• Journal of Veterinary Science
• /
• v.21 no.2
• /
• pp.26.1-26.14
• /
• 2020

Pancreatic ductal adenocarcinoma is a lethal cancer type that is associated with multiple gene mutations in somatic cells. Genetically engineered mouse is hardly applicable for developing a pancreatic cancer model, and the xenograft model poses a limitation in the reflection of early stage pancreatic cancer. Thus, in vivo somatic cell gene engineering with clustered regularly interspaced short palindromic repeats is drawing increasing attention for generating an animal model of pancreatic cancer. In this study, we selected Kras, Trp53, Ink4a, Smad4, and Brca2 as target genes, and applied Campylobacter jejuni Cas9 (CjCas9) and Streptococcus pyogens Cas9 (SpCas9) for developing pancreatic cancer using adeno associated virus (AAV) transduction. After confirming multifocal and diffuse transduction of AAV2, we generated SpCas9 overexpression mice, which exhibited high double-strand DNA breakage (DSB) in target genes and pancreatic intraepithelial neoplasia (PanIN) lesions with two AAV transductions; however, wild-type (WT) mice with three AAV transductions did not develop PanIN. Furthermore, small-sized Cjcas9 was applied to WT mice with two AAV system, which, in addition, developed high extensive DSB and PanIN lesions. Histological changes and expression of cancer markers such as Ki67, cytokeratin, Mucin5a, alpha smooth muscle actin in duct and islet cells were observed. In addition, the study revealed several findings such as 1) multiple DSB potential of AAV-CjCas9, 2) peri-ductal lymphocyte infiltration, 3) multi-focal cancer marker expression, and 4) requirement of > 12 months for initiation of PanIN in AAV mediated targeting. In this study, we present a useful tool for in vivo cancer modeling that would be applicable for other disease models as well.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.35 no.4
• /
• pp.75-83
• /
• 2023

Apart from implementing hardware solutions like raising the crest freeboard of coastal structures to efficiently counter wave-overtopping, there is a simultaneous requirement for software-driven disaster mitigation strategies. These tactics involve the swift and accurate dissemination of wave-overtopping information to the inland regions of coastal zones, enabling the regulation of evacuation procedures and movement. In this study, a method was proposed to estimate wave-overtopping by utilizing the temporal variation of wave heights exceeding the structure's crown level, with the aim of developing an on-site wave measurement system for providing wave-overtopping information in the field. Laboratory model experiments were conducted on vertical seawall structures to measure wave-overtopping volumes and wave runup heights under different wave conditions and structural freeboard variations. By assuming that the velocity of water inundation on the top of the structure during wave-overtopping events is equivalent to the long-wave velocity, an overtopping discharge coefficient was introduced. This coefficient was utilized to estimate the rate of wave-overtopping based on the temporal changes in wave runup heights measured at the top of the structure. Upon reasonably calculating the overtopping discharge coefficient, it was verified that the estimation of wave-overtopping could be achieved solely based on the wave runup heights.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.14 no.3
• /
• pp.767-773
• /
• 2010

RFID and Kiosk has been acquiring the essential position not only for providing simple information but also as the tool for establishing Ubiquitous based society. Especially it can be said that the time for developing various Kiosks as important methods to conducting marketing and service through the differentiation and easiness on dealing information has come. Considering the present requirement, 'U-Commerce System' has been designed and developed. In the system, the RFID reader installed in 'U-Shop Kiosk System' recognizes the RFID tag on situated the specific products and detail product information and related multimedia advertisement are provided. Moreover, when the customers have the needs to purchase concerned products on the spot, they can pay and order by using credit cards. By employing the system, the amount of stock and delivery condition can be monitored in real time and the gathered information in DB can be used for dealing with stock related matters. In addition, a system can be developed to monitor and manage the remote shops in real time base. Furthermore, to overcome the limit of simple unmanned ubiquitous style Kiosk, the possibility of developing an expansion model of ubiquitous style CRM Kiosk has been suggested.

• Journal of KIISE:Computing Practices and Letters
• /
• v.14 no.1
• /
• pp.106-110
• /
• 2008

The requirement for customized learning environments is increasing with the development of Web 2.0 technology To personalize services in E-learning, there are various functions available to the user. However, some components have fixed or inflexible functionality that limits what can be changed, and customization information has not been used for other purposes. In this paper, we propose an e-Space manager for the dynamic customization of learning environment control units. The proposed method can confirm which areas users have customized and how they have customized them. The advantage is that users can control their own learning environments, including not only the format, but also the content within the limits of the underlying system. The customization information is captured to confirm the competency model.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.10 no.1
• /
• pp.39-50
• /
• 2010

In this paper, we analyze and simulate the performance of a tactics mobile communication system based on ultra wide band (UWB) in multi-user interference (MUI) environments. This system adopts a double binary turbo code for forward error correction (FEC). Wireless channel is modeled a modified Saleh and Valenzuela (SV) model. We employ a space time block coding (STBC) scheme for enhancing system performance. System performance is evaluated in terms of bit error probability. From the simulation results, it is confirmed that the tactics mobile communication system based on UWB, which is encoded with the double binary turbo code, can achieve a remarkable coding gain with reasonable encoding and decoding complexity in multi-user interference environments. It is also known that the bit error probability performance of the tactics mobile communication system based on UWB can be substantially improved by increasing the number of iterations in the decoding process for a fixed cod rate. Besides, we can demonstrate that the double binary turbo coding scheme is very effective for increasing the number of simultaneous users for a given bit error probability requirement.

