• Clinical and Experimental Reproductive Medicine
• /
• 제51권1호
• /
• pp.1-12
• /
• 2024

This review article discusses the integration of artificial intelligence (AI) in assisted reproductive technology and provides key concepts to consider when introducing AI systems into reproductive medicine practices. The article highlights the various applications of AI in reproductive medicine and discusses whether to use commercial or in-house AI systems. This review also provides criteria for implementing new AI systems in the laboratory and discusses the factors that should be considered when introducing AI in the laboratory, including the user interface, scalability, training, support, follow-up, cost, ethics, and data quality. The article emphasises the importance of ethical considerations, data quality, and continuous algorithm updates to ensure the accuracy and safety of AI systems.

• International Journal of Computer Science & Network Security
• /
• 제22권7호
• /
• pp.413-420
• /
• 2022

The review of known methods and decision support systems regarding the possibility of using the reproductive technologies showed that currently there are no methods and decision support systems regarding the possibility of using reproductive technologies taking into account civil law. Although the analyzed methods and systems have great potential for use in different contexts, these methods and systems do not take into account the civil law requirements of any country. The paper has developed a decision support system regarding the possibility of using the reproductive technologies taking into account civil law, which automatically and free of charge determines the possibility/impossibility of surrogate motherhood or in vitro fertilization. If it is determined that surrogate motherhood or in vitro fertilization is impossible, the sufficiency of the information in the analyzed contract is evaluated, and the reasons for the impossibility of surrogate motherhood or in vitro fertilization are presented to the user.

• 한국수정란이식학회지
• /
• 제25권1호
• /
• pp.85-87
• /
• 2010

A necropsy of a primiparous, 4-year-old, Himalayan tahr (Hemitragus jemlahicus) was performed in September 8, 2009. Typical appearances of ovaries, oviducts, uterine horns, cervix and caruncles were well shown. Five cervical folds were present in the cervix. Cervical opening can be reached in the length of less than 15 cm from the entrance of vagina. Development of two follicles was found in the left ovary even though breeding season of this species naturally starts in late November. The reproductive organs of a primiparous, 3-year-old, Corriedale sheep showed that differences in the length and morphological appearance of cervix exist between two species. Comparative understanding of reproductive systems in Himalayan tahr and Corriedale sheep could help advancing assisted reproductive technologies in feral goats.

• Clinical and Experimental Reproductive Medicine
• /
• 제39권1호
• /
• pp.1-9
• /
• 2012

The safety of human exposure to an ever-increasing number and diversity of electromagnetic field (EMF) sources both at work and at home has become a public health issue. To date, many in vivo and in vitro studies have revealed that EMF exposure can alter cellular homeostasis, endocrine function, reproductive function, and fetal development in animal systems. Reproductive parameters reported to be altered by EMF exposure include male germ cell death, the estrous cycle, reproductive endocrine hormones, reproductive organ weights, sperm motility, early embryonic development, and pregnancy success. At the cellular level, an increase in free radicals and $[Ca^{2+}]i$ may mediate the effect of EMFs and lead to cell growth inhibition, protein misfolding, and DNA breaks. The effect of EMF exposure on reproductive function differs according to frequency and wave, strength (energy), and duration of exposure. In the present review, the effects of EMFs on reproductive function are summarized according to the types of EMF, wave type, strength, and duration of exposure at cellular and organism levels.

• Clinical and Experimental Reproductive Medicine
• /
• 제48권2호
• /
• pp.111-123
• /
• 2021

Objective: Using domestic cats as a biomedical research model for fertility preservation, the present study aimed to characterize the influences of ovarian tissue encapsulation in biodegradable hydrogel matrix (fibrinogen/thrombin) on resilience to cryopreservation, and static versus non-static culture systems following ovarian tissue encapsulation and cryopreservation on follicle quality. Methods: In experiment I, ovarian tissues (n=21 animals; 567 ovarian fragments) were assigned to controls or hydrogel encapsulation with 5 or 10 mg/mL fibrinogen (5 or 10 FG). Following cryopreservation (slow freezing or vitrification), follicle viability, morphology, density, and key protein phosphorylation were assessed. In experiment II (based on the findings from experiment I), ovarian tissues (n=10 animals; 270 ovarian fragments) were encapsulated with 10 FG, cryopreserved, and in vitro cultured under static or non-static systems for 7 days followed by similar follicle quality assessments. Results: In experiment I, the combination of 10 FG encapsulation/slow freezing led to greater post-thawed follicle quality than in the control group, as shown by follicle viability (66.9%±2.2% vs. 61.5%±3.1%), normal follicle morphology (62.2% ±2.1% vs. 55.2%±3.5%), and the relative band intensity of vascular endothelial growth factor protein phosphorylation (0.58±0.06 vs. 0.42±0.09). Experiment II demonstrated that hydrogel encapsulation promoted follicle survival and maintenance of follicle development regardless of the culture system when compared to fresh controls. Conclusion: These results provide a better understanding of the role of hydrogel encapsulation and culture systems in ovarian tissue cryopreservation and follicle quality outcomes using an animal model, paving the way for optimized approaches to human fertility preservation.

