-1997 with an increasing trend of annual consumption by 60,000 tons. Gross consumption of soybean was 1.74 million tons in 1997: 1.292 million tons for feed, 0.433 million tons for food and its processing, and 15,000 tons for seed and other uses. Particularly, Korea totally depends upon imports of soybean for feed and oil. Only about $40{\%}$ of soybeans used for food and its processing are supplied through domestic production. Korean markets will be open to foreign agricultural products except for rice in 2004 when the Uruguay Round treaty is completed. According to the Korean Rural Economics Institutes reports, soybean consumption in 2004 is expected to be 1.87 million tons that is higher than that in 1997 by 0.13 million tons. In order to meet the need for soybean, Korean government planned to supply from 0.17 million tons of domestic production plus 1.7 million tons of imports, and also planned to raise the self-supply rate of $9.1{\%}$ in 2004 from $8.6{\%}$ in 1997. According to the USDA reports on international soybean production and consumption, its production is expected to be 150 million tons over the world and the international market prices for soybean will be unstable in 2004. Based on these reports, international soybean trade capacity will be 36 million tons in 2004 that is lower than 39 million tons (accounting for $25{\%}$ of gross production) in 1597. Also, a term-end stock in 2004 is estimated to be 9.6 million tons that is low as compared to 18.6 million tons In 1997, In coping with domestic and international soybean production, consumption and supply, and further possible food crisis, national policies and continuous efforts are necessarily required to promote domestic production and to reduce imports of soybean.

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Reuse of Exhaust Heat and Improvement in Fuel Efficiency of Grain Dryer (곡물(穀物) 건조기(乾燥機)의 배기열(排気熱) 재이용(再利用) 및 열효율(熱効率) 개선(改善)에 관(關)한 연구(硏究))

  • Keum, Dong Hyuk;Lee, Yong Kook;Lee, Kyou Seung;Han, Jong Ho
    • Journal of Biosystems Engineering
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    • v.9 no.2
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    • pp.65-73
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    • 1984
  • While most of researches on the performance of high temperature grain dryer have dealt mainly with improving dryer capacity and drying speed during the last twenty years, energy efficiency, in fact, has not been emphasized. Current fuel supplies and energy cost have shifted the emphasis to reducing the energy consumption for grain drying while maintaining dryer capacity and grain quality. Since the energy input for drying is relatively large, the recovery and reuse of at least part of the exhaust energy can significantly reduce the total energy consumption in existing drying systems. Unilization of exhaust heat in grain dryer either through direct recycling or by a thermal coupling in heat exchanger have been subject of a number of investigators. However, very seldom research in Korea has been done in this area. Three drying tests(non-recycling, 0.22 recycle ratio, and 0.76 recycle ratio)were performed to investigate the thermal efficiency and heat loss factors of continuous flow type dryer, and to analyze the effect of recycle ratio (weight of exhaust air recycled/total weight of input air) on the energy requriements for rough rice drying. The test results showed that when the exhaust air was not recycled, the energy lost from furnace was 15.3 percent of input fuel energy, and latent and sensible heat of exhaust air were 61.4 percent and 11.2 percent respectively. The heat which was required in raising grain temperature and stored in dryer was relatively small. As the recycle ratio of exhaust air was increased, the drying rate was suddenly decreased, and thermal efficiency of the kerosene burner was also decreased. Drying test with 0.76 recycle ratio resulted in 12.4% increase in fuel consumption, and 38.4% increase in electric power consumption as compared to the non-recycled drying test. Drying test of 0.22 recycle ratio resulted in 6.8% saving in total energy consumption, 8.0% reduction in fuel consumption, and 2.5% increase in electric power consumption as compared to the non-recycled drying test.

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Functional Analysis of the High Affinity Phosphate Transporter Genes Derived from Oryza sativa in Arabidopsis thaliana. (애기장대에서의 벼 유래의 고친화성 인산 운반체 유전자들의 기능 분석)

