Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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In Situ Localization of Rice dwarf phytoreovirus P12 Protein in Infected Rice Plant

벼오갈병 바이러스 P12 단백질의 벼 감염세포 내 소재양식

  • Lee Bong-Choon (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Hong Yeon-Kyu (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Hong Sung-Jun (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Park Sung-Tae (Yeongnam Agricultural Research Institute, NICS, RDA)
  • 이봉춘 (농촌진흥청, 작물과학원, 영남농업연구소) ;
  • 홍연규 (농촌진흥청, 작물과학원, 영남농업연구소) ;
  • 홍성준 (농촌진흥청, 작물과학원, 영남농업연구소) ;
  • 박성태 (농촌진흥청, 작물과학원, 영남농업연구소)
  • Published : 2006.04.01
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Abstract

Rice dwarf phytoreovirus (RDV), a member of the family Reoviridae has a genome composed of 12 segmented dsRNAs designated as 51 to 512 with an increasing order of mobility in polyacrylamide gel electrophoresis (PAGE). RDV encode 12 structural and non-structural proteins, $P1{\sim}P12$ which are encoded by the $S1{\sim}S12$ segments of the dsRNA genome, respectively. In this experiment, we confirmed in situ localization of RDV particles and P12 in cytoplasm of infected rice plant. We observed specific reaction of the gold particles using virus particle and P12 protein specific antiserum with protein A-gold immunolabelling in electron microscope. It was observed that gold particles specifically react to virus particles in cytoplasm in case using the antiserum for virus particles. In the case of antiserum for P12 protein, gold particles sporadically existing on cytoplasm without existing in organelle of cytoplasm specifically. As this result, RDV P12 protein encoded by S12 located in cytoplasm.

벼오갈병바이러스(Rice dwarf virus, RDV)는 Phytoreovius 속에 속하며 게놈은 double stranded RNA의 12분절로 이루어져 있는데 전기영동상에서 크기가 큰 것부터 S1에서 S12로 명명한다. 본 실험에서는 RDV 입자 및 P12 단백질의 세포질 내에서의 소재양식을 확인하였다. 바이러스 입자 및 P12 단백질 특이적 항혈청에 protein A-gold 입자를 immunolabelling하여 전자현미경에서 gold입자의 존재양상을 관찰하였다. 바이러스 입자 특이적 항혈청을 사용한 경우에는 gold 입자가 세포질내의 바이러스 입자에 특이적으로 반응한 것이 관찰되었다. P12단백질 특이적 항혈청의 경우 gold 입자가 세포질내의 세포소기관에 특이적으로 존재하지 않고 세포질전체에 산재하여 존재하였다. 이 결과로서 RDV S12가 코드하는 단백질 P12가 세포질내에 존재함을 확인하였다.

Keywords

References

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