• The Korean Journal of Malacology
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• v.27 no.4
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• pp.323-330
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• 2011

We have developed a polyculture container which is preferable for rearing of abalones and sea cucumbers in East Sea. To test the rearing capacity of the polyculture container, 50, 75 and 100 sea cucumbers ranging from the body length of 7-9 cm were cultured in three containers including 500 abalones with the shell length of about 5 cm, respectively. It was revealed that preferable density for sea cucumbers was 52-72 individuals in the polyculture container. Glutamate oxaloacetate transaminase and glutamate pyruvate transaminase were analyzed with Reflotron kit to investigate the health degree of abalones in two polyculture containers including 300 and 500 abalones with the shell length of about 5 cm. Glutamate oxaloacetate transaminase has been an important tool to know myocardial infarction, disease of liver, and destruction of muscle. Next experiment was conducted to determine the effects on growth of abalones and sea cucumbers in polyculture container. Experimental findings, RNA/DHA ratio, DNA and RNA contents (ug/mg) were not significantly different among all groups. The results imply that the method of polyculture can be rearing with sea cucumber without growth retardation of abalone. Production ability between polyculture container and the container used in south sea were carried out using suspended culture method during 8months. It was revealed that abalones and sea cucumbers are faster growing in polyculture container than in container used in south sea. Therefore, polyculture container is considered more appropriate for the abalones and sea cucumbers culture in East Sea.

• Journal of Yeungnam Medical Science
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• v.3 no.1
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• pp.151-160
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• 1986

The authors studied depression using Zung's Self-Rating Depression Scale(SDS), in the subjects of 3,499 male and 1,335 female college freshmen of Yeungnam University. The authors collected the reports of SDS during the periods from January to February, 1986, and applied ANOVA and t-test on depression scores in order to compare them between various psychosocial factors, and sexes. The results are as follows : There was significant difference in the mean averages of total depression scores between male and female students: male students scored 35.68+7.46, female students scored 38.93+7.26(P<0.001). The depression scores relating to the items of indecisiveness, psychomotor retardation, decreased libido, and diurnal variation were relatively higher in both groups. One hundred and sixty-one male students(4.6%) showed seriously high degree depression scores of 50 or higher, while one-hundred and seventeen female students(5.8%) showed the same scores. Female students attending in liberal arts showed higher level of depression scores(P<0.01). There was a strong tendency toward higher depression scores in the student who were dissatisfied with their home atmosphere, colleges, department and familiarity of parents, and those who had pessimistic views of self image in the past, present or future in both groups(P<0.001).

• Journal of Digital Convergence
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• v.11 no.6
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• pp.235-244
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• 2013

This research was performed to investigate the effects of participation in aqua exercise on blood composition and vascular compliance of mentally retarded persons. The targets of this research consisted of a control group of 12 people and an aqua exercise group of 12 people after selecting 24 mentally retarded males and aqua exercise group carried out an exercise program for 60 minutes per day and five days a week for 9 weeks. Weight, body fat percentage, blood composition and vascular compliance were measured in a preliminary inspection and the same items were measured in a post-inspection. The results of this measurement were as follows. Firstly, aqua exercise group showed a significant decrease in weight and body fat percentage (p<.05). Secondly, for blood composition, aqua exercise group showed a notable decrease in Total-Cholesterol, Triglyceride, and LDL-Cholesterol (p<.05) and a significant increase in HDL-Cholesterol (p<.05). Thirdly, aqua exercise group showed a notable increase in blood composition for both upper limbs and lower limbs (p<.05). Taken these results together, it was considered that a participation of mentally retarded persons in aqua exercise would prevent adult diseases and would have an affirmative influence on improvement of the quality of life.

• KSBB Journal
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• v.21 no.6 s.101
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• pp.433-438
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• 2006

A fibrinolytic enzyme gene (BCF-1) was subcloned to the pEB vector which is high expression vector in the Bacillus host. The enzyme was purified by using FPLC after ammonium sulfate precipitation. The enzyme was oral-administrated to the rat and checked the bleeding time, blood clotting time and fibrinolytic effect of the serum. In the bleeding time retardation test, it was longer about 1.7 fold in the feeding rat than without feeding. The serum of rat feeded with the enzyme had the fibrinolytic activity from 1 hour to 3 hours after oral-administration. After 3 hours from feeding, the fibrinolytic activity was decreased gradually. Also blood clotting time after bleeding was longer than that of control rat. The enzyme could be detected at band of 30,000 Da in the blood by western blotting. The enzyme was not harmful to the all internal organs of the rats. Taken together, the enzyme originated from B. subtilis BB-1 can be a candidate to develop the drug for thrombosis, arteriosclerosis and myocardial infarction.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7297-7301
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• 2014

