• Journal of Food Hygiene and Safety
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• v.37 no.2
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• pp.39-45
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• 2022

This study aimed to investigate the validation and modify the analytical method to determine quercetin-3-𝑜-gentiobioside and isoquercitrin in Abelmoschus esculentus L. Moench for the standardization of ingredients in development of functional health products. The analytical method was validated based on the ICH (International Conference for Harmonization) guidelines to verify the reliability and validity there of on the specificity, linearity, accuracy, precision, detection limit and quantification limit. For the HPLC analysis method, the peak retention time of the index component of the standard solution and the peak retention time of the index component of A. esculentus L. Moench powder sample were consistent with the spectra thereof, confirming the specificity. The calibration curves of quercetin-3-𝑜-gentiobioside and isoquercitrin showed a linearity with a near-one correlation coefficient (0.9999 and 0.9999), indicating the high suitability thereof for the analysis. A. esculentus L. Moench powder sample of a known concentration were prepared with low, medium, and high concentrations of standard substances and were calculated for the precision and accuracy. The precision of quercetin-3-𝑜-gentiobioside and isoquercitrin was confirmed for intra-day and daily. As a result, the intra-day precision was found to be 0.50-1.48% and 0.77-2.87%, and the daily precision to be 0.07-3.37% and 0.58-1.37%, implying an excellent precision at level below 5%. As a result of accuracy measurement, the intra-day accuracy of quercetin-3-𝑜-gentiobioside and isoquercitrin was found to be 104.87-109.64% and the daily accuracy thereof was found to be 106.85-109.06%, reflecting high level of accuracy. The detection limits of quercetin-3-𝑜-gentiobioside and isoquercitrin were 0.24 ㎍/mL and 0.16 ㎍/mL, respectively, whereas the quantitation limits were 0.71 ㎍/mL and 0.49 ㎍/mL, confirming that detection was valid at the low concentrations as well. From the analysis, the established analytical method was proven to be excellent with high level of results from the verification on the specificity, linearity, precision, accuracy, detection limit and quantitation limit thereof. In addition, as a result of analyzing the content of A. esculentus L. Moench powder samples using a validated analytical method, quercetin-3-𝑜-gentiobioside was analyzed to contain 1.49±0.01 mg/dry weight g, while isoquercitrin contained 1.39±0.01 mg/dry weight g. The study was conducted to verify that the simultaneous analysis on quercetin-3-𝑜-gentiobioside and isoquercitrin, the indicators of A. esculentus L. Moench, is a scientifically reliable and suitable analytical method.

• Journal of Nutrition and Health
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• v.9 no.2
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• pp.59-67
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• 1976

A series of experiment was carried out to study the effect of commonly used dietary fat or oils on the growth, feed efficiency, nutrient utilizability, nitrogen retention and serum cholesterol of rats and chicks fed various fat or oils at the level of 10% during 12 weeks of experimentation. Fat and oils used in this experiment were also analyzed for the composition of some fatty acids. The main observations made are as follows: 1. All groups received fat or oils gained more body weight than unsupplemented control group except chicks fed fish oil and rapeseed oil although no statistical significance was found between treatments. It was found that body weight gain achieved by the rats fed soybean oil, rapeseed oil, animal fat or corn oil was much greater than other group and that achieved by the chicks fed corn oil and animal fat was greater than other vegetable oil groups, although no statistical significance was found among treatments. 2. Feed intake data indicated that corn oil group of both rats and chicks consumed considerably more feed than other groups. Whereas feed intake of fish oil groups was the lowest among the experimental animals indicating that fish oil might contain unfavorable compound that depresses the palatability. In feed efficiency, soybean oil group of rats and corn oil group of chicks were significantly better than other experimental groups. In general, addition of fat or oils in the diet improved feed effeciency of diet. 3. Nutrient utiIizabiIity and nitrogen retention data showed that fat in the experimental diet containing 10% fat or oils was absorbed better than crude fat in control diet. It was also found that there was no significant difference in nitrogen retention among treatment. 4. Liver fat content of rapeseed oil group was much higher than that of control group and other group. It was also noticed that feeding more polyunsaturated fatty acids resulted in higher content of Iiver fat. 5. Present data indicated that serum cholesterol content of rapeseed oil and sesame oil group of rat was the higher than that of control group. Serum cholesterol content of animal fat group of chicks was higher than other group. It was interesting to note that serum cholesterol content of chicken was higher than that of rats?regardless of the kind of oils received. 6. Analytical data revealed that fatty acid composition of vegetable oil was composed mainly of oleic acid and linoleic acid, whereas animal fat and fish oil were composed of saturated fatty acid such as, myristic and palmitic acid. It should be mentionted that the perilla oil contained a very large amount of linolenic acid (58.4%) comparing with that in order vegetable oils. Little arachidonic acid was detected in vegetable oil, whereas none in animal fat and. fish oil.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.2
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• pp.93-100
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• 2007