  • Seo, Hyoun-Mi;Jung, Yun-Hui;Kim, Yun-Hye;Kwon, Tack-Min;Jeong, Soon-Jae;Yi, Young-Byung;Kim, Doh-Hoon;Nam, Jae-Sung
    • Journal of Life Science
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    • v.18 no.4
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    • pp.488-493
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    • 2008
  • Phosphate, a favorable phosphorous form for plant, is one of major nutrient elements for growth and development in plants. Plants exhibit various physiological and biochemical responses in reaction to phosphate starvation in order to maintain phosphate homeostasis. Of them, expression of high affinity phosphate transporter gene family and efficient uptake of phosphate via them is a major physiological process for adaption to phosphate deficient environment. Although the various genetic resources of high affinity phosphate transporter are identified recently, little is known about their functions in plant that is prerequisite information before applying to crop plants to generate valuable transgenic plants. We demonstrated that Arabidopsis transgenic plants over-expressing two different high affinity phosphate transporter gens, OsPT1 and OsPT7, derived from rice, exhibit better growth responses compared with wild-type under phosphate starvation condition. Specially, OsPT7 gene has proven to be more effective to generate Arabidopsis transgenic plant tolerant to phosphate deficiency than OsPT1. Furthermore, the expression level of AtPT1 gene that is one of reporter genes specifically induced by phosphate starvation was significantly low compared with wild-type during phosphate starvation. Taken together, these results collectively suggest that over expression of OsPTl and OsPT7 genes derived from monocotyledonous plant function efficiently in the dicotyledonous plant, relieving stress response caused by phosphate starvation and leading to better growth rate.

Change in Physicochemical and Storage Characteristics of Jeungpyun by Addition of Pectin and Alginate powder (펙틴, 알긴산가루를 첨가한 증편의 이화학적 특성과 저장 중 변화)

  • Park, Mie-Ja
    • Korean journal of food and cookery science
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    • v.21 no.6 s.90
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    • pp.782-793
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    • 2005
  • This study investigated the effect of $2\%$ addition of pectin and alginate on the Physicochemical and retrogradation Properties of Jeungpyun, a Korean traditional fermented rice cake. The volume of batters with alginate and Pectin was significantly larger than that of control. Jeungpyun samples with Pectin and alginate had a larger volume with uniform and smaller cell size. All samples showed largest foaming capability after second fermentation. Foaming capability of the control ($0\%$ addition of pectin and alginate) was significantly larger than that of the treated samples. The amount of reducing sugar tended to increase during fermentation but decreased after steaming, which was due to the increase in hydrolysis of starch. On the contrary, the content of free sugar was slightly decreased during fermentation but slightly increased after steaming. The control contained the largest amount of free sugar after steaming. The microstructure of starch particles after fermentation showed completely dispersed starch granules with air bubbles. After steaming, the structure was sponge-like in all samples. Samples with added alginate and pectin had significantly higher water binding capacity than those of the control. All samples showed noticeably increased solubility and swelling power at $70^{circ}C$ with the control being significantly lower than the treated samples. Retrogradation was measured with $\alpha$-amylase and the retrogradation process of the sample with added alginate and pectin proceeded slower than that of the control. The relative crystallinity was observed through X-ray diffraction method and samples with added alginate and pectin had smaller crystallinity and delayed retrogradation compared to the control. Thus, Jeungpyun with the addition of alginate and Pectin demonstrated improved functionality and dietary fiber addition effect. The storage period of was extended as the retrogradation rate was delayed by the addition of dietary fibers.

Supplement Dose and Health-Related Life Style of Vitamin-Mineral Supplement User among Korean Middle-Aged (중년기의 비타민ㆍ무기질 보충제 사용량과 건강관련 생활습관 조사)

  • 김윤정;문주애;민혜선
    • Korean Journal of Community Nutrition
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    • v.9 no.3
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    • pp.303-314
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    • 2004
  • We studied daily micronutrient intake from vitamin-mineral supplements, health-related life style, clinical case of diseases and food frequency of the Korean middle-aged (40-59 yr, n = 404) to compare the characteristics of non-user (n = 270) and user (n = 134) of vitamin-mineral supplements. Rate of supplement use of the middle-aged was 33.2% and there was significant difference in education level (p = 0.0084) and family income (p = 0.0476) of user and nonuser. Smoking habit (p = 0.0844) and drinking frequency (p = 0.0606) tended to be lower in a supplement user than a non-user. The medical history of a case was significantly higher in users (67.9%) than in non-users (44.4%) (p = 0.001), which suggests that medical history is one of the important motivations of supplement use. Supplement users had the medical history of digestive disease (34.1%), anemia (11.0%) and hypertension (9.9%) in order. Vitamin C was the most frequently supplemented nutrient (81.3%) among vitamin-mineral supplement, and the next orders were vitamins E (73.1%), B$_2$(68.7%) and B$_{6}$ (60.4%). Mean intakes of vitamin B$_1$, iron, selenium, vitamin E, and vitamin C from supplement was 4,260%, 4,030%, 1,660% and 1,330% of RDA, respectively. The supplement users tended to consume most food items including milk & milk products (p < 0.01), rice (p < 0.01), grains (p < 0.05) and cookies (p < 0.01) less frequently than non-users. Conclusively, nutrient intake of vitamin B$_1$, iron, selenium, vitamin E, and vitamin C from supplement was excessively high compared to RDA. We suggest that the toxic effect of excessive supplementation should he informed to supplement user and nutritional education should be focused on the optimal supplement dose.e.

