• Water for future
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• v.6 no.2
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• pp.19-29
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• 1973

The progressive contamination of water resulted from man's activity and the use of fertilizers is not restricted only to surface water, but also the shallow groundwater is affected. This type of groundwater contamination is mainly restricted to areas composed of permeable, nonconsolidated sediments forming a shallow aquifer. The chloride and the sulfate resulted from man's activity and the use of fertilizers were measured to study the variations of the groundwater contamination. In general, (1) When water level rises, the rate of groundwater contamination becomes less and when water level declines, the rate of contamination is increased. (2) The highly contaminated season is the early-summer and the less contaminated season is the winter or after rainy season. (3) The groundwater in weathering zone without covering layer. (4) The degree of contamination of wells is increased with the increase of well depth and lowing the water table, because of increasing contaminated water from enlargement of the area of influence of the well.

• IMMUNE NETWORK
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• v.10 no.4
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• pp.135-143
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• 2010

Background: Cordyceps militarys water extract (CME) has been reported to exert antitumor and immunomodulatory activities in vivo and in vitro. However, the therapeutic mechanism has not yet been elucidated. In this study, we examined the effects of CME on the antigen presenting function of antigen presenting cells (APCs). Methods: Dendritic cells (DCs) were cultured in the presence of CME, and then allowed to phagocytose microspheres containing ovalbumin (OVA). After washing and fixing the efficacy of OVA, peptide presentation by DCs were evaluated using CD8 and CD4 T cells. Also, we confirmed the protein levels of proinflammatory cytokines through western blot analysis. Results: CME enhanced both MHC class I and class II-restricted presentation of OVA in DCs. In addition, the expression of both MHC class I and II molecules was enhanced, but there was no changes in the phagocytic activity of exogenous OVA. Furthermore, CME induced the protein levels of iNOS, COX-2, proinflammatory cytokines, and nuclear p65 in a concentration-dependent manner, as determined by western blot. Conclusion: These results provide an understanding of the mechanism of the immuno-enhancing activity of CME on the induction of MHC-restricted antigen presentation in relation to their actions on APCs.

• IMMUNE NETWORK
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• v.5 no.3
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• pp.150-156
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• 2005

Background: Dendritic cells (DCs) playa critical role not only in the initiation of immune responses, but also in the induction of immune tolerance. In an effort to regulate immune responses through the modulation of antigen presenting cell (APC) function of DCs, we searched for and characterized APC function modulators from natural products. Methods: DCs were cultured in the presence of propolis components, WP and CP, and then examined for their ability to present exogenous antigen in association with major histocompatibility complexes (MHC). Results: WP and CP inhibited class I MHC-restricted presentation of exogenous antigen (cross-presentation) in a DC cell line, DC2.4 cells, and DCs generated from bone marrow cells with GM-CSF and IL-4. The inhibitory activity of WP and CP appeared to be due not only to inhibition of phagocytic activity of DCs, but also to suppression of expression of MHC molecules on DCs. We also examined the effects of WP and CP on T cells. Interestingly, WP and CP increased IL-2 production from T cells. Conclusion: These results demonstrate that WP and CP inhibit MHC-restricted presentation of exogenous antigen through down-regulation of phagocytic activity and suppression of expression of MHC molecules on DCs.

• IMMUNE NETWORK
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• v.16 no.4
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• pp.233-241
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• 2016

DCs, like the sensory neurons, express vanilloid receptor 1 (VR1). Here we demonstrate that the VR1 agonists, capsaicin (CP) and resiniferatoxin (RTX), enhance antiviral CTL responses by increasing MHC class I-restricted viral antigen presentation in dendritic cells (DCs). Bone marrow-derived DCs (BM-DCs) were infected with a recombinant vaccinia virus (VV) expressing OVA (VV-OVA), and then treated with CP or RTX. Both CP and RTX increased MHC class I-restricted presentation of virus-encoded endogenous OVA in BM-DCs. Oral administration of CP or RTX significantly increased MHC class I-restricted OVA presentation by splenic and lymph node DCs in VV-OVA-infected mice, as assessed by directly measuring OVA peptide SIINFEKL-K^b complexes on the cell surface and by performing functional assays using OVA-specific CD8 T cells. Accordingly, oral administration of CP or RTX elicited potent OVA-specific CTL activity in VV-OVA-infected mice. The results from this study demonstrate that VR1 agonists enhance anti-viral CTL responses, as well as a neuro-immune connection in anti-viral immune responses.

