• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.892-897
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• 2005

Macrophage colony-stimulating factor (M-CSF) is a growth factor required for growth and differentiation of mononuclear phagocyte lineage. Total and 16 poly (A) mRNA of bovine M-CSF were isolated from healthy bovine peripheral mononuclear cells stimulated by phobol 12-myristste 13-acetate (TPA). The more compatible cultured mononuclear cells were 5${\times}$10/ml for RNA isolation. TPA-activated mononuclear cells increased the level of M-CSF-mRNA more than concanavalin A (Con A) and lipopolysaccharide (LPS). The optimal analysis of reverse transcriptase-polymerase chain reaction (RT-PCR) for14 Macrophage colonystimulating factor (M-CSF) as a growth factor required for bovine M-CSF was denaturation at 94$^{\circ}C$ for 1 minute, annealing at 57$^{\circ}C$ for 1 minute, extension at 72$^{\circ}C$ for 1 minute for 30 cycles. The size of cDNA of bovine M-CSF by RT-PCR was 774 base pairs. A 774 base pairs cDNA encoding bovine M-CSF was synthesized by reverse transcriptase polymerase chain reaction (RT-PCR). Ligated cDNA was transformed to competent cells and then plasmid isolation and digestion was performed. Molecular cloning and sequencing were performed for cDNA of bovine M-CSF. The size of cloned cDNA of bovine M-CSF was 774base pairs. The homology of base sequence and amino acid sequence was 88% and 86% compared with known human M-CSF, respectively. From a high degree of sequence similarity, the obtained cDNA of bovine M-CSF is thought be a specific gene of bovine M-CSF.

• The Plant Pathology Journal
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• v.30 no.3
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• pp.236-244
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• 2014

The plant pathogen Fusarium graminearum causes Fusarium head blight in cereal crops and produces mycotoxins that are harmful to animals and humans. For the initiation and spread of disease, asexual and sexual reproduction is required. Therefore, studies on fungal reproduction contribute to the development of new methods to control and maintain the fungal population. Screening a previously generated transcription factor mutant collection, we identified one putative $C_2H_2$ zincfinger transcription factor, pcs1, which is required for both sexual and asexual reproduction. Deleting pcs1 in F. graminearum resulted in a dramatic reduction in conidial production and a complete loss of sexual reproduction. The pathways and gene ontology of pcs1-dependent genes from microarray experiments showed that several G-protein related pathways, oxidase activity, ribosome biogenesis, and RNA binding and processing were highly enriched, suggesting that pcs1 is involved in several different biological processes. Further, overexpression of pcs1 increased conidial production and resulted in earlier maturation of ascospores compared to the wild-type strain. Additionally, the vegetative growth of the overexpression mutants was decreased in nutrient-rich conditions but was not different from the wild-type strain in nutrient-poor conditions. Overall, we discovered that the pcs1 transcription factor positively regulates both conidiation and sexual reproduction and confers nutrient condition-dependent vegetative growth.

• Journal of the Korean Society of Propulsion Engineers
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• v.17 no.5
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• pp.80-85
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• 2013

This paper describes a probabilistic structural design method of composite propulsion system by comparing safety factor based on average value and allowable value with structural reliability. Generally, the required structural safety factor and reliability of composite pressure vessel are 1.5 and 0.999, respectively. In the case of structural design using average strength, the safety factor which satisfies the required structural reliability depends on the variation of fiber strength. However, the structural design using allowable value shows constant safety factor for the variation of fiber strength, because the allowable value of fiber strength is calculated by considering the variation of fiber strength. Through the analysis results, it was known that the fiber strength is the most important design random variable for the structural design of composite pressure vessel and the variation of fiber strength must be minimized to develop the high performance composite propulsion system.

• Journal of the Korean Statistical Society
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• v.29 no.1
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• pp.95-108
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• 2000

Consider the oblique factor model X=Af＋$\varepsilon$, with defining relation $\Sigma$=Λ$\Phi$Λ'＋Ψ. This paper is concerned with suggesting an optimal Bayes criterion for determining the number of factors in the model, i.e. dimension of the vector f. The use of marginal likelihood as a method for calculating posterior probability of each model with given dimension is developed under a generalized conjugate prior. Then based on an appropriate loss function, a Bayes rule is developed by use of the posterior probabilities. It is shown that the approach is straightforward to specify distributionally and to imploement computationally, with output readily adopted for constructing required cirterion.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.11a
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• pp.359-362
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• 2003

Carbon fiber sheet is attractive due to its good tensile strength, resistance to corrosion, and low weight. The strengthening of concrete structures with externally bonded carbon fiber sheets is increasingly being used for repair and rehabilitation of existing structures. However CFS strengthened beams break down under the service loads. As rupture strain is not reached ultimate value, reduction of the tensile strength is recommended. This study evaluate CFS tensile strength reduction factor which is required to analyze bending moment.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.8 no.4
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• pp.254-259
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• 1979

Radiation data on south-facing surfaces required for the simplified design pro cedures for solar energy systems, have been computed using the method by Liu and Jordan. For the five localities of South Korea selected from reference 6, the factor R for the conversion of the monthly average daily total radiation on a horizontal surface to that on an inclined surface is presented in Table 5. For the other localities, the convession factor R can by computed using Table 2 through Table 4.

