• Title/Summary/Keyword: rice blast.

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Overexpression of a Rice Diacylglycerol Kinase Gene OsBIDK1 Enhances Disease Resistance in Transgenic Tobacco

  • Zhang, Weidong;Chen, Jie;Zhang, Huijuan;Song, Fengming
    • Molecules and Cells
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    • v.26 no.3
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    • pp.258-264
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    • 2008
  • A rice diacylglycerol kinase (DGK) gene, OsBIDK1, which encodes a 499-amino acid protein, was cloned and characterized. OsBIDK1 contains a conserved DGK domain, consisting of a diacylglycerol kinase catalytic subdomain and a diacylglycerol kinase accessory subdomain. Expression of OsBIDK1 in rice seedlings was induced by treatment with benzothiadiazole (BTH), a chemical activator of the plant defense response, and by infection with Magnaporthe grisea, causal agent of blast disease. In BTH-treated rice seedlings, expression of OsBIDK1 was induced earlier and at a higher level than in water-treated control seedlings after inoculation with M. grisea. Transgenic tobacco plants that constitutively express the OsBIDK1 gene were generated and disease resistance assays showed that overexpression of OsBIDK1 in transgenic tobacco plants resulted in enhanced resistance against infection by tobacco mosaic virus and Phytophthora parasitica var. nicotianae. These results suggest that OsBIDK1 may play a role in disease resistance responses.

The Properties of Concrete Compressive Strength used Rice Straw Ash (소성된 볏짚을 혼입한 콘크리트 압축강도 특성)

  • Kim, Young-Soo;Shin, Sang-Yeop;Jeong, Euy-Chang
    • Journal of the Regional Association of Architectural Institute of Korea
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    • v.21 no.5
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    • pp.117-124
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    • 2019
  • When manufacturing concrete, several mineral admixture is added to improve the basic physical property and durability and to make economical concrete. Such mineral admixture includes fly ash, granulated blast furnace slag, silica fume, etc., and not only the studies about mixing these mineral admixtures but also the studies for the development of new materials have been steadily in progress. Recently, some researchers have found, as a part of the development of new materials, the rice straw ash can also be used as a pozzolanic material for concrete considering similar chemical properties of rice straw ash to that of rice husk ash. But there has been insufficient amount of study about it. So, this study was to investigate the possibility as mineral admixture of agriculture by-product, by analyzing properties of concretes using rice straw ash with replacement ratio in comparison with other mineral admixture. In order to measure amount of SiO2 of rice straw ash, XRF(X-ray fluorescence) analysis was tested. For the measure pozzolanic reaction of rice straw ash, pH change and color change was tested according to curing day. Also to evaluate properties of concrete using rice straw ash, slump test, air contents test and compressive strength was tested.

Uniformity Among Magnaporthe grisea Isolates on Appressorium Formation by cDNA and Hydrophobicity of Contact Surface (cAMP와 표면 소수성에 의한 도열병균의 부착기 형성)

  • 이용환;최우봉
    • Korean Journal Plant Pathology
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    • v.10 no.4
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    • pp.254-260
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    • 1994
  • Magnaporthe grisea, a causal agent of blast, forms a specialized infection structure, an appressorium, to infect host. Hydrophobicity of contact surface and cAMP have been suggested as a primary environmental signal and a second messenger to trigger and mediate appressorium formation in this fungus, respectively. To generalize these factors in field isolates of M. girsea, twenty isolates originated from rice and other gramineous hosts were tested. Seventeen including rice and non-rice isolates formed appressoria on hydrophobic surface, but none of isolates formed appressoria on hydrophilic surface. Eighteen isolates formed appressoria on hydrophilic surface in the presence of IBMX, an inhibitor of phosphodiesterase, except two rice isolates. These results strongly support the hypothesis that appressorium formation by M. grisea is induced by hydrophobic hard surface and regulated by the endogenous level of cAMP in the cells. Understanding fungal development is not only of biological interest but provides new targets for novel disease control strategies.

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