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Expression of Sodium-Iodide Symporter (NIS) in Thyroid Nodules: Comparison of RT-PCR and Immunohistochemical Staining Methods (갑상선 결절에서 Sodium Iodide Symporter (NIS)의 발현: RT-PCR방법과 면역조직화학염색법의 비교)

  • Bae, Sang-Kyun;Lee, Kang-Dae;Chang, Hee-Kyung
    • The Korean Journal of Nuclear Medicine
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    • v.38 no.6
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    • pp.511-515
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    • 2004
  • Purpose: The sodium-iodide symporter (NIS) expression is an important factor in determining the sensitivity of radioiodine therapy in well-differentiated thyroid cancers. Several previous studies for the expression of NIS in thyroid tissues show diverse results. To investigate whether there is difference between methods in determining the expression of NIS in thyroid tissues of patients with thyroid nodules, we measured the expression ot NIS using two different methods (RT-PCR and immunoshistochemical staining) and compared the results. Materials & Methods: We measured the expression of NIS by reverse transcriptase-polymerase chain reaction (RT-PCR) and also by immunohistochemical staining using anti-NIS antibody in thyroid cancers and other benign thyroid diseases. We compared the results of each method. We included 19 papillary carcinomas, 1 follicular carcinoma, 7 medullary carcinoma, 4 adenomas and 7 nodular hyperplasias. Results: By RT-PCR analysis, 10 of 19 papillary carcinomas expressed NIS, but 1 follicular cancer didn't express NIS. By immunohistochemical staining, 15 of 19 papaillary carcinomas express NIS, but 1 follicular lancer didn't express NIS. There was a significant correlation between the semiquautitative results of RT-PCR and immunohistochemical staining of NIS expression. (p<0.01) Conclusion: Our data demonstrated that the expression of NIS in thyroid cancers and other benign diseases investigated by RT-PCR and immunohistochemical staining correlated well each other. However, by immunohistochemical staining, more NIS expression was found.

A Study on the Solving Proportion Problems of Mathematics Textbooks and Proportional Reasoning in 6th Graders (초등학교 6학년 학생들의 교과서 비례 문제 해결과 비례 추론에 관한 연구)

  • Kwan, Mi-Suk;Kim, Nam-Gyunl
    • Journal of Elementary Mathematics Education in Korea
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    • v.13 no.2
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    • pp.211-229
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    • 2009
  • The purpose of this study is analysis of to investigate relation proportion problem of mathematics textbooks of 7th curriculum to proportional reasoning(relative thinking, unitizing, partitioning, ratio sense, quantitative and change, rational number) of Lamon's proposal at sixth grade students. For this study, I develop two test papers; one is for proportion problem of mathematics textbooks test paper and the other is for proportional reasoning test paper which is devided in 6 by Lamon. I test it with 2 group of sixth graders who lived in different region. After that I analysis their correlation. The result of this study is following. At proportion problem of mathematics textbooks test, the mean score is 68.7 point and the score of this test is lower than that of another regular tests. The percentage of correct answers is high if the problem can be solved by proportional expression and the expression is in constant proportion. But the percentage of correct answers is low, if it is hard to student to know that the problem can be expressed with proportional expression and the expression is not in constant proportion. At proportion reasoning test, the highest percentage of correct answers is 73.7% at ratio sense province and the lowest percentage of that is 16.2% at quantitative and change province between 6 province. The Pearson correlation analysis shows that proportion problem of mathematics textbooks test and proportion reasoning test has correlation in 5% significance level between them. It means that if a student can solve more proportion problem of mathematics textbooks then he can solve more proportional reasoning problem, and he have same ability in reverse order. In detail, the problem solving ability level difference between students are small if they met similar problem in mathematics text book, and if they didn't met similar problem before then the differences are getting bigger.

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Application of Multi-Dimensional Precipitation Models to the Sampling Error Problem (관측오차문제에 대한 다차원 강우모형의 적용)

  • Yu, Cheol-Sang
    • Journal of Korea Water Resources Association
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    • v.30 no.5
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    • pp.441-447
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    • 1997
  • Rainfall observation using rain gage network or satellites includes the sampling error depending on the observation methods or plans. For example, the sampling using rain gages is continuous in time but discontinuous in space, which is nothing but the source of the sampling error. The sampling using satellites is the reverse case that continuous in space and discontinuous in time. The sampling error may be quantified by use of the temporal-spatial characteristics of rainfall and the sampling design. One of recent works on this problem was done by North and Nakamoto (1989), who derived a formulation for estimating the sampling error based on the temporal-spatial rainfall spectrum and the design scheme. The formula enables us to design an optimal rain gage network or a satellite operation plan providing the statistical characteristics of rainfall. In this paper the formula is reviewed and applied for the sampling error problems using several multi-dimensional precipitation models. The results show the limitation of the formulation, which cannot distinguish the model difference in case the model parameters can reproduce similar second order statistics of rainfall. The limitation can be improved by developing a new way to consider the higher order statistics, and eventually the probability density function (PDF) of rainfall.

