• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.106-106
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• 2017

The soybean [Glycine max (L.) Merr.] is one of the most important crop resources worldwide as food and forage. It is also important and valuable that to hold crop resources to have high genetic diversities. Recently, a core collection has been constructed in many plants to preserve the genetic resources of various plants. A core collection is small population to represent the genetic diversity of the total collection, and is of strategic importance as they allow the use of a small part of a germplasm collection that is representative of the total collection. Here, we developed the core collection consisting of 816 accessions by using approximately 180,000 (180K) single nucleotide polymorphisms (SNPs) developed in previous study. In addition, we performed genetic diversity and population structure analysis to construct the core collection from entire 4,392 collections. there were excluded sample call rates less than 93% and duplicated samples more than 99.9% according to genotype analysis using 180K SNPs from entire collections. Furthermore, we were also excluded natural hybrid resources which Glycine max and Glycine soja are mixed in half through population structure analysis. As a result, we are constructed the core collection of genetic diversity that reflects 99% of the entire collections, including 430 cultivated soybeans (Glycine max) and 386 wild soybeans (Glycine soja). The core collection developed in this study should be to provide useful materials for both soybean breeding programs and genome-wide association studies.

• Journal of Korean Society of Rural Planning
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• v.21 no.4
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• pp.157-165
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• 2015

The purpose of this study is on identifying charactreistics and management direction of complementary pond of Dalmaji village in Gangjin. Accordingly, this village was indicated as Experience and Resort village by Korean government. This study describes the construction background and characteristics and identifies species diversity and characteristics through field survey. For this, literature and field survey was performed. In field survey, vegetational survey was performed in Aug. of 2015. D(Dominant degree) and S(Sociability degree) was measured by Brown-Blanquet's method. The answer was searched from Myungsanron(明山論), a textbook of Fengsui(風水) in Joseon dynasty. Of these causes, it was considered that the representative one is a protection of fire and infectious disease because the Wolgak mountain is a fire mountain(火山) as a stone mountain in Fengsui. The results demonstrate decline of aquatic ecosystem because of neglect management. The total number of species was 24. Of the species, Zelkova serrata is a natural vegetation of this complementary forest. This is nominated as Lagerstroemia indica and Camellia japonica community. Species composition demonstrate the characteristics of Korean southern region as mixed forest of evergreen trees and deciduous ones which are indigenous plants. It was considered Juniperus chinensis KaizukaVariega in central round island was planted in Japanese colonial period and it have to be removed. It was considered that name of moon pond and moon island etc. projects the mind of village ancestors which are going to reach an ideal condition of moon. And management of quantity and depth of pond water was critical for restoration of disappearing lotus from related literature. Periodic management was needed for ecological structure and function improvement because of ecological disturbance by the invasive plants like Humulus japonicus.

• Analytical Science and Technology
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• v.30 no.6
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• pp.379-389
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• 2017

In this study, we set up an analytical method that can be used for rapid and accurate determination of representative isoquinoline alkaloids in medicinal plants using UPLC-ESI-Q-TOF MS (ultra pressure liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry). The compounds were eluted on a C18 column with 0.1 % formic acid and acetonitrile, and separated with good resolution within 13 min. Each of the separated components was characterized by precursor ions (generated by ESI-Q-TOF) and fragment ions (produced by collision-induced dissociation, CID), which were used as a reliable database. We also performed method validation: analytes showed excellent linearity ($R^2$, 0.9971-0.9996), LOD (5-25 ng/mL), LOQ (17-82 ng/mL), accuracy (91.6-97.4 %) as well as intra- and inter-day precisions (RSD, 1.8-3.2 %). In the analysis of Chelidonium majus L., magnoflorine, coptisine, sanguinarine, berberine and palmatine were detected by matching retention times and characteristic fragment ion patterns of reference standards. We also confirmed that, among the quantified components, coptisine was present in the highest quantity. Furthermore, alkaloid profiling was carried out by analyzing the fragment ion patterns corresponding to peaks of unknown components. In this manner, protopine, chelidonine, stylopine, dihydroberberine, canadine, and nitidine were tentatively identified. We also proposed the molecular structure of the fragment ions that appear in the mass spectrum. Therefore, we concluded that our suggested method for the determination of major isoquinoline alkaloids by UPLC-Q-TOF can be useful not only for quality control, but also for rapid and accurate investigation of phytochemical constituents of medicinal plants.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.452-457
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• 1998

