• Title/Summary/Keyword: resistance detection

검색결과 555건 처리시간 0.035초

자기진단 CPGFRP의 파괴예측기능 평가를 위한 콘크리트 적용실험 (Evaluation of Fracture Detection Function for the Concrete by Self-Diagnosis CPGFRP)

  • 최현수;박진섭;정민수;강병희
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2003년도 학술.기술논문 발표회
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    • pp.27-31
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    • 2003
  • To maintain serviceability of concrete structure more than proper it is necessary not only predict service life through periodical monitor but also need monitoring system to recognize optimal time and method for repair. Recently, CPGFRP, replacing some GFRP with CF, is developed and used for monitoring concrete fraction. But dramatic resistance change of CPGFRP is showed below 0.5% strain and it is not small strain in terms of monitoring micro crack in concrete. In other word, monitoring with CF is not suitable in low stress hut hight stress. In this study, we accessed applicable possibility and reliability of CPGFRP composite as monitoring sense that is proved very sensitive to stress through domestic and oversea previous study. CPGFRP composite plays a role in specimen like steel and increases flexural strength. CPGFRP composite shows resistance increasement in micro crack. In particular, CPUFRP is more sensitive than strangage in low stress. Resistance change ratio curve is very similar to strain curve so sensitivity and reliability is very excellent to monitor concrete fracture.

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자기진단 CPGFRP의 파괴예측기능 평가를 위한 콘크리트 적용실험 (Evaluation of Fracture Detection Function for the Concrete by Self-Diagnosis CPGFRP)

  • 최현수;박진섭;정민수;강병희
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2003년도 학술.기술논문발표회
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    • pp.27-31
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    • 2003
  • To maintain serviceability of concrete structure more than proper it is necessary not only predict service life through periodical monitor but also need monitoring system to recognize optimal time and method for repair. Recently, CPGFRP, replacing some GFRP with CF, is developed and used for monitoring concrete fraction. But dramatic resistance change of CPGFRP is showed below 0.5% strain and it is not small strain in terms of monitoring micro crack in concrete. In other word, monitoring with CF is not suitable in low stress but hight stress. In this study, we accessed applicable possibility and reliability of CPGFRP composite as monitoring sense that is proved very sensitive to stress through domestic and oversea previous study. CPGFRP composite plays a role in specimen like steel and increases flexural strength. CPGFRP composite shows resistance increasement in micro crack. In particular, CPGFRP is more sensitive than strangage in low stress. Resistance change ratio curve is very similar to strain curve so sensitivity and reliability is very excellent to monitor concrete fracture.

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Development of TaqMan Probe-Based Real-Time PCR Method for erm(A), erm(B), and erm(C), Rapid Detection of Macrolide-Lincosamide-Streptogramin B Resistance Genes, from Clinical Isolates

  • Jung, Jae-Hyuk;Yoon, Eun-Jeong;Choi, Eung-Chil;Choi, Sung-Sook
    • Journal of Microbiology and Biotechnology
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    • 제19권11호
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    • pp.1464-1469
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    • 2009
  • To achieve more accurate and rapid detection of macrolide-lincosamide-streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.

전기저항 측정기법을 이용한 오염물질 누출감지시스템의 개발: I. 오염물질에 의한 지반의 전기적 특성 변화 (Development of Contaminant Leakage Detection System Using Electrical Resistance Measurement: I. Variations of Electrical Properties of Subsurface due to Contaminants)

  • 오명학;박준범;김영진;홍성완;이용훈
    • 한국지반공학회논문집
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    • 제17권6호
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    • pp.215-224
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    • 2001
  • 본 논문에서는 오염물질에 의해서 지반의 전기적 특성이 변한다는 원리에 의한 매립지의 침출수나 지하저장탱크의 유류 누출을 조기에 감지하는 시스템의 개발가능성을 확인하고자 하였다. 이를 위해 오염물질이 누출되는 상황을 모사한 실내실험을 통하여 전기저항 측정에 의한 오염물질 누출감지 가능성을 평가하였다. 불포화사질토 지반의 전기 저항 측정값은 함수비가 증가함에 따라 지수적으로 감소하는 경향을 보였다. 오염물질이 지반에 주입될 경우에 디젤은 비전도성 물질로 지반의 전기저항을 크게 증가시켰으며, NaCl 용액과 침출수는 전기전도성이 좋기 때문에 지반의 전기저항을 감소시켰다. 오염물질의 누출에 의한 전기저항은 측정전극간격에 따라 감지민감도가 다르게 나타났으며, 디젤의 경우에는 전극간격이 좁을수록, NaCl 용액과 침출수의 경우에는 전극간격이 높을수록 감지민감도가 크게 나타났다.

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Single Crystalline CoFe/MgO Tunnel Contact on Nondegenerate Ge with a Proper Resistance-Area Product for Efficient Spin Injection and Detection

  • Jeon, Kun-Rok;Min, Byoung-Chul;Lee, Hun-Sung;Shin, Il-Jae;Park, Chang-Yup;Shin, Sung-Chul
    • 한국자기학회:학술대회 개요집
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    • 한국자기학회 2010년도 임시총회 및 하계학술연구발표회
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    • pp.96-96
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    • 2010
  • We report the proper resistance-area products in the single crystalline bcc CoFe/MgO tunnel contact on nondegenerate n-Ge desirable for efficient spin injection and detection at room temperature. The electric properties of the crystalline CoFe(5 nm)/MgO(1.5,2.0,2.5 nm)/n-Ge(001) tunnel contacts have been investigated by I-V-T and C-V measurements. Interestingly, the tunnel contact with the 2-nm MgO exhibits the ohmic behavior with low resistance-area products, satisfying the theoretical conditions required for significant spin injection and detection. This result is ascribed to the presence of MgO layer between CoFe and n-Ge, enhancing the Schottky pinning parameter as well as shifting the charge neutrality level.

