• The Plant Pathology Journal
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• 제20권3호
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• pp.229-233
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• 2004

The bacterial leaf blight, which is caused by Xantho-monas oryzae pv. oryzae, is the most damaging and intractable disease of rice. To identify the genes involved in the virulence mechanism of transposon TnS complex, which possesses a linearized transposon and transposase, was successfully introduced into X. oryzae pv. oryzae by electroporation. The transposon mutants were selected and confirm the presence of transposition in X. oryzae pv. oryzae by the PCR amplification of transposon fragments and the Southern hybridization using these mutants. Furthermore, transposon insertion sites in the mutant bacterial chromosome were deter-mined by direct genomic DNA sequencing using transposon-specific primers with ABI 3100 Genetic Analyzer. Efficiency of transposition was influenced mostly by the competence status of X. oryzae pv. oryzae cells and the conditions of electroporation. These results indicated that the insertion mutagenesis strategy could be applied to define function of uncharacterized genes in X. oryzae pv. oryzae.

• 한국응용곤충학회지
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• 제22권2호
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• pp.74-83
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• 1983

Yield losses due to diseases and insect pests were mentioned and emphasized the efficiency of resistant cultivars in curving the yield losses and increasing chemical efficiency. Present status of resistance breeding for blast, bacterial leaf blight viruses, brown planthopper and white backed planthopper were introduced and the resistance sources for those are discussed. Breeding strategies for above items were presented. Specially for the blast resistance, discussions were made in some detail. With brief future prospects of resistance breeding in Korea, a suggestion was made for pathologists to make clear about whether the blast spores will be brought from mainland China as we see with Bph and Wbph or not.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1287-1292
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• 2013

Extracellular biogenic synthesis of silver nanoparticles with various shapes using the rice bacterial blight bacterium Xanthomonas oryzae pv. oryzae BXO8 is reported. The synthesized silver nanoparticles were characterized by UV-Vis spectroscopy, powder X-ray diffractometry (XRD), scanning electron microscopy, energy dispersive X-ray spectrometry, and high-resolution transmission electron microscopy (HR-TEM). Based on the evidence of HR-TEM, the synthesized particles were found to be spherical, with anisotropic structures such as triangles and rods, with an average size of 14.86 nm. The crystalline nature of silver nanoparticles was evident from the bright circular spots in the SAED pattern, clear lattice fringes in the high-resolution TEM images, and peaks in the XRD pattern. The FTIR spectrum showed that biomolecules containing amide and carboxylate groups are involved in the reduction and stabilization of the silver nanoparticles. Using such a biological method for the synthesis of silver nanoparticles is a simple, viable, cost-effective, and environmentally friendly process, which can be used in antimicrobial therapy.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1995년도 Proceedings of special lectures on Molecular Biological Approaches to Plant Disease National Agricultural Science and Technology Institute Suwon, Korea
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• pp.21-25
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• 1995

;Bacterial blight caused by Xanthomonas oryzae pv. Olyzae is one of the most important diseases of rice. Host resistance, which relies on single, dominant resistance genes, is the only reliable method to control the disease at present. Pathogenic variation of the bacteria has been shown to follow the deployment of resistance genes in commercial cultivars. Information on the factors and the mechanisms for genetic variation of this pathogen is limited. Further, we have no clear evidence of whether population variability is due to sexual recombination or to variation introduced by mutations or intragenic recombination in a clonally maintained population.(omitted)itted)

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 춘계학술대회
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• pp.175-175
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• 2018

• 한국응용곤충학회지
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• 제23권1호
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• pp.1-6
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• 1984

백엽고병에 대한 저항성품종으로 알려진 밀양 30호의 이병화원인을 구명하기 위하여 수원, 밀양, 해남산에 대한 최고분얼기, 출수기엽과 기본식물에 대한 각균군의 균주별 저항성 관계를 조사한 결과 가. $1979\~'81$년 전국에서 이병된 밀양 30호의 침해균군 비친화성으로 밝혀진 I, II균군의 균주가 다수분리되었다. 나. 산지 및 생육시기별 저항성 정도의 차는 인정할 수 없었으나 II 균군의 특수균주(병원성 분화형)에 대해 침해받았음이 원인이었다. 다. 밀양 30호의 기본식물에서도 동일 균군 균주에 의해 반응이 일치되므로 품종보다 균주의 병원성 분화에 따른 이병화임을 알 수 있었다.

• 농업과학연구
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• 제3권1호
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• pp.17-21
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• 1976

한국(韓國)에서 발병(發病) 되고있는 수도(水稻) 백엽고병(白葉枯病) 균계(菌系) 71-23에 대(對)한 WASE AIKOKU 3, ZENITH, TETEP 및 TKM 6품종등(品種等)의 저항성(抵抗性) 유전양식(遺傳樣式)을 구명(究明)하기 위(爲)하여 이들 저항성품종(抵抗性品種)을 이병성품종(罹病性品種)에 교배(交配)하여 모본(母本) 및 부본(父本)과 더불어 $F_1$ 및 $F_2$ 세대(世代)의 집단(集團)에서 검정(檢定)한 결과(結果)이들 품종(品種)의 저항성(抵抗性)은 1개(個)의 우성유전인자(優性遺傳因子)에 의(依)하여 지배(支配)되었다.

• Journal of Microbiology and Biotechnology
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• 제21권11호
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• pp.1147-1150
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• 2011

Xanthomonas oryzae causes rice bacterial blight, which has been reported as one of the most destructive diseases of rice. Metabolites were identified through cheonggukjang, a traditional Korean fermented soybean product fermented by the Bacillus spp., to control the bacteria. HPLC, MS, and UPLC-Q-TOF-MS analyses were performed to identify metabolites responsible for antimicrobial activity. In this analysis, the m/z values of 253.0498, 283.0600, 269.0455, 992.6287, and 1,006.6436 were identified as daidzein, glycitein, genistein, surfactin B, and surfactin A, respectively. The levels of surfactin B and surfactin A were found to be high at 24 h (4.35 ${\mu}g$/ml) and 36 h (3.43 ${\mu}g$/ml) of fermentation, respectively.

• Molecules and Cells
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• 제27권5호
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• pp.563-570
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• 2009

We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1609-1621
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• 2014

The plant pathogen Burkholderia gladioli, which has a broad host range that includes rice and onion, causes bacterial panicle blight and sheath rot. Based on the complete genome sequence of B. gladioli BSR3 isolated from infected rice sheaths, the genome of B. gladioli BSR3 contains the luxI/luxR family of genes. Members of this family encode N-acyl-homoserine lactone (AHL) quorum sensing (QS) signal synthase and the LuxR-family AHL signal receptor, which are similar to B. glumae BGR1. In B. glumae, QS has been shown to play pivotal roles in many bacterial behaviors. In this study, we compared the QS-dependent gene expression between B. gladioli BSR3 and a QS-defective B. gladioli BSR3 mutant in two different culture states (10 and 24 h after incubation, corresponding to an exponential phase and a stationary phase) using RNA sequencing (RNA-seq). RNA-seq analyses including gene ontology and pathway enrichment revealed that the B. gladioli BSR3 QS system regulates genes related to motility, toxin production, and oxalogenesis, which were previously reported in B. glumae. Moreover, the uncharacterized polyketide biosynthesis is activated by QS, which was not detected in B. glumae. Thus, we observed not only common QS-dependent genes between B. glumae BGR1 and B. gladioli BSR3, but also unique QS-dependent genes in B. gladioli BSR3.