Objective: To explore the radiosensitization effect of overexpression of silent information regulator 6 (SIRT6) on A549 non-small cell lung cancer (NSCLC) cells. Methods: Adenovirus vector Ad-SIRT6 causing overexpression of SIRT6 was established. Western blotting and MTT assay were adopted to detect the level of SIRT6 protein and the inhibitory rate of A549 cell proliferation after different concentrations of adenovirus transduction (0, 25, 100, 200, and 400 pfu/cell) for 24 h. Control group, Ad-null group and Ad-SIRT6 group were designed in this experiment and virus concentration of the latter two groups was 200 pfu/cell. Colony formation assays were employed to test survival fraction (SF) of the 3 groups after 0, 2, 4, 6, 8, 10 X-ray irradiation. Flow cytometry was used to detect the status of cell cycle of 3 groups after 48 h of 4Gy X-ray irradiation and Western blotting was used to determine the expression of apoptosis-related genes of 3 groups after 48 h of 4GyX-ray irradiation. Results: In the range of 25~400 pfu/cell, the inhibitory rate of A549 cell proliferation increased as adenovirus concentration raised. The inhibitory rates under the concentrations of 0, 25, 100, 200, and 400 pfu/cell were 0%, $4.23{\pm}0.34%$, $12.7{\pm}2.57%$, $22.6{\pm}3.38%$, $32.2{\pm}3.22%$, $38.7{\pm}4.09%$ and $47.8{\pm}5.58%$ and there were significantly differences among groups (P<0.05). SF in Ad-SIRT6 group was lower than Ad-null and control groups after 4~10Gy X-ray irradiation (P<0.05) and the sensitization enhancement ratio (SER) was 1.35 when compared with control group. Moreover, after 48 h of 4Gy X-ray irradiation, there appeared a significant increase in G1-phase cell proportion, upregulated expression of the level of apoptosis-promoting genes (Bax and Cleaved caspase-3), but a obvious decline in S-phase and G2-phase cell proportion and a significant decrease of the level of apoptosis-inhibiting gene (Bal-2) in the Ad-SIRT6 group (P<0.05). Conclusion: The over-expression of adenovirus-mediated SIRT6, which has radiosensitization effect on A549 cells of NSCLC, can inhibit the proliferation of A549 cells and cause G0/G1 phase retardation as well as induce apoptosis of cells.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.15 no.2
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• pp.78-86
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• 2015

Purpose: Homocystinuria (OMIM#236200) is a metabolic disease caused by mutation in the CBS gene. This study was conducted to identify the clinical features and prognosis of homocystinuria as well as to find out the CBS gene mutations of the six homocystinuria patients who were receiving treatment in the Pediatric Department at Soonchunhyang University Hospital. Methods: From January 1992 to March 2015, clinical, biochemical, and genetic analyses were performed retrospectively on the six patients diagnosed with classic homocystinuria at Soonchunhyang University Hospital. Results: A total of six patients were included in this study, including three who were diagnosed with homocystinuria at the mean age of $50{\pm}22.5$ days based on their abnormal newborn screening test results. The other three were diagnosed at the mean age of 7, when they visited the hospital for evaluation of developmental delay and lens dislocation. The group diagnosed at early infancy had normal cognitive function, but the other group had varying degrees of mental retardation. Major complications were found only in the group diagnosed after infancy. CBS gene mutation was found in all the patients, and they were all non-responsive to vitamin B6 treatment. At present, all patients' diets are controlled following a methionine-free formula and they are on medication with folic acid, betaine, pyridoxine, and methylcobalamin. Conclusion: Six homocystinuria patients were monitored for up to 23 years. The group diagnosed at early infancy exhibited no major complications. Therefore, early diagnosis is crucial in the prognosis, and homocystinuria must be included in the newborn screening program.

• The korean journal of orthodontics
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• v.30 no.6 s.83
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• pp.669-675
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• 2000

Excessive production of parathyroid hormone causes bony disorder such as periosteal bone resorption and bone pain due to excessive skeletal demineralization. A Class III facial deformity case with generalized root resorption presented bete was fumed out to be due to hyperparathyroidism. Clinical and cephalometric analysis revealed a straight skeletal profile with a retruded maxilla and a prognathic mandible. The x-ray findings demonstrated generalized root resorption of entire dentition to different degree. There also appeared osteoporosis like immature trabecular structure with the evidence of ground glass appearance. Serum test showed elevated 1evel of parathyroid hormone and growth hormone. Change of cranial growth by hyperparathyroidism can be dependent up(In a decreased bone apposition in viscerocranial growth site and abnormalities in cranial suture growth. It is possible to hypothesize that growth retardation of maxilla at least partially be accounted lot hyperparathyroidism. Therefore, regarding to the definite etiology of skeletal Class III and orthodontic treatment planning considering root resorption and osteoporosis, the early diagnosis for the hyperparathyroidism should be carefully carried by clinical and laboratory studies.

