• Title/Summary/Keyword: reverse fraction

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In vitro Evaluation of Phalaris minor Seeds as Livestock Feed

  • Kaur, J.;Pannu, M.S.;Kaushal, S.;Wadhwa, M.;Bakshi, M.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.3
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    • pp.363-367
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    • 2006
  • The nutritional worth of Phalaris minor seeds was assessed in comparison to conventional cereal grains like maize and wheat. P. minor seeds had higher total ash and cell wall constituents as compared to wheat and maize grains. The CP content of P. minor was comparable to wheat grains but higher than maize grains. The in vitro studies revealed that the net gas production and availability of ME from P. minor was comparable to that of maize but the digestibility of nutrients was significantly (p<0.05) lower than that of conventional cereal grains. The digestion kinetic parameters for DM and CP revealed that P. minor had the highest (p<0.05) soluble fraction (a) followed by wheat and maize. Reverse trend was observed for insoluble but potentially degradable fraction (b). The effective and true DM and CP degradability was significantly (p<0.05) higher in wheat grains followed by that in P. minor and maize grains. The digestibility of OM and NDF was not affected by replacing cereal grains in concentrate mixture with P. minor seeds up to 75 per cent level. But the availability of ME from concentrate mixtures was comparable to control only up to 50% level of replacement. Replacement of cereal grains with P. minor did not affect the rapidly soluble fraction and insoluble but potentially degradable fraction of concentrate mixture containing P. minor up to 75 per cent, but it was depressed significantly at 100% replacement level. The effective and true degradability of DM of concentrate mixtures containing P. minor from 50 to 100 per cent was comparable to that of conventional concentrate mixture (CCM). The wheat based concentrate mixtures showed higher net gas production (208 vs. 201 ml/g DM/24 h), digestibility of nutrients and ME availability (9.64 vs. 9.54 MJ/kg DM) as compared to maize based concentrate mixture. The wheat based concentrate mixture had significantly (p<0.05) higher rumen undegradable fraction and effective degradability. The data conclusively revealed that conventional cereal grains could be replaced with P.minor seeds up to 75 per cent without affecting the availability of nutrients.

돼지 간장으로 부터 정제한 천연 단백성 Methylation Inhibitor의 S-Farnesylcysteine Methyltransferase 억제효과

  • 남석우;유세근;서동완;남태균;이향우;홍성열
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.262-262
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    • 1994
  • Ras oncogene의 산물로서 대부분의 암조직이나 transformed human cell에서 거의 공통적으로 발견되는 p21 단백질은 C-terminal processing에 의해 먼저 C-terminal cysyeine에 palmition 된 후 carboxylmethylation 된다. Palmitation은 transforming activity의 요건인 세포막에 대한 친화력을 유지시키기 위한것으로 추측되며, cysteine residue의 carboxylmethylation의 의미는 아직 확실히 밝혀지고 있지 않으나 세포막에 대한 친화력을 증가 시키는 것으로 추측되고있다. 본 연구에서는 S-Farnesylcysteine Methyltransferase의 기질로서 N-acetyl-S-trans, trans-farnesyl-L-cysteine(AFC)을 합성하였으며, 본 실험실에서 계속 연구하여 온 돼지 간장으로 부터 정제한 천연 단백성 Methylation Inhibitor의 S-Farnesylcysteine Methyltransferase 활성에 대한 억제효과를 검색하였다. 천연 단백성 Methylation Inhibitor는 돼지 간조직의 soluble fraction을 열처리하여 Sephadex G-25 column chromatngraphy한 후 reverse phase HPLC로 정제하였다. 본 inhibitor는 약 10개의 아미노산으로 구성된 peptide성 천연물질로 분자량은 1,400 Da 으로서 합성한 AFC를 기질로 하였을 때, 흰쥐 뇌 조직내 S-Farnesylcysteine methyltransferase에 대한 $IC_{50}$/은 0,82 $\times$ $10^{-6}$ M이었으며 또한 human cancer cell line의 S-Farnesylcysteine Methyltransferase에 대해서도 현저한 저해효과를 나타내었다.

