• Molecules and Cells
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• 제37권5호
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• pp.372-382
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• 2014

In this study, the chloroplast (cp) genome sequences from three early diverged leptosporangiate ferns were completed and analyzed in order to understand the evolution of the genome of the fern lineages. The complete cp genome sequence of Osmunda cinnamomea (Osmundales) was 142,812 base pairs (bp). The cp genome structure was similar to that of eusporangiate ferns. The gene/intron losses that frequently occurred in the cp genome of leptosporangiate ferns were not found in the cp genome of O. cinnamomea. In addition, putative RNA editing sites in the cp genome were rare in O. cinnamomea, even though the sites were frequently predicted to be present in leptosporangiate ferns. The complete cp genome sequence of Diplopterygium glaucum (Gleicheniales) was 151,007 bp and has a 9.7 kb inversion between the trnL-CAA and trnV-GCA genes when compared to O. cinnamomea. Several repeated sequences were detected around the inversion break points. The complete cp genome sequence of Lygodium japonicum (Schizaeales) was 157,142 bp and a deletion of the rpoC1 intron was detected. This intron loss was shared by all of the studied species of the genus Lygodium. The GC contents and the effective numbers of codons (ENCs) in ferns varied significantly when compared to seed plants. The ENC values of the early diverged leptosporangiate ferns showed intermediate levels between eusporangiate and core leptosporangiate ferns. However, our phylogenetic tree based on all of the cp gene sequences clearly indicated that the cp genome similarity between O. cinnamomea (Osmundales) and eusporangiate ferns are symplesiomorphies, rather than synapomorphies. Therefore, our data is in agreement with the view that Osmundales is a distinct early diverged lineage in the leptosporangiate ferns.

• 생명과학회지
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• 제24권10호
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• pp.1046-1054
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• 2014

${\beta}$-1,3-glucanase는 다양한 바이오공정에 널리 사용되어지는 효소로서 산업적 이용가치 증대를 위해 ${\beta}$-1,3-glucanase의 대량생산이 요구되어 지고 있다. 본 연구에서는 반복서열 ${\delta}$-sequence에 의한 integration를 통해 Aspergillus oryzae유래의 ${\beta}$-1,3-glucanase (EXGA)의 과발현 유도를 연구하였다. 먼저 효모내의 여러 염색체상에 EXGA 유전자를 integration하기 위해 $pRS{\delta}K$-exgA와 $pRS{\delta}K$-exgA 플라스미드를 구축하였다. 이 플라스미드는 유전자의 구성적 발현을 위한 ADH1 프로모터, 분비생산을 위한 signal sequence와 ${\beta}$-1,3-glucanase유전자의 integration을 위한 ${\delta}$-sequence를 포함하고 있다. 먼저 $pRS{\delta}K$-exgA 플라스미드를 $BY4742{\Delta}exg1$ 균주에 형질전환하고, 재조합 ${\beta}$-1.3-glucanase가 안정하게 과발현 및 분비생산됨을 확인하였다. 다음으로 geneticin 선별을 통한 integration 유도와 ${\beta}$-1,3-glucanase 활성과의 관계를 조사해보기 위해 $pRS{\delta}K$-exgA 플라스미드를 $BY4742{\Delta}exg1$ (YKY082) 균주에 도입한 결과, geneticin 농도 증가에 따라 ${\beta}$-1,3-glucanase 활성도 증가되었고, geneticin 농도 0.8 mg/ml가 ${\beta}$-1,3-glucanase과발현에 적합한 농도임을 확인 할 수 있었다. 이어서 $pRS{\delta}K$-exgA 플라스미드는 연속적 ${\delta}$-integration에 의해 효모세포 내에 도입되어, 한번, 두번, 세번 그리고 네번의 integration에 의해 ${\beta}$-1,3-glucanase의 비활성은 0.063, 0.095, 0.131 그리고 0.165 unit/ml/$OD_{600}$로 증가되었다. 또한 연속적 integration에 의해 ${\beta}$-1,3-glucanase의 활성이 증가됨에 따라 다양한 염색체에 도입된 EXGA 유전자의 복제수(integration 빈도)도 같이 증가되었음을 확인하였다. 따라서 본 연구 결과는 반복적 ${\delta}$-sequence integration방법을 통해 재조합 ${\beta}$-1,3-glucanase의 활성을 점진적으로 안정하게 증가시킬 수 있음을 제시하였다.

• 제어로봇시스템학회논문지
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• 제11권9호
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• pp.802-809
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• 2005

A hybrid genetic algorithm is proposed for the problem of generating laser torch paths to cut a stock plate nested with free-formed parts each having a set of sub-parts. In the problem, the total unproductive travel distance of the torch is minimized. The problem is shown to be formulated as a special case of the standard travelling salesman problem. The hybrid genetic algorithm for solving the problem is hierarchically structured: First, it uses a genetic algorithm to find the cutting path f3r the parts and then, based on the obtained cutting path, sequence of sub-parts and their piercing locations are optimally determined by using a combined genetic and heuristic algorithms. This process is repeated until any progress in the total unproductive travel distance is not achieved. Computational results are provided to illustrate the validity of the proposed algorithm.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.592-595
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• 2000

In human chromosome, a short sequence of DNA has been repeated a number of times. These repeats are called variable number of tandem repeat(VNTR) or short tandem repeat(STR) which has short repeat core. VNTR and STR are used in the field of forensic science, evolution, and anthropology. In this work, we examined allele frequencies of 3 VNTR(YNZ22, NeuR, D21S11) and one STR(Humth01) in a Korean population sample by polymerase chain reaction(PCR) followed by high-resolution polyacrylamide gelelectrophoresis(PAGE) with silver staining. Subsequently, the polymorphism information content(PIC) was calculated : the highest PIC was observed for the NeuR locus(0.95680) and lowest for the Humth01 locus(0.75809).