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Characterization of Multidrug-resistant Salmonella enterica Serovar Typhimurium Isolated from Swine Sources

  • Suh Dong Kyun;Song Jae Chan
    • 대한의생명과학회지
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    • 제11권2호
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    • pp.115-119
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    • 2005
  • A total of 28 Salmonella enterica serovar Typhimurium isolated from diseased pigs and swine carcasses between 2001 and 2003 were characterized by the antimicrobial resistance profiles, PCR for detection of S. Typhimurium DT104 and pulsed-field gel electrophoresis (PFGE) with the restriction enzyme XbaI. All but one isolate presented multidrug resistance (MDR) to more than two antibiotics tested. A total of 11 resistance profiles were observed, and two phenotypes, ST and ASSuTG, were the most common among them. Two isolates were found to be S. Typhimurium DT104 isolates by PCR, and their resistance profile did not show the DT104 typical resistance type ACSSuT, but ACSSuTGK instead. PFGE identified 11 banding patterns in dendrogram, and three main clusters (designated A to C) were represented. Interestingly, sixteen of 19S. Typhimurium isolates belonging to cluster B showed an identical band pattern.

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Early Detection of Intravenous Infiltration Using Multi-frequency Bioelectrical Impedance Parameters: Pilot Study

  • Kim, Jae-Hyung;Shin, Beum-Joo;Baik, Seung-Wan;Jeon, Gye-Rok
    • 센서학회지
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    • 제26권1호
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    • pp.15-23
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    • 2017
  • In this study, bioelectrical impedance analysis, which has been used to assess an alteration in intracellular fluid (ICF) of the body, was applied to detect intravenous infiltration. The experimental results are described as follows. Firstly, when infiltration occurred, the resistance gradually decreased with time and frequency i.e., the resistance decreased with increasing time, proportional to the amount of infiltrated intravenous (IV) solution. At each frequency, the resistance gradually decreased with time, indicating the IV solution (also blood) accumulated in the extracellular fluid (ECF) (including interstitial fluid). Secondly, the resistance ratio started to increase at infiltration, showing the highest value after 1.4 min of infiltration, and gradually decreased thereafter. Thirdly, the impedance ($Z_C$) of cell membrane decreased significantly (especially at 50 kHz) during infiltration and gradually decreased thereafter. Fourthly, Cole-Cole plot indicated that the positions of (R, $X_C$) shifted toward left owing to infiltration, reflecting the IV solution accumulated in the ECF. The resistance ($R_0$) at zero frequency decreased continuously over time, indicating that it is a vital impedance parameter capable of detecting early infiltration during IV infusion. Finally, the mechanism of the current flowing through the ECF, cell membrane, and ICF in the subcutaneous tissues was analyzed as a function of time before and after infiltration, using an equivalent circuit model of the human cell. In conclusion, it was confirmed that the infiltration could be detected early using these impedance parameters during the infusion of IV solution.

Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis Using the Quantamatrix Multiplexed Assay Platform System

  • Wang, Hye-young;Uh, Young;Kim, Seoyong;Cho, Eunjin;Lee, Jong Seok;Lee, Hyeyoung
    • Annals of Laboratory Medicine
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    • 제38권6호
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    • pp.569-577
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    • 2018
  • Background: The increasing prevalence of drug-resistant tuberculosis (TB) infection represents a global public health emergency. We evaluated the usefulness of a newly developed multiplexed, bead-based bioassay (Quantamatrix Multiplexed Assay Platform [QMAP], QuantaMatrix, Seoul, Korea) to rapidly identify the Mycobacterium tuberculosis complex (MTBC) and detect rifampicin (RIF) and isoniazid (INH) resistance-associated mutations. Methods: A total of 200 clinical isolates from respiratory samples were used. Phenotypic anti-TB drug susceptibility testing (DST) results were compared with those of the QMAP system, reverse blot hybridization (REBA) MTB-MDR assay, and gene sequencing analysis. Results: Compared with the phenotypic DST results, the sensitivity and specificity of the QMAP system were 96.4% (106/110; 95% confidence interval [CI] 0.9072-0.9888) and 80.0% (72/90; 95% CI 0.7052-0.8705), respectively, for RIF resistance and 75.0% (108/144; 95% CI 0.6731-0.8139) and 96.4% (54/56; 95% CI 0.8718-0.9972), respectively, for INH resistance. The agreement rates between the QMAP system and REBA MTB-MDR assay for RIF and INH resistance detection were 97.6% (121/124; 95% CI 0.9282-0.9949) and 99.1% (109/110; 95% CI 0.9453-1.0000), respectively. Comparison between the QMAP system and gene sequencing analysis showed an overall agreement of 100% for RIF resistance (110/110; 95% CI 0.9711-1.0000) and INH resistance (124/124; 95% CI 0.9743-1.0000). Conclusions: The QMAP system may serve as a useful screening method for identifying and accurately discriminating MTBC from non-tuberculous mycobacteria, as well as determining RIF- and INH-resistant MTB strains.