This study was conducted to determine the effects of different levels (10, 12 and 15%) of crude protein (CP) in total mixed ration (TMR) on dry matter intake, digestibility and nitrogen balance of Korean black goats in the stage of early pregnancy and to obtain information on their optimal dietary levels of CP. In the present study, 12 Does of Korean black goats in the early pregnancy were allotted to four unreplicated groups by dietary level of CP and then they were housed in individual metabolism cages with completely randomized design throughout 30 days with 20 days adaptation and 10 days collection periods. Does in Control were fed a conventional diet and does in TMR10, TMR12 and TMR15 were fed a diet adjusted to about 10, 12 and 15% CP, respectively. Dry matter(DM) contents ranged from 89 to 91% in treatments. There were no differences fur fiber contents among three CP levels of TMR, showing that ADF and NDF had 18.57 to 19.85, and 53.41 to 54.80, respectively. Crude protein contents for three TMR treaements had 10.61, 12.15 and 14.97%, respectively. However, non-fibrous carbohydrate (NFC) contents decreased with increasing CP levels in treatments. Meanwhile, Intakes of DM, nutrients and digestible nutrients were significantly (p<0.05) higher in TMR15 and control than in TMR10 and TMR12. Moreover, DM intake per metabolic body weight and theit ratio per body weight was significantly (p<0.05) higher for control and TMR15 than other treatments. DM digestibility was not significantly different among treatments, but ether extract digestibility of treatments was significantly (p<0.05) higher than that of control, but there was no significant difference among treatments. Nitrogen retention significantly (p<0.05) increased with increasing CP levels in TMR, and TMR15 was highest among treatments. Our results showed that the increasing CP levels in TMR increased DM intake and nitrogen retention and suggested that the optimal dietary CP levels under TMR feeding system in early pregnant Korean black goats could be estimated for at least 15%.

• The Journal of Korean Obstetrics and Gynecology
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• v.26 no.4
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• pp.30-47
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• 2013

Objectives: The purpose of the present study is to investigate anti-depressive effects of Samul-tanggahyangbuja (SGH) on ovariectomized and chronic mild stress (CMS) induced rats. Methods: Ovariectomized rats were exposed to CMS for 4 weeks. Changes of depression behavior were tested by using sucrose intake test (SIT), elevated plus maze (EPM), forced swimming test (FST) and Morris water maze test (MWMT) in rats until being orally medicated with SGH (100 or 400 mg/kg/day). In addition, the serum levels of corticosterone (CORT), IL-4, IL-$1{\beta}$ and changes of 5-HT in the brain were measured. Results: 1. SGH 400 mg/kg treated group (SGH 400) significantly increased amount of sucrose intake compared with the control group (p<0.05). 2. SGH 100 mg/kg treated group (SGH 100) and SGH 400 significantly increased the time spent in the open arms of the EPM compared with the control group (p<0.01). SGH 400 also significantly increased the number of crossing of the open and closed arms compared with the control group (p<0.05). 3. SGH significantly shortened the immobility time in FST compared with the control group (SGH 100 p<0.05, SGH 400 p<0.01). 4. SGH significantly increased performance of acquisition trials compared with the control group (p<0.05, on day 4, 5 of SGH 100 and 400). SGH 400 also significantly increased performance of retention trials compared with the control group (p<0.05). 5. The serum levels of corticosterone and IL-4 were not significantly different among the groups. There were no changes on the serum levels of corticosterone, IL-$1{\beta}$ and IL-4 after administration with SGH. 6. SGH 400 significantly increased the level of 5-HT in the hippocampus compared with the control group (p<0.05). SGH significantly increased the levels of 5-HT in the hypothalamus compared with the control group (SGH 100 p<0.05, SGH 400 p<0.01). Conclusions: These results suggest that SGH has the anti-depressive effect on ovariectomized rat and affect 5-HT system rather than hypothalamic-pituitary-adrenal (HPA) axis and immune system.

