• Title/Summary/Keyword: response-surface

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Gypsy moth kairomones affecting host acceptance behavior of the egg parasitoids Ooencyrtus kuvanae (Hymenoptera:Encyrtidae) (짚시알 깡충좀벌(벌목:깡충좀벌과)의 기주수락행동에 영향을 미치는 매미나방의 카이로몬)

  • 이해풍;부경생;김수언;이기상
    • Korean journal of applied entomology
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    • v.36 no.1
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    • pp.88-95
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    • 1997
  • Olfactometer was used to find the attractiveness of kairomone extracted from gypsy moth (Lymantria dispar) to the egg parasitoid (Ooencyrtus kuvanae). The attractiveness of kairomone extracted from scales and hairs which were attached to the surface of the eggs were higher than that of whole eggs with 79.9% and 67.6% respectively. The probing lantency of the egg parasitoid on the extract of scales and hairs were 115.5 sec. and that of whole eggs were 113.2 sec. Eggs taken from egg mass were shown 91.0% of attractiveness to the egg parasitoid, however only 15% of egg parasitoid were attracted by follicles dissected from the host ovary. The probing latency of egg mass was faster than that of follicles dissected from the host with 84.2 sec. and 114.0 sec. respectively. Egg parasitoid showed the most active attractiveness (88.0%), relatively longer examing period (89.2 sec.) and shorter probing latency (26.8 sec.) to the adhesive materials secreted from reproductive accessory glands of the host. Out of six organic solvents used for the extraction of kairomone from the host egg mass, n-hexane fraction received the most active response from xthe egg parasitoid and the parasitoid reared in vivo showed 3 times higher attractiveness to the n-hexane fraction than that of in vitro.

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Influence of lossy ground on impulse propagation in time domain for impulse ground penetrating radar (초광대역 임펄스 지반탐사레이더에서 지면의 영향에 따른 임펄스 전파 특성 연구)

  • Kim, Kwan-Ho;Park, Young-Jin;Yoon, Young-Joong
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.44 no.11
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    • pp.42-47
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    • 2007
  • In this paper, influence of lossy ground and gap variation between lossy ground and UWB antenna on impulse propagation in time domain for impulse ground penetrating radar (GPR) is numerically and experimentally investigated. For this study, a novel planar UWB fat dipole antenna is developed. First, influence of lossy ground and gap variation between lossy ground and UWB antenna is simulated. For verification, a test field of sand and wet clay soil is built and using the developed dipole antenna, transmission behavior is investigated at the test field. With an aid of IDFT (inverse discrete Fourier transform), time domain impulse response for transmission coefficient measured and simulated in frequency domain is obtained. Measurement and simulation show that the frequency of maximum transmission coefficient and transmission coefficient are increased with higher dielectric constant and larger gap distance. In time domain, it is shown that for higher dielectric constant, the amplitude of the received signal in time domain is higher and reflected signals are seriously modified. Also, it is found that variation of gap between antenna and ground surface makes timing of peak value changed.

Optimization of Blanching Process of Cirsium setidens and Influence of Blanching on Antioxidant Capacity (참취 데치기 최적 공정 확립과 추출물의 항산화 특성 변화)

  • Jo, Hyeon Seon;Ha, Yoo Jin;Kim, Yeon Tae;Kang, Gil Nam;Yoo, Sun Kyun
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.1
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    • pp.173-182
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    • 2017
  • This study was conducted to investigate the change of Aaster scaber antioxidative activity, total phenolic compounds and flavonoids during the blanching processes. With two parameters such as blanching time and temperature, response surface methodology and central composite design was used to study the combined effect of blanching time (90 to 162 sec) and blanching temperature (75 to $99^{\circ}C$). We found that antioxidative activity, total phenolic compounds and flavonoids during the blanching processes were influenced by blanching temperature and time. Within process condition, total phenolic compounds and flavonoids were extracted 3.00 - 35.48 mg/g and 2.35 - 8.38 mg/g, respectively. DPPH radical scavenging activity was 42.10 - 67.14%. The change of total phenolic compounds, flavonoids, and DPPH radical scavenging activity was dependent of blanching temperature more than time. The total phenolic compounds was increased as temperature rise, but flavonoids not. However, DPPH radical scavenging activity was increased during the blanching process.

Process Optimization of Peptides Production from Protein of Sea Cucumber and Its Antioxidant Capacity Analysis (해삼 단백질로부터 펩타이드 제조 최적공정 확립 및 항산화 특성)

  • Ha, Yoo Jin;Yoo, Sun Kyun
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.2
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    • pp.338-348
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    • 2017
  • Protein hydrolysates derived from plants and animals having antioxidant, suppression of hypertension, immunodulatory, alleviation of pain, and antimicrobial activity has been known as playing important role like hormone. This study was performed to optimize the hydrolysis of protein of sea of cucumber by a flavourzyme. The ranges of processes were the reaction temperature of 40 to $60^{\circ}C$, pH 6 to 8, and enzyme concentration 0.5 to 1.5%(w/v). As a result, the optimization of process was determined at temperature of $48-50^{\circ}C$, pH of 7.0-7.2, and enzyme concentration of 1.0-1.1%(w/v), and degree of hydrolysis was 43-45 at above conditions. The molecular weight of hydrolysate was distributed to 500-3,500 Da and showed typical peptides. Inhibition concentration ($IC_{50}$) of peptides of DPPH radical scavenging activity, Superoxide anion radical scavenging activity, Hydroxy radical scavenging activity, $Fe^{2+}$ cheating activity was 1.25, 3.40, 10.3, and 22.11 mg/mL, respectively. Therefore, we expect that those products are useful as functional food ingredients.