Thirty-eight isolates of Phytophthora sp. caused rots on roots and basal stems were collected from five flowering plants from 1992 to 1997 at eight cultivation areas in Korea. All the isolates were identified as P. nicotianae based on following characteristics. The fungus produced markedly papillate, not caducous and ovoid to spherical sporangia, abundant chlamydospores, and small oospores with amphigynous antheridia only when paired with either A1 or A2 mating type. All isolates grew well at 35$^{\circ}C$ and showed distinct arachnoid colony patterns on CMA and PDA. Sizes of sporangia and chlamydospores of five representative isolates from each plant averaged 43-52$\times$30-38 ${\mu}{\textrm}{m}$ and 28 ~34 ${\mu}{\textrm}{m}$. Mating type of the isolates was either A1 or A2, and oogonia and oospores were measured as 28~31 ${\mu}{\textrm}{m}$ and 21~25 ${\mu}{\textrm}{m}$. PCR-RFLP analysis of rDNA of the five isolates resulted that restriction band patterns of the small subunit and ITS regions were identical to a perilla isolate of P. nicotianae, but distinct from P. cactorum and P. capsici. Cross inoculation tests showed that the five isolates had pathogenicity to lily, christmas cactus, anthurium, baby's breath and carnation with different degrees. However, each isolate showed stronger pathogenicity to its corresponding original host than others. Among five lily cultivars Georgia and Quririna were more susceptible than Napoli and others. This is first report of Phytophthora root and stem rot of lily, Christmas cactus, anthurium, baby's breath and monochoria in Korea.

• Nuclear Engineering and Technology
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• v.47 no.1
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• pp.26-32
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• 2015

During the course of a severe accident in a light water nuclear reactor, large amounts of hydrogen can be generated and released into the containment during reactor core degradation. Additional burnable gases [hydrogen ($H_2$) and carbon monoxide (CO)] may be released into the containment in the corium/concrete interaction. This could subsequently raise a combustion hazard. As the Fukushima accidents revealed, hydrogen combustion can cause high pressure spikes that could challenge the reactor buildings and lead to failure of the surrounding buildings. To prevent the gas explosion hazard, most mitigation strategies adopted by European countries are based on the implementation of passive autocatalytic recombiners (PARs). Studies of representative accident sequences indicate that, despite the installation of PARs, it is difficult to prevent at all times and locations, the formation of a combustible mixture that potentially leads to local flame acceleration. Complementary research and development (R&D) projects were recently launched to understand better the phenomena associated with the combustion hazard and to address the issues highlighted after the Fukushima Daiichi events such as explosion hazard in the venting system and the potential flammable mixture migration into spaces beyond the primary containment. The expected results will be used to improve the modeling tools and methodology for hydrogen risk assessment and severe accident management guidelines. The present paper aims to present the methodology adopted by Institut de Radioprotection et de $S{\hat{u}}ret{\acute{e}}$ $Nucl{\acute{e}}aire$ to assess hydrogen risk in nuclear power plants, in particular French nuclear power plants, the open issues, and the ongoing R&D programs related to hydrogen distribution, mitigation, and combustion.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.3
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• pp.33-48
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• 2009

A palustrine wetland is a type of a wetland that prevails in Korea as well as an ecosystem that provides various ecological functions and has affluent biodiversity. This study was conducted to understand the characteristics of vegetation in a palustrine wetland by analyzing a life form and naturalized plants, in order to present the maintenance and utilization plan of a small wetland. A total of 249 taxa including 76 families, 188 generics, 209 species, 38 varieties and 2 forma were found at six investigated wetlands. As a result of the appearance ratio analysis, Therophytes (37.8%) with 94 taxa and Hemicryptophytes (31.3%) with 78 taxa were in order of life form, and Gramineae (14.1 %) with 35 taxa and Compositae (11.2 %) with 28 taxa were in order of family level. These were deemed representative vegetation in a palustrine wetland. Equosetum arvense, Phragmites communis, Commelina communis, Persicaria thunbergii, Arenaria serpyllifolia, Stellaria media, Erigeron annuus, Erigeron canadensis and Artemisia princeps var, orientalis were found continuously for a period of investigation in all sites. Equosetum arvense, Phragmites communis and Persicaria thunbergii were species with high appearance frequency. Especially, Persicaria thunbergii was construed high in a cover ratio in May as it develop first among Hydrophytes and form a canopy sooner than anything else. We found that a degree of naturalization and an urbanization index appear comparatively high in Yeongok-ri, Cheonan, Gangjang-ri, Asan and Yodang-ri, Hwasung, which is construed due to artificial interference, such as fanning, fishing, swimming and green-tour program. These results would be used to utilize in restoring a deserted and damaged wetland and to provide fundamental data in creating a wetland as follows.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.5
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• pp.724-734
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• 2019