• The korean journal of orthodontics
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• v.40 no.2
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• pp.106-114
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• 2010

Objective: The aim of this experimental study was to evaluate the effects of direct electrical current stimulation (DECS) on bone regeneration in response to an expansion of the inter-premaxillary suture in the rat. Methods: Sixteen 50 - 60 days old Wistar male rats were separated into two equal groups (control and experimental). Both groups were subjected to expansion, and 30-gram of force was applied to the maxillary incisors with helical-spring. In the experimental group, two metallic-screws were placed at lateral parts of the maxillary segments. Electrodes were connected to the screws. The device was activated with current adjustment to measure $10{\mu}A$ continuously and the current was monitored daily during the expansion and early-retention phase. Bone regeneration in the sutural area was histomorphometrically evaluated including new-bone area (${\mu}m^2$), bone perimeter (${\mu}m$), feret's diameter (${\mu}m$) and newly formed bone (%) parameters. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p < 0.05 level. Results: Statistical analysis showed significant differences between groups for all investigated histomorphometric parameters. New bone area (p = 0.002), bone perimeter (p = 0.004), feret's diameter (p = 0.002) and newly formed bone percentage (p = 0.002) measurements were significantly higher in the experimental group than the control group. Bone histomorphometric measurements revealed that bone architecture in the DECS group was improved. Conclusions: The application of DECS to an orthopedically expanded inter-premaxillary suture area during the early retention phase stimulated the formation of new bone.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.3
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• pp.147-154
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• 2006

This study was conducted to investigate the effects of Total Mixed Ration(TMR) with wet brewer's grain on feed intake, nutrient digestibility and nitrogen retention of breeding Korean native goat. Twelve breeding Korean native goats were divided into four treatment groups, which were fed TMR containing 20, 30, 40% of wet brewer's grain and a control group fed concentrate feed and hay, respectively. Results are summarized as follows. Dry matter contents of TMR with wet brewer's grain was 61.46-65.81%, that of crude protein was 14.42-15.59%, ADF and NDF were 28.32-28.52, 53.46-54.16%, each. These were not different by supplemental level of brewer's grain in TMR. However, NFC contents were lower according to increase wet brewer's grain in TMR. Dry matter intake of TMR supplemented with 20% of brewer's grain was tend to be higher than those of control group. CP and digestible CP intake of TMR diets supplemented wet brewer's grain was intended to higher than those of a control group. NFC and digestible NFC intake were significantly higher in control group than in WBG40 treatment(p<0.05). Digestibilities of DM, ADF, NDF and NFC in control group were higher than those from TMR with wet brewer's grain(p<0.05). Digestibilities of CP of TMR with supplemented wet brewer's grain was tend to be higher than those of a control group. Nitrogen retention of TMR supplemented wet brewer's grain was tend to be somewhat higher compared to the control group

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.199-206
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• 2006

This study was conducted to investigate the effects of Total Mixed Ration (TMR) with wet brewer's grain on feed intake, nutrient digestibility and nitrogen retention of castrated Korean black goat. For feeding trial, forty castrated Korean black goats were divided into four treatment groups, which were fed TMR containing 20, 30, 40% of wet brewer's grain. For digestibility trial, twelve castrated Korean black goats were allotted to treatments in four groups of three goats. Results are summarized as follows. Dry matter (DM) contents of TMR with wet brewer's grain was $63.35{\sim}66.02%$, that of crude protein was $14.49{\sim}15.36%$, Acid detergent fiber (ADF) and Neutral detergent fiber (NDF) were $28.24{\sim}29.08,\;53.27{\sim}54.85%$, each. These were not different by supplemental level of brewer's grain in TMR. However, Non-fibrous carbohydrate (NFC) contents were lower according to increase wet brewer's grain in TMR. Average daily gain of control group were higher than those from TMR with wet brewer's gain (p<0.05). Intake of DM, Organic matter (OM) and NFC of TMR supplemented with 20% of brewer's grain were higher than those of other treatments (p<0.05). Crude protein (CP) and digestible CP intake of TMR diets supplemented wet brewer's grain was intended to higher than those of a control group. Digestibilities of DM, OM and NDF in control group were higher than those from TMR with wet brewer's grain (p<0.05). Digestibilities of CP of TMR with supplemented wet brewer's grain was tend to be higher than those of a control group. Nitrogen retention of TMR supplemented wet brewer's grain was tend to be somewhat higher compared to the control group

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.4
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• pp.135-149
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• 2020

