• Title/Summary/Keyword: research utilization

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A Substrate Fed-Batch Biphasic Catalysis Process for the Production of Natural Crosslinking Agent Genipin with Fusarium solani ACCC 36223

  • Zhu, Yuyao;Zhao, Botao;Huang, Xiaode;Chen, Bin;Qian, Hua
    • Journal of Microbiology and Biotechnology
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    • v.25 no.6
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    • pp.814-819
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    • 2015
  • The natural crosslinking agent genipin has been applied widely in biomedicines and foods nowadays. Because of the special hemiacetal ring structure in its molecule, it can only be prepared by hydrolysis of geniposide according to biocatalysis. In this research, strategies including aqueous-organic biphasic catalysis and substrate fed-batch mode were adopted to improve the biocatalysis process of genipin. A 10 L ethyl acetate-aqueous biphasic system with geniposide fed-batch led to a satisfying genipin yield. With Fusarium solani ACCC 36223, 15.7 g/l genipin in the ethyl acetate phase was obtained, corresponding to space-time yields of 0.654 g l-1 h-1.

An in vitro Actinidia Bioassay to Evaluate the Resistance to Pseudomonas syringae pv. actinidiae

  • Wang, Faming;Li, Jiewei;Ye, Kaiyu;Liu, Pingping;Gong, Hongjuan;Jiang, Qiaosheng;Qi, Beibei;Mo, Quanhui
    • The Plant Pathology Journal
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    • v.35 no.4
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    • pp.372-380
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    • 2019
  • Pseudomonas syringae pv. actinidiae (Psa) is by far the most important pathogen of kiwifruit. Sustainable expansion of the kiwifruit industry requires the use of Psa-tolerant or resistant genotypes for the breeding of tolerant cultivars. However, the resistance of most existing kiwifruit cultivars and wild genotypes is poorly understood, and suitable evaluation methods of Psa resistance in Actinidia have not been established. A unique in vitro method to evaluate Psa resistance has been developed with 18 selected Actinidia genotypes. The assay involved debarking and measuring the lesions of cane pieces inoculated with the bacterium in combination with the observation of symptoms such as callus formation, sprouting of buds, and the extent to which Psa invaded xylem. Relative Psa resistance or tolerance was divided into four categories. The division results were consistent with field observations. This is the first report of an in vitro assay capable of large-scale screening of Psa-resistance in Actinidia germplasm with high accuracy and reproducibility. The assay would considerably facilitate the breeding of Psa-resistant cultivars and provide a valuable reference and inspiration for the resistance evaluation of other plants to different pathogens.

Design, Synthesis and Evaluation of Pentacyclic Triterpenoids Similar to Glycyrrhetinic Acid Via Combination of Chemical and Microbial Modification as Glycogen Phosphorylases Inhibitor

  • Zhu, Yuyao;Zhang, Jian;Huang, Xiaode;Chen, Bin;Qian, Hua;Zhao, Botao
    • Journal of Microbiology and Biotechnology
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    • v.28 no.11
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    • pp.1876-1882
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    • 2018
  • A series of pentacyclic triterpenoids similar to glycyrrhetinic acid were designed and synthesized via the combination of chemical modification and microbial catalysis. All products were screened for the glycogen phosphorylases inhibitory activities in vitro. Within this series of derivatives, compound 5 displayed good inhibitory activities with $IC_{50}$ value of $27.7{\mu}M$, which is better than that of the other derivatives and glycyrrhetinic acid. Structure-activity relationship (SAR) analysis of these inhibitors was also discussed.

Study on Internal Reforming Characteristic of 1 kW Solid Oxide Fuel Cell Stack (1 kW 고체산화물 연료전지 스택의 내부개질 특성 연구)