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Behavior of geopolymer and conventional concrete beam column joints under reverse cyclic loading

  • Raj, S. Deepa;Ganesan, N.;Abraham, Ruby;Raju, Anumol
    • Advances in concrete construction
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    • v.4 no.3
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    • pp.161-172
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    • 2016
  • An experimental investigation was carried out on the strength and behavior plain and fiber reinforced geopolymer concrete beam column joints and the results were compared with plain and steel fiber reinforced conventional concrete beam column joints. The volume fraction of fibers used was 0.5%. A total of six Geopolymer concrete joints and four conventional concrete joints were cast and tested under reversed cyclic loading to evaluate the performance of the joints. First crack load, ultimate load, energy absorption capacity, energy dissipation capacity stiffness degradation and moment-curvature relation were evaluated from the test results. The comparison of test results revealed that the strength and behavior of plain and fiber reinforced geopolymer concrete beam column joints are marginally better than corresponding conventional concrete beam column joints.

Role of fibers on the performance of geopolymer concrete exterior beam column joints

  • Raj, S. Deepa;Ganesan, N.;Abraham, Ruby
    • Advances in concrete construction
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    • v.9 no.2
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    • pp.115-123
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    • 2020
  • The performance of steel fiber reinforced geopolymer concrete beam column joints under cyclic loading was investigated. The volume fraction of fibers considered were 0.25% (19.62 kg/㎥), 0.5% (39.24 kg/㎥), 0.75% (58.86 kg/㎥) and 1% (78.48 kg/㎥). A total of fifteen specimens were prepared and tested under reverse cyclic loading. Test results were analyzed with respect to first crack load, ultimate load, energy absorption capacity, energy dissipation capacity, stiffness degradation and load deflection behavior. Test results revealed that the addition of steel fibers enhanced the performance of geopolymer concrete beam column joints significantly. The joints were analyzed using finite element software ANSYS. The analytical results were found to compare satisfactorily with the experimental values.

Capacity analysis of CDMA cellular systems using erlang capacity (얼랑 캐패시티를 이용한 CDMA 셀룰라 시스템의 용량 분석)

  • 양정모;박진수
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.35S no.3
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    • pp.39-46
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    • 1998
  • In the FDMA and TDMA Cellular Systems, call blocking rise when frewquence slots andtime slots are used to ther users. Otherwise, in the CDMA cellular systems, call locking arises when interference level is above 10dB according to call of other users. System capacity is defined to be Erlang capacity that is the number of users when CDMA blocking probability is 1% or 2%. Users number corresponded channel number of AMPS and TDMA cellular systems. In this paper, we proposed new reuse-fraction using square of interference poser and obtained Erlang capacity for reverse link of CDMA cellular system with bit rate $R_{b}$, 9.6kbps and 14.4kbps. As a results, Erlange capacity of CDMA system have more 20.7 and 5.5 times than AMPS and TDMA system and have more form 1 to 4 Erlang thatn Viterbi and Padovani obtaned.d.

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A Study on the Measurement for the Recovery Stress of Intelligent Composite by Experiment (실험법에 의한 지능성 복합체의 회복응력 측정에 관한 연구)

  • Hawong, Jai-Sug;Lee, Hyo-Jae
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.27 no.4
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    • pp.515-523
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    • 2003
  • Shape memory is physical phenomenon which a platically metal is restored to its original shape by a solid state phase change by heating. TiNi alloy the most effective material in the shape memory alloy(SMA). To study(measure) recovery stress of intelligent composite. Ti50-Ni50 shape memory matrix with prestrain SMA fiber. When SMA fiber of the intelligent composite is heated over austenite starting temperature(As) by electric heating. a recovery stress are generated. The recovery stress of the intelligent composite was measured by strain gage or photoelastic experiment. Measuring method of recovery stress by photoelastic experiment was developed in this research. It was certified that photoelastic experiment was more effective and more precise than strain gage method in the measurement of recovery stress.

Effect of Partial-Band Interference in Multi-Carrier CDMA Network (다중 반송파 CDMA 네트워크에서 부분 대역 간섭의 영향)

  • Roh Jae-Sung
    • Journal of Digital Contents Society
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    • v.5 no.3
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    • pp.187-191
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    • 2004
  • Rapid time variations of the mobile communication channel have a dramatic effect on the performance of multi-carrier modulation. This paper analyzed the bit error rate (BER) performance for multi-carrier code division multiple access (MC-CDMA) network, and derived the equivalent signal-to-total noise plus interference(SNIR) of MC-CDMA system in the reverse link, the performance of MC-CDMA/BPSK signal is evaluated taking into account the fraction of partial-band interference and interference power. and the effect of partial-band interference is comprehensively studied in order to further improve the Qos of multi-carrier multimedia system.