• Journal of the Korean Statistical Society
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• 제35권4호
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• pp.355-376
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• 2006

To identify whether the sequence of observations follows a chain dependent process and whether the chain dependent or repeated observations follow stationary process or not, alternative procedures are suggested in this paper. These test procedures are formulated on the basis of logistic regression model under the likelihood ratio test criterion and applied to the daily rainfall occurrence data of Bangladesh for selected stations. These test procedures indicate that the daily rainfall occurrences follow a chain dependent process, and the different types of transition probabilities and overall transition probabilities of Markov chain for the occurrences of rainfall follow a stationary process in the Mymensingh and Rajshahi areas, and non-stationary process in the Chittagong, Faridpur and Satkhira areas.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2003년도 신호처리소사이어티 추계학술대회 논문집
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• pp.363-366
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• 2003

We propose the system that automatically adjusts the white balance on display products to a standard value according to several nations. We replace manual or semi-auto method with fully automatic method using windows application program. And we use RS-232C serial interface to communicate PC with display products which we want to adjust white balance. The PC generates patterns for measuring color information and Color Analyzer measures color and brightness. This value is transmitted through RS-232C serial interface to PC. The PC's algorithm analyzes this information and then decides which RGB Gain value is best for optimal white balance. This RGB Gain value is transmitted through RS-232C serial interface to display products. The modified color value is measured again and feed back to PC. This sequence is repeated until optimum white balance is obtained.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제1권2호
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• pp.157-166
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• 1997

The biosorption performances of copper were investigated by the immobilized biomass of nonliving marine brown algae Undaria pinnatifida by each of the Ca-alginate method(Ca-ALG), Ba-alginate method(Ba-ALG), polyethylene glycol method(PEG), and carrageenan method (CARR). The copper removal performance increased but the copper uptake decreased as the biomass amount was increased. However, the copper uptake by the immobilized biomass increased with increasing initial copper concentration. Among the immobilization methods, the copper uptake decreased in the following sequence: Ca-ALG > Ba-ALG > PEG > CARR. The pattern of copper uptake by the immobilized biomass fitted the Langmuir isotherm better than the Freundlich isotherm. Desorption of deposited copper with 0.05 ~0.5M HCI, resulted in no changes of the copper uptake capacity of the immobilized biomass by the immobilization methods except for PEG, through five subsequent biosorption/desorption cycles. There was no damage to the immobilized biomass which retained its macroscopic appearance in repeated copper uptake/elution cycles.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제5권3호
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• pp.542-559
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• 2011

Most game-theoretic works of Aloha have emphasized investigating Nash equilibria according to the system state represented by the number of network users and their decisions. In contrast, we focus on the possible change of nodes' utility state represented by delay constraint and decreasing utility over time. These foregone changes of nodes' state are more likely to instigate selfish behaviors in networking environments. For such environment, in this paper, we propose a repeated Bayesian slotted Aloha game model to analyze the selfish behavior of impatient users. We prove the existence of Nash equilibrium mathematically and empirically. The proposed model enables any type of transmission probability sequence to achieve Nash equilibrium without degrading its optimal throughput. Those Nash equilibria can be used as a solution concept to thwart the selfish behaviors of nodes and ensure the system stability.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.418-418
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• 2012

We fabricated a new type of hybrid film using molecular layer deposition (MLD). The MLD is a gas phase process analogous to atomic layer deposition (ALD) and also relies on a saturated surface reaction sequentially which results in the formation of a monolayer in each sequence. In the MLD process, polydiacetylene (PDA) layers were grown by repeated sequential surface reactions of titanium tetrachloride and 2,4-hexadiyne-1,6-diol with ultraviolet (UV) polymerization under a substrate temperature of $100^{\circ}C$. Ellipsometry analysis showed a self-limiting surface reaction process and linear growth of the hybrid films. Polymerization of the hybrid films was confirmed by infrared (IR) spectroscopy and UV-Vis spectroscopy. Composition of the films was confirmed by IR spectroscopy and X-ray photoelectron (XP) spectroscopy. The titanium oxide cross-linked polydiacetylene (TiOPDA) hybrid films exhibited good thermal and mechanical stabilities.

• 디지털콘텐츠학회 논문지
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• 제8권3호
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• pp.385-391
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• 2007

HFC 망 상향채널의 용량을 증대시키기 위한 차세대 케이블 모뎀 기술의 하나인 S-DMT 방식 케이블 모뎀의 주파수 동기 알고리즘 제안하고 성능을 평가하였다. 다수 반송파 전송 시스템의 주파수 동기 알고리즘들에 대한 분석을 통하여, 데이터 프레임의 프리앰블 필드 내에 약속된 반복 패턴의 훈련열을 삽입하여 전송하고, 파일롯 신호를 이용하여 잔류 주파수 오프셋에 따른 위상오차를 보정하는 방법을 제안하고 시뮬레이션을 통해 AWGN 잡음 환경에서의 성능을 평가 하였다. 성능 평가 결과, 파일롯 신호의 수와 크기에 따라 성능 차이를 보이나, 제안한 주파수 동기 알고리즘이 신속한 동기를 필요로 하는 S-DMT 방식 상향채널 케이블 모뎀에 적합하고 양호한 성능을 보임을 확인하였다.