• Pediatric Infection and Vaccine
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• v.24 no.1
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• pp.65-70
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• 2017

Cytomegalovirus (CMV) infection is one of the most common congenital infections. The first case of discordant congenital CMV infection in twins occurred in Korea. A 31-year-old woman became pregnant with twins (dichorionic-diamniotic). An elective caesarean section was performed at 37 weeks. The first baby was male, weighing 2,410 g with an Apgar score of 8/9. The second baby was female, weighing 1,380 g with an Apgar score of 5/8. She had experienced intrauterine growth retardation, and presented with microcephaly, micrognathia, and joint stiffness. During the work-up for discordant twins, the second baby's serum test was positive for CMV immunoglobulin M. Her urine, blood, and cerebrospinal fluid (CSF) were CMV polymerase chain reaction positive. The first baby's CMV tests were negative. Ophthalmologic exam and audiometry performed on the second baby showed CMV retinitis and bilateral sensorineural hearing loss. She was treated with intravenous ganciclovir. Currently, she is bed-ridden and has significant developmental delay. Although the causes of discordant congenital CMV infection in twins are unclear, this case shows that discordant congenital CMV infection should be considered in twins with significant differences in intrauterine growth or clinical symptoms after birth.

• Fire Science and Engineering
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• v.27 no.5
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• pp.57-63
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• 2013

This study was performed to test the combustive properties of Pinus rigida plates treated with piperazinomethyl-bisphosphonic acid (PIPEABP), methylpiperazinomethyl-bis-phosphonic acid (MPIPEABP), and N,N-dimethylethylenediaminomethyl- bis-phosphonic acid (MDEDAP). Pinus rigida specimens were painted in three times with 15 wt% alkylenediaminoalkyl- bis-phosphonic acid solutions at the room temperature. After drying specimen treated with chemicals, combustive properties were examined by the cone calorimeter (ISO 5660-1). As a result, the combustion-retardation properties were increased by due to the treated alkylenediaminoalkyl-bis-phosphonic acid solutios in the virgin Pinus rigida. Especially, the specimens treated with chemicals showed both the later time to ignition (TTI) (148-116 s) and longer time to flameout (Tf) (633-529 s) than those of virgin plate by reducing the burnig rate. Compared with virgin pinus rigida plate, the specimens treated with the alkylenediaminoalkyl-bis-phosphonic acids showed partially low combustive properties. However the specimens treated with PIPEABP showed both the higher peak heat release rate (PHRR) (187.56 $kW/m^2$) and higher total heat release rate (THRR) (75.7 $MJ/m^2$) than those of virgin plate.

• Fire Science and Engineering
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• v.27 no.4
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• pp.21-26
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• 2013

This study was performed to test the combustive properties of Pinus rigida plates treated with bis-(dimethylaminomethyl) phosphinic acid (DMDAP), bis-(diethylaminomethyl) phosphinic acid (DEDAP), and bis-(dibuthylaminomethyl) phosphinic acid (DBDAP). Pinus rigida specimens were painted in three times with 15 wt% bis-(dialkylaminoalkyl) phosphinic acid solutions at the room temperature. After drying specimen treated with chemicals, combustive properties were examined by the cone calorimeter (ISO 5660-1). It is supposed that the combustion-retardation properties were improved by the partial due to the treated bis-(dialkylaminoalkyl) phosphinic acids in the virgin Pinus rigida. Especially, the specimens treated with chemicals showed both the lower total smoke release rate (TSRR) ($16.94{\sim}18.92m^2/m^2$) and lower $CO_{2mean}$ production (1.98~2.09 kg/kg) than those of virgin plate. However the specimens treated with chemicals showed both the higher peak mass loss rate (PMLR) (0.1250~0.1297 g/s) and higher 1st-smoke production rate (SPR) (0.0153~0.0167 g/s) than those of virgin plate. Compared with virgin Pinus rigida plate, the specimens treated with the bis-dialkylamimoalkyl phosphinic acids showed partially low combustive properties.