• Honam Mathematical Journal
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• v.20 no.1
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• pp.45-55
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• 1998

• Journal of Microbiology and Biotechnology
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• v.8 no.3
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• pp.214-221
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• 1998

A Brevibacterium ammoniagenes gene coding for glucose/mannose-specific enzyme II ($EII^{Glc}$) of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned by complementing an Escherichia coli mutation affecting a ptsG gene, and the complete DNA nucleotide sequence was determined. The cloned gene was identified to be a ptsG, which enables the E. coli transportment to use glucose more efficiently than mannose as the sole carbon source in an M9 minimal medium. The ptsG gene of B. ammoniagenes consists of an open reading frame of 1,983 nucleotides putatively encoding a polypeptide of 661 amino acid residues and a TAA stop codon. The deduced amino acid sequence of the B. ammoniagenes $EII^{Glc}$ shows, at $46\%$, the highest degree of sequence similarity with the Corynebacterium glutamicum EII specific for both glucose and mannose. In addition, the $EII^{Glc}$ shares approximately $30\%$ sequence similarities with sucrose-specific and ${\beta}$-glucoside-specific EIIs of the several bacteria belonging to the glucose-PTS class. The 161-amino-acid C-terminal sequence of $EII^{Glc}$ is also similar to that of E. coli enzyme $IIA^{Glc}$, specific for glucose ($EIIA^{Glc}$). The B. ammoniagenes $EII^{Glc}$ consists of three domains; a hydrophobic region (EIIC) and two hydrophilic regions (EIIA, EIIB). The arrangement of structural domains, IIBCA, of the $EII^{Glc}$ is identical to those of EIIs specific for sucrose or ${\beta}$-glucoside. While the domain IIA was removed from the B. ammoniagenes $EII^{Glc}$ the remaining domains IIBC were found to restore the glucose and mannose-utilizing capacity of E. coli mutant lacking $EII^{Glc}$ activity with $EIIA^{Glc}$ of the E. coli mutant. $EII^{Glc}$ contains a histidine residue and a cysteine residue which are putative phosphorylation sites for the protein.

• Journal of Korean Society of Forest Science
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• v.104 no.2
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• pp.213-220
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• 2015

This study was carried out to determine the distribution of factors as effected by debris flow in Ulsan and Pusan metropolitan areas because mainly debris flow caused by typhoons and local heavy rainfall events is mainly attributed to damage of human being ad property. The high risk degree of debris flow was to affected by east (20%), northeast (20%) and northwest (20%) slopes with stand age class with elevation (69%) of 100-200 (33%). Also, the risk was high in high erosion collapse degree with slope degree of $20-25^{\circ}$ with over 300 mm (100%) of maximum daily rainfall events and 50-100 mm (50%) or >100 mm (50%) of maximum hourly rainfall events with <5 km of stream path and <50 ha of catchment area. Landslide debris and wood residue flow was also related to igneous rocks (73%) and bank collapse types of debrs flow (57%).