Optimization of Peptides Production Derived from By-product Viscera of Yoensan Ogae Meat Process (연산 오계 부산물 내장육으로부터 펩타이드 생산 최적 공정 개발과 생산물의 특성 분석)

  • Ji, Joong Gu;Yoo, Sun Kyun
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.3
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    • pp.549-559
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    • 2016
  • Korean Black body fowl (Gallus gallus domesticus; Ogae) designated as a natural monument (registration number 265) has been known as a superb traditional Korean medicine. In this study, The production of peptide from the Viscera Waste of Yeonsan Ogae was optimized using commercial protease (bromelain) by response surface methodology under high pressure process. The range of processes was pressure (30 to 100 MPa), reaction time (1 to 5 h), and substrate concentration (10 to 30%, w/v). After reaction, the degree of hydrolysis, distribution of amino acids, and molecular weight of peptides were investigated. As a results, the optimization conditions were pressure 90 MPa, reaction time 3 to 4 h, and the amount of viscera meat 20% (w/v), respectively. The molecular weight of protein hydrolysates was distributed 400 to 1,000 Da. Accordingly we presumed that most products were peptides. Of those peptides, nonpolar or hydrophobic, polar but uncharged, positively charged, and negatively charged amino acids were 42.03, 26.0, 13.3, and 18.6%, respectively. Because higher amount of hydrophobic amino acids, we expected that those products would be able to utilize as the functional food ingredients.

Relationship between fatigue resistance and fracture behavior of the carbon fiber sheet and carbon fiber strand sheet reinforced RC slabs (Carbon fiber sheet 및 carbon fiber strand sheet 접착보강한 RC 상판의 내피로성과 파괴거동과의 상관관계)

  • Won, Chan Ho;Abe, Tadashi;Ahn, Tae-Ho;Kim, Do Keun
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.6
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    • pp.294-298
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    • 2015
  • According to the results of "Highway Bridges Long Life Repair Plan." The most serious damage to RC slabs is caused by fatigue deterioration, which results from the driving loads of large-sized vehicles, and aging of materials. In response to this, adhesion reinforcement using carbon fiber sheet is being adopted. In addition, carbon fiber strand sheet that holds the same material characteristics as CFS, but has superior workability, has been developed as a new reinforcement material. However, almost no studies have been conducted on CFSS in relation to fatigue resistance evaluation through fatigue tests under running wheel loads, with the exception of a few by some organizations. Therefore, in this study, specimens with front CFS adhesion reinforcement on the bottom surface of the RC slab and specimens with grid-type CFSS reinforcement were manufactured. Then, fatigue tests under running wheel loads were conducted, and thus fatigue resistance was evaluated using the specimens.

Hydrolysis of Isolate Soybean Protein Using Subcritical Water (아임계수를 이용한 분리대두단백질의 가수분해)

  • Hwang, Yun Hee;Cho, Hyung-Yong;Kim, Ko-Rae;Lee, Seok Hoon;Choi, Mi-Jung;Shin, Jung-Kue
    • Korean Journal of Food Science and Technology
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    • v.47 no.6
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    • pp.772-778
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    • 2015
  • Hydrolysis of isolate soybean protein (ISP) using subcritical water (SCW) was conducted to study the feasibility for producing protein hydrolyzate. SCW hydrolysis of SPI suspension (5-15%) was conducted in an electrically heated batch reactor (2 L). The effects of temperature (230 to $270^{\circ}C$) and holding time (10 to 50 min) on the degree of hydrolysis (DH) and the production of amino acids were studied by surface response method. The DH was determined by derivatizing the hydrolyzates with ortho-phthalaldehyde (OPA) solution. It was confirmed that reaction temperature and holding time affected the hydrothermolysis of soybean protein. However, the holding time was less effective on amino acid yield when the temperature was higher than $230^{\circ}C$. In order to achieve optimal yields of amino acids exceeding 43%, the temperature should be within the range between 256 and $268^{\circ}C$ with holding time from 29 to 41 min, respectively. A maximum estimated amino acid yield of 43.5% was obtained at $268^{\circ}C$ for 35 min.