  • CHOI, YOUNGJAE;AHN, JINSOO;LEE, INSUNG;BAE, HONGYOUL;MOON, JIWOONG;LEE, JONGGYU
    • Transactions of the Korean hydrogen and new energy society
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    • v.28 no.4
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    • pp.377-383
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    • 2017
  • This paper presents the performance characteristics of a 1 kW solid oxide fuel cell (SOFC) stack under various internal reforming and fuel utilization conditions. The Research Institute of Industrial Science & Technology (RIST) developed the 9-cell stack using a $20{\times}20cm^2$ anode supported planar cell with an active area of $324cm^2$. In this work, current-voltage characteristic test, fuel utilization test, continuous operation, and internal reforming test were carried out sequentially for 765 hours at a furnace temperature of $700^{\circ}C$. The influence of fuel utilization and internal reforming on the stack performance was analyzed. When the 1 kW stack was tested at a current of 145.8 A with a corresponding fuel utilization of 50-70% (internal reforming of 50%) and air utilization of 27%, the stack power was approximately 1.062-1.079 kW. Under continuous operation conditions, performance degradation rate was 2.16%/kh for 664 hours. The internal reforming characteristics of the stack were measured at a current of 145.8. A with a corresponding fuel utilization of 60-75%(internal reforming of 50-80%) and air utilization of 27%. As fuel utilization and internal reforming ratio increased, the stack power was decreased. The stack power change due to the internal reforming ratio difference was decreased with increasing fuel utilization.

How the FTA's Utilization in Contract for the International Sale of Goods of Korea's Companies Affects Their Export Performance

  • Park, Jin-Woo;Pak, Myong-Sop
    • Journal of Korea Trade
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    • v.23 no.4
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    • pp.80-102
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    • 2019
  • Purpose - This paper aims to articulate relationship about factors influencing FTA utilization by dividing them into company's external and internal factors and performing investigation on the relationship between FTA utilization and export performance. Design/methodology - This study verified factors influencing FTA utilization by dividing them into company's external and internal factors and performing investigation on the relationship between FTA utilization and export performance. Empirical analysis was performed by setting internal and external factors required for FTA utilization as variables. To achieve this, research model was established based on previous study, hypothesis was deduced, and statistical program were used to test the hypothesis. This study performed empirical analysis using statistical program of SPSS 18.0 and AMOS 18.0 for the research model. Findings - Empirical analysis was performed regarding the effect of the FTA utilization on export performance and previous study defined export performance as the company's increased economy benefits through export and increase in new transactions. Analysis was also performed for factors affecting the FTA utilization by the company and through management and response of external factors and internal factors it was confirmed that the FTA utilization by the company led to increase in the company's export performance as a result. This study proposes a method to achieve export performance based on this. Originality/value - Companies seeking to utilize the FTA sign the Contract for the International Sale of goods and there are many conditions to meet in order to receive trade preferences during the transaction process. Existing trade order and order in the FTA have to be followed. Country of origin can be seen as key in the FTA. The Rule of Origin becomes the most important evaluation standard in applying preferential tariff in the FTA. Such regulations can be seen as external factors which cannot be controlled by the company. Internal factors are capabilities owned before that can be controlled by the company. The study sought to test the variables regarding factors centered on such capability. This study verified factors influencing FTA utilization by dividing them into company's external and internal factors and performing investigation on the relationship between FTA utilization and export performance.

Synthesis of Multiwall Carbon Nanotube/Graphene Composite by Aerosol Process and Its Characterization for Supercapacitors (에어로졸 공정에 의한 Multiwall carbon nanotube/Graphene 복합체 제조 및 슈퍼커패시터 특성평가)

  • Jo, Eun Hee;Kim, Sun Kyung;Chang, Hankwon;Lee, Chong Min;Park, Su-Ryeon;Choi, Ji-hyuk;Jang, Hee Dong
    • Particle and aerosol research
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    • v.12 no.4
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    • pp.127-134
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    • 2016
  • A multiwall carbon nanotube (MWCNT)/graphene (GR) composite was synthesized for an enhanced supercapacitor. Aerosol spray pyrolysis (ASP) was employed to synthesize the MWCNT/GR composites using a colloidal mixture of MWCNT and graphene oxide (GO). The effect of the weight ratio of the MWCNT/GO on the particle properties including the morphology and layered structure were investigated. The morphology of MWCNT/GR composites was generally the shape of a crumpled paper ball, and the average composite size was about $5{\mu}m$. MWCNT were uniformly dispersed in GR sheets and the MWCNT not only increase the basal spacing but also bridge the defects for electron transfer between GR sheets. Thus, it was increasing electrolyte/electrode contact area and facilitating transportation of electrolyte ion and electron in the electrode. Electrochemical data demonstrate that the MWCNT/GR (weight ratio=0.1) composite possesses a specific capacitance of 192 F/g at 0.1 A/g and good rate capability (88% capacity retention at 4 A/g) using two-electrode testing system.