• Research in Plant Disease
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• v.12 no.1
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• pp.25-27
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• 2006

Rice dwarf phytoreovirus (RDV), a member of the family Reoviridae has a genome composed of 12 segmented dsRNAs designated as 51 to 512 with an increasing order of mobility in polyacrylamide gel electrophoresis (PAGE). RDV encode 12 structural and non-structural proteins, $P1{\sim}P12$ which are encoded by the $S1{\sim}S12$ segments of the dsRNA genome, respectively. In this experiment, we confirmed in situ localization of RDV particles and P12 in cytoplasm of infected rice plant. We observed specific reaction of the gold particles using virus particle and P12 protein specific antiserum with protein A-gold immunolabelling in electron microscope. It was observed that gold particles specifically react to virus particles in cytoplasm in case using the antiserum for virus particles. In the case of antiserum for P12 protein, gold particles sporadically existing on cytoplasm without existing in organelle of cytoplasm specifically. As this result, RDV P12 protein encoded by S12 located in cytoplasm.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.155-157
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• 2004

We isolated Rice dwarf virus (RDV) from infected plants in rice fields (Korea, Japan, China, the Philippines and Nepal) and analyzed their genomic dsRNAs by polyacrylamide gel eletrophoresis. The genomic dsRNAs of the isolates showed distinct electrophoretic mobility profiles. The S12 coding to nonstructural protein of Korean isolate (RDV-Kr) was further analyzed by sequencing. The S12 of RDV-Kr was 1,066bp long and coded for a protein composed of 312 amino acids including three open reading frames of P12, P120Pa and P120Pb. The sequence identities were 96% and 98.6% with Japanese isolates (H, AN), 94.7% with Nepalese isolate (NEL), 94% with Chinese isolate (CK) and the Philippines isolate (P).

• Korean journal of applied entomology
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• v.16 no.1 s.30
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• pp.65-67
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• 1977

Yield losses from rice dwarf virus infection are significant in Korea. Rice dwarf virus(RDV) was purified and RDV-antiserum was produced. The purified virus, mixed with an adjuvant(1:1) was injected every 10 to 14 days into rabbits. Three injections .were sufficient to produce an antiserum of 1/4,096 titer. The produced antisera will be used to facilitate the detection and identification of RDV in rice plants and in the RDV leafhopper vectors.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.150.3-151
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• 2003

To analyze the genome of Cucumber mosaic virus(CMV) in pepper, we developed a new extraction method for double-stranded RNA(dsRNA). To isolate the dsRNA, 0.1g of pepper leaves homogenized with 1ml of 5${\times}$EXB extraction buffer[0.5M glycin, 0.5M NaCl, 5mM EDTA(pH9.0/NaOH), 10％ Sodium N-lauryl salcosinate(NLS), 10％ Sodium dodecylsulfate(SDS)] and purified with the 1/4 volume of phenol： chloroform： isoamylalcohol(25：24：1). dsRNAs from the aqueous phase was precipitated with isopropanol. This procedure was able to detect a minimal amount of dsRNA from CMV infected plant tissue and to distinguish different CMV satellite RNAs by polyacrylamide gel electrophoresis(PAGE). Moreover, this method can be applied CMV infected in pepper or Rice dwarf virus (RDV) infected rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.129-133
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• 2007

To breed virus resistant rice variety, developing an efficient screening method is the most important. Two screening methods such as field screening and tray screening method have been used, but the efficiency of the field screening method is too low because of environment factors and the that of the tray screening method is good but screening capability is limited with only $200{\sim}300$ lines per year. To overcome those problems, mass screening method using screen house was developed. Barely as host plant of vector insect was grown in screen house in winter season. Then viruliferous insects are spread in the first spring of the initiation year and sustain them annually. Screening of virus resistance was tested two times in a year, the first screening was from April to June and the second from July to September. The virus infected rate of each susceptible varieties was increased to 92% for RSV and 100% for RDV from the second year. Also, this method can evaluate as many as $1,500{\sim}2,000$ pedigree lines in one time compared with the tray screening method. The result indicates that the mass screening method using screen house, which combines the advantages of the field and tray screening methods, is proven to be more efficient and reliable.