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Studies on the Polysaccharide Extracted from Ganoderma Incidum (영지버섯에서 분리한 항암성 다당체에 관한 연구)

  • 김성환;김을상;김영식
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.1
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    • pp.147-153
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    • 1995
  • The cultured mycelial cells of Ganoderma lucidum was extracted by alkali, and then neutralized by acid. The extract was passed through the column of DEAE cellulose for more purification. The neutral fraction was concentrated and precipitated with 95% ethanol. The precipitate was lyophilized and then PSG(polysaccharides) was obtained. PSG was composed of 82.2% polysaccharide, 0.7% protein and 17.1% uronic acid. Sugar conjugates of its hydrolysates were produced using with fluorescent compound(7-amino-1,3-naphthalene disulfonic aicd : 7-AGA), and then fluorescent labeled sugar conjugates were separated by reverse phase high perfomance liquid chromatography. Hydrolysates of PSG were composed of sixteen amino acids and 95.7% glucose, 2.7% xylose, 1.6% fucose and tract amount of galactose and mannose. The immunomodulating effects of PSG on macrophage were perfomed using murine macrophage cell line ATCC TIB 71 cells. PSG augumented the phagocytic activity of TIB 71 cells against fluorescent latex beads.

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Three New Flavonoids Of Spatholobus suberectus

  • Yoon, Jeong-Seon;Lim, Song-Won;Yang , Hye-Kyeong;Ahn , Tae-Kyeong;Kim, Eun-Sil;Sung, Sang-Hyun;Kim, Young-Choong
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.202.1-202.1
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    • 2003
  • Six compounds were isolated from the 90% MeOH fraction of the vine stem of Spatholobus suberectus Dunn (Leguminosae) using silica gel, reverse phase column chromatography and RP-HPLC. Structures of compounds 1-6 were elucidated by spectroscopic parameters of IR, E1 MS, FAB MS, lD-NMR and 2D-NMR spectrum and identified as pseudobaptigenin (1), genistein (2), (2R)-7-hydroxy-6-methoxyflavanone (3), (3R,4R)-2",4"-dihydroxy-6,7-methylenedioxy-isoflavan-4-ol (4), (3R,4R)-7,2" -dihydroxy- 4"-methoxyisoflavan-4-ol (5), sativan (6), respectively. Compounds 3, 4 and 5 have been newly reported in nature.

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Separation and Purification of Angiotensin-I Converting Enzyme Inhibitory Peptides from Layer Hydrolysate (김 가수분해물로부터 Angiotensin-I Converting Enzyme저해 Peptide의 분리$\cdot$정제)

  • LEE Heon-Ok;KIM Dong-Soo;DO Jeong-Ryong;KWAN Dae-Young
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.2
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    • pp.164-172
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    • 2001
  • The angiotensin-I converting enzyme (ACE) inhibitors from laver hydrolysate was isolated. Among the 13 kinds of proteases, Maxazyme NNP was most effective for preparing the high ACE inhibitory compound. In extraction conditions of ACE inhibitory peptide from laver hydrolysate, ACE inhibitory activity of hydrolysate treated with diethylether for decolorization and that of $70\%$ ethanol soluble fraction among the different ethanol concentrations were higher than other preparations. Low molecular fraction less than 3,000 dalton of layer hydrolysate separated by ultrafiltration had the highest ACE inhibitory activity, for further separation of ACE inhibitory peptide from laver hydrolysate, gel filtration chromatography (Sephadex G-25), reverse-phase HPLC (ODS & Vydac C-18) and gel permeation chromatography (Superdex Peptide HR) were performed. The molecular mass of the ACE inhibitory peptide fractions of gel permeation chromatography determined by electrospray-mass spectrometer were 413.48 (S1O2V2V1P),346.86 (S1O2V2V2P) and 320.32 (S2O6V3V1P) dalton and their amino acid sequence were Val-Gln-Gly-Asn, Thr-Glu-Thr and Phe-Arg, respectively.

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