• Title/Summary/Keyword: repeat unit

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Function and Oligomerization Study of the Leucine Zipper-like Domain in P13 from Leucania separata Multiple Nuclear Polyhedrosis Virus

  • Du, Enqi;Yao, Lunguang;Xu, Hua;Lu, Songya;Qi, Yipeng
    • BMB Reports
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    • v.40 no.2
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    • pp.232-238
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    • 2007
  • The p13 gene is uniquely present in Group II nucleopolyhedroviruses (NPVs) and some granuloviruses, but not in Group I NPVs. p13 gene was first described by our laboratory in Leucania separatamultiple nuclear polyhedrosis virus (Ls-p13) in 1995. However, the functions of Ls-P13 and of its homologues are unknown. When Ls-p13 was inserted into Autographa californica nucleopolyhedrovirus, a Group I NPV, polyhedra yield was inhibited. However, this inhibition was prevented when the leucine zipper-like domain of Ls-p13 was mutated. To determine the cause of this marked difference between Ls-P13 and leucine zipper mutated Ls-P13 (Ls-P13mL), oligomerization and secondary structure analyses were performed. High performance liquid chromatography and yeast two-hybrid assays indicated that neither Ls-P13 nor Ls-P13mL could form oligomers. Informatics and circular dichroism spectropolarimetry results further indicated marked secondary structural differences between Ls-P13 and Ls-P13mL. The LZLD of Ls-P13 has two extended heptad repeat units which form a hydrophobic surface, but it is short of a third hydrophobic heptad repeat unit for oligomerization. However, the mutated LZLD of Ls-P13mL lacks the above hydrophobic surface, and its secondary structure is markedly different. This difference in its secondary structure may explain why Ls-P13mL is unable to inhibit polyhedra yield.

Partial Retransmission Turbo HARQ Using the Sign Transitions of A Posteriori Values (사후 정보 값의 부호 변화를 이용한 부분 재전송 방식의 터보 HARQ)

  • Jang, Yeon-Soo;Yoon, Dong-Weon;Hyun, Kwang-Min;Lee, Sang-Hyun
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.22 no.8
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    • pp.768-775
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    • 2011
  • Since error compensation schemes guaranteeing high reliability are required in wireless communication systems for the transmission of the large amount of data, as an efficient error compensation scheme the turbo HARQ scheme combining automatic repeat request and turbo coding has been studied in many places in the literature. In the case of conventional turbo HARQ schemes, the transmitter recognizing NAK signals repetitively sends the whole unit packet to the receiver although the received packet can be partially correctable. Through two successive processes, selection of uncorrectable error data and retransmission of only the relevant parts of the information data, transmission efficiency can be improved. In this paper, we present an error data selection criterion for retransmission using the sign transitions of A Posteriori values and propose a tubo HARQ scheme based on the partial retransmission technique. Through a computer simulation, we show and analyze the performance of the proposed scheme with transmission efficiency.

Analysis of Viterbi Algorithm for Low-power Wireless Sensor Network (저전력 무선 센서네트워크를 위한 비터비 알고리즘의 적용 및 분석)

  • Park, Woo-Jun;Kim, Keon-Wook
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.44 no.6 s.360
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    • pp.1-8
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    • 2007
  • In wireless sensor network which uses limited battery, power consumption is very important factor for the survivality of the system. By using low-power communication to reduce power consumption, error rate is increased in typical conditions. This paper analyzes power consumption of specific error control coding (ECC) implementations. With identical link quality, ECC provides coding gain which save the power for transmission at the cost of computing power. In sensor node, transmit power is higher than computing power of Micro Controller Unit (MCU). In this paper, Viterbi algerian is applied to the low-transmit-power sensor networks in terms of network power consumption. Practically, Viterbi algorithm presents 20% of reduction of re-transmission in compared with Auto Repeat Request (ARQ) system. Furthermore, it is observed that network power consumption is decreased by almost 18%.

Effect of Types of Force-fields on Gas Transport Thorough Polymer Membrane (Force-field가 고분자 분리막의 기체 투과거동에 미치는 영향)

  • Lee, Ji-Su;Park, Chi Hoon
    • Membrane Journal
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    • v.29 no.1
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    • pp.37-43
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    • 2019
  • In this study, we investigated how the force-field, which is the most important factor to define atomic motion in molecular dynamics (MD), affects the motion of the polymer and gas molecules. The repeat units and the polymer structure were well simulated in all five force-fields, and the distribution of the polymer linear chain in the final polymer 3D model did not show any significant difference. However, the movement of actual gas molecules showed a very different tendency, which was also observed in COMPASS and pcff using the same functional form. Therefore, even if the same structure is used, it can be seen that the motion of the gas molecule moves under the influence of the force-field continuously over time, so that the effect is much larger than that of macromolecules such as a polymer linear chain. Accordingly, in case of using different force-fields, it is necessary to be very careful in comparison of those results.

