• 생약학회지
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• 제10권3호
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• pp.119-124
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• 1979

Both wild peony and cultivated peony demonstrated a slight effect and tranquilizer of anti-convulsion on the strychinine and picrotoxine but the wild plant was more effective. The methanol extract of cultivated peony root demonstrated a weak effect of muscle loosening but its mixture with water extractable fraction did not have this effect in contrast to the case wild type whose methanol extract mixture with water extractable fraction still retained a strong anti-convulsional muscle loosening effect. Therefore, it is concluded that wild peony root has more analgesic and muscle loosening effect than the cultivated counterpart has.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.823-834
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• 2014

In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of $50{\mu}M$ methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas $50{\mu}M$ salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of $500{\mu}M$ had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment.

• Imaging Science in Dentistry
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• 제35권4호
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• pp.199-202
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• 2005

Purpose : To report the incidence of radiological findings from screening panoramic radiographs and verify the validity of the panoramic radiography for screening purposes. Materials and Methods : Six thousand one hundred and sixty panoramic radiographs taken from the patients visiting the Health Promotion Center of CNUH were selected for this retrospective study. Panoramic radiographs were examined into the following pathologic conditions : the presence of periodontal bone loss, dental caries, periapical radiolucencies, retained roots, impacted supernumerary teeth, impacted third molars, odontoma, cystic lesions other than radicular cyst, sialoliths, and mixed radiolucent-radiopaque lesions. Number of pathologic conditions and Prevalence values were recorded. Results: The prevalences of pathologic conditions were $72.9\%$ of periodontal bone loss, $32.2\%$ of dental caries, $11.9\%$ of periapical radiolucencies, $10.8\%$ of retained roots, $0.4\%$ of root fracture, $1.0\%$ of impacted supernumerary teeth, $1.0\%$ of impacted third molars, $0.06\%$ of odontoma, $0.08\%$ of cystic lesion other than radicular cyst, $0.2\%$ of prolonged retention of deciduous tooth, $0.1\%$ of sialolith, and $0.04\%$ of mixed radiopaque and radiolucent lesion. Conclusion : Although the panoramic radiograph should not be used to replace intraoral radiographic and clinical examinations, this study showed that many dental pathologic conditions could be detected on panoramic radiographs. The panoramic radiograph might serve as a diagnostic aid in dental health evaluation programs.

• Imaging Science in Dentistry
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• 제37권2호
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• pp.83-86
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• 2007

Purpose : To evaluate the efficacy of panoramic radiography by comparing the results of clinical examination with radiographic findings. Materials and Methods: We studied 190 patients (20 men and 170 women; mean age, 40 years; range, 22 to 68 years) who visited the health promotion center of Korea Medical Science Institute and were examined both clinically and by panoramic radiography. We compared results from both examinations. Treatment options by clinical examination were described as "no treatment indicated", "treatment of dental caries", "removal of calculus", "treatment of periodontal disease", "prothodontic treatment" and "extraction of the third molar". Findings taken from the panoramic radiography were: dental caries, periapical lesion, alveolar bone loss, calculus deposition, retained root, impaction of the third molar, disease of maxillary sinus, bony change of mandibular condyle, etc. Results: The prevalence of panoramic findings were: 37.9% of dental caries, 17.4% of periapical lesions, 44.7% of alveolar bone losses, 62.6% of calculi deposition, 7.9% of retained roots, 26.8% of third molar impactions, 6.3% of diseases of maxillary sinus, 2.1 % of bony changes of mandibular condlye and 35.8% of miscellaneous lesions. Abnormal conditions revealed by panoramic radiography which had not been discovered on clinical examination were: 24.2% of the patients had dental caries, 17.4% had periapical lesions, 7.4% had calculi deposition, 5.3% had retained roots, 15.3% had third molar impactions. The opposite cases were: 5.2% had dental caries, 12.6% had calculi deposition, and 9.5% had third molar impactions. Conclusion: The use of panoramic radiography as a supplement to the clinical examination might be a valuable screening technique.