Human Dermal Risk Assessment on Chlorpyrifos of Korean Farmers (우리나라 농민의 Chlorpyrifos에 대한 피부 위해성 평가)

  • 정경미;이효민;이은희;이선희;김진화;심영용;홍진태;이용욱
    • Environmental Mutagens and Carcinogens
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    • v.22 no.3
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    • pp.187-198
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    • 2002
  • Chlorpyrifos is an organophosphate insecticide and one of the most commonly and widely used insecticide. However, a little known about the dermal risk of chlorpyrifos on human being. Therefore, this study was conducted for the dermal risk assessment after exposure to chlorpyrifos in Korean farmers. First, skin irritation by chlorpyrifos (10 mg/$\textrm{cm}^2$, 50 mg/$\textrm{cm}^2$, 100 mg/$\textrm{cm}^2$, 250 mg/$\textrm{cm}^2$ in acetone) was determined in rabbits for 5 days considering the usage of chlorpyrifos short term highly exposure. The index of skin irritation by chlorpyrifos was increased in each dose and length of exposure dependent manners. Next, using benchmark dose (BMD$_{5}$) approach, the dose-response relationship was assessed to calculate the reference dose (RfD). The value of RfD was 2.84 $\mu\textrm{g}$/kg/day from 142.16 $\mu\textrm{g}$/kg/day BMD5 value divided uncertainty factor 50. Finally, we assessed human dermal risk of chlorpyrifos with exposure level and RfD. Skin absorbed levels were assumed with several exposure scenarios encounting the circumstances of exposure that application method, protection equipment and cloth, exposure time and exposure frequency during chlorpyrifos spraying. By the comparison of skin absorbed dose with the reference dose, it was identified that risk values (risk index) to skin chlorpyrifos exposure were 0.958 from the point of above results and it was recommended that the occurrence of hazard effect (skin irritation toxicity) of chlorpyrifos would not be expected. Risk index was smaller than 1 in the case of spraying vehicle mounted application, 1hour exposure time and wearing protective cloth exposure. Whereas, risk index was above 1 in the case of hand-held application, 2hour exposure time and wearing common cloth. Comparing two kinds of application method, total risk index of the hand held application (1.67) was higher than vehicle mounted (0.27). Therefore, chlorpyrifos skin exposure was mainly affected by application equipment and applied form. The results of risk assessment on the human dermal toxicity of chlorpyrifos should be required to control in keeping safety rules, skin surface area available for contact, spraying time ,and spraying frequency.y.

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Influence of Substrate Concentration and Hydraulic Retention Time on the Hydrogen Production Using Anaerobic Microflora (혐기성 미생물을 이용한 수소생산에 있어서 기질농도 및 수리학적 체류시간의 영향)

  • Ko, In-Beom;Shin, Hang-Sik;Lee, Yong-Doo
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.9
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    • pp.911-916
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    • 2006
  • The influence of substrate concentration and hydraulic retention time(HRT) on the hydrogen production by anaerobic microflora was investigated by conducting three series of continuous experiments the individual influences of substrate concentration and HRT. In series I, substrate concentration was increased from 3 to 27 g-glucose/L keeping HRT at 8 hr. Series II and III carried out same condition with series I at HRT of 16 hr and 24 hr, respectively. The effects of HRT and substrate concentration on the hydrogen production yield were analyzed by quadratic model. The maximum hydrogen production yield of 2.05 mol $H_2/mol$ glucose was found at the HRT of 9.6 hr and the substrate concentration of 15.4 g/L. The relationship between HRT and substrate concentration on hydrogen production yield as displayed a saddle shape in the response surface plot. Optimum HRT and substrate concentration are observed at in the range of 5 and 14 hr, at between 13 and 17 g/L, respectively, for the hydrogen production yield being 2 mol $H_2/mol$ glucose. The concentrations of organic acids increased with the increase of the amount of glucose consumption. Acetic acid and butyric acid were the main by-products from the glucose degradation.

Elimination of Heavy Metals(Pb, Cd) by Steaming and Roasting Conditions of Polygonatum odoratum Roots (둥굴레 근경의 증자 및 볶음조건에 따른 중금속(Pb, Cd) 제거 특성)

  • Kim Kyung-Tae;Noh Jungeun;Lee Jungeun;Kim Jung-Ok;Lee Gee-Dong;Kwon Joong-Ho
    • Food Science and Preservation
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    • v.12 no.3
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    • pp.209-215
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    • 2005
  • Response surface methodology(RSM) was applied to monitor the elimination rate of heavy metals(Pb, Cd) and soluble solids depending on the steaming and roasting conditions of Polygonatum odoratum roots. Experiments of 16 different steaming and roasting conditions based on a central composite design for steaming time($60{\sim}180$ min), roasting temperature($110{\sim}150^{\circ}C$), and roasting time($10{\sim}50$ min) were conducted, thereby predicting the steaming and roasting conditions for the maximal responses; soluble solids($71.47\%$) at 65.24 min, $126.93^{\circ}C$ and 37.58 min; Pb removal rate($18.87\%$) at 71.23 min, $119.81^{\circ}C$ and 24.35 min; Cd removal rate($50,85\%$) at 160.89 min, $126.43^{\circ}C$ and 15.81 min, respectively. The optimum conditions estimated by RSM for the maximized values of soluble solids and heavy metal elimination rates were $165{\sim}180$ min of steaming time, $120{\sim}135^{\circ}C$ of roasting temperature, and $30{\sim}45$ min of roasting time, respectively. These estimated values were in agreement with those measured by real experiments.