As youth employment has become a social issue every year, the government is pushing for policies to support youth start-ups to create jobs voluntarily as a way to enhance the youth employment rate. In the case of young people in Gangwon Province, the number of people moving to other regions is increasing. This research is intended to empirically analyze the actual achievements of youth start-ups through the 'Youth Start-up Project' in Gangwon-do. It was divided into four categories: participation in government support, education completion, intellectual property right retention, and certification retention, which are characteristics of start-up companies, and hypotheses that they will have a positive impact on start-up performance (sales amount, duration of existence, or whether they are retained or not). Age and geographical factors (Yeongdong and Yeongseo) were injected as control variables to see how they affect them. Furthermore, empirical analysis was conducted by setting up a hypothesis that the characteristics of start-up companies and subsequent support between start-up performance would have a positive intermediary effect. The research results showed that the remaining characteristics, except for education completion, had a positive impact on sales, and that the more participation in government projects, the longer the duration of the company's existence. In addition, the level of participation in government support projects was significant in the direction of the government. The analysis results of the parameter, follow-up support, had a positive impact on the start-up performance, and the subsequent support mediating effect showed the mediating effect of the start-up performance, except for geographical factors. The results of this study suggest the need for customized support suitable for the characteristics of youth start-ups in order to enhance the performance of young start-ups. Support agencies need to refer to corporate characteristics for smooth management and selection. In the Gangwon-do area, the government should seek to provide timely and organic support for start-up companies in order to produce successful start-up cases.

• The Journal of Korean Academy of Prosthodontics
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• v.49 no.1
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• pp.29-37
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• 2011

Purpose: In cases when implant supported overdenture is made by using standard size implant, additional procedure such as bone surgery and bone grafting can be required. And it gives burden to doctor and patient in terms of cost. Therefore, it is necessary to find the implant therapy for the edentulous patients in making denture with accordable cost and simple procedure. Materials and methods: Edentulous patients with upper and lower dentures participated in this study. Before the operation, survey about patient's satisfaction to the existing dentures was carried out. Surgical procedures included four small diameter implants installation anterior area and immediate loading. One and three month after the procedure, the same survey about patient's satisfaction was carried out, and radiography was taken. Results: We are doing research to the nine patients. Survival rate is 97.2 percent. The comparison of patient's satisfaction before and after surgery is performed based on oral health impact profile 49. We analyze mainly with masticatory discomfort, retention, aesthetics, social problem, psychological discomfort problems. As a result, satisfaction level is increased at all factors. Retention is the most increased satisfactory factor followed by mastication difficulty, pronunciation, psychological discomfort, social discomfort, aesthetics in order. Marginal bone loss is 0.21 mm at 12 weeks after implant placement. Conclusion: This research reveals that the denture supported by mini dental implant increases patient's satisfaction. This study will be continued with more patients for a long time and we are scheduled for taking additional radiography to check whether peri-implant bone resorption occurs or not.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.5
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• pp.586-594
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• 1996

This study was designed to determine the level of soybean meal (SM) that could be substituted for fish meal in the diet for Korean rockfish (Sebastes schlegeli). A control diet with white fish meal as only protein source was included, SM was substituted at levels of $5\%,\;10\%,\;15\%,\;and\;20\%$ for portions of the fish meal in the diet. Amino acids (met+lys) supplementation and two different SM particle sizes (0.5 mm and 0.25 mm) in the $20\%$ SM diet were compared. In addition, combination of $10\%$ SM, $10\%$ corn gluten meal (CGM) and $10\%$ meat and bone meal (MBM) was substituted for the fish meal in the diet. Duplicate groups of the 30 fish averaging 22.9 g were fed one of 8 isoproteic $(48\%)$and isolipidic $(7.5\%)$ experimental diets to satiation twice a day for 9 weeks. No significant differences were found among fish fed the control, substituting up to $20\%\;SM,\;20\%$ SM containing amino acids, and $10\%\;SM+10\%\;CGM+10\%$ MBM diets in weight gain, feed efficiency, daily feed intake, and protein retention (P>0.05). Feed efficiency, daily feed intake and protein retention in fish fed the diet containing $20\%$ SM with 0.5 mm particle size were not significantly different to those in fish fed the diets containing less than $20\%\;SM\;(P>0.05)$, however, weight gain was significantly lower than that of the control and $5\%\;SM\;diets\;(P<0.05)$. It is concluded that SM ran be used as a partial substitute for fish meal up to $20\%$ in the diet, and that the inclusion of SM with an adequate combination of several protein sources can replace larger amounts of fish meal in the diet.