This study examined several kinematic properties of the primary articulator (the tongue dorsum) and the supplementary articulator (the jaw) in the articulation of the voiceless velar stop (/k/) within nonassimilating contexts. We examined in particular the spatiotemporal properties (constriction duration and constriction maxima) from the constriction onset to the constriction offset by analyzing a velar (/k/) followed by the coronal fricative (/s/), the coronal stop (/t/), and the labial (/p/) in across-word boundary conditions (/k#s/, /k#t/, and /k#p/). Along with these measurements, we investigated intergestural temporal coordination between C1 and C2 and the jaw articulator in relation to its coordination with the articulation of consonant sequences. The articulatory movement data was collected by means of electromagnetic midsagittal articulometry (EMMA). Four native speakers of Seoul Korean participated in the laboratory experiment. The results showed several characteristics. First, a velar (/k/) in C1 was not categorically reduced. Constriction duration and constriction degree of the velar (/k/) were similar within nonassimilating contexts (/k#s/=/k#t/=/k#p/). This might mean that spatiotemporal attributes during constriction duration were stable and consistent across different contexts, which might be subsequently associated with the nontarget status of the velar in place assimilation. Second, the gestural overlap could be represented as the order of /k#s/ (less) < /k#p/ (intermediate) < /k#t/ (more) as we measured the onset-to-onset lag (a longer lag indicated shorter gestural overlap.). This indicates a gestural overlap within nonassimilating contexts may not be constrained by any of the several constraints including the perceptual recoverability constraint (e.g., more overlap in Front-to-Back sequences compared to the reverse order (Back-to-Front) since perceptual cues in C1 can be recovered anytime during C2 articulation), the low-level speech motor constraint (e.g., more overlap in lingual-nonlingual sequences as compared to the lingual-lingual sequences), or phonological contexts effects (e.g., similarity in gestural overlap within nonassimilating contexts). As one possible account for more overlap in /k#t/ sequences as compared to /k#p/, we suspect speakers' knowledge may be receptive to extreme encroachment on C1 by the gestural overlap of the coronal in C2 since it does not obscure the perceptual cue of C1 as much as the labial in C2. Third, actual jaw position during C2 was higher in coronals (/s/, /t/) than in the labial (/p/). However, within the coronals, there was no manner-dependent jaw height difference in C2 (/s/=/t/). Vertical jaw position of C1 and C2 was seen as inter-dependent as higher jaw position in C1 was closely associated with C2. Lastly, a greater gap in jaw height was associated with longer intergestural timing (e.g., less overlap), but was confined to the cluster type (/kp/) with the lingual-nonlingual sequence. This study showed that Korean jaw articulation was independent from coordinating primary articulators in gestural overlap in some cluster types (/k#s/, /k#t/) while not in others (e.g., /k#p/). Overall, the results coherently indicate the velar stop (/k/) in C1 was robust in articulation, which may have subsequently contributed to the nontarget status of the velar (/k/) in place assimilation processes.
The purpose of this study was to evaluate the morphology of mandibular symphysis and location of lower incisor under the influence of the craniofacial skeleton in skeletal Class III malocclusion. The sample consisted of 132 adults who have severe Class III malocclusion(prognathism group, 33 males and 33 females), and who have normal occlusion(normal group, 33 males and 33 females). They had not received any orthodontic treatment or orthognathic surgery. The lateral cephalograms were evaluated. The results were as follows : 1. Prognathism group were larger than normal group in comparison of facial skeleton (p<0.05) with the exception of ${\angle}FH-Pal$, ${\angle}SNA$. 2. In the morphology of symphysis, measurements of anteroposterior width(LaABBW, LiABBW, SW) of prognathism group were significantly less than that of normal group(p<0.001). 3. In the correlative analysis between the craniofacial skeleton and symphysis measurements of prognathism group, vertical measurements in relation with cranial base and mandibular plane showed reverse correlationship with anteroposterior width of symphysis(LiACBW, LaACBW, LiABBW, SW)(p<0.05). But, there was not distinct difference between horizontal skeletal measurements and symphysis measurements(p>0.05). 4. The probability by regression test between vertical measurements(${\angle}SN-Mn,\;{\angle}FMA,\;{\angle}Pal-Mn,\;{\angle}LFH$) and symphysis measurements(LiACBW, LiABBW, SW, ${\angle}LISA$) were very high(p<0.001).
