• Journal of Agricultural Extension & Community Development
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• v.24 no.2
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• pp.99-115
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• 2017

The purpose of this study is to analyze potential adoption rates and reusing patterns of the new rice direct seeding technology. The model constructed and employed in this study is a system dynamics model of farmer adoption and reusing patterns for this new technology over time. The model incorporates a causal loop diagram that explains interactions among rice cultivation subsystems with feedback loops and further attempts to build a causal loop model with stock-flow diagram for computer simulation. As one example of how the model can be used to provide insight to rice farmers, simulations are run over varying levels on the cultivation process of rice. The major finding is to demonstrate the utility of system dynamics simulation methodology in aiding the rice wet direct seeding farmers' decision making.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.80.1-80
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• 2003

Several plant and microbial genes that could confer disease resistance in transgenic rice plants are being cloned and characterized. We are currently constructing transgenic rice lines that overexpress the gene products, such as a galactinol synthase, a defensin, and a bacterial ACC deaminase. Subtractive hybridization of a rice cDNA library constructed from the Xanthomonas oryzae-infected ice leaves resulted in isolation of many inducible cDNA clones including a elongation factor EF2, a oryzain alpha, a catalase, a aldehyde dehydrogenase, a S-adenosylmethionine synthetase, a caffeic acid O-methyltransferase, a glyceraldehyde-3-phosphate dehydrogenase, a light-regulated protein, nKY transcription factors, and a nucleotide diphosphate kinase. Some genes among those may be useful genetic sources for construction of disease resistant transgenic rice. Full lengths of the rice OsFIERG and a rice oryzain genomic clones were cloned, and serial deletion fragments of the promoter regions of these genes were fused with GUS reporter gene in pCAMBIA1201, respectively. Promoter activities of these constructs will be examined upon various stresses and Pathogen infections to obtain the pathogen specific inducible-promoter. This work was supported by a grant from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

• The Plant Pathology Journal
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• v.24 no.4
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• pp.375-383
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• 2008

It is well known that NtMEK2, a tobacco MAPK kinase, is the upstream kinase of both salicylic acid-induced protein kinase and wound-induced protein kinase. In addition, expression of $NtMEK2^{DD}$, a constitutively active mutant of NtMEK2, is known to induce multiple defense responses in tobacco. In this study, transgenic rice plants that contained an active or inactive mutant of NtMEK2 under the control of a steroid inducible promoter were generated and used to determine if a similar MAPK cascade is involved in disease resistance in rice. The expression of $NtMEK2^{DD}$ in transgenic rice plants resulted in HR-like cell death. The observed cell death was preceded by the activation of endogenous rice 48-kDa MBP kinase, which is also activated by Xanthomonas oryzae pv. oryzae, the bacterial blight pathogen of rice. In addition, prolonged activation of the MAPK induced the generation of hydrogen peroxide and up-regulated the expression of defense-related genes including the pathogenesis-related genes, peroxidases and glutathione S-transferases. These results demonstrate that NtMEK2 is functionally replaceable with rice MAPK kinase in inducing the activation of the downstream MAPK, which in turn induces multiple defense responses in rice.

• Applied Biological Chemistry
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• v.36 no.2
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• pp.111-114
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• 1993

The volatile components of cooked glutinous rice were investigated. Among more than 100 volatile components of fresh cooked rice, the predominant one was hexanal followed by pentanal, butanal, octanal, heptanal, noananal, acetone and toluene. These eight components comprised about 85% of the total volatiles, and the hexanal comprised about 86% total amount of the eight volatile components. the amount of the eight volatile components and hexanal in cooked glutinous rice were six and nine times more than that of cooked nonglutinous rice, respectively. The amount of volatile components in cooked glutinous rice stored for 72 hours at $70^{\circ}C$ was same with that of in nonglutinous rice immediately after cooking.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.4
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• pp.271-277
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• 2015