• BMB Reports
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• 제43권1호
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• pp.9-16
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• 2010

The promoters of OsCaM1 and OsCaM3 were characterized after sequencing and fused to the reporter gene, GUS. The constructs were then transformed into the tobacco plant. Histochemical analysis of GUS showed different expression patterns in pOsCaM1::GUS and pOsCaM3:: GUS transgenic plants. The expression of pOsCaM1::GUS in 4- to 15-day-old seedlings in particular was observed only in the root, while the expression of pOsCaM3::GUS was detected in both the cotyledons and root. Also, pRCaM1::GUS was detected in all the tissues surrounding the root system, while the presence of pOsCaM3::GUS was observed in the root, except in the root meristem. However, in mature transgenic plants, the expression of pOsCaM1::GUS and OsRCaM3::GUS was scarcely detected. Under wounding stress, the GUS activity of pOsCaM1 and pOsCaM3 was strongly induced, and the activity of pOsCaM3 especially, was retained for long periods. In the phloem, pOsCaM3 activity induced by hormone treatments and abiotic stresses was also identified.

• 한국식품영양과학회지
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• 제39권2호
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• pp.287-293
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• 2010

소맥분에 백합 구근분말을 첨가한 물리적 특성은 백합 구근분말 첨가 비율이 증가할수록 farinogram에서 탄력도는 증가하는 경향이었으나, 점탄성, 흡수율, 흡수 시간 및 안정도는 3%이상 첨가 시 증가 후 감소하는 경향을 보였다. Rapid viscoanalyzer(RVA)에서 유지 강도, 최종점도, breakdown, setback 값, alveogram의 $P_{max}$값과 falling number 값은 감소하였다. 소맥분에 백합 구근의 첨가량과 입도 차에 의한 호화도 특성에서 초기 호화온도는 일반 분쇄 및 미분쇄($10\;{\mu}m$) 백합 구근분말의 종류와 양에 관계없이 영향을 미치지 않았다. Rheofermentometer로 측정한 gaseous release는 전체 $CO_2$ 가스 발생량과 보유량이 감소하였다. 기호도 검사에서는 백합 구근분말의 입도와 첨가 비율에 따른 식빵의 가공적성에서 식감, 향미, 맛에서 유의적인 차이가 나타났으며, 촉감과 크럼 색상은 유의적인 차이가 없는 것으로 나타났다. 한편 식빵의 가공 적성에서 일반 분쇄 3%와 초미분쇄 3% 첨가군이 양호하였으나, 종합적인 평가에서는 대조구 다음으로 미분쇄 백합 구근분말 3% 첨가군에서 우수하였다. 이미 백합 구근의 항산화 활성 등 생리활성은 검증되어져 발표되어 있는 만큼 제빵 가공에서 조직감의 문제를 보완하고 개선한다면 건강 기능성 제빵으로 상품화 가치가 있다고 생각된다.

• 대한치과교정학회지
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• 제34권1호
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• pp.71-81
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• 2004

치아의 맹출 과정과 치간이개로 유도된 치아 및 치조골의 흡수 과정에서 치주인대 세포와 치주인대 단백질의 기능을 알아보기 위하여, 발육 중인 흰쥐를 치근 형성 전, 치근 형성 시작과 치근 형성 및 맹출 시기로 구분하여 조직 표본을 제작하고, 또한 성 장 중인 흰쥐를 2주간 치간 이개시켜 조직표본을 제작하였다. 치주인대 섬유모세포에서 특이적으로 발현되며 치주인대의 분화와 성숙에 관여하는 PDLs22단백질과 치아와 치조골의 파괴와 흡수를 조절하는 것으로 알려진 RANKL과 OPG의 발현을 면역 조직화학적으로 연구하였다. PDLs22 단백질은 치근 형성이 시작되면서부터 치낭세포와 골모세포에서 발현되어, 치아가 맹출하는 과정에서도 그 발현이 계속 유지되었으나, 치간이개에 의하여 치주인대가 개조되는 부위에서는 발현이 감소하였다. RANKL은 치근형성 과정에서는 미약한 발현을 나타내었으나, 치아가 맹출하면서 발현이 증대되었으며, 치간이개에 의한 치근과 치조골 흡수과정에서는 치주인대세포, 골모세포, 치수세포 및 파치세포에서 발현이 증대되었다. OPG는 치근이 형성되는 시기에는 강한 발현을 보였으나, 치아가 맹출하면서 발현이 현저히 감소하였고, 치아와 치조골의 흡수가 진행됨에 따라서 발현이 다소 감소하였다.