This study was conducted to define the seasonal differences in the morphological characteristics, the photosynthetic abilities and the dark respirations of the 4-year old ginseng leaves. Chlorophyll-a content in the ginseng leaf was significantly decreased at September than at June but content of chlorophyll-b was not showed seasonal difference. At June, the amounts of chlorophyll a and b in the ginseng leaves grown in the back row were rather abundant than those grown in the front row, but no significant differencies were detected between rows at September. The estimated optimum light intensity for the photosynthesis of ginseng leaves was higher at June than at September and higher in the front row than the back row but was significantly decreased by air temperature above 25$^{\circ}C$. The light compensation point was elevated in higher temperature and at September than June. The amount of photosynthesis was significantly increased in the ginseng plant grown in the front row than the back row at June but the reverse was significant at September. The highest photosynthesis was observed in temperature range of 20 - 25$^{\circ}C$ at June and range of 15 -20$^{\circ}C$ at September. The optimum temperature range of photosynthesis was 21$^{\circ}C$ to 25$^{\circ}C$ at June and 14$^{\circ}C$ to 21$^{\circ}C$ at September, and that was higher in the back row than the front row. High temperature significantly stimulated the dark respiration of ginseng leaves and the respiratory quotients(Q$\_$10/) of the ginseng leaves showed a significant seasonal variation.
Background: This study was performed to investigate the impact of exogenous ghrelin on the pancreatic ${\alpha}$-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine. Methods: Sprague-Dawley male rats (9 weeks old, $300{\pm}10g$) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and $10.0{\mu}g/kg$ body weight (BW), respectively. The plasma ghrelin and cholecystokinin (CCK) level were determined using enzyme immunoassay kit; the mRNA expression of ghrelin receptor ($GHSR-1{\alpha}$) and growth hormone (GH) receptor were assessed by reverse transcription PCR; the expressions of pancreatic ${\alpha}$-amylase activity, extracellular-signal-regulated kinases (ERK), phosphorylated extracellular-signal-regulated kinases (pERK) and c-Jun N-terminal kinase (JNK) were evaluated by western blotting; moreover the responses of pancreatic proteins to ghrelin were analyzed using the two-dimensional gel electrophoresis system. Results: The exogenous ghrelin (1.0 and $10.0{\mu}g/kg\;BW$) elevated the level of plasma ghrelin (p < 0.05), and suppressed the expression of pancreatic ${\alpha}$-amylase at a dose of $10.0{\mu}g/kg\;BW$ (p < 0.05). No difference in the level of plasma CCK was observed, even though rats were exposed to any dose of exogenous ghrelin. In addition, a combination of western blot and proteomic analysis revealed exogenous ghrelin ($10.0{\mu}g/kg\;BW$) induced increasing the JNK and ERK expressions (p < 0.05) and four proteins such as Destrin, Anionic trypsin-1, Trypsinogen, and especially eukaryotic translation initiation factor 3 in rat pancreas. Conclusions: Taken together, exogenous ghrelin by i.p. infusion plays a role in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling pathway.
Kang Jin Ho;Ryu Yeong Seop;Yoon Soo Young;Jeon Seung Ho;Cho Sook Hyon
KOREAN JOURNAL OF CROP SCIENCE
/
v.49
no.6
/
pp.472-476
/
2004
Lateral roots formed on mungbean sprouts should lower their quality. The study was carried out to clarify the effects of aeration periods (AP; 1, 2, 3, and 4 hours) and temperature (AT; 20, 30, and $40^{\circ}C$) after 5 hour seed imbibition into 50 ppm benzyladenopurine (BA) solution on growth and morphological characters of mungbean (cv. Keumseongnogdu, Owoolnogdu and Zhong Lu 1) sprouts. On the 6th day, the mungbean sprouts were sorted by 4 categories on the base of hypocotyl lengths; > 7cm, 4 to 7cm, < 4cm, and non-germination to calculate their composition rates, number of lateral roots, lengths of hypocotyl and root, diameters at middle and upper parts of hypocotyl, fraction fresh and dry weights were measured. AP more affected growth of the cultivars than AT showing little effect on them. In the composition rate of the above 4 categories, cv. Zhong Lu 1 had the highest rate in longer than 7cm but nearly the same rate in AP treatments. Rates of longer than 4cm hypocotyls in cv. Keumseongnogdu, Owoolnogdu were increased with longer AP but their rates of shorter than 4cm showed reverse response to the former. Formation rate and number of lateral roots per sprout were decreased with longer AP, showing more severe decrement when delayed 3 to 4 hour AP. Upper part of hypocotyls and roots were more thickened and shortened in longer AP, respectively. Total fresh weights had no significant difference between AP treatments while hypocotyl fresh weights were increased with longer AP. It was concluded that in mungbean sprout culture aeration from BA treatment to the first watering permitted at least 4 hours.