• Clinical and Experimental Reproductive Medicine
• /
• 제16권2호
• /
• pp.187-204
• /
• 1989

This is a report concerning IVF-ET program and its outcomes of the reproductive center of the Ohio State University(OSU). The pregnancy rate/laparascopy in the OSU reproductive center was average 12.2%. However, the fertilization rate was lower than other reporters. The other problem of the OSU reproductive center was that there was no success in the field of cryopreserved embryo and donor embryo. Therefore, many aspects such as hyperstimulation protocol, culture systems, and embryo transfer technique should have to be reevaluated in order to enhance the outcome of IVF-ET program in the OSU reproductive center.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권1호
• /
• pp.67-71
• /
• 2006

A total of 120 male and 240 female quail (Coturnix coturnix Pharaoh) were used to determine the effect of feeding time on laying and reproductive performance of Pharaoh quail. They were fed ad libitum between 09:00 to 17:00 or full day, daily. Each female-male pair was housed in multiple-bird cages and colony cages. Initial and final body weight, quail-day egg production, feed consumption per egg and mortality were measured to determine laying performance of breeders. A total of 960 eggs were used to determine reproductive performance of quail in each treatment group. Eggs were incubated in a commercial setter and hatcher in standard conditions. Embryonic mortality, apparent fertility, hatchability of total and fertile eggs were calculated to determine the reproductive performance. Results indicated that feeding between 09:00 to 17:00 h reduced final body weight and egg production (p<0.001, p<0.001). Whereas, limited time of feeding improved hatchability of total (p<0.001) and fertile eggs (p<0.001) and reduced embryonic mortality (p<0.001) when compared with the effects of feeding full day. It was found that there were no significant differences for the egg production of quail housed in different cage systems. Quail caged in multiple-bird cages consumed less feed (p<0.01) compared to quail housed in colony cages. There were significant differences for the mortality (p<0.05), hatchability of total (p<0.001) and fertile eggs (p<0.001), and embryonic mortality (p<0.001) during the incubation due to main effect of cage systems. There were significant cage $systems{\times}feeding$ time interactions for hatchability of total and fertile eggs and embryonic mortality (p<0.001). As a conclusion; feeding from 09:00 to 17:00 reduced laying performance of quail and improved the reproductive traits compared to full day feeding of quail breeders. But, further investigations are needed to determine the optimum length of feeding time and egg production of breeders in quail fed limited time must be evaluated in comparison with its beneficial or detrimental effects.

• Toxicological Research
• /
• 제26권4호
• /
• pp.237-243
• /
• 2010

Endocrine disrupting chemicals (EDCs) have been shown to interfere with physiological systems, i.e., adversely affecting hormone balance (endocrine system), or disrupting normal function, in the female and male reproductive organs. Although endocrine disruption is a global concern for human health, its impact and significance and the screening strategy for detecting these synthetic or man-made chemicals are not clearly understood in female and male reproductive functions. Thus, in this review, we summarize the interference of environmental EDCs on reproductive development and function, and toxicological mechanism(s) of EDCs in in vitro and in vivo models of male and female reproductive system. In addition, this review highlights the effect of exposure to multiple EDCs on reproductive functions, and brings attention to their toxicological mechanism(s) through estrogen receptors.

• Clinical and Experimental Reproductive Medicine
• /
• 제47권3호
• /
• pp.180-185
• /
• 2020

Objective: Tyrosine phosphorylation is an essential process in many biological systems, including the male reproductive system. The presence of tyrosine-phosphorylated (TyrPho) proteins has been well documented in male reproductive organs, but research in fertile females is still limited. Methods: The ovary, oviduct, and uterus of adult female Sprague-Dawley rats in the estrus phase were used to localize TyrPho proteins using an immunohistochemical technique. These proteins were separated and their expression patterns were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis, respectively. Results: TyrPho proteins were localized in the cytoplasm of the oocyte except the antral fluid; in the granulosa cells, theca cells, and stromal cells of the ovary; at the apical surface of oviductal epithelial cells; and in the basal epithelium and submucosa of the uterine wall. Moreover, we found that 72-, 43-, and 28-kDa TyrPho proteins were localized in the ovary, while 170-, 55-, and 43-kDa proteins were localized in the oviduct. In the uterus, we detected four major bands, corresponding to 61-, 55-, 54-, and 43-kDa TyrPho proteins. Conclusion: Given that these TyrPho proteins were found in major reproductive organs in the estrus phase, these proteins may play important roles in female fertility.

• BMB Reports
• /
• 제54권5호
• /
• pp.253-259
• /
• 2021

Aging is characterized by a functional decline in most physiological processes, including alterations in cellular metabolism and defense mechanisms. Increasing evidence suggests that caloric restriction extends longevity and retards age-related diseases at least in part by reducing metabolic rate and oxidative stress in a variety of species, including yeast, worms, flies, and mice. Moreover, recent studies in invertebrates - worms and flies, highlight the intricate interrelation between reproductive longevity and somatic aging (known as disposable soma theory of aging), which appears to be conserved in vertebrates. This review is specifically focused on how the reproductive system modulates somatic aging and vice versa in genetic model systems. Since many signaling pathways governing the aging process are evolutionarily conserved, similar mechanisms may be involved in controlling soma and reproductive aging in vertebrates.