• Title/Summary/Keyword: repetitive structure

Search Result 207, Processing Time 0.027 seconds

Structure and Diversity of Arsenic-Resistant Bacteria in an Old Tin Mine Area of Thailand

  • Jareonmit, Pechrada;Sajjaphan, Kannika;Sadowsky, Michael J.
    • Journal of Microbiology and Biotechnology
    • /
    • v.20 no.1
    • /
    • pp.169-178
    • /
    • 2010
  • The microbial community structure in Thailand soils contaminated with low and high levels of arsenic was determined by denaturing gradient gel electrophoresis. Band pattern analysis indicated that the bacterial community was not significantly different in the two soils. Phylogenetic analysis obtained by excising and sequencing six bands indicated that the soils were dominated by Arthrobacter koreensis and $\beta$-Proteobacteria. Two hundred and sixty-two bacterial isolates were obtained from arsenic-contaminated soils. The majority of the As-resistant isolates were Gramnegative bacteria. MIC studies indicated that all of the tested bacteria had greater resistance to arsenate than arsenite. Some strains were capable of growing in medium containing up to 1,500 mg/l arsenite and arsenate. Correlations analysis of resistance patterns of arsenite resistance indicated that the isolated bacteria could be categorized into 13 groups, with a maximum similarity value of 100%. All strains were also evaluated for resistance to eight antibiotics. The antibiotic resistance patterns divided the strains into 100 unique groups, indicating that the strains were very diverse. Isolates from each antibiotic resistance group were characterized in more detail by using the repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique with ERIC primers. The PCR products were analyzed by agarose gel electrophoresis. The genetic relatedness of 100 bacterial fingerprints, determined by using the Pearson product-moment similarity coefficient, showed that the isolates could be divided into four clusters, with similarity values ranging from 5-99%. Although many isolates were genetically diverse, others were clonal in nature. Additionally, the arsenic-resistant isolates were examined for the presence of arsenic resistance (ars) genes by using PCR, and 30% of the isolates were found to carry an arsenate reductase encoded by the arsC gene.

Design of a Fuzzy Classifier by Repetitive Analyses of Multifeatures (다중 특징의 반복적 분석에 의한 퍼지 분류기의 설계)

  • 신대정;나승유
    • Journal of the Korean Institute of Intelligent Systems
    • /
    • v.6 no.3
    • /
    • pp.14-24
    • /
    • 1996
  • A fuzzy classifier which needs various analyses of features using genetic algorithms is proposed. The fuzzy classifier has a simple structure, which contains a classification part based on fuzzy logic theory and a rule generation ation padptu sing genetic algorithms. The rule generation part determines optimal fuzzy membership functions and inclusior~ or exclusion of each feature in fuzzy classification rules. We analyzed recognition rate of a specific object, then added finer features repetitively, if necessary, to the object which has large misclassification rate. And we introduce repetitive analyses method for the minimum size of string and population, and for the improvement of recognition rates. This classifier is applied to three examples of the classification of iris data, the discrimination of thyroid gland cancer cells and the recognition of confusing handwritten and printed numerals. In the recognition of confusing handwritten and printed numerals, each sample numeral is classified into one of the groups which are divided according to the sample structure. The fuzzy classifier proposed in this paper has recognition rates of 98. 67% for iris data, 98.25% for thyroid gland cancer cells and 96.3% for confusing handwritten and printed numeral!;.

  • PDF

A Study on the Deflection of Roller Axis of an Improved Mecanum Wheel Using the Castigliano Theorem (카스틸리아노 정리를 이용한 개선된 메카넘 휠의 롤러 축의 처짐에 대한 연구)

  • Hwang, Hui-Geon;Chung, Won-Jee;Kim, Dae-Young
    • Journal of the Korean Society of Manufacturing Process Engineers
    • /
    • v.19 no.6
    • /
    • pp.61-66
    • /
    • 2020
  • For simple and repetitive transport tasks in limited spaces such as in factories, it is more efficient to use mobile robots instead of human workers. For this reason, the reliance on mobile robots is increasing due to the increased implementation of smart factories. Currently, the structural design of the Mecanum wheel is required to ensure the stability of the moving robot since it is used for the transport of not only small products but also large products. In this paper, to improve the stability and durability of the Mecanum wheel, ways to improve the structure of the Mecanum wheel are presented. Then, using the Castigliano theorem, the structural stability is reviewed through the deflection on existing and improved structures.

