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Enzymatic Hydrolysis of Pretreated Chitin by Aspergillus carneus Chitinase

  • Mohamed, Abdel-Naby;Kwon, Dae-Young
    • Journal of Microbiology and Biotechnology
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    • v.2 no.3
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    • pp.197-203
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    • 1992
  • Studies of the pretreatment of chitin and its subsequent hydrolysis by Aspergillus carneus chitinase are reported. Ball milling was found to be the most effective way among the pretreatment methods tested. Data are presented describing the effect of enzyme and substrate concentrations on the rate and extent of the hydrolysis process. It was found that the successive addition of enzyme improved the saccharification yield. Significant product inhibition of the chitinase was observed when N-acetylglucosamine concentration was 3.6% or higher. Adsorption of enzymes to the substrate occurred during a 24 hr hydrolysis period. An initial rapid and extensive adsorption occurred, followed by gradual desorption which increased during the time of reaction. Intermediate removal of the hydrolyzate and continuation of the hydrolysis by adsorbed enzyme on the residual chitin was also investigated. A total of 75.4 g/l reducing sugars, corresponding to 69.2% saccharificaton yield (as N-acetylglucosamine) was obtained. In addition an increase in the amount of recoverable enzymes was observed under these conditions. Evidence presented here suggests that the technique, whereby the free enzymes in the recovered hydrolyzate are re-adsorbed onto the new substrate, may provide a means of recirculating the dissolved enzymes.

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Outlier Detection Using Support Vector Machines (서포트벡터 기계를 이용한 이상치 진단)

  • Seo, Han-Son;Yoon, Min
    • Communications for Statistical Applications and Methods
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    • v.18 no.2
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    • pp.171-177
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    • 2011
  • In order to construct approximation functions for real data, it is necessary to remove the outliers from the measured raw data before constructing the model. Conventionally, visualization and maximum residual error have been used for outlier detection, but they often fail to detect outliers for nonlinear functions with multidimensional input. Although the standard support vector regression based outlier detection methods for nonlinear function with multidimensional input have achieved good performance, they have practical issues in computational cost and parameter adjustments. In this paper we propose a practical approach to outlier detection using support vector regression that reduces computational time and defines outlier threshold suitably. We apply this approach to real data examples for validity.

A Statistical Approach to Examine the Impact of Various Meteorological Parameters on Pan Evaporation

  • Pandey, Swati;Kumar, Manoj;Chakraborty, Soubhik;Mahanti, N.C.
    • The Korean Journal of Applied Statistics
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    • v.22 no.3
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    • pp.515-530
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    • 2009
  • Evaporation from surface water bodies is influenced by a number of meteorological parameters. The rate of evaporation is primarily controlled by incoming solar radiation, air and water temperature and wind speed and relative humidity. In the present study, influence of weekly meteorological variables such as air temperature, relative humidity, bright sunshine hours, wind speed, wind velocity, rainfall on rate of evaporation has been examined using 35 years(1971-2005) of meteorological data. Statistical analysis was carried out employing linear regression models. The developed regression models were tested for goodness of fit, multicollinearity along with normality test and constant variance test. These regression models were subsequently validated using the observed and predicted parameter estimates with the meteorological data of the year 2005. Further these models were checked with time order sequence of residual plots to identify the trend of the scatter plot and then new standardized regression models were developed using standardized equations. The highest significant positive correlation was observed between pan evaporation and maximum air temperature. Mean air temperature and wind velocity have highly significant influence on pan evaporation whereas minimum air temperature, relative humidity and wind direction have no such significant influence.