Object Classification Method Using Dynamic Random Forests and Genetic Optimization

  • Kim, Jae Hyup;Kim, Hun Ki;Jang, Kyung Hyun;Lee, Jong Min;Moon, Young Shik
    • Journal of the Korea Society of Computer and Information
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    • v.21 no.5
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    • pp.79-89
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    • 2016
  • In this paper, we proposed the object classification method using genetic and dynamic random forest consisting of optimal combination of unit tree. The random forest can ensure good generalization performance in combination of large amount of trees by assigning the randomization to the training samples and feature selection, etc. allocated to the decision tree as an ensemble classification model which combines with the unit decision tree based on the bagging. However, the random forest is composed of unit trees randomly, so it can show the excellent classification performance only when the sufficient amounts of trees are combined. There is no quantitative measurement method for the number of trees, and there is no choice but to repeat random tree structure continuously. The proposed algorithm is composed of random forest with a combination of optimal tree while maintaining the generalization performance of random forest. To achieve this, the problem of improving the classification performance was assigned to the optimization problem which found the optimal tree combination. For this end, the genetic algorithm methodology was applied. As a result of experiment, we had found out that the proposed algorithm could improve about 3~5% of classification performance in specific cases like common database and self infrared database compare with the existing random forest. In addition, we had shown that the optimal tree combination was decided at 55~60% level from the maximum trees.

Implementation of autonomous driving algorithm and monitoring application for terrain navigation (지형 탐색 자율주행 알고리즘과 모니터링 애플리케이션 구현)

  • Kang, Jongwon;Jeon, Il-Soo;Kim, Myung-Sik;Lim, Wansu
    • Journal of IKEEE
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    • v.25 no.3
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    • pp.437-444
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    • 2021
  • In this paper, we propose an autonomous driving algorithm that allows a robot to explore various terrains, and implement an application that can monitor the robot's movement path during terrain search. The implemented application consists of a status unit that indicates the position, direction, speed, and motion of the mobile robot, a map unit that displays terrain information obtained through terrain search, and a control unit that controls the movement of the mobile robot. In order to control the movement of the robot, only the start and stop of the search/return is commanded by the application, and all driving for the search is performed autonomously. The basic algorithm for terrain search uses an infrared sensor to check for obstacles in the order of left, front, right, and rear, and if there is no obstacle and the path traveled is a dead end, it returns to the previous position and moves in the other direction to continue the search. Repeat the process to explore the terrain.

Analysis of Microsatellite Patterns in the Genome of Red Sea Cucumber (홍해삼 유전체 분석에 의한 microsatellite의 분포도 연구)

  • Lee, Tae Wook;Kim, Sam Woong;Kim, Jung Sun;Chi, Won-Jae;Bang, Woo Young;Kim, Jang Hyeon;Yang, Chul Woong;Bang, Kyu Ho;Gal, Sang Wan
    • Journal of Life Science
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    • v.32 no.9
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    • pp.690-697
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    • 2022
  • This study was conducted to analyze genome of red sea cucumber and to use it as basic data for the development of genetic markers for red sea cucumber. Microsatellite marker analysis of Ulleungdo_normal and Ulleungdo_native red sea cucumbers revealed that dinucleotide simple sequence repeats (SSRs) had the highest ratio, at 81.3~81.4%, and the number of the detected SSRs tended to decrease as the number of repeating sequence units in SSRs increased. In general, microsatellites with between 5 and 10 iterations were most common. As the size of the SSR repeating sequence units increased, the SSR iterations gradually decreased. The di-, tri-, and tetra-nucleotides in SSRs were detected in the highest numbers as (AT)5, (AAT)5, and (AAAT)5, respectively. (CG) and (CCG) had very low frequencies compared to the numbers of other repeating SSR units. The numbers of di-and tri-nucleotide repeats were up to 35 and 32, respectively, and then increased discontinuously up to 44 and 43 repeats, respectively. Tetra-, penta-, and hexa-nucleotides in SSRs occurred in numbers up to 25, 21 and 14, respectively. This analysis of red sea cucumber indicated that it maintains its own repetition sequence and repetition number; therefore, we suggest that using it as basic data for molecular marker will be possible in future research.

