• Journal of Genetic Medicine
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• 제6권2호
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• pp.131-145
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• 2009

착상전 유전진단은 유전질환이 이환될 가능성이 있는 부부들을 대상으로 산전진단을 통한 임신중절의 위험성 없이 정상적인 아이를 가질 수 있게 도와주는 보조생식술의 한 방법으로 확립되었다. 단일 할구를 대상으로 하는 분자생물학 및 분자생물학적 기술의 발전은 착상전 유전진단의 정확성을 높은 수준에 이르게 하였고 whole genome amplification 방법을 이용함으로써 단일세포로부터 여러 가지 다양한 진단을 동시에 수행 가능케 하였으며 단일 유전자 질환에 대한 착상전 유전진단에서의 오진을 감소시킬 수 있었다. 따라서 PCR을 이용한 단일 유전자 질환에 대한 착상전 유전진단의 적용가능 유전질환은 더욱 확대될 것이며 건강한 아이의 출산을 원하는 더 많은 부부들에게 기회를 제공해 줄 것이다. 본 종설에서는 현재 단일유전자 질환에 대한 착상전 유전진단을 시행하는 대부분의 센터에서 시행하고 있는 생검 방법과 multiplex PCR, PCR 후 진단 방법, 그리고 multiple displacement amplification 등의 분자생물학적 방법과 단일 세포 분석에서의 문제점 등을 포함한 단일 유전자 질환에 대한 착상전 유전진단 전반에 관하여 논의할 것이다.

• 대한한방부인과학회지
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• 제26권2호
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• pp.46-65
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• 2013

Purpose: Generally menstrual disorder caused by functional deficiency of ovary is not treated clinically because it manifests as normal process of growth. In this study we collected experimental group having several or severe menstrual disorder and we tried to find out whether there is any relationship between menstrual disorders and the function of Thoroughfare and Conception vessels or not. Methods: First, this study was researched to learn the meaning and the symptoms of 'the emaciation of Thoroughfare and Conception vessels', and to learn the relationship between menstrual disorders and 'the emaciation of Thoroughfare and Conception vessels' through literature review. And we set up a experimental group who have two or more menstrual disorders or severe degree of menstrual disorders(N=97) and control group who don't have menstrual disorders(N=97) as a result of the menstruation survey. Afterwards we conducted the DSOM in both experimental group and control group. Results and Conclusions: The meaning of 'the emaciation of Thoroughfare and Conception vessels' is deficiency of qi and blood from the Thoroughfare and Conception vessels. As a result, the female reproductive system is difficult to be worked properly with deficiency of skin, muscles, bone, and vessels. Symptoms of the emaciation of Thoroughfare and Conception vessels are classified into drying, skinny body, feeling of cold, menstrual disorders, pain and personality traits. In this study, severity of drying, skinny body, feeling of cold, menstrual disorders, pain and personality traits is significantly higher in experimental group than in control group. Like this, symptoms of 'the emaciation of Thoroughfare and Conception vessels' appear with menstrual disorders because disorder of circulation is occurred by qi and blood deficiency of Thoroughfare and Conception vessels. It is appropriate to identify this case as 'the emaciation of Thoroughfare and Conception vessels' pattern and necessary to treat actively through regulating qi and blood in advance.

• 정신신체의학
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• 제3권2호
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• pp.115-125
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• 1995