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Recycling Studies for Swine Manure Slurry Using Multi Process of Aerobic Digestion (MPAD) (다중 호기 소화공정을 이용한 양돈분뇨 슬러리의 자원화 연구)

  • Kim, Soo-Ryang;Yoon, Seong-Ho;Lee, Jun-Hee;Lee, Myung-Gyu
    • Journal of Animal Environmental Science
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    • v.15 no.2
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    • pp.155-160
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    • 2009
  • This study was carried out to investigate the feasibility of Multi Process of Aerobic Digestion (MPAD) for recycling of swine manure slurry as fertilizer. MPAD consisted of three kinds of difference process which are thermophilic aerobic oxidation (TAO) system, lime solidification system, and reverse osmosis (R/O) membrane system. TAO system was studied well previously for decade. The chemical composition of the lime-treated solid fertilizer was as like that organic matter 17.4%, moisture 34.1%, N 0.9%, P 1.7%, K 0.3%, Ca 12.7%, and which was expected to be useful as acid soil amendment material. The concentrated liquid material produced by R/O membrane system was also expected as a good fertilizer for crops production and soil fertility improvement.

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Effects of Changbudodam-tang on Obesity-induced Rats (창부도담탕이 비만 유도 흰쥐에 미치는 영향)

  • Kim, Soo-Hyeon;Lee, Eun-Kyu;Choe, Yoo-Jin;Jo, Seong-Hui;Yang, Seung-Jeong
    • The Journal of Korean Obstetrics and Gynecology
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    • v.34 no.1
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    • pp.48-65
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    • 2021
  • Objectives: This study was designed to evaluate the efficacy of Changbudodam-tang on obesity by using high-fat diet rats. Methods: Rats were divided into five groups. Normal group: Normal diet, Control group: High-fat diet, Positive control group: High-fat diet+Dietamin 4 mg/kg/day, Changbudodam-tang-Low group: High-fat diet+Changbudodam-tang 250 mg/kg/day, Changbudodam-tang-High group: High-fat diet+Changbudodam-tang 500 mg/kg/day. Weight, food intake were measured every week. After 7 weeks, total cholesterol, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol, Triglyceride, free fatty acid, aspartate aminotransferase, alanine aminotransferase, complete blood count were measured and messenger ribonucleic acid expression of adiponectin, peroxisome proliferator-activated receptor-γ, leptin were observed using Reverse transcription polymerase chain reaction of liver cells. Results: There was no difference in food intake between groups. Body weight tended to decrease compared with the Control group, but it wasn't statistically meaningfull. The total cholesterol, low density lipoprotein-cholesterol, Triglyceride, free fatty acid tended to decrease compared with the Control group. High density lipoprotein-cholesterol tended to decrease compared with the Control group, but it wasn't statistically meaningfull. White blood cell, red blood cell, hemoglobin, platelet, aspartate aminotransferase, alanine aminotransferase were not affected by Changbudodam-tang. The messenger ribonucleic acid expression of Adiponectin, peroxisome proliferator-activated receptor-γ, leptin, which are involved in the differentiation of adipocytes, was decreased compared with the Control group. Conclusions: Based on the results above, it is suggested that Changbudodam-tang can be applied to improving serum lipid levels in obese patients caused by high fat diets.

The effects of asking unexpected questions on general details and verifiable details (예상치 못한 질문이 진술의 세부정보와 확인 가능한 사실의 양에 미치는 영향)

