Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Methylcellulose on Fibrolytic Bacterial Detachment and In vitro Degradation of Rice Straw

  • Kim, Min Ji (Department of Agriculture Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Sung, Ha Guyn (Department of Animal Science and Technology, Sangji University) ;
  • Upadhaya, Santi Devi (Department of Agriculture Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Ha, Jong K. (Department of Agriculture Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Lee, Sung Sill (Division of Applied Life Science and IALS, Gyeongsang National University)
  • Received : 2013.04.16
  • Accepted : 2013.06.24
  • Published : 2013.10.01
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Abstract

Two in vitro experiments were conducted to evaluate the effect of methylcellulose (MC) on i) bacterial detachment from rice straw as well as ii) inhibition of bacterial attachment and fiber digestibility. To evaluate the effect of MC on fibrolytic bacterial detachment (Exp 1), in vitro bacterial cultures with 0.1% (w/v) MC solution were compared with cultures without MC after 8 h incubation. The effect of MC on inhibition of bacterial attachment was determined by comparing with real-time PCR the populations of F. succinogenes, R. flavefaciens and R. albus established on rice straw pre-treated with 0.1% MC with those on untreated straw after incubation for 0, 6 and 12 h (Exp 2). The major fibrolytic bacterial attachment on rice straw showed significantly lower populations with either the addition of MC to the culture or pre-treated rice straw compared to controls (p<0.05). Also, the digestibility of rice straw with MC was significantly lower compared with control (p<0.05). The F. succinogenes population did not show detachment from rice straw, but showed an inhibition of attachment and proliferation on rice straw in accordance with a decrease of fiber digestion. The detachments of Ruminococcus species co-existed preventing the proliferations with subsequent reduction of fiber degradation by MC during the incubation. Their detachments were induced from stable colonization as well as the initial adhesion on rice straw by MC in in vitro ruminal fermentation. Furthermore, the detachment of R. albus was more sensitive to MC than was R. flavefaciens. These results showed the certain evidence that attachment of major fibrolytic bacteria had an effect on fiber digestion in the rumen, and each of fibrolytic bacteria, F. succinogenes, R. flavefaciens and R. albus had a specific mechanism of attachment and detachment to fiber.

