• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.2028-2036
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• 2017

The Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts for scientific research and biotechnological applications. However, omics analyses have revealed that E. coli K-12 and B exhibit notably different genotypic and phenotypic attributes, even though they were derived from the same ancestor. In a previous study, we identified a limited number of proteins from the two strains using two-dimensional gel electrophoresis and tandem mass spectrometry (MS/MS). In this study, an in-depth analysis of the physiological behavior of the E. coli K-12 and B strains at the proteomic level was performed using six-plex isobaric tandem mass tag-based quantitative MS. Additionally, the best lysis buffer for increasing the efficiency of protein extraction was selected from three tested buffers prior to the quantitative proteomic analysis. This study identifies the largest number of proteins in the two E. coli strains reported to date and is the first to show the dynamics of these proteins. Notable differences in proteins associated with key cellular properties, including some metabolic pathways, the biosynthesis and degradation of amino acids, membrane integrity, cellular tolerance, and motility, were found between the two representative strains. Compared with previous studies, these proteomic results provide a more holistic view of the overall state of E. coli cells based on a single proteomic study and reveal significant insights into why the two strains show distinct phenotypes. Additionally, the resulting data provide in-depth information that will help fine-tune processes in the future.

• Korean Journal of Organic Agriculture
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• v.17 no.4
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• pp.413-439
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• 2009

This study conducts a survey and test to understand U.S. public's perception about multifunctionality. The questionnaire suggests seven alternative way of providing questions about intangible benefits provided by agriculture in the U.S. The final questionnaire was administered as an e-mail survey in June 2008 to a nationally representative household panel maintained in the U.S. by the Ipsos Observer. Data analysis shows that 64 percent of respondents considered the multifunctionality of agriculiture as an important issue and 45 percent of respondents were in favor of increasing government expenditure to support farmland preservation. Using Fishbein's multi-attribute model as a theoretical background, this paper develops an empirical model to assess and attributes of multifunctionality. For the analysis, bivariate orderd probit model was set up to reflect respondent's attitude. Regression analyses show that two questions (how much you agree with agriculture's intangible benefit and increasing government expenditure to support agriculture) are shaped by different sets of facts.

• Journal of Engineering Education Research
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• v.19 no.2
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• pp.60-69
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• 2016

This paper deals with the selection method of effective engineering design problem based on LEGO Mindstorm NXT for basic design education. By YouTube case study of various LEGO-based engineering designs for olympic sports, performance criteria have been developed including programming complexity, structural complexity, sensor/actuator complexity and variety of game operation. Programming complexity includes range of programming code length and possible program variety. Structural complexity includes variety of structural elements such as length, shape, weight, and volume to overcome design restrictions. Sensor/actuator complexity includes variety of sensor used and number of possible actuator assemblies. Variety of game operation includes game complexity and required creativity to make LEGO robots. Based on these performance criteria, four representative sports were selected as the candidates for effective engineering design problem. Finally, feasibility and attributes of each candidate were verified by real implementation examples.

• Korean journal of food and cookery science
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• v.9 no.2
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• pp.99-104
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• 1993

This study was carried out in order to develop supplementary foods for infants using Korean foods. Thirty-four different kinds of supplementary foods were developed and fourteen representative ones were selected to be analyzed chemically. The results are as follows: 1. The developed supplementary foods were 34 kinds and divided into 3 stages. First stage is designed for the babies just beginning to eat pureed vegetables and fruits. Second stage combined the nutritional attributes of both vegetables and meat. Third stage featured tender, bite-size pieces of meats and vegetables that appealed to the most mature tastes of basies. 2. In the production of prepared foods; water, milk, vegetable juice, fruit juice, and soy milk were used as the liquid source; rice, rice starch, chestnut, noodle, potatoes, sweet potatoes, rice cakes as the carbohydrate source; fish, meats soybean curd, beans, eggs, chicken, cow liver as the protein source; and vegetables and fruits were used as vitamin & mineral source. 3. The approximate composition range of the products were 10.91∼24.46% carbohydrate, 0.15∼6.06% protein, 0.092∼7% fat, 0.13∼ 1.37% ash, 0.63∼36.34% calcium, 0.092∼0.48% iron and 0.42∼16.36% vitamin C.

• Journal of Korea Game Society
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• v.17 no.2
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• pp.7-16
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• 2017

In this paper, it is suggested that the elements constituting the gay empire are similar to the main pedagogical concept of John Dewey, a representative educational philosopher. I analyzed some factors that make up the concept of gamification by connecting them with the following five attributes. Continuity, interaction, interest, growth, and educational experience are the main factors that contribute to sustainability. Based on the theoretical analysis of the main concept, I try to find out the relation between the game and education, and understand the meaning of the gamification, not the game.