This study selected a representative small arm firing range and analyzed the distribution of heavy metal pollutants such as Pb, Cu, Zn, etc. For this the concentrations of heavy metals in soils, roots and leaves of plants, and water of the small arm firing range were measured. The concentrations of heavy metals in the effluent were also checked during precipitation. The concentration of lead in the samples collected from the top soil(0-5 cm) and sub soil(5-50 cm) near the target in the small arm firing range exceeded the concern level of the Soil Environment Conservation Act of Korea, but not in other soil samples. Plants that grow in soil heavily contaminated with lead showed a high lead concentration, especially in roots. However, the concentration of lead in effluent from the small arm firing range was less than 0.02 ppm. The concentration of copper and zinc in the small arm firing range did not surpass the concern level of the Soil Environment Conservation Act of Korea. Through this study more accurate information on the distribution of heavy metal pollution in the soil of the small arm firing range was obtained. Based on this research, we can conclude that some facility improvements can reduce the spreading of pollutants in the currently used small arm firing range and contribute to the design and operation of advanced small arm firing ranges.

• Nuclear Engineering and Technology
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• v.51 no.6
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• pp.1708-1714
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• 2019

The purpose of the critical evaluation of the spent fuel pool (SFP) is to verify that the maximum effective multiplication factor ($K_{eff}$) is less than the critical safety limit at 100% stored condition of the spent fuel with the maximum reactivity. At nuclear power plants, the storage standard of spent fuel, ie, the loading curve, is established to prevent criticality from being generated in SFP. Here, the loading curve refers to a graph showing the minimum discharged burnup versus the initial enrichment of spent fuel. Recently, US NRC proposed the new critical safety assessment guideline (DSS-ISG-2010-01, Revision 0) of PWR SFPs and most of utilities in US is following it. Of course, the licensed criterion of the maximum effective multiplication factor of SFP remains unchanged and it should be less than 0.95 from the 95% probability and the 95% confidence level. However, the new guideline is including the new evaluation methodologies like the application of the axial burnup profile, the validation of depletion and criticality code, and trend analysis. Among the new evaluation methodologies, the most important factor that affects $K_{eff}$ is the axial burnup profile of spent fuel. US NRC recommends to consider the axial burnup profiles presented in NUREG-6801 in criticality analysis. In this paper, criticality effect was evaluated considering three profiles, respectively: i) Axial burnup profiles presented in NUREG-6801. ii) Representative PWR axial burnup profile. iii) Uniform axial burnup profile. As the result, the case applying the axial burnup profiles presented in NUREG-6801 showed the highest $K_{eff}$ among three cases. Therefore, we need to introduce a new methodology because it can be issued if the axial burnup profiles presented in NUREG/CR-6801 are applied to the domestic nuclear power plants without any other consideration.

• Journal of the Earthquake Engineering Society of Korea
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• v.25 no.6
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• pp.261-266
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• 2021

Nuclear power plants in Korea were designed and evaluated based on the NRC's Regulatory Guide 1.60, a design response spectrum for nuclear power plants. However, it can be seen that the seismic motion characteristics are different when analyzing the Gyeongju earthquake and the Pohang earthquake that has recently occurred in Korea. Compared to the design response spectrum, seismic motion characteristics in Korea have a larger spectral acceleration in the high-frequency region. Therefore, in the case of equipment with a high natural frequency installed in a nuclear power plant, seismic performance may be reduced by reflecting the characteristics of domestic seismic motions. The failure modes of the equipment are typically structural failure and functional failure, with an anchorage failure being a representative type of structural failure. In this study, comparative analyses were performed to decide whether to consider the inelastic behavior of the anchorage or not. As a result, it was confirmed that the seismic performance of the anchorages could be increased by considering the inelastic behavior of an anchorage.

• Korean Journal of Plant Resources
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• v.32 no.5
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• pp.565-588
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• 2019

This study aimed to understand current flora of Western Civilian Control Zone (CCZ) in Korea. Some areas that located at Paju-si and Yeoncheon-gun were selected as representative survey area (Jangdan-myeon, Deokjin mountain fortress, Ilwol peak of Mt. Seokbyeong, Imjinriver, Sunaecheon stream, Sewolcheon stream in Paju and Banjeong-ri in Yeoncheon). Through this survey, 461 vascular plant specimens were collected from April 2012 to September 2014, twice a month and from October 2014 to October 2018, once a month. These were finally classified into 96 families 305 genera 413 species 4 subspecies 41 varieties 6 forms totally 464 taxa. There are remarkable plants such as 6 taxa of Korean endemic species, 44 taxa of specified species on a floristics aspect, and 35 taxa of alien and naturalized plants (7.5%). Meanwhile, it has not been observed any endangered plant species during the activities in this area.