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References

  1. Akin, D. E. 1980. Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell walls. Appl. Environ. Microbial. 39:242-252.
  2. Bhat, S., R. J. Wallace, and E. R. Orskov. 1990. Adhesion of cellulolytic ruminal bacteria to barley straw. Appl. Environ. Microbiol. 56:2698-2703.
  3. Brock, F. M., C. W. Forsberg, and J. G. Buchanan-Smith. 1982. Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors. Appl. Environ. Microbiol. 44:561-569.
  4. Cheng, K. -J. and J. W. Costerton. 1980. Adhesive bacteria-Their role in the digestion of plant material, urea and epithelial cells. In: Digestive Physiology and Metabolism in Ruminants (Ed. Y. Ruckebusch and P. Thivend). MTP press Ltd., Lancaster, England. pp. 225-250.
  5. Cheng, K. -J., H. Kudo, S. H. Duncan, A. Mesbah, C. S. Stewart, A. Bernalier, G. Fonty, and J. W. Costerton. 1991. Prevention of fungal colonization and digestion of cellulose by the addition of methylcellulose. Can. J. Microbiol. 37:484-487. https://doi.org/10.1139/m91-081
  6. Craig, W. M., G. A. Broderick, and D. B. Ricker. 1987. Quantitation of microorganisms associated with the particulate phase of ruminal ingesta. J. Nutr. 117:56-62.
  7. Czerkawski, J. W. and K. -J. Cheng. 1988. Compartmentation in the rumen. In: The Rumen Microbial Ecosystem (Ed. P. N. Hobsn). Elsevier Science Publishing, New York, USA, pp. 361.
  8. Gong, J. and C. W. Forsberg. 1989. Factors affecting adhesion of Fibrobacter succinogenes S85 and adherence defective mutants to cellulose. Appl. Environ. Microbiol. 55:3039-3044.
  9. Hwang, I. H., C. H. Lee, S. W. Kim, H. G. Sung, S. Y. Lee, S. S. Lee, H. Hong, Y. -C. Kwan, and J. K. Ha. 2008. Effects of mixtures of Tween80 and cellulolytic enzymes on nutrient digestion and cellulolytic bacterial adhesion. Asian-Aust. J. Anim. Sci. 21:1604-1609. https://doi.org/10.5713/ajas.2008.80333
  10. Koike, S. and Y. Kobayshi. 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol. Lett. 204:361-366. https://doi.org/10.1111/j.1574-6968.2001.tb10911.x
  11. Kudo, H., K. -J. Cheng, and J. W. Costerton. 1987. Electron microscopic study of the methylcellulose-mediated detachment of cellulolytic rumen bacteria from cellulose fibers. Can. J. Microbiol. 33:267-272. https://doi.org/10.1139/m87-045
  12. Martínez, M. E., M. J. Ranilla, S. Ramos, M. L. Tejido, C. Saro, and D. C. Carro. 2009. Evaluation of procedures for detaching particle-associated microbes from forage and concentrate incubated in Rusitec ferrmenters: Efficiency of recovery and representativeness of microbial isolates. J. Anim. Sci. 87:2064-2072. https://doi.org/10.2527/jas.2008-1634
  13. McAllister, T. A., H. D. Bae, G. A. Jones, and K. -J. Cheng. 1994. Microbial attachment and feed digestion in the rumen. J. Anim. Sci. 72:3004-3018.
  14. McDougall, E. I. 1948. Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochem. J. 43:99-109.
  15. Michalet-Doreau, B., I. Fernandez, C. Peyron, L. Millet, and G. Fonty. 2001. Fibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents. Reprod. Nutr. Dev. 41:187-194. https://doi.org/10.1051/rnd:2001122
  16. Minato, H. and T. Suto. 1978. Technique for fractionation of bacteria in rumen microbial ecosystem. II. Attachment of bacteria isolated from bovine rumen to cellulose powder in vitro and elution of bacteria attached therefrom. J. Gen. Appl. Microbiol. 24:1-16. https://doi.org/10.2323/jgam.24.1
  17. Minato, H., M. Mitsumori, and K. -J. Cheng. 1993. Attachment of microorganisms to solid substrates in the rumen. Pages 139-145 in Proc. MIE Bioforum on Genetics, Biochemistry and Ecology of Lignocellulose Degradation. Institut Pasteur, Paris, France.
  18. Miron, J. and C. I. Forsberg. 1999. Characterization of cellulose binding proteins which are involved in adhesion mechanism of Fibrobacter intestinalis DR7. Appl. Microbiol. Biotechnol. 51:491-497. https://doi.org/10.1007/s002530051422
  19. Miron, J., D. Ben-Ghedalia, and M. Morrison. 2001. Invited Review: Adhesion mechanisms of rumen cellulolytic bacteria. J. Dairy Sci. 84:1294-1309. https://doi.org/10.3168/jds.S0022-0302(01)70159-2
  20. Miron, J., E. Morag, E. A. Bayer, R. Lamed, and D. Ben-Ghedakia. 1998. An adhesion defective mutant of Ruminococcus albus SY3 is impaired in its capability to degrade cellulose. J. Appl. Microbiol. 84:249-254. https://doi.org/10.1046/j.1365-2672.1998.00336.x
  21. Pell, A. N. and P. Schofield. 1993. Microbial adhesion and degradation of plant cell walls. Pages 397-423 in Forage Cell Wall Structure and Digestibility (Ed. R. D. Hatfield, H. G. Jung, J. Ralph, D. R. Buxton, D. R. Mertens, and P. J. Weimer). ASA-CSSA-SSSA, Madison, WI.
  22. Purdy, K. J., T. M. Embley, S. Takii, and D. B. Nedwell. 1996. Rapid extraction of DNA and rRNA from sediments by a novel hydroxyapatite spin-colum method. Appl. Environ. Microbiol. 62:3905-3970.
  23. Ranilla, M. J. and M. D. Carro. 2003. Diet and procedure used to detach particle-associated microbes from ruminal digesta influence chemical composition of microbes and estimation of microbial growth in Rusitec fermenters. J. Anim. Sci. 81:537-544.
  24. Rasmussen, M. A., B. A. White, and R. B. Hespell. 1989. Improved assay for quantitating adherence of ruminal bacteria to cellulose. Appl. Environ. Microbiol. 55:2089-2091
  25. Rasmussen, M. A., R. B. Hespell, B. A. White, and R. J. Bothast. 1988. Inhibitory effects of methylcellulose on cellulose degradation by Ruminococcus flavefaciens. Appl. Environ. Microbiol. 54:890-897.
  26. Reddy, S. K. K. and M. Morrison. 1998. Biochemical and molecular characterization of adherence-defective mutants of Ruminococcus albus strain 8. Page 132 in Proc. MIE Bioforum on Cellulose Degradation, Institut Pasteur, Paris, France.
  27. Roger, V. R., G. Fonty, S. Komisarczuk-Bony, and P. Gouet. 1990. Effects of physicochemical factors on the adhesion to cellulose avicel of the rumen bacteria Ruminococcus flavefaciens and Fibrobactor succinogenes subsp. succinogenes. Appl. Environ. Microbiol. 56:3081-3087.
  28. Statistical Analysis System Institute. 1996. SAS/STATTM User's Guide: Statistics, Version 7, 5th Edition. Vol. 3, Cary, NC.
  29. Stewart, C. S., S. H. Duncan, and H. J. Flint. 1990. he properties of forms of Ruminococcus flavefaciens which differ in their ability to degrade cotton cellulose. FEMS Microbiol. Lett. 72:47-50. https://doi.org/10.1111/j.1574-6968.1990.tb03859.x
  30. Sung, H. G., Y. Kobayashi, J. Chang, A. Ha, I. H. Hwang, and J. K. Ha. 2007. Low ruminal pH reduces dietary fiber digestion via reduced microbial attachment. Asian-Aust. J. Anim. Sci. 20:200-207.
  31. Trabalza-Marinucci, M., C. Poncet, E. Delval, and G. Fonty. 2006. Evaluation of techniques to detach particle-associated microorganisms from rumen contents. Anim. Feed Sci. Technol. 125:1-16. https://doi.org/10.1016/j.anifeedsci.2005.05.009
  32. Wang, Y., J. E. Ramirez-Bribiesca, L. J. Yanke, A. Tsang, and T. A. McAllister. 2012. Effect of exogenous fibrolytic enzyme application on the microbial attachment and digestion of barley straw in vitro. Asian-Aust. J. Anim. Sci. 25:66-74. https://doi.org/10.5713/ajas.2011.11158
  33. Williams, A. G. and N. H. Strachan. 1984. Polysaccharide degrading enzymes in microbial populations from the liquid and solid fractions of bovine rumen digesta. Can. J. Anim. Sci. 64:58-59. https://doi.org/10.4141/cjas84-156
  34. Windham, W. R. and D. E. Akin. 1984. Rumen fungi and forage fiber degradation. Appl. Environ. Microbiol. 48:473-476.