• Proceedings of the Society of Korea Industrial and System Engineering Conference
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• 2002.05a
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• pp.69-78
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• 2002

This paper examines the effects on mental fatigue of changes in the intensity of physical activity. A treadmill-equipped instrument and perception tester were used to attain several levels of physical activity In this paper, In order to determine the individual levels of physical activity of subjects, Borg-RPE scale, Heart Rate(HR) and Respiratory Quotient(RQ) were used. Also, an addition test in whole-body activity on treadmill-equipped instrument as an indicator of mental fatigue were performed. In the above experiments, the scores obtained in addition test administered before and after physical activity at each intensity level used. Restricted within the limits of this paper, the results of these tests showed that mental fatigue decreased after physical activity.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.25 no.6
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• pp.42-47
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• 2002

This paper examines the effects on mental task of changes in the intensity of physical activity. A treadmill-equipped instrument and perception tester were used to attain several levels of physical activity. In this paper, in order to determine the individual levels of physical activity of subjects, Borg-RPE scale, heart rate(HR) and respiratory quotient(RQ) were used. Also, an arithmetic addition test in whole-body activity on treadmill-equipped instrument as an indicator of mental task were performed. In the above experiments, the scores obtained in arithmetic addition test administered before and after physical activity at each intensity level used. Restricted within the limits of this paper, the results of these tests showed that the performance of mental task was Increased after physical activity.

• Journal of the Korean Society of Safety
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• v.16 no.4
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• pp.182-187
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• 2001

This paper examines the effects on human reaction time and mental fatigue of changes in the intensity of physical activity. A treadmill-equipped instrument and perception tester were used to attain several levels of physical activity. In this paper, in order to determine the individual levels of physical activity of subjects, Borg-RPE scale, Heart Rate(HR) and Respiratory Quotient(RQ) were used. Also, a reaction test in whole-body activity on treadmill-equipped instrument and an addition test as an indicator of mental fatigue were performed. In the above experiments, the scores obtained in addition test administered before and after Physical activity at each intensity level used. Restricted within the limits of this paper, the results of these tests showed that mental fatigue decreased after physical activity.

• Archives of Pharmacal Research
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• v.29 no.12
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• pp.1147-1153
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• 2006

Mizoribine (MZR) has been shown to possess immunosuppressive activity that selectively inhibits the proliferation of lymphocytes by interfering with inosine monophosphate dehydrogenase. The efficacy of MZR is not only in patients who have had renal transplantation, but also in patients with rheumatoid arthritis (RA), lupus nephritis, and primary nephritic syndrome. Because the exact mechanism of its immunosuppressive action is not clear, the object of this study was to examine the ability of MZR to regulate the antigen presenting cells (APCs), dendritic cells (DCs). In this work, we tested whether MZR ($1{\sim}10\;{\mu}g/mL$) could inhibit the cross-presentation of DCs. DC2.4 cells ($H-2K^{b}$) or bone marrow-derived DCs (BM-DCs) generated from BM cells of C57BL/6 mouse ($H-2K^{b}$) were cultured in the presence of MZR with OVA-microspheres, and the amount of OVA peptide-class I MHC complexes was measured by a T cell hybridoma, B3Z, that recognizes OVA (257-264 : SIINFEKL)-$H-2K^{b}$ complex and expresses-galactosidase. MZR profoundly inhibited the expression of SIINFEKL-$H-2K^{b}$ complexes. This inhibitory activity of MZR appeared to affect the phagocytic activity of DCs. MZR also decreased IL-2 production when we examined the effects of MZR on $CD4^{+}$ T cells. These results provide an understanding of the mechanism of immunosuppressive activity of MZR on the inhibition of MHC-restricted antigen presentation and phagocytic activity in relation to their actions on APCs.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2005.10a
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• pp.143-146
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• 2005

Human activity pattern has been changed as the contemporary urban society changes. Diverse activities repeat regular patterns as time passes. Diagram is a simple drawing which aims to organize and unify various information. The elements of the social behaviour could be spatialized by means of diagram applications. By using diagrams, architects understand contemporary urban society and form new space conditions. Time-based activity patterns consists of activity pattern in a restricted space and in urban structure for space use. Activity patterns for different time zones are explained by two types of diagrams, space occupation and flexibility of space, By the characteristic of space system structred by these diagrams, activities and programs are rearranged and variety of space is allowed through flexibility. Also, programs are mixed to apply to simultaneous occurrence of ever-changing human activities.

• Journal of Periodontal and Implant Science
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• v.26 no.3
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• pp.689-699
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• 1996

The purpose of this research is to determine elements affecting the out-of-pocket cost of woman. The sample consisted of 1907 women living Iksan city. The survey was conducted by means of questionnaires. The model used in the analysis of out-of-pocket cost was the Andersen-Newman model, while the analysis techniques used were stepwise multiple regression and path analysis. The number of independent variables used in the analysis was 28 in total, ie 19 predisposing components, 6 enabling components, and 3 need components. In this study, the amount of variance by the model was 17 percent. Number of restricted activity days caused by oral disease, perceived susceptibility of dental disease, having a regular dental care, dental treatment costs, education level and income were found to have significant major effects on out-of-pocket cost. Number of restricted activity days caused by oral disease was the most important variable affecting out-of-pocket cost of woman. Also out-of-pocket cost shows larger effect due to enabling components than frequency of dental utilization.