Reports about the prevalence of premenstrual symptoms state that occurs in 20 to 100% of most reproductive-age women. There is a close association between premenstrual syndrome and affective disorders as well as same some other psychiatric disorders. Late luteal phase dysphoric disorder (LLPDD) is a premenstrual condition defined in DSM-III-R by severe mood changes and other symptoms that repeatedly occur only in the luteal phase of the menstrual cycle. However, DSM-III-R does not specify how to compute the change from the follicular to the luteal phase or how to determine when the amount of change is great enough to warrant the diagnosis nor how to determine occupational or social functional impairment. This study was conducted to evaluate the nature, severity and magnitude of premenstrual syndrome in women with current psychiatric disorders by using prospective Daily Rating Form(DRF), and to measure symptom changes according to three scoring methods for diagnosing LLPDD. Our study obtains the data about premenstrual changes estimated by DRF from 22 women with psychiatric disorders who had met criteria for major depressive syndrome on the Premenstrual Assessment Form (PAF). The data was scored by each three methods and was determined to meet criteria A for LLPDD. The results are as follows: 1) the subjects, when scored according to the percent change method, effect size method and absolute severity method, met the DSM-III-R criteria A for LLPDD in 36.4% (8 subjects), 14% (3 subjects) and 4.5% (1 subject) of the cases respectively. 2) The items of irritability, anger and impatience were occurred most frequently on the DRF, when it was scored according to the three scoring methods. And the item of breast pain was next frequent according to the effect size method and the percent change method but according to the absolute severity method. 3) The PAF item of impaired social functioning was reported by 16 (73%) of the subjects. 4) 4 (18%) of the subjects met criteria A for LLPDD and reported impaired social functioning. The prevalency of LLPDD according to each method varied. The percent change method yielded the greatest (36.4%), and the absolute seventy method yielded the laest (4.5%), The effect size method yielded an intermediate frequency (14%). Therefore, for maximizing the diagnostic accuracy of LLPDD, a diagnostic procedure including a measure of change (e.q., effect size method, percent change method) as well as confirmed diagnosis by DRF, will be needed. Also, an accurate tool to evaluate impaired social functioning will be required.

• 대한한방부인과학회지
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• 제25권3호
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• pp.103-116
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• 2012

Objectives: The aim of this study is to differentiate women from men on breast diseases. Methods: We have searched antique books, texts and references of oriental obstetric & gynecology and checked into present conditions of oriental medicine hospitals in republic of Korea. Results: In physiological, pathological and epidemiological viewpoint of both oriental and western medicine, it was accepted that women were different with men on breast disease. Among references published in the Journal of Oriental Obstetrics and Gynecology from 1987, the number of references concerned breast disease was fifty divided into 29 original articles(58%) in types and 26 breast cancer(52%), 13 postpartum lactation(26%) in categorizations. and woman's breast disease and cancer taken charge on oriental obstetrics and gynecology in oriental medicine hospitals were 25(86%) and 19(66%) respectively. Conclusions: We could not realize that woman's breast disease already were accepted woman's particularity in various aspects until conducting our study. Accordingly, it is suggested that more researches would be conducted on the needs of female patients with breast disease through questionnaire or research and on the association reproductive factor and breast disease of women.

• 대한수의학회지
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• 제54권4호
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• pp.219-224
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• 2014

Uterine sterilization is important for improving fertility in cattle. This study compared bacterial flora in the uterus between healthy and repeat breeder cows (RBCs). The uterine flushing of six heifers, 13 healthy HanWoo cows and eight RBCs (HanWoo) were sampled, and 15 frozen semen samples were selected. Overall, 35 bacteria were identified from in HanWoo uterine flushing and semen. The bacterial genera identified from HanWoo uterine flushing were Alloiococcus, Bacillus, Enterobacter, Enterococcus, Erysipelothrix, Gardnerella, Granulicatella, Kocuria, Pantoea, Pasteurella, Rothia, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The bacterial genera identified from HanWoo semen were Bacillus, Escherichia, Kocuria, Oligella, Pseudomonas, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The prevalence and presence of the identified bacteria between healthy cows and RBCs differed significantly. Further studies are needed to determine the role of these bacteria in the uterus of HanWoo cattle with reproductive disorder.

• 한국가축번식학회지
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• 제12권3호
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• pp.156-161
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• 1988

Holstein cows being raised in the Kangweon district were examined on the values of hematology in infertile cows. The results obtained were as follows; 1. Erythrocyte count was 6.23$\pm$1.49$\times$106/㎣ 2. Hemoglobin was 10.6$\pm$2.00g/100ml 3. Hematocrit was 29.68$\pm$3.27ml/100ml 4. Mean corpuscular volume was 49.4$\pm$12.1$\mu$3 5. Mean corpuscular hemoglobin was 17.3$\pm$3.73pg 6. Mean corpuscular hemoglobin concentration was 35.7$\pm$6.37% 7. Thrombocytes were 324.06$\pm$88.11$\times$103/㎣ 8. Total leukocytes was 8.70$\pm$2.23$\times$103/㎣ 9. Neutrophils, lymphocytes, monicytes, eosiniohils and basophis were 34.44$\pm$6.39, 53.82$\pm$5.84, 2.17$\pm$2.18, 9.10$\pm$3.85 and 0.50$\pm$1.80%, respectively.