Park, Byung-Gyu;Lee, Hyun-Sun;Jung, Sung-Hoon;Koo, Yun-Chang;Hong, Chung-Qui;Lee, Sun-Joo;Lee, Kwang-Won
Toxicological Research
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v.23
no.1
/
pp.79-86
/
2007
The isolated plantamajoside from Plantago asiatica that is often used as a marker compound in chemotaxonomic studies has various bioactivites such as the inhibitions of cyclic AMP phosphodi-esterase and 5-lipoxygenase, microbial growth and inflammation, and currently demands the generation of toxicity data. The purpose of this study was to examine the toxicities of the single and 14 days repeated dose toxicity in Sprague-Dawley rats orally administrated with plantamajoside at dose levels of 0, 500, 1000, and 2000 mg of dried material/kg body weight/day. The results showed that there was no difference in body weight change, food intake, water consumption, or relative organ weight among different dose groups. Also we observed no death and abnormal clinical signs were observed during the experimental period. Between the groups orally administered Plantago asiatica and the control group, there was no statistical significance in hematological test or serum biochemical values. There were no gross findings at final sacrifice. There was no evidence of histopathological alteration mediated by 14 days treatment with Plantago asiatica. These results suggest that no observed adverse effect level (NOAEL) of the oral application was considered to be more than 2000 mg/kg in rats under the conditions employed in this study. Another observation was performed to investigate the safety of Plantago asiatica in respect of genotoxicity. This substance was examined that Salmonella typhimurium reversion assay (Ames test) in strain TA98, TA100, TA1535. In the reverse mutation test, Plantago asiatica did not induce mutagenicity in Samonella typhimurium with and without metabolic activation. These results indicated that Plantago asiatica had no genotoxicity.
A total of 1,440 White Leghorn pullets hatched in summer and winter, aged 20 to 72 weeks were fed 9 rations differing in dietary protein (13, 15 and 17%) and energy (2,500, 2,700 and 2,900 kcal/kg) levels for a period of 52 weeks in order to evaluate the optimum dietary energy and protein levels for laying hens. As metabolizable energy level increased from 2,500 to 2,900 kcal/kg of feed egg production, daily feed and protein intake and egg shell quality decreased, but reverse was true for the daily energy intake, energy requirement and feed cost per kg egg, body weight gain, nutrients utilizability and abdominal fat accumulation, Egg weight, viability and egg yolk Pigmentation were not affected by the dietary energy level. On the other hand, as dietary protein level increased from 13 to 17%, egg production, egg weight, daily protein intake, protein requirement per kg egg and body weight gain icreased, but daily feed and energy intake, feed and energy requirement per kg egg, egg yolk pigmentation and dry matter utilizability decreased, and no significant difference in the feed cost per kg egg, viability and egg shell quality was observed among dietary protein levels. However: the hens fed 15% and 17% Protein diets did not show significant differences in egg production, egg weight and body weight gain. For the entire laying period of 52 weeks, metabolizable energy level of 2,500 kcal/kg of feed and 15% dietary protein level were considered to be adequate to support the optimum productivity.
This study was conducted to estimate the validity of reverse transcriptase-polymerase chain reaction(RT-PCR) compared to enzyme immunoassay(EIA) for the detection of hepatitis C virus (HCV) infection. EIA for antibody to HCV(anti-HCV) and RT-PCR for HCV was executed on the subjects from Pusan and Kyungnam area with questionnaire survey to collect some relating factors of HCV infection. As the result from 617 cases, the prevalence of HCV infection was 1.5% by EIA and 3.7% by RT-PCR(p<0.05), and the age standardized rate was 1.7% and 3.4% by EIA and RT-PCR, respectively. The prevalence of hepatitis B surface antigen(HBsAg) was 6.8% by enzyme linked immunosorbent assay(ELISA) and the age standardized rate was 7.7%. It was the higher in male group comparing to female group(p<0.01). Both of the prevalence of HCV and HBsAg were higher in elevated asparate aminotransferase(AST) and alanine aminotransferase (ALT) group than in normal AST and ALT group(p<0.01). There was no specific risk factor of HCV infection. Though the degree of agreement of EIA and RT-PCR by gamma statistics was 97.2%, it showed a significant difference between the two methods(p<0.01). For the detection of HCV infection, positive predictive value of EIA was 66.7% and negative predictive value of EIA was 97.2%. This study suggests that negative result to anti-HCV by EIA didn't mean the free state of HCV infection, therefore it would be helpful that further monitoring for HCV infection by RT-PCR in the case of elevated AST and ALT and/or clinically suspected.
