• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.329-329
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• 2017

The study was conducted to investigate the effects of different harvesting time on pasting properties of starch in three colored rices. Seven major parameters of starch pasting properties, peak viscosity (PKV), hot pasting viscosity (HPV), cool pasting viscosity (CPV), setback (CPV minus PKV), breakdown (PKV minus HPV), peak time, and pasting time were determined by Rapid Visco Analyzer. The peak viscosity, hot viscosity, cool viscosity and peak time were influenced by different harvesting times. Pasting time was delayed slightly with prolonged harvesting time in all rice cultivars. Pasting temperature in each rice cultivar differed from each harvesting time, and pasting temperature of the two rice cultivars, Hongjinju and Joseongheugchal, showed the highest at the 40 days after heading and then it decreased at the final harvesting time. With the delay of the harvesting time, peak viscosity, hot viscosity, cool viscosity, setback value and pasting temperature did not exhibit a regular trend depending on their genetic characteristics. Branch chain length distribution of amylopectin was demonstrated a distinct difference among these colored rices. In changes of amylopectin branch chain-length distribution, the amylopectin structure of Hongjinju rice cultivar as affected by different harvesting time, the shortest chain length of amylopectin in rice starch harvested at 20 days after heading was characterized by the significant increase in A chains with $DP{\geq}12$ and remarked decrease in long chains $37{\leq}DP$ compared to that of 30, 40, and 50 days after heading. In particular, when harvesting time is delayed the distribution percentage of short chain (A chains with $DP{\geq}12$) was increased except for the rice which harvested 20 days after heading. The similar results were also observed in Sintoheugmi rice cultivar like that of Hongjinju rice cultivar. Otherwise, distribution percentage of the shortest chain length of amylopectin in rice starch harvested at 20 days after heading was characterized by the significant decrease in A chains with $DP{\geq}12$ and remarked increase in B chains $13{\leq}DP{\geq}24$ compared to that of 30, 40, and 50 days after heading.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.73-73
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• 2017

To set an optimal harvest time of main 7 whole crop silage rice cultivars, Nokyang(Ny), Mogwoo(Mw), Mogyang(My), Jungmo1029(Jm1029), Jungmo1038(Jm1038), Nokwoo(Nw) and Yeongwoo(Yw), based on feed value, we analyzed their feed values such as percent crude protein(CP), crude fat(CF), crude ash(CA), neutral detergent fiber(NDF), acid detergent fiber(ADF) and lignin. It was evaluated every 10 days from heading to 40 days after heading(DAH). Total digestible nutrient(TDN) and relative feed value(RFV) was also calculated from ADF and NDF. As results, CP was generally in decline as increasing DAH in Ny, My, Jm1029, Jm1038 and Yw and decreased to 20 DAH in Mw, decreased after increasing to 30 days in Nw. The CP content of Ny was relatively the highest ranged from 5.3% to 10.1% and Mw the lowest 4.5% to 5.2%, compared to others. CF content tend to decrease as DAH increase in Ny, My and Nw and decreased after increasing to 30 days Jm1029, increased after decreasing to 20 days Jm1038 but was not shown distinct trend of increase or decrease in Mw. Especially, that of Yw's CF gradually increased as harvest time late but relative content the lowest from 1.46% to 2.29% among 7 cultivars. The CA content of Ny, My, Jm1029 and Jm1038 approximately decreased as DAH increased and that of Mw was similar to others after heading, Nw decreased after 10 days and Yw increased after flowering. In all 7 cultivars, NDF and ADF had a tendency to decrease as days accumulated, in particular, Yw was the lowest on 30 DAH and so the content of Yw's TDN the highest(71.5%), while the lowest(67.2%) in Nw. For lignin, particularly, Mw tend to be in decline as DAH increase and was the lowest ranged from 1.34% to 1.87%. ADF analyses allows for the evaluation of in vivo digestible dry matter(DDM) and energy availability and NDF analyses provides the best indication of dry matter intake(DMI). Ultimately, the two factors can be combined to derive RFV for forage. RFV in general increased as DAH increased in all cultivars and was in order, Yw>My>Ny> Jm1038>Mw>Jm1029>Nw on 30 DAH. Taken together when these results, despite a slight increase of TDN after 30 DAH except Yw, considering forage yield potential, digestibility of grains and gradual decrease of CP and CF, the yellow ripe stage, about 30 DAH, was appropriate to harvest whole crop rice for silage use.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.90-90
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• 2005

• Korean Journal of Plant Resources
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• v.29 no.6
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• pp.658-669
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• 2016

Rice blast, caused by a fungus Magnaporthe oryzae, is one of the most devastating diseases of rice worldwide. Analyzing the valuable genetic resources is important in making progress towards blast resistance. Molecular screening of major rice blast resistance (R) genes was determined in 2,509 accessions of rice germplasm from different geographic regions of Asia and Europe using PCR based markers which showed linkage to twelve major blast R genes, Pik-p, Pi39, Pit, Pik-m, Pi-d(t)2, Pii, Pib, Pik, Pita, Pita/Pita-2, Pi5, and Piz-t. Out of 2,509 accessions, only two accessions had maximum nine blast resistance genes followed by eighteen accessions each with eight R genes. The polygenic combination of three genes was possessed by maximum number of accessions (824), while among others 48 accessions possessed seven genes, 119 accessions had six genes, 267 accessions had five genes, 487 accessions had four genes, 646 accessions had two genes, and 98 accessions had single R gene. The Pik-p gene appeared to be omnipresent and was detected in all germplasm. Furthermore, principal component analysis (PCA) indicated that Pita, Pita/Pita-2, Pi-d(t)2, Pib and Pit were the major genes responsible for resistance in the germplasm. The present investigation revealed that a set of 68 elite germplasm accessions would have a competitive edge over the current resistance donors being utilized in the breeding programs. Overall, these results might be useful to identify and incorporate the resistance genes from germplasm into elite cultivars through marker assisted selection in rice breeding.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.318-321
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• 2011