Neovascularization in Outer Membrane of Chronic Subdural Hematoma : A Rationale for Middle Meningeal Artery Embolization

  • Hyun Kim;Yoori Choi;Youngsun Lee;Jae-Kyung Won;Sung Ho Lee;Minseok Suh;Dong Soo Lee;Hyun-Seung Kang;Won-Sang Cho;Gi Jeong Cheon
    • Journal of Korean Neurosurgical Society
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    • v.67 no.2
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    • pp.146-157
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    • 2024
  • Objective : Chronic subdural hematomas (cSDHs) are generally known to result from traumatic tears of bridging veins. However, the causes of repeat spontaneous cSDHs are still unclear. We investigated the changes in vasculature in the human dura mater and outer membrane (OM) of cSDHs to elucidate the cause of their spontaneous repetition. Methods : The dura mater was obtained from a normal control participant and a patient with repeat spontaneous cSDHs. The pathological samples from the patient included the dura mater and OM tightly adhered to the inner dura. The samples were analyzed with a particular focus on blood and lymphatic vessels by immunohistochemistry, 3-dimensional imaging using a transparent tissue clearing technique, and electron microscopy. Results : The dural border cell (DBC) layer of the dura mater and OM were histologically indistinguishable. There were 5.9 times more blood vessels per unit volume of tissue in the DBC layer and OM in the patient than in the normal control. The DBC layer and OM contained pathological sinusoidal capillaries not observed in the normal tissue; these capillaries were connected to the middle meningeal arteries via penetrating arteries. In addition, marked lymphangiogenesis in the periosteal and meningeal layers was observed in the patient with cSDHs. Conclusion : Neovascularization in the OM seemed to originate from the DBC layer; this is a potential cause of repeat spontaneous cSDHs. Embolization of the meningeal arteries to interrupt the blood supply to pathological capillaries via penetrating arteries may be an effective treatment option.

Synthesis and Properties of Poly(ester-imide) Resin for High Temperature Resistant Electrical Insulation (고내열성 전기 절연용 Poly(ester-imide) 수지의 합성 및 물성)

  • Huh, Wansoo;Lee, SangWon;Kim, Jeongyeol;Park, Leesoon;Kim, Soonhak;Haw, JungRim
    • Applied Chemistry for Engineering
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    • v.10 no.5
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    • pp.767-771
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    • 1999
  • Poly(ester-imide)(PEI) for the electrical insulation coating was synthesized and evaluated with one-step method as well as two-step method. For the synthesis of poly(ester-imide), imide repeat unit of N,N'-(4,4'-diphenylmethane) bistrimellitimide(DID) was initially made from trimellitic anhydride(TMA) and methylene dianiline(MDA), followed by the second stage reaction of esterification. One-step reaction was performed by reaction of TMA, MDA, dimethyl terephthalate(DMT), ethylene glycol(EG), and 1,3,5-tris-(2-hydroxy ethyl) isocyanurate(THEIC) in m-cresol solvent at a time. The synthesized poly(ester-imide) was cured with xylene, P-5030K(phenol-formaldehyde resin), TK-8(TDI type blocked polyisocyanate) and tetrapropyltitanate(TPT). It was found that the content of hydroxyl group, amount of DMT, and imide repeat unit played important role for the properties of electrical insulation coating film.

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Development of an ISSR-Derived SCAR Marker in Korean Ginseng Cultivars (Panax ginseng C. A. Meyer)

  • Lee, Jei-Wan;Kim, Young-Chang;Jo, Ick-Hyun;Seo, A-Yeon;Lee, Jeong-Hoon;Kim, Ok-Tae;Hyun, Dong-Yun;Cha, Seon-Woo;Bang, Kyong-Hwan;Cho, Joon-Hyeong
    • Journal of Ginseng Research
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    • v.35 no.1
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    • pp.52-59
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    • 2011
  • Recently, new ginseng cultivars having superior agricultural traits have been developed in Korea. For newly developed plant cultivars, the identification of distinctiveness is very important factors not only in plant cultivar management but also in breeding programs. Thus, eighty-five inter simple sequence repeat (ISSR) primers were applied to detect polymorphisms among six major Korean ginseng cultivars and two foreign ginsengs. A total of 197 polymorphic bands with an average 5.8 polymorphic bands and 2.9 banding patterns per assay unit across six Korean ginseng cultivars and foreign ginsengs from 236 amplified ISSR loci with an average 6.9 loci per assay unit were generated by 34 out of 85 ISSR primers. Three species of Panax ginseng including the Korean ginseng cultivars, P. quinquefolius, and P. notoginseng, could be readily discriminated using most tested primers. UBC-821, UBC-868, and UBC-878 generated polymorphic bands among the six Korean ginseng cultivars, and could distinguish them from foreign ginsengs. Sequence characterized amplified region (SCAR) marker system was introduced in order to increase the reproducibility of the polymorphism. One SCAR marker, PgI821C650, was successfully converted from the randomly amplified polymorphism by UBC-821. It showed the expected dominant polymorphism among ginseng samples. In addition, the specific polymorphism for Sunwon was generated by treating Taq I restriction enzyme to polymerase chain reaction products of PgI821C650. These results will serve as useful DNA markers for identification of Korean ginseng, especially Sunwon cultivar, seed management, and molecular breeding program supplemented with marker-assisted selection.