Anaerobic decomposition of organic material in flooded rice fields produces methane, which escapes to the atmosphere primarily by transport through the rice plants. The annual amount of $CH_4$ emitted from a given area of rice is a function of the number and duration of crops grown, water regimes before and during cultivation period, and organic and inorganic soil amendments. Soil type, temperature, and rice cultivar also affect $CH_4$ emissions. The field experiment was conducted for three years to develop methane emission factor for water regime before the cultivation period from the rice fields. It was treated with three different water regimes prior to rice cultivation, namely: non-flooded pre-season < 180 days, non-flooded pre-season > 180 days, flooded per-season in which the minimum flooding interval is set to 30 days. Methane emission increased with days after transplanting and soil redox potential (Eh) decreased rapidly after flooding during the rice cultivation. The average methane emission fluxes were $5.47kg\;CH_4\;ha^{-1}day^{-1}$in flooded pre-season > 30 days, 5.04 in non-flooded pre-season < 180 days and 4.62 in non-flooded pre-season > 180. Methane emission flux was highly correlated with soil temperature and soil Eh. Rice yields showed no difference among treatments with water regime before the cultivation period.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1459-1465
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• 2013

Two in vitro experiments were conducted to evaluate the effect of methylcellulose (MC) on i) bacterial detachment from rice straw as well as ii) inhibition of bacterial attachment and fiber digestibility. To evaluate the effect of MC on fibrolytic bacterial detachment (Exp 1), in vitro bacterial cultures with 0.1% (w/v) MC solution were compared with cultures without MC after 8 h incubation. The effect of MC on inhibition of bacterial attachment was determined by comparing with real-time PCR the populations of F. succinogenes, R. flavefaciens and R. albus established on rice straw pre-treated with 0.1% MC with those on untreated straw after incubation for 0, 6 and 12 h (Exp 2). The major fibrolytic bacterial attachment on rice straw showed significantly lower populations with either the addition of MC to the culture or pre-treated rice straw compared to controls (p<0.05). Also, the digestibility of rice straw with MC was significantly lower compared with control (p<0.05). The F. succinogenes population did not show detachment from rice straw, but showed an inhibition of attachment and proliferation on rice straw in accordance with a decrease of fiber digestion. The detachments of Ruminococcus species co-existed preventing the proliferations with subsequent reduction of fiber degradation by MC during the incubation. Their detachments were induced from stable colonization as well as the initial adhesion on rice straw by MC in in vitro ruminal fermentation. Furthermore, the detachment of R. albus was more sensitive to MC than was R. flavefaciens. These results showed the certain evidence that attachment of major fibrolytic bacteria had an effect on fiber digestion in the rumen, and each of fibrolytic bacteria, F. succinogenes, R. flavefaciens and R. albus had a specific mechanism of attachment and detachment to fiber.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.2
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• pp.167-176
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• 2011

Brown planthopper (BPH) is a serious insect pest of rice crop throughout rice growing countries, and yield loss due to its infection can be up to 60%. This study aimed to evaluate efficiency of molecular markers for screening BPH resistance accessions among 86 aromatic rice germplasm Eighty-six accessions of aromatic rice germplasm included two accessions of Tongil type (bred in Korea), 28 accessions of japonica type and 56 accessions of indica type. We applied eight STS markers (pBPH9, pBPH19, pBPH20, pBPH21, AJ09-b, RG457L, RG457B, and 7312.T4A) which were linked to four of BPH resistance genes, Bph1, Bph13(t), Bph10, and Bph18(t) respectively. One japonica type accession, 415XIr352, and six indica type accessions possessed one or four positive bands when tested with four STS markers linked to Bph1 gene. One indica type aromatic rice, Basmati9-93, showed the target bands linked to the Bph10 gene. The other accessions did not show same fragments as the respective resistant lines. Bph13(t) is the most widely introduced resistance gene and only one accession showed positive bands implying that this accession might harbor Bph10 and Bph18(t) genes. Three aromatic accessions, Domsiah, Khao Dawk Mali 105 and 415XIr352 showed gene pyramiding of Bph1 and Bph13(t). Two indica aromatic rice, Ds 20 and Basmati 9-93, possessed at least two BPH resistance genes, Bph1, Bph18(t) and Bph13(t), Bph18(t), respectively. These results indicates that aromatic rice germplasm have narrow diversities of BPR resistance genes.

• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.273-281
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• 2023

Ammonia (NH₃) emitted from the use of fertilizers during agricultural practice generates particulate matter and odors. The application of carbonized rice husk, an eco-friendly material, is one of the measures used to reduce NH₃. The objective of this study was to evaluate the effect of the application rate and pH of carbonized rice husk on NH₃ emissions and soil quality. An experiment to assess NH₃ emissions was performed in a glasshouse using a static chamber method. The pH of the carbonized rice husk was divided into acidic, neutral, and basic groups, and the carbonized rice husk application rates were 1, 3, and 5% of the soil weight. NH₃ emissions showed a sharp increase within three days after the inorganic fertilizer was applied. Subsequently, NH₃ emissions decreased rapidly after basal fertilization compared to primary and secondary top-dressing. When carbonized rice husks were applied to soil, NH₃ emissions decreased in all treatments, and neutral carbonized rice husk was the most effective in comparison with acidic and basic carbonized rice husk. The application rate of carbonized rice husk and NH₃ emissions showed a negative correlation, and the lowest emissions were found in units with a 5% application rate. Also, there was no statistically significant difference between NH₃ emissions according to the application rate of carbonized rice husk, and when carbonized rice husks were applied at a 5% rate, soil OM increased excessively. Therefore, it is recommended to apply only 1% neutral carbonized rice husk to most effectively reduce NH₃ emissions in the soil.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.545-553
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• 2012

In Asia, including Japan and Korea, rice paddies occupy the largest cultivated area in agricultural land. Rice paddies provide the habitats for many organisms including endemic species, sustaining high biodiversity. Insect natural enemies inhabiting rice paddies have an important function for rice production as agents of 'ecosystem services' because they play a major role in suppressing rice pests. The diversity and abundance of natural enemies can be a good index reflecting the 'healthiness' of agro-ecosystem services in rice paddies. The present study investigates whether insect natural enemies could be good biological indicators for general arthropod biodiversity and agricultural practice. First, the concept of ideal bio-indicators was summarized. The strategy to explore and select such bio-indicators was then proposed. Lastly, field survey was made to evaluate the abundance and biodiversity of natural enemies in Japanese rice paddies where chemical inputs, i.e., insecticide use, were different. The results showed that reduction of chemical inputs led to an increase in species richness or diversity of natural enemies including parasitoids and predators. Then, the data were analyzed to examine suitable indicator species to assess environmental soundness of agricultural practice and biodiversity in rice paddies. The density of several species of natural enemies did respond both to pesticide use and to general arthropod biodiversity. The analyses thus have indicated that natural enemies can be suitable as bio-indicators. Usefulness of indicator species in rice paddies is discussed in the context of ecologically sound agriculture.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.281-286
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• 2007

Gibberellins are growth hormones that play a pivotal role in the growth and development of plants. Present investigations were carried to check the effect of nitrogen(N) and silicon(Si) on bioactive $GA_1$ and its immediate precursor $GA_{20}$ at different growth stages of two rice cultivars with different maturity traits. It was observed that the endogenous bioactive $GA_1$ level gradually increased during vegetative stage and anthesis stage of both Junghwabyeo(early flowering cultivar) and Daesanbyeo(late flowering cultivar). However, the $GA_1$ and $GA_{20}$ content start decreasing during the seed filling stage in both rice cultivars, which indicated a possible relationship of bioactive $GA_1$ and floral development. Our results also confirmed that early 13-hydroxylation pathway was operated at all developmental stages of rice plant. Variation in the levels of the endogenous gibberellins in rice shoots were measured by GCMS-SIM using $^2H_2$-labeled gibberellins as internal standards. Combined application of N and Si enhanced growth parameters and reduced lodging index of both rice cultivars. It was thus concluded that the level of physiologically active $GA_1$ increased during vegetative and early reproductive stage, but starts declining at seed filling stage.