• Management & Information Systems Review
• /
• v.29 no.4
• /
• pp.207-223
• /
• 2010

One of the important prudential regulations is the capital regulation. The current domestic and international capital regulation sets the minimum capital requirement according to the size of risk which is the simple sum of market risk and credit risk. However the portfolio theory suggests that, due to the effect of diversification, the total risk is less than the summation of market and credit risk. This paper investigates and does empirical test to verify the diversification effect in measuring financial firm's integrated risk. We verify the diversification effect between the market risk and credit risk. This paper's contribution is to present the empirical evidence that, considering the relationship between market and credit risk, the integrated risk is less than sum of them. This implication is that the surplus capital may be used for the other purposes, therefore enhancing capital allocation efficiency in view of society as a whole.

• International Journal of Fluid Machinery and Systems
• /
• v.6 no.2
• /
• pp.87-93
• /
• 2013

In order to study the effect of the fluid-structure interaction (FSI) on the simulation results, the external characteristics and internal flow features of a diffuser pump were analyzed with a two-way flow solid coupling method. And the static and dynamic structure analysis of the blade was also caculated with the FEA method. The steady flow field is based on Reynolds Averaged N-S equations with standard $k-{\varepsilon}$ turbulent model, the unsteady flow field is based on the large eddy simulation, and the structure response is based on elastic transient structural dynamic equation. The results showed that the effect of FSI on the head prediction based on CFD really exists. At the same radius, the van mises stress on the nodes closed shroud and hub was larger than other nodes. A large deformation region existed near inlet side at the middle of blades. The strength of impeller satisfied the strength requirement with static stress analysis based on the fourth strength theory. The dynamic stress varied periodically with the impeller rotating. It was also found that the fundamental frequency of the dynamic stress is the rotating frequency and its harmonic frequency. The frequency of maximum stress amplitude at node 1626 was 7 times of the rotating frequency. The frequency of maximum stress amplitude at node 2328 was 14 times of the rotating frequency. No matter strength failure or fatigue failure, the root of blades near shroud is the key region to analyse.

• Journal of Digital Convergence
• /
• v.10 no.5
• /
• pp.207-213
• /
• 2012

ICT industry in Korea was constituted an industry in early 1980's and has been growing more and more since then. In the process of this growth, most of the large ICT companies in Chaebeols has been absorbing in undemocratic economic behaviors. As a result, the sales and the profit of Cheabeols and large companies has been increasing by geometrical progression, but most of small and medium cooperative companies of these big corporations have been eking a scanty existence. And many of them had gone belly up. This article will propose the digital economic democratization as an alternative to overcome the present undemocratic economic situation in the digital economic field and to develop the healthy ICT industry, and to preliminarily study the concept, elements and meaning of the digital economic democratization.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.18 no.1
• /
• pp.37-43
• /
• 2014

In current seismic design code, steel moment frames are classified into ordinary, intermediate, and special moment frames. In the case of special moment frames which have large R-factor, economic design is possible by reducing the design lateral force. However, there is difficulty for practical application due to constraints such as strong column-weak beam requirement. This study evaluated if steel intermediate moment frame could maintain enough seismic capacity when the R-factor is increased from 4.5 to 6. As for the analytical models, steel moment frames of 3 and 5 stories were categorized into four performance groups according to seismic design category. Seismic performances of the frames were evaluated through the procedure based on FEMA P695. FEMA P695 utilizes nonlinear static analysis(pushover analysis) and nonlinear dynamic analysis(incremental dynamic analysis, IDA). In order to reflect the characteristics of Korean steel moment frames on the analytical model, the beam-column connection was modeled as weak panel zone where the collapse of panel zone was indirectly considered by checking its ultimate rotational angle after an analysis is done. The analysis result showed that the performance criteria required by FEMA P695 was satisfied when R-factor increased in all the soil conditions except $S_E$.

• Journal of the Korea Computer Graphics Society
• /
• v.2 no.1
• /
• pp.24-30
• /
• 1996

The ray tracing method, which is one of many photorealistic rendering techniques, requires heavy computational processing to synthesize images. Parallel processing can be used to reduce the computational processing time. A parallel algorithm for the ray tracing has been implemented and executed for various images on transputer systems. In order to develop a scalable parallel algorithm, a processor farming technique has been exploited. Since each image is divided and distributed to each farming processor, the scalability of the parallel system and load balancing are achieved naturally in the proposed algorithm. Efficiency of the parallel algorithm is obtained up to 95% for nine processors. However, the best size of a distributed task is much higher in simple images due to less computational requirement for every pixel. Efficiency degradation is observed for large granularity tasks because of load unbalancing caused by the large task. Overall, transputer systems behave as good scalable parallel processing system with respect to the cost-performance ratio.