Evaluation of the Stress Occurring Phenomenon for Cold-Rolled Carbon Steel During a Laser Welding Process (냉연 압연 강판의 레이저 용접 공정 시 발생하는 응력 평가)

  • Lee, Chulku;Lee, Wooram
    • Journal of Welding and Joining
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    • v.31 no.1
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    • pp.51-57
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    • 2013
  • Residual stress caused in the weldments with high restraint force are often observed during welding in the weldments of Inner and outdoor materials or radial tanks. The reason is that quantitative analysis about thermal stresses during laser welding is lacking for this weldments. To verify Finite Elements Method (FEM) theory, the temperature was measured with thermocouple in a real time in this paper. Also analysis of the thermal stress for welding condition is performed by Comsol program package on various welding condition in SCP1-S butt welding. The principal stress in laser welding process is seen through the width direction. Also, it was confirmed that a change in base metal by thermal expansion made the stress in width direction stronger. Base metal close to the weld bead as the process progresses to the tensile stress in the compressive stress was varied. It was shown that the change of stress was quantitative from the bead at a certain distance.

Recurrent Ant Colony Optimization for Optimal Path Convergence in Mobile Ad Hoc Networks

  • Karmel, A;Jayakumar, C
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.9
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    • pp.3496-3514
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    • 2015
  • One of the challenging tasks in Mobile Ad hoc Network is to discover precise optimal routing solution due to the infrastructure-less dynamic behavior of wireless mobile nodes. Ant Colony Optimization, a swarm Intelligence technique, inspired by the foraging behaviour of ants in colonies was used in the past research works to compute the optimal path. In this paper, we propose a Recurrent Ant Colony Optimization (RECACO) that executes the actual Ant Colony Optimization iteratively based on recurrent value in order to obtain an optimal path convergence. Each iteration involves three steps: Pheromone tracking, Pheromone renewal and Node selection based on the residual energy in the mobile nodes. The novelty of our approach is the inclusion of new pheromone updating strategy in both online step-by-step pheromone renewal mode and online delayed pheromone renewal mode with the use of newly proposed metric named ELD (Energy Load Delay) based on energy, Load balancing and end-to-end delay metrics to measure the performance. RECACO is implemented using network simulator NS2.34. The implementation results show that the proposed algorithm outperforms the existing algorithms like AODV, ACO, LBE-ARAMA in terms of Energy, Delay, Packet Delivery Ratio and Network life time.

Characteristics of Poly(methylphenyl)silane Photoreists (Poly(methylpheny) Silane Photoresist의 특성)

  • Kang, Doo-Whan;Chung, Nak-Jin
    • Applied Chemistry for Engineering
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    • v.1 no.2
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    • pp.161-167
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    • 1990
  • Polymethylphenylsilane(PMPS) was synthesized with methylphenyldichlorosilane using metal sodium. Various sample coated on quartz plate were exposed and the yield of residual film was calculated. To obtain the fine image forming after developing and drying, optical transmittance characterization have to be considered. Exposure is described by three optical parameters X, Y, and Z. There parameters are normally determined from optical transmittance measurements of exposed PMPS films. Z parameter was determined from the initial slope of a transmittance according to exposure time. This set of function parameters provided a complete description of photoresist exposure and development and became the basis for the theoretical process models.

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Application of Zeolite with Different Cation Exchange Capacity for the Stabilization of Heavy Metals in Upland Soil (양이온교환용량이 다른 제올라이트 처리에 따른 밭토양 내 중금속 안정화 평가)

  • Gu, Bon-Wun;Kim, Mun-Ju;Park, Seong-Jik
    • Journal of The Korean Society of Agricultural Engineers
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    • v.59 no.5
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    • pp.41-49
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    • 2017
  • This study was aimed to investigate the influence of cation exchange capacity (CEC) and application amounts of zeolite on the stabilization of heavy metals (As, Ni, Pb, and Zn) in upland soils. The upland soils were sampled from field near mines located in Gyeonggi Province. The CEC of zeolite was treated at three different levels, ie, low, medium, and high, while zeolite was amended with soils at the ratio of 0.1 % and 0.5 % as to soil weight. A sequential extraction was performed for the soil sampled at 1, 2 4, and 8 week after zeolite was added to the soil. The concentrations of Pb and Zn appeared to be high in the sampled soils. The mobility of heavy metals obtained from sequential experiments was as follows: Pb > Zn > Ni >As. Addition of zeolite to contaminated soils effectively reduced exchangeable and carbonate fractions but increased organic and residual fraction, indicating that zeolite is effective for immobilizing heavy metals in soils. The influence of incubation time on the metal stabilization was rather pronounced as compared to the application amount and CEC of zeolite.