Effect of Alcohol Content on the Consumer Acceptance and Sensory Characteristics of Makgeolli with Chinese Matrimony Vine (알코올 함량에 따른 구기자 막걸리의 소비자 기호도 및 묘사 특성)

  • Kwak, Han Sub;Kim, Inyong;Yin, Maoyuan;Lee, Yunbum;Kim, Mi Jeong;Lee, Youngseung;Kim, Misook;Jeong, Yoonhwa
    • The Korean Journal of Food And Nutrition
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    • v.30 no.4
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    • pp.719-727
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    • 2017
  • The objective of this study was to investigate the effect of alcohol content in Makgeolli made with Chinese matrimony vine (M-CMV) on the sensory profile and consumer acceptability. The M-CMVs were prepared with 6, 7, 8, and 9% alcohol content. Descriptive analysis of M-CMV was performed with six trained panelists. Thirteen attributes were generated and their intensities were alcohol content dependent. The consumer acceptance test was conducted with 57 consumers. M-CMV samples with 7% alcohol had the highest acceptance rate (5.8) followed by 6% M-CMV (5.6). Commercial rice Makgeolli (CRM) had the lowest consumer acceptance. Consumers were divided into two groups by clustering analysis. The majority of consumers (n=38) preferred M-CMV and did not like the commercial sample. Only 19 consumers indicated high acceptance ratings for CRM. However, these consumers also preferred 6 and 7% M-CMV. Partial least-squares regression analysis revealed moderate attribute intensities were related to greater consumer acceptability. The optimal alcohol content for the greatest consumer acceptance predicted by linear regression was 6.7%.

The Effect of Mild-Acid Treated Waxy Starches on the Yield of Resistant Starch (약산 처리가 찰전분의 저항전분수율에 미치는 영향)

  • 이신경;홍윤호;신말식
    • Korean journal of food and cookery science
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    • v.15 no.4
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    • pp.418-425
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    • 1999
  • The characteristics and yield of resistnat starch (RS) in waxy starches which were treated with 0.1N HCl were investigated. Hydrolysis rate of waxy starches were 0.32-0.88%. The yields of RS in waxy maize and waxy barley starches increased with acid treatment time up to 12hr and then decreased. The yield of RS in waxy rice starches increased with acid treatment up to 8hr and then decreased. The hydrolysis and yield of RS were different depending upon the varieties of starch source. Autoclaved normal maize starch showed a gel-like structure, but autoclaved normal maize starch showed crystal particle on the surface by SEM. The shape of autoclaved resistant starch was not different from acid treated ones. X-ray diffraction patterns of native and acid treated starches were all A-types. X-ray diffraction patterns of autoclaved waxy maize starches showed amorphous structures, however, those of RS showed all completely crystalline structure.

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Study on the Cold Storage Condition of Hypoaspis aculeifer(Canestrini)(Acari: Gamasida) and Tyrophagus putrescentiae(Schrank)(Acari: Acaridae) (총채가시응애(Hypoaspis aculeifer)와 긴털가루응애(Tyrophagus putrescentiae)의 저온저장 조건 구명)

  • Ham, Eun Hye;Choi, Young Cheol;Lee, Jun Seok;Park, Jong Kyun
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.109-111
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    • 2012
  • The success of biological control depends upon the standard setting and effective mass rearing of natural enemies. In this work, we have investigated cold storage condition of Hypoaspis aculeifer(Canestrini) and Tyrophagus putrescentiae(Schrank). which has been stored at 8, $12^{\circ}C$, RH $70{\pm}5%$, in dark condition. An appropriate temperature of cold storage was $12^{\circ}C$ with wheat bran(50%) and rice bran(50%) for T. putrescentiae. 7o% of T. putrescentiae and H. aculeifer could survive for 28 days and 70 days at $12^{\circ}C$. After storing at $12^{\circ}C$ with prey mite for 28 days, 56 days and 84 days, their reproduction rate showed similar resultes.

Regeneration of plants from alginate-encapsulated axenic nodal segments of Paederia foetida L. - A medicinally important and vulnerable plant species

  • Behera, Biswaranjan;Behera, Shashikanta;Shasmita, Shasmita;Mohapatra, Debasish;Barik, Durga Prasad;Naik, Soumendra Kumar
    • Journal of Plant Biotechnology
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    • v.48 no.4
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    • pp.255-263
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    • 2021
  • Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl2 (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 ℃; thus, they could be useful for transfer and exchange of P. foetida germplasm.