• Restorative Dentistry and Endodontics
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• 제39권3호
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• pp.201-209
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• 2014

Objectives: This study evaluated the solubility, dimensional alteration, pH, electrical conductivity, and radiopacity of root perforation sealer materials. Materials and Methods: For the pH test, the samples were immersed in distilled water for different periods of time. Then, the samples were retained in plastic recipients, and the electrical conductivity of the solution was measured. The solubility, dimensional alteration, and radiopacity properties were evaluated according to Specification No. 57 of the American National Standards Institute/American Dental Association (ANSI/ADA). Statistical analyses were carried out using analysis of variance (ANOVA) and Tukey's test at a significance level of 5%. When the sample distribution was not normal, a nonparametric ANOVA was performed with a Kruskal-Wallis test (${\alpha}$ = 0.05). Results: The results showed that white structural Portland cement (PC) had the highest solubility, while mineral trioxide aggregate (MTA)-based cements, ProRoot MTA (Dentsply-Tulsa Dental) and MTA BIO ($\hat{A}$ngelus Ind. Prod.), had the lowest values. MTA BIO showed the lowest dimensional alteration values and white PC presented the highest values. No differences among the tested materials were observed in the the pH and electrical conductivity analyses. Only the MTA-based cements met the ANSI/ADA recommendations regarding radiopacity, overcoming the three steps of the aluminum step wedge. Conclusions: On the basis of these results, we concluded that the values of solubility and dimensional alteration of the materials were in accordance with the ANSI/ADA specifications. PCs did not fulfill the ANSI/ADA requirements regarding radiopacity. No differences were observed among the materials with respect to the pH and electrical conductivity analyses.

• 대한소아치과학회지
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• 제34권1호
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• pp.156-161
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• 2007

소아치과 임상에서는 외상성 손상에 의하거나 발치 도중 우발적으로 유치의 치근이 파절되는 경우가 종종 있다. 이런 경우 임상의들은 치근 파절편을 발치할지, 남겨둘지에 대해 간혹 고민하게 된다. 이같은 상황에서 잔근이나 파절편의 발치 시도가 계승치 배를 손상시킬 우려가 있을 경우에는 일반적으로 치근 파절편을 남겨두는 것이 추천되고 있다. 본 연구는 유치 잔근이나 파절편을 술자 의도적으로 잔존시킨 경우, 계승치의 맹출경로에 장애를 가하지 않고 소멸될 것인지의 예후를 확인해 볼 목적으로 잔근이나 치근 파절편을 의도적으로 남긴 3세 8개월${\sim}6$세 1개월 어린이 6명을 대상으로 $7{\sim}37$개월 동안 $2{\sim}3$개월 주기의 내원을 통하여 임상검사와 방사선검사를 시행하고 예후를 장기 관찰하였다. 관찰 결과, 5 증례에서 치근측 파절편은 생리적인 흡수과정을 통하여 크기가 축소되거나 소멸되었고, 1 증례에서는 계승치와 함께 밀려 출은하였으며 계승치의 맹출을 현저하게 지장을 초래한 경우는 나타나지 않았다. 본 관찰을 통하여 치조골 내에 남은 유치근 파절편이 계승치배에 근접한 경우, 이를 무리하게 발치하려 시도하기보다는 치배의 손상 가능성을 고려하여 잔존시키고 주기적인 검진을 통해 흡수 여부를 판단하며 예후 관리를 하는 방법의 타당성을 확인할 수 있었다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2000년도 추계학술대회
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• pp.182-195
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• 2000

Indigenous bacteria from poplar roots (Populus mnadensis var. eugenei, 'Imperial Carolina') and Southern Californian shrub rhizospheres as well as two tree-colonizing Rhizobium strains (ATCC 10320 and 35645) were genetically engineered to express constitutively and stably toluene o-monooxygenase (TOM) from Burkholderia cepacia G4 by integrating the torn locus into the chromosome. The poplar and Rhizobium recombinants degraded trichloroethylene (TCE) at 0.8-2.1 nmol/min.mg protein (initial TCE concentration, 10u M) and competitive against the unengineered hosts in wheat and barley rhizospheres for one month (colonization at 1-23 $\times$ 10$^{5}$ CFU/cm root). In addition, six of these recombinants colonized poplar roots stably and competitively with populations as high as 79 $\pm$ 12% of all rhizosphere bacteria after 28 days (0.2-31 $\times$ 10$^{5}$ CFU/cm root). Furthermore, five of the most-competitive poplar recombinants (e.g., Pb3-1 and Pb5-1 which were identified as Pseudomonas PsK) retained the ability to express TOM for 29 days as 100 $\pm$ 0% of the recombinants detected in the poplar rhizosphere had constitutive expression of TOM.