Paik, Nam Won;Kong, Sang Hui;Park, Jeong Im;Lee, Young Hwan
Journal of Korean Society of Occupational and Environmental Hygiene
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v.6
no.1
/
pp.97-108
/
1996
A new type of passive samplers were designed and produced by authors. After evaluating the quality of activated carbon by measuring recovery rate of organic vapors and steadiness of sampling rate, activated carbon with 30 - 35 mesh produced by Company S in Korea was selected. In each passive sampler, an amount of 400 mg of the activated carbon was filled in 25-mm cassette and covered by fixed screen (or wire screen with 100 mesh). In addition to the fixed screen, a wind screen (or wire screen with 300 mesh) was also attached at outer face. The sampling rate of the new Korean passive samplers was estimated Conclusions obtained in the study are as follows. 1. Sampling rates of the newly developed Korean passive samplers were affected by sampling time. For n-hexane, sampling rates of 15- and 60-minute samples were 70.92 and 37.45 ml/min, respectively. Sampling rate of both 200- and 450-minute samples was 25.96 ml/min. It is concluded that, when passive samplers are used for measuring organic vapors, samples be collected longer than 60 minutes. 2. Sampling rate of the passive samplers was also affected by airborne concentration of organic vapors. Lower sampling rates were determined at level of 1/2 threshold limit values (TLVs) recommended by the American Conference of Governmental Industrial Hygienists (ACGIH). It is recommended that sampling rate of the passive samplers be obtained at site by measuring concentrations using both the NIOSH Method and passive samplers simultaneously. 3. When the passive samplers, which collected organic vapors, were exposed to clean air for five hours, there was no significant loss of organic vapors due to reverse diffusion. 4. When samples were stored at room temperature ($21.8{\pm}0.7^{\circ}C$) and refrigerator ($3.8{\pm}0.7^{\circ}C$), there was no significant difference in the accuracy of results. For trichloroethylene and n-hexane, accuracies were within 25 % at both temperatures until seven days. However, poor accuracy exceeding 25 % was indicated in toluene from the first day. It is recommended that samples be stored at freezing temperature below $0^{\circ}C$. 5. Sampling efficiency was significantly affected by direction of the passive samplers. Results of samplers facing wind and down, respectively, were compared. Lower amount of organic vapors were collected when the sampler was oriented down. It is recommended that, when air velocity is low in plants, the passive samplers be oriented to the wind. However, when air velocity is high, the passive samplers be oriented down.
Experiment was conducted to get the information about physico-chemical properties of flue-cured tobacco on the degree of maturity cultivated in paddy-upland rotated field, and compared to upland ones. For the samples of this experiment, 3~4 leaves at each stalk position were harvested from the bottom of plants. Physico-chemical properties of cured leaves were determined from the samples collected at weekly intervals, and of obtained from 4 stalk positions. For the degree of maturity, harvested leaves were separated with visual characters into four classes such as immature, mature, ripe, and mellow. Regardless of stalk position, the order of shrinkage rate with length and width of leaves was mellow> immature> ripe> mature, and ripe leaves from paddy field showed higher shrinkage rate than those of upland. Nicotine and total nitrogen contents were decreased with the degree of maturity while reducing sugar content were showed a reverse tendency. Ripe leaves from paddy field had lower reducing sugar contents, comparing with upland tobacco. Filling capacity of cured leaves from paddy field was decreased with degree of maturity, but there was no difference between upland and paddy tobacco. Shatter index was increased in the oeder of immature > mellow > mature > ripe. Chemical components of cigarette smoke from paddy field tobacco were little higher in $CO_2$ total particulate matter and tar contents, while combustibility was little lower than that of upland tobacco. It was also evaluated that paddy field tobacco was unfavorable for the non-volatile organic and higher fatty acids contents comparing with upland tobacco.
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