• 한국가축번식학회지
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• 제12권3호
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• pp.162-166
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• 1988

Holstein cows being raised in the Kangweon district were examined on the values of blood chemistry in infertile cows. The results obtained were as follows; 1. Total serum protein was 7.52$\pm$0.45g/100ml 2. Serum albumin was 3.06$\pm$0.28g/100ml 3. In values of serum globulin, $\alpha$1-globulin, $\alpha$2- globulin, $\beta$-globulin and ${\gamma}$-globulin were 0.79$\pm$0.14, 0.60$\pm$0.10, 0.86$\pm$0.13 and 2.21$\pm$0.35ng/100ml, respectively. 4. A/G ration was 0.69$\pm$0.09 5. Total cholesterol in serum was 119.73$\pm$34.52mg/100ml 6. Blood glucose was 55.24$\pm$6.46mg/100ml 7. Serum calcium was 9.27$\pm$0.87mg/100ml 8. Serum inorganic phosphorus was 5.74$\pm$0.86mg/100ml 9. Serum glutamic oxalacetic transaminase was 47.90$\pm$11.02I$\mu$/l 10. Serum glutamic pyruvic transaminase was 14.20$\pm$3.02I$\mu$/l

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7011-7014
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• 2014

The polycystic ovary syndrome is the most common endocrinological disorder of reproductive age women with a prevalence of 5 to 8 %. The most common diagnostic criteria used for polycystic ovary syndrome are oligo- or an-ovulation, clinical and/ or biochemical signs of hyperandrogenism and polycystic ovaries. Hyperandrogenism results in increased estrogen levels and lack of cyclic progesterone due to anovulation and persistent stimulation of the endometrium may lead to endometrial hyperplasia or adenocarcinoma development. In this mini review, we aimed to evaluate the possible relationship between polycystic ovary syndrome and endometrial cancer.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 1998년도 제4차 학술발표대회 및 정기총회
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• pp.50-51
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• 1998

guanidinoacetate methyltransferase (GAMT) catalyzes the last step of creatine biosynthesis in mammals. Creatine plays an important role in cellular energy metabolism in variety of tissues including brain and male reproductive tract. Congenital deficiency of the enzyme leads to a neurologic disorder in humans. We used an interspecific backcross DNA panel to map Gamt to the central region of mouse Chromosome (Chr) 10 near the locus of hesitant mutation affecting male fertility. We assigned the human GAMT gene to Chr 19 by PCR analysis of a human/rodent somatic hybrid cell line DNA panel, and further localized the human gene to Chr 19 at band p13.3 by PCR analysis of a human radiation hybrid DNA panel. Human chr 19p13.3 is homologous to the central part of mouse Chr 10 where mouse Gamt is located. Furthermore, this part of mouse Chr 10 contains mutant loci the phenotype of which is similar to the GAMT deficiency in human.

• 한국동물위생학회지
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• 제40권2호
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• pp.133-137
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• 2017

Three swine farms which were suffering from slight economic loss due to suckling piglets' diarrhea, were selected to apply commercialized multivalent vaccine for sow use; $SUISENG^{(R)}$ (Hipra, Spain). Farms were pre-diagnosed with clinical symptoms and molecular detection of C. perfringens Type A and C and E. coli pili by PCR. Sows were vaccinated twice 2 ml of the vaccine at 6 and 3 weeks ante partum intramuscularly according to the manufacturer's instruction. All vaccinated sows did not show any adverse reaction or clinical signs; hypersensitivity, fever, granuloma or abscess on the injection site, appetite loss, and so on. Also, no reproductive disorder was appeared in vaccinated sows compared with non-vaccinated control sows. The results suggested that piglets born from vaccinated sows show significantly better performance in regard of the diarrhea index and mean daily weight gain compared with piglets from non-vaccinated sows. Therefore, the commercial vaccine for the prevention of neonatal diarrhea is found to be effective in reducing diarrhea in the first suckling period of piglets after birth.