The effect of step heat treatment in the critical current density of BSCCO 2223 tapes (BSCCO 2223선재의 임계전류밀도에 영향을 미치는 단계별 열처리의 효과)

  • 박성창;유재무;고재웅;김영국;김철진
    • Progress in Superconductivity
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    • v.4 no.1
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    • pp.90-93
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    • 2002
  • The sintering process of BSCCO 2223 tapes is a complex process that is very sensitive to parameters, such as temperature, oxygen partial pressure, heating and cooling rate and holding time. During the first heat treatment, 2212 phase of precursor powder is partially transformed into 2223 phase and some residual secondary phases, such as $(Bi,Pb)_2$$Sr_2$CuO/sub y/(2201), $(Ca,Sr)_2$CuO/sub y/(2/1AEC), (Ca,Sr)/sub 14/Cu/sub 24/O/sub 41/(14/24 AEC) etc. The secondary phases are difficult to be removed from the BSCCO 2223 matrix on the heat treatment. These secondary phases degrade the critical current density. In order to minimize the amount and size of alkaline earth cuprate(AEC) particles step heat treatment is applied during the first heat treatment under the varying atmosphere. Experimental results showed that by adapting the step heat treatment process, the amount and particle size of the secondary phases in the final tapes are decreased. Consequently, the BSCCO 2223grain texture and Jc properties are improved.

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Development of Rotor Shaft Manufacturing Process using a Large Friction Welding (대형마찰용접을 이용한 로타샤프트 제조공정개발)

  • Jeong, H.S.;Lee, N.K.;Park, H.C.;Choi, S.K.;Cho, J.R.
    • Transactions of Materials Processing
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    • v.16 no.4 s.94
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    • pp.266-270
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    • 2007
  • Inertia welding is a solid-state welding process in which butt welds in materials are made in bar and in ring form at the joint face, and energy required for welding is obtained from a rotating flywheel. The stored energy is converted to frictional heat at the interface under axial load. The quality of the welded joint depends on many parameters, including axial force, initial revolution speed and energy, amount of upset, working time, and residual stresses in the joint. Inertia welding was conducted to make the large rotor shaft for low speed marine diesel engine, alloy steel for shaft of 140mm. Due to material characteristics, such as, thermal conductivity and high temperature flow stress, on the two sides of the weld interface, modeling is crucial in determining the optimal weld parameters. FE simulation is performed by the commercial code DEFORM-2D. A good agreement between the predicted and actual welded shape is observed. It is expected that modeling will significantly reduce the number of experimental trials needed to determine the weld parameters.

A Study on efficacy and safety of antibacterial(pefloxacin methanesulfonate) to cultured fish, Cyprinus caprio and Paralichthys olivaceus (양식어류(이스라엘 잉어, 넙치)에 대한 항균물질 pefloxacin의 효능 및 안전성에 관한 연구)

  • Heo, Gang-joon;Kim, Jeong-ho
    • Korean Journal of Veterinary Research
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    • v.34 no.1
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    • pp.153-163
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    • 1994
  • A study on quinolone antibacterial (pefloxacin methanesulfonate) was performed to use for the drug of fisheries. Petloxacin was proved excellent in antibacterial activity and resistance against fish pathogens when compared with the existing antibacterials. And any side effect was not observed during the period of indicated use. An outline of MIC(Minimal Inhibitory Concentration) was $1.6{\sim}6.4{\mu}g/ml$, $TLm_{48h}$ value were 380~420 ppm in Israeli carp(Cyprinus caprio) and 2100~2300 ppm in flounder(Paralichthys olivaceus). The residual time of fish body was less than 15 days. So we can treat some bacterial disease of fish by the dosage of 100 g/day/ton of fish body weight for 3 days and pefloxacin is thought to be used effectively and widely against most bacterial fish pathogens.

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