  • Moon, Hyemin;Jo, Eunkyung
    • Korean Journal of Forensic Psychology
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    • v.11 no.3
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    • pp.349-370
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    • 2020
  • This study was to test the effects of unanticipated questions on the number of general and verifiable details. In addition, the number of verifiable details would discriminate truth-tellers and liars more accurately than the number of general details. In a 2(Veracity: truth vs. lie) X 2(Question type: Expected questions vs. Unexpected questions) mixed-design study, truth tellers(N=40) were asked to visit a cafe on campus and liars(N=40) were told to fabricated a story as if they visited the cafe. Then, participants were interviewed about their trip to the cafe and asked four questions(two anticipated questions: 'report the trip in detail', 'describe the place'; two unanticipated questions: 'recall in reverse order', 'report verifiable details'). Each participant's statements were transcribed and coded by trained graduate students for the number of general details and verifiable details. The results showed that truth-tellers mentioned significantly more general details than liars regardless of the question type. On the contrary, there was no significant difference between liars and truth-tellers in the number of verifiable details. High percentages of truth-tellers(62.5%) and liars(80.0%) were classified correctly based on the number of general details whereas only 45.0% of truth tellers and 62.5% of liars were accurately classified by the number of verifiable details. Liars were found to speak more words when asked to provide verifiable details compared to a general open question, but the number of general details did not seem to increase accordingly. The limitations of this study and future research directions were discussed.

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A Study on the Effect of Physical Upward and Downward Movement Experience on Psychological Judgements (신체의 상향·하향 이동경험이 심리적 판단에 미치는 영향에 관한 연구)

  • Lee, Luri;Lee, Seung-yon;Chung, Hyun Jung
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.8 no.4
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    • pp.183-196
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    • 2018
  • Studies that approach from the point of view that human thoughts or minds are dominated by behavior as well as that human behavior is dominated by thoughts or minds, have begun to attract attention from the late 2000s. The physical experience is reminiscent of a metaphorically connected abstract concept, which ultimately affects the judgment or evaluation of a particular object. However, studies that have been carried out so far have been limited to studies on the difference in perception and judgment depending on the objects to be viewed, the objects to be touched, and the objects to which they are carried. In this study, we tried to find out that the physical movement of the body in the upward or downward direction affects the psychological judgment differently. In the first experiment, a pair of words that were considered to be connected metaphorically was tested. In the second experiment, the subjects tried to solve the complicated calculation problem in a short time, and then they watched the video related to the upward movement or downward movement, and then proceeded to measure the psychological judgment. As a result, it was found that 'downward movement' of the body has a metaphorical connection with 'closure', while 'upward movement' is related to 'progress'. In the case of downward-experienced group compared to upward-experienced group, the reverse intentions of their own decision were low, and the confidences in their own decision and the expectations for performance were high.

The Role of Relational Agency in a Need-reality Colliding Situation (욕구-현실 충돌 상황에서의 주체성의 역할)

  • Seheon Kim ;Taekyun Hur
    • Korean Journal of Culture and Social Issue
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    • v.29 no.4
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    • pp.617-636
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    • 2023
  • The goal of this study was to explain the phenomenon of making efforts to overcome the need-reality collision as a cultural characteristic of Koreans. Specifically, we examined whether the behavior varies depending on the degree of relational agency in the situation where conflicts between one's needs and reality have occurred. To this end, a total of 217 participants participated in the online experiment, and the data of 156 participants were finally analyzed. After responding to the relational agency scale, the participants were exposed to a decision-making scenario in which conflicting factors existed. The scenario were about buying a house and making a wedding hall contract, and in each scenario, two important values were set to conflict with each other in the market. Participants read the scenario and entered the level they wanted for each value. After that, they encounter a situation in which he or she has not found the candidate site corresponding to the level he or she wants. Then, the participants responded to their willingness to make additional efforts themselves. As a result of the study, the degree of relational agency of the participants showed a positive relationship with the degree of additional effort. In addition, the degree of the desired level beyond the reality (expectancy discrepancy) showed a nonlinear (reverse U-shape) influence on the additional effort while controlling for individual difference. Furthermore, the interaction effect between relational agency and expectancy discrepancy was significant. Specifically, individuals with low agency did not have a significant relationship between the degree of expectancy discrepancy and the dependent variable, but individuals with high relational agency had a significant non-linear relationship between the degree of expectancy discrepancy and the dependent variable. Based on the results of the study, the role and function of Koreans' psychological characteristics (relational agency) in the scene of managing needs-reality collision were discussed.

What's Different about Fake Review? (조작된 리뷰(Fake Review)는 무엇이 다른가?)