Rice ageing describes the changes in physical and chemical properties of rice grains during storage that alter the eating quality of rice. The prevention of deterioration in palatability of rice during storage is important for stable supply. In this study, We conducted to select the variety with superior palatability after storage at room temperature. 'Yeonghojinmi' had much higher head rice ratio of milled rice than that of 'Ilmi', 'Junam'and 'Hwayeong'at similar moisture content of approximately 15%. Grossiness and stickiness were higher in 'Yeonghojinmi' than that of the others. Meanwhile, yellowness(b value) of cooked rice was lower in 'Yeonghojinmi' than that of the others. Of the four varieties, Yeonghojinmi was maintained good eating quality after storage in room temperature, which also contributed to differences in palatability, such as protein content, glossiness, stickiness and color of cooked rice.

• Plant Breeding and Biotechnology
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• v.2 no.3
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• pp.224-230
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• 2014

Amplified fragment length polymorphism (AFLP) is a molecular marker technique based on DNA and is extremely useful in detection of high polymorphism between closely related genotypes like Korean wheat cultivars. Six sequence characterized amplified regions (SCARs) have been developed from inter simple sequence repeat (ISSR) analysis which enabled the identification and differentiation of 13 Korean wheat cultivars from the other cultivars. We used six combinations of primer sets in our AFLP analysis for developing additional cultivar-specific markers in Korean wheat. Fifty-eight of the AFLP bands were isolated from EA-ACG/MA-CAC, EA-AGC/MA-CTG and EA-AGG/MA-CTA primer combinations. Of which 40 bands were selected to design SCAR primer pairs for Korean wheat cultivar identification. Three of 58 amplified primer pairs, KWSM006, KWSM007 and JkSP, enabled wheat cultivar identification. Consequently, 23 of 32 Korean wheat cultivars were classified by eight SCAR marker sets.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.7-7
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• 2017

Unfavorable weather and soil conditions reduce rice yield and land and water productivity, aggravating existing encounters of poverty and food insecurity. These conditions are foreseen to worsen with climate change and with the unceasing irrational human practices that progressively debilitate productivity despite global appeals for more food. Our understanding of plant responses to abiotic stresses is advancing and is complex, involving numerous critical processes - each controlled by several genetic factors. Knowledge of the physiological and molecular mechanisms involved in signaling, response and adaptation, and in some cases the genes involved, is advancing. Moreover, the genetic diversity being unveiled within cultivated rice and its wild relatives is providing ample resources for trait and gene discovery, and this is being scouted for rice improvement using modern genomics and molecular tools. Development of stress tolerant varieties is now being fast-tracked through the use of DNA markers and advanced breeding strategies. Large numbers of drought, submergence and salt tolerant varieties were commercialized over recent years in South and Southeast Asia and more recently in Africa. These varieties are making significant changes in less favorable areas, transforming lives of smallholder farmers - progress considered incredulous in the past. The stress tolerant varieties are providing assurance to farmers to invest in better management of their crops and the ability to adjust their cropping systems for even higher productivity and more income, sparking changes analogous to that of the first green revolution, which previously benefited only favorable irrigated and rainfed areas. New breeding tools using markers for multiple stresses made it possible to develop more resilient, higher yielding varieties to replace the aging and obsolete varieties still dominating these areas. Varieties with multiple stress tolerances are now becoming available, providing even better security for farmers and lessening their production risks even in areas affected by complex and overlapping stresses. The progress made in these less favorable areas triggered numerous favorable changes at the national and regional levels in several countries in Asia, including adjusting breeding and dissemination strategies to accelerate outreach and enabling changes at higher policy levels, creating a positive environment for faster progress. Exploiting the potential of these less productive areas for food production is inevitable, to meet the escalating global needs for more food and sustained production systems, at times when national resources are shrinking while demand for food is mounting. However, the success in these areas requires concerted efforts to make use of existing genetic resources for crop improvement and establishing effective evaluation networks, seed production systems, and seed delivery systems to ensure faster outreach and transformation.

• Molecules and Cells
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• v.21 no.2
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• pp.284-293
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• 2006

Even though Ac/Ds gene-tagging systems have been established in many higher plants, maize is the only major plant in which short-distance transposition of Ac/Ds has been utilized to probe gene function. This study was performed to evaluate the efficiency of obtaining new alleles and functional revertants from Ds insertion loci in rice. By analyzing 1,580 plants and the progeny of selected lines, the insertion sites and orientations of Ds elements within 16 new heritable alleles of three rice loci were identified and characterized. Intragenic transposition was detected in both directions from the original insertion sites. The closest interval was 35 bp. Three of the alleles had two Ds elements in cis configuration in the same transcription units. We also analyzed the excision footprints of intragenic and extragenic transpositions in Ds-inserted alleles at 5 loci. The 134 footprints obtained from different plants revealed predominant patterns. Ds excision at each locus left a predominant footprint at frequencies of 30-75%. Overall, 66% of the footprints were 7-bp additions. In addition, 16% of the excisions left 0-, 3-, 6-, and 9-bp additions with the potential of conserving reading frame.

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.04a
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• pp.63-64
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• 1998

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.11a
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• pp.157-157
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• 2020