• Journal of Korean Tunnelling and Underground Space Association
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• v.19 no.3
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• pp.409-420
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• 2017

The amount of ventilation required in making the tunnel ventilation plan is an important factor for determining the capacity of the ventilation system. The amount of pollutant emission for each type of vehicle (basic emission amount for the design of ventilation volume) for estimating the required ventilation amount is based on the 'Standard for Allowing the Emission for the car manufacturing', proposed by Ministry of Environment. However, in 2013, the Ministry of Environment announced the 'Regulations on the calculation method of total emissions from vehicles' as a regulation for calculating the pollutants emitted from vehicles. In this regulation, there are the 'Emission factors for each type of vehicle'. Therefore, it is necessary to review the application of the Regulation to the estimation of the required ventilation volume for the road tunnel. In this study, the influence of the strengthened emission regulation in 2015 caused by the case of manipulation of emission volume for the diesel vehicle on the calculation of the required ventilation volume in the road tunnel has been checked. In addition, in this study, the required ventilation volume calculated according to the Standard for Allowing the Emission for the car manufacturing revised by Ministry of Environment and "Emission factors for each type of vehicle" and that calculated according to the EURO emission standard were compared for analysis. This study has implications that it provides the basic design data for calculating the reasonable ventilation capacity of the ventilation system based on the ground for calculating the required ventilation volume.

• Korean Institute of Interior Design Journal
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• v.27 no.1
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• pp.58-67
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• 2018

This study aims to analyze the cases of storage space outside housing unit and propose the activation plan. The issue of storage in residential complex has continued to be reported. This is more salient due to the recent preference trend for small houses, the constant increase of living goods and the limitation of the available space inside housing unit. Although more apartment houses are trying to have individual storage in the exterior space to address these issues, there is still a paucity of the related studies and more studies are required. In this study, we conducted on-site investigation and user interview of each case to propose active usage of the storage space outside housing unit. Result of this study shows that the most crucial factor to improve is 'accessibility' in order to activate the storage space. Storage room should be located close to each household or the individual parking space. The second factor is the size and the structure of the storage room. The sufficient space for keeping and taking large objects out are required. The front of the storage room should have a clogged structure to insure privacy. The third is the provision of suitable support programs such as the system that occupants can check remotely what items are in their storage rooms, garage sale, laundry service, auto cleaning system and rental program. Those programs are required for efficient management and activation of space. Another requirement is to improve environmental factors which include maintenance of optimal temperature and humidity, automatic ventilation system, control of individual lights, and security enhancement. Based on the results of the survey, we provide basic data which can be used for practical space planning to activate exterior storage space of the housing unit. Further surveys are required to develop this study by obtaining more data for generalization.

• Proceedings of the KIEE Conference
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• 2006.04b
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• pp.57-61
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• 2006

In the single-phase switched reluctance motor (SRM) drive, the required DC source is generally supplied by the circuit consisting of bridge rectifier and large filter capacitor connected with DC line terminal. Due to the large capacity of the capacitor, the charged time of capacitor is very short from the AC source. Lead to the bridge rectifiers draws pulsating current from the AC source side, which results in reduction of power factor and low system efficiency. Therefore a novel single-phase SRM drive system is presented in this paper, which includes drive circuit realizing reduction of torque ripple and improvement of power factor with a novel switching topology. The proposed drive circuit consists of one switching part and diode, which can separate the output of AC/DC rectifier from the large capacitor and supply power to SRM alternately, in order to realize the torque ripple reduction and power factor improvement through the switching scheme. In addition, the validity of the proposed method is tested by some simulations and experiments.

• BMB Reports
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• v.29 no.3
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• pp.205-209
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• 1996

To investigate the role of heparin in keratinocyte growth factor (KGF) and acidic fibroblast growth factor (aFGF) high affinity binding to the KGF receptor (KGFR), a cell free system was established which utilized a secreted chimeric molecule between the KGFR extracellular domain and the immunoglobulin heavy chain Fc domain (KGFR-HFc). KGFR-HFc was purified from NIH 3T3 cells and demonstrated the binding of $[^3H]-heparin$ as well as heparin Sepharose. Scatchard analysis showed that the dissociation constant for heparin binding to KGFR-HFc was 140 nM. High affinity KGF and aFGF binding to KGFR-HFc remained unchanged after treatment with 0.6 M NaCl, which is the concentration sufficient to release any bound heparin to the KGFR-HFc. These results strongly suggest that although the KGFR interacts with heparin, the presence of heparin is not absolutely required for high affinity binding of either KGF or aFGF to the KGFR.