Effectiveness of Intensive Versus Extensive Reading of Children's Stories (동화의 정독적 반복 들려주기와 다독적 한번 들려주기에 따른 유아의 흥미도와 이야기 구조화)

  • Shon, Hye Sook
    • Korean Journal of Child Studies
    • /
    • v.25 no.2
    • /
    • pp.59-69
    • /
    • 2004
  • The question addressed by the present research was whether repeated, careful reading of a few stories(Intensive Reading) is more effective than general, one-time reading of many stories(Extensive Reading). Unfamiliar stories were read to 105 kindergarten children from typewritten sheets of paper for 40 days(about 10 weeks). The Intensive Reading group heard 5 stories and the Extensive Reading group heard 40 stories. Effectiveness was measured by interest level during story telling and by narrative structure. Test scores were analysed by ANCOVA. No differences were found between the 2 groups; however, as a new area of study it could serve as a catalyst for research on story telling methods.

  • PDF

A new ALE formulation for sloshing analysis

  • Aquelet, N.;Souli, M.;Gabrys, J.;Olovson, L.
    • Structural Engineering and Mechanics
    • /
    • v.16 no.4
    • /
    • pp.423-440
    • /
    • 2003
  • Arbitrary Lagrangian Eulerian finite element methods gain interest for the capability to control mesh geometry independently from material geometry, the ALE methods are used to create a new undistorted mesh for the fluid domain. In this paper we use the ALE technique to solve fuel slosh problem. Fuel slosh is an important design consideration not only for the fuel tank, but also for the structure supporting the fuel tank. "Fuel slosh" can be generated by many ways: abrupt changes in acceleration (braking), as well as abrupt changes in direction (highway exit-ramp). Repetitive motion can also be involved if a "sloshing resonance" is generated. These sloshing events can in turn affect the overall performance of the parent structure. A finite element analysis method has been developed to analyze this complex event. A new ALE formulation for the fluid mesh has been developed to keep the fluid mesh integrity during the motion of the tank. This paper explains the analysis capabilities on a technical level. Following the explanation, the analysis capabilities are validated against theoretical using potential flow for calculating fuel slosh frequency.

Electrochemical Property Measurement on Flyacenic Semiconductor(PAS) (PAS 전극에 관한 전기화학적 특성 측정)

  • 김한주;박수길;손원근;이홍기;이주성
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 1999.05a
    • /
    • pp.197-200
    • /
    • 1999
  • The polyacene materials prepared from phenol resine at relatively low temperature(550~75$0^{\circ}C$) show a highly Li-doped state up to $C_2$Li state without liberation of Li cluster. We prepared each polyacenic materials various temperature and investigated electrochemical property. We tried to change the mole ratio of [H]/[C] that was 0.24~0.4 range and finally found that the further discussion of improvements of battery materials. The X-ray structural analyses have shown that this material is essentiallly amorphous with loose structure in molecular size order. This structure ensures that the PAS battery has both reliability on repetitive doping-undoping processes and higher energy density than other batteries. The PAS electrode has been confirmed to show good stability and reversibility.