• The Journal of Information Systems
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• v.29 no.1
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• pp.1-22
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• 2020

Purpose Along with increasing use of mobile devices, the sharing economy platform services, which generate profits by sharing products owned or labor, have been activated. A representative example of the sharing economy platform service is Airbnb, which connects providers having idle residential space with users. The results revealed that the cases of superhosts and professional companies had a positive and negative effect on user satisfaction, respectively. Based on signaling theory, this study drew the following implications: residential space providers should make an effort to meet the superhost conditions suggested by Airbnb, and offering residential spaces by individual suppliers rather than professional accommodation companies can more heighten user satisfaction. Design/methodology/approach Because sharing behavior is promoted through trust between interested parties, this study aimed to verify the effects of provider characteristics on user satisfaction in a sharing economy platform service. Specifically, it analyzed user satisfaction, according to host attributes (i.e., "superhost or not," gender, and "professional company or not") among the characteristics of Airbnb suppliers. Findings The results revealed that the cases of superhosts and professional companies had a positive and negative effect on user satisfaction, respectively. Based on these analyses results, this study drew the following implications: residential space providers should make an effort to meet the superhost conditions suggested by Airbnb, and offering residential spaces by individual suppliers rather than professional accommodation companies can more heighten user satisfaction.

• The Journal of the Korea institute of electronic communication sciences
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• v.10 no.2
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• pp.219-226
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• 2015

As a representative system of the 4th generation mobile communication system, LTE provides much higher transmission rate than the former ones. Considering the varying property that a wireless environment shows, the transmission rate is influenced very deeply by the wireless channel conditions. The transmission rate is one of the most important attributes that has a primary effect on user's satisfaction of the service provided through the LTE system. After we have selected a few metrics that indicate the wireless channel quality, therefore, we have conducted a measurement for the metrics over the real commercial LTE system. By analyzing the measured results, we have found that the channel quality affect more on the transmission rate as the channel quality becomes poorer.

• Journal of Korean Society of Rural Planning
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• v.16 no.2
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• pp.1-10
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• 2010

This study aimed to systematically adjust rural area development projects that have been carried out by a variety of government departments for activation of rural areas and overcome inefficient problems caused by the policies and projects promoted separately. As its alternative, this study aimed to explore efficient improvement scheme of rural villages associated with various projects. Major agriculture/rural policies and projects that have been promoted were classified by the target space of the policies and projects as Environment space, living space, and production space. Each space was categorized into five sub-items without duplication. By assessment results of rural area improvement level and preference by Analytic Hierarchy Process(AHP) method, maintenance of rural areas was classified into four types. And outer space structure was analyzed by using Space Syntax Method(SSM). Based on the analysis result above, facilities for improvement of rural villages were placed around representative facilities. Facility allocation was based on selection and concentration in terms of facility maintenance and on type attributes in terms of spatial aspects. And finally, alternatives schemes for setting up the basic direction of improvement of rural villages are local area characteristics and environmentally conscious business plan.

• English & American cultural studies
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• v.18 no.1
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• pp.93-120
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• 2018

Constance Fenimore Woolson is among those whom scholars have for long been trying to rediscover and add to the list of representative American writers. The primary methodology has been regionalism, based on the fact that most of her work portrays remote, exotic regions in and out of America. Still, Woolson remains obscure to general readers as well as literary critics outside a small circle of her scholarship. This essay attributes that obscurity to Woolson scholars' blind reliance on regionalism's nationalistic assumption in reading Woolson's multifaceted writing, and proposes to explore her nationally and regionally displacing view of the rigidly stereotypical and ideologically biased binary opposition between the center and the margin in postbellum America. The essay takes as an example the only story by Woolson that has never been reprinted or anthologized until very recently, "In Sloane Street," and examines why it resists the scholarly endeavor to regional categorization. The examination especially focuses on how the story exposes the Americanizing conceptualization of the region and its limits. The essay concludes with an attention to the story's ending where Woolson abruptly yet deliberately introduces a form of almanac as the main character Gertrude's mode of daily record. The attention to that uniquely hybrid genre in the American literary tradition, which encompasses the public and the private, the universal and the local, sheds light on Woolson's authorial intention to deconstruct the Manichean view of literary regionalism.

• Journal of Korea Game Society
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• v.21 no.6
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• pp.97-110
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• 2021

The purpose of this study is to analyze how video media such as movies and dramas, which aim for one-way and viewing-oriented content, remediates the attributes of games, and to study the influence and convergence of games and video media. There were two main types of game culture convergence patterns of video media. The first is the passive fusion of the content aspect of the game, and the second is the active fusion of the system aspect of the game. Such research is significant as a basic study that reveals that games affect various video media and proves that game media can function as a representative media leading popular culture.