  • Jung Won Lee;Cheol Park
    • Information Systems Review
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    • v.23 no.1
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    • pp.45-68
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    • 2021
  • As the influence of online reviews on consumer decision-making increases, concerns about review manipulation are also increasing. Fake reviews or review manipulations are emerging as an important problem by posting untrue reviews in order to increase sales volume, causing the consumer's reverse choice, and acting at a high cost to the society as a whole. Most of the related prior studies have focused on predicting review manipulation through data mining methods, and research from a consumer perspective is insufficient. However, since the possibility of manipulation of reviews perceived by consumers can affect the usefulness of reviews, it can provide important implications for online word-of-mouth management regardless of whether it is false or not. Therefore, in this study, we analyzed whether there is a difference between the review evaluated by the consumer as being manipulated and the general review, and verified whether the manipulated review negatively affects the review usefulness. For empirical analysis, 34,711 online book reviews on the LibraryThing website were analyzed using multilevel logistic regression analysis and Poisson regression analysis. As a result of the analysis, it was found that there were differences in product level, reviewer level, and review level factors between reviews that consumers perceived as being manipulated and reviews that were not. In addition, manipulated reviews have been shown to negatively affect review usefulness.

Effects of Ojeoksangamibang on the Lipid Metabolism, Anti-oxidation and Concentration of Proinflammatory Cytokines in Rat Fed High Fat Diet (오적산가미방(五積散加味方)이 고지방식이 유도 비만쥐의 지질대사, 항산화계 및 전염증성 cytokine 생산에 미치는 영향)

  • Kong, In-Pyo;Park, Won-Hyung;Cha, Yun-Yeop
    • Journal of Korean Medicine Rehabilitation
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    • v.21 no.4
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    • pp.23-40
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    • 2011
  • Objectives: This study was designed to examine the effects of extracts of Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) on the lipid lowering, anti-oxidation and concentration of proinflammatory cytokines and was investigated on hyperlipidemic rats. Methods: Male rats weighing $182.39{\pm}4.71g$ were fed high fat diet for 8 weeks and 36 rats(above 400 g) were divided into 4 groups. Each of 9 rats was divided a control group and experimental groups. We fed a control group of rats a basal diet and administered normal saline(100 mg/kg, 1 time/1 day) for 4 weeks. And we fed each experimental group of rats basal diet and administered an extract of Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) extracts(100 mg/kg, 200mg/kg, 300 mg/kg, 300 mg/kg, 1 time/1 day) for 4 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. We measured lipid of plasma and liver, concentration of proinflmmatory cytokines, anti-oxidative activity and $TNF-{\alpha}$, Apo-B, Apo-E and leptin gene expression. Results: 1. Concentration of plasma free fatty(FFA) showed no significant difference in all the treatment groups. Concentration of plasma triglyceride(TG) showed a significant decrement in the 300 mg/kg in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups than that of control group. 2. Concentration of plasma total cholesterol showed a significant decrement in the 200 and 300 mg/kg in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups than that of control group. Concentration of plasma low density lipoprotein(LDL)-cholesterol showed a Significant decrement in the 300 mg/kg in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups than that of control group. Concentration of plasma high density lipoprotein(HDL)-cholesterol showed a significant increment in the 300 mg/kg in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) group. 3. Concentration of liver total cholesterol showed a tendence to decrease in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups. Concentration of liver TG showed a significant decrement in all Ojeoksangamibang groups than that of control group. 4. Concentration of plasma and liver thiobarbituric acid reactive substance(TBARS) showed a tendence to decrease in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups. 5. The values of glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and catalase(CAT) activity showed a significant increment in all Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups than that of control group. 6. The values of plasma aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activity showed no significant different in all treatment group. 7. Concentration of plasma $interleukin(IL)-1{beta}$ showed no significant difference in all the treatment groups. Concentration of plasma IL-6 showed a significant decrement in the 300 mg/kg in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) group than that of control group. Concentration of plasma tumor necrosis $factor-{\alpha}(TNF-{\alpha})$ a siginifant decrement in the 200 and 300 mg/kg in Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) group than that of control group. However the concentration of plasma IL-10 in the 300 mg/kg Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups showed a significant increment than that of control group. 9. In the analysis of reverse transcription-polymerase chain reaction(RT-PCR), gene expression of $TNF-{\alpha}$, Apo-B and Apo-E in the Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups showed a lower expression than that of control group. However the gene expression of leptin showed no difference in the treatment groups. 10. The ratio of $TNF-{\alpha}$, Apo-B, and Apo-E per ${\beta}-actin$ expression in the Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) groups showed a significant decrement than that of control group. However The ratio of leptin expression per ${\beta}-actin$ expression showed no significant difference among all the treatment groups. Conclusions: According to above results, in lowering lipid effect, anti-oxidation and control of pro-inflammatory cytokines production, Ojeoksangamibang($W{\check{u}}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) gives effect.