  • PDF

Application of Geotextile to ensure the Motor Pouring Ability for Paved Track (포장궤도 모르터 충진성능 확보를 위한 토목섬유의 적용)

  • Lee Ho-Ryong;Park Jong-Kyan;Hwang Seon-Keun;Lee Il-Wha
    • Proceedings of the KSR Conference
    • /
    • 2005.11a
    • /
    • pp.836-841
    • /
    • 2005
  • As gravel roadbed is gradually turn down by repetitive loading of train, lasting work for maintenance and management of track is necessary to reduce the settlement of roadbed and the deterioration of track. Despite of above weak point, gravel roadbed has become a basic structure of roadbed at the present time. But request for new structure of roadbed is recently presented to settle an increased transportation capacity of track, and to solve a shortage of the labor to handle maintenance work of track. Therefore, we begin to research about maintenance-free roadbed like paved track from two years ago. In this study, a series of tests were performed to clarify the characteristics of strength and permeability of concrete specimen, which is the upper part of paved track injected by cement mortar, by type of geotextile and finally to give a basic data for application of geotextile to the lower part of paved track.

  • PDF

Peridynamic simulation of brittle-ice crushed by a vertical structure

  • Liu, Minghao;Wang, Qing;Lu, Wei
    • International Journal of Naval Architecture and Ocean Engineering
    • /
    • v.9 no.2
    • /
    • pp.209-218
    • /
    • 2017
  • Sea ice is the main factor affecting the safety of the Arctic engineering. However, traditional numerical methods derived from classical continuum mechanics have difficulties in resolving discontinuous problems like ice damage. In this paper, a non-local, meshfree numerical method called "peridynamics", which is based on integral form, was applied to simulate the interaction between level ice and a cylindrical, vertical, rigid structure at different velocities. Ice in the simulation was freshwater ice and simplified as elastic-brittle material with a linear elastic constitutive model and critical equivalent strain criterion for material failure in state-based peridynamics. The ice forces obtained from peridynamic simulation are in the same order as experimental data. Numerical visualization shows advantages of applying peridynamics on ice damage. To study the repetitive nature of ice force, damage zone lengths of crushing failure were computed and conclude that damage zone lengths are 0.15-0.2 times as ice thickness.

Structural Health Monitoring Using Wavelet Packet Transform (웨이블렛 팩킷변환을 이용한 구조물의 이상상태 모니터링)

  • Kim, Han-Sang;Yun, Chung-Bang
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
    • /
    • 2004.11a
    • /
    • pp.619-624
    • /
    • 2004
  • In this research, the structural health monitoring method using wavelet packet analysis and artificial neural network (ANN) is developed. Wavelet packet Transform (WPT) is applied to the response acceleration of a 3 element-cantilever beam which is subjected to impulse load and Gaussian random load to decompose the response signal, then the energy of each component is calculated. The first ten largest components in magnitude among the decomposed components are selected as input to an ANN to identify the damage location and severity. This method successfully predicted the amount of damage in the structure when the structure is subjected to impulse load. However, when the beam is subjected to Gaussian random load which can be considered as ambient vibration it did not yield satisfactory results. This method is applicable to structures such as machinery gears that are subjected to repetitive loads.

  • PDF

Fundamental Study on Heat Transfer Enhancement Effect of Microscale Surface Wrinkles (마이크로 표면주름 형상에 따른 열전달 촉진효과 기초연구)

  • Park, Hee-Jin;Park, Sang-Hu
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
    • /
    • v.26 no.9
    • /
    • pp.447-452
    • /
    • 2014
  • We evaluated heat transfer characteristics of microscale wrinkles using a CFD (computational fluid dynamics) analysis. In order to verify the heat transfer effect of wrinkles having various shapes, we introduce wrinkling processes to generate few different shapes of wrinkles such as macroscale ($200{\sim}400{\mu}m$ width), microscale ($10{\sim}30{\mu}m$ width), and hierarchical (microscale on macroscale wrinkle) wrinkles, using repetitive-dividing-volume (RDV) method for single-shape of wrinkles and connected method of UV-weakly polymerization with thermal curing for hierarchical structure of winkles. The analysis results of simplified CFD model showed that heat flux on heated plate was changed by the shape of wrinkles on the plate. The increase in heat flux of about 2.6 times was achieved in the case where hierarchical wrinkle structure was used.