• Molecules and Cells
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• 제40권2호
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• pp.117-122
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• 2017

Despite the fact that porcine embryonic stem cells (ESCs) are a practical study tool, in vitro long-term maintenance of these cells is difficult in a two-dimensional (2D) microenvironment using cellular niche or extracellular matrix proteins. However, a three-dimensional (3D) microenvironment, similar to that enclosing the inner cell mass of the blastocyst, may improve in vitro maintenance of self-renewal. Accordingly, as a first step toward constructing a 3D microenvironment optimized to maintain porcine ESC self-renewal, we investigated different culture dimensions for porcine ICM-derived cells to enhance the maintenance of self-renewal. Porcine ICM-derived cells were cultured in agarose-based 3D hydrogel with self-renewal-friendly mechanics and in 2D culture plates with or without feeder cells. Subsequently, the effects of the 3D microenvironment on maintenance of self-renewal were identified by analyzing colony formation and morphology, alkaline phosphatase (AP) activity, and transcriptional and translational regulation of self-renewal-related genes. The 3D microenvironment using a 1.5% (w/v) agarose-based 3D hydrogel resulted in significantly more colonies with stereoscopic morphology, significantly improved AP activity, and increased protein expression of self-renewal-related genes compared to those in the 2D microenvironment. These results demonstrate that self-renewal of porcine ICM-derived cells can be maintained more effectively in a 3D microenvironment than in a 2D microenvironment. These results will help develop novel culture systems for ICM-derived cells derived from diverse species, which will contribute to stimulating basic and applicable studies related to ESCs.

• International Journal of Reliability and Applications
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• 제4권3호
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• pp.113-129
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• 2003

Based on moments inequalities new testing procedures are derived for testing exponentiality against new renewal better than used (NRBU) and renewal new better than used (RNBU). These classes play an important role in formulating repair or replacement policies. The asymptotic Pitman efficiency of (NRBU) and (RNBU) testes are studied. Selected critical values are tabulated for sample sizes n=5(1) 50. The power estimates for some commonly used life distributions in reliability are also calculated. Some set of real data is used as an example to elucidate the use of the proposed test statistic for practical reliability analysis. The problem in case of right-censored data is also handled.

• KIEAE Journal
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• 제12권3호
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• pp.61-67
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• 2012

The purpose of this study was to analyze the characteristics of the pre-design of brownfield's by comparing LDA, EPA, and BRA of UK and USA in terms of establish mainbody, brownfield information, development process, opening development information. Such analysis will generate important implications for the development and renewal of brownfield in Korea by gaining insights into the characteristics of brownfield that reflect systematic and effective management and planning for urban renewal. The findings from this study indicate that: 1. Information on brownfield plays an important role at the development and planning stages that precede architectural design. 2. There is a great need for organizations that are responsible for brownfield renewal, such as LDA of UK, EPA and BRA of USA, in Korea, to make information public and to practice systematic management. 3. Discussion on the pre-design that follows contamination and renewal of brownfield and the developmental strategies that satisfy both the developmental and public profits are warranted.

• 한국BIM학회 논문집
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• 제12권1호
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• pp.35-41
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• 2022

This research aims to find the issues and explore policy agenda of contributed acceptance of urban renewal project. Through the analysis of urban renewal project in Seoul, three isssues was found as follows. Firstly, the ratio of contributed acceptance in each district was quite different. Secondly, the donated land was scattered around whole project area. Thirdly, infrastructures are not constructed until all renewal projects were implemented. For this issues, this research proposed that a proper standard of the ratio of contributed acceptance is needed and land donation should be designated to adjacent lot or block. Also public support should be for developing urban capacity and completing infrastructure.

• 국제학술발표논문집
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• The 1th International Conference on Construction Engineering and Project Management
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• pp.649-654
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• 2005

A new law, the Urban and Living Environment Improvement Act, was established, promulgated, and entered into by the Ministry of Construction and Transportation as of 1st July 2003. This system under this law, which improves currently executed consulting process and upgrade its level, is to execute the legal process and procedures that are necessarily required for housing reconstruction/renewal project.. This system requires to be supplemented with addition of reasonable, logical, and systemized tools of system. Through concurrent application of professional management of renewal business(PMRB), more benefit will be provided to housing renewal associations that are constituted with residences concerned for execution of CM project.

• 한국습지학회지
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• 제9권3호
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• pp.63-74
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• 2007

In this Century, most of ports have been changing in order to keep up with change of the world. The ports are became larger and integrated with high technology because containers which pass through the ports are sharply increasing. Not to turn over the world wide competition of the ports, a renewal of Bukhang in Busan was promoted firstly in Korea. In this study, final draft renewal plan of Bukhang in Busan at this stage was compared with renewal plan of Haffencity in Germany in order to propose improved renewal plan of Bukhang in Busan. Renewal plan of Haffencity in Germany was very similar with Bukhang in Busan in terms of renewal period and direction. From the results of these comparisons, it was concluded that participation of private enterprise and international capital should be invested. Also, the facility plan of Bukhang was suggested to vitalize citizen's participation and embodimentof renewal plan. Prior to making a development plan enough consideration on the preservation of the environment is needed for the environmentally sound and sustainable development (ESSD). Also Korean tradition and culture should have to be considered in order to create the most Korean appearance.

• 스마트미디어저널
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• 제12권5호
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• pp.111-121
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• 2023

본 연구의 목적은 브랜드 관리 측면에서 로고가 브랜드 아이덴티티를 대변하는 중요한 시각적 도구로 보고 브랜드 수명주기에 따른 로고 리뉴얼 디자인 방법을 모색하는 것이다. 본 연구는 브랜드 수명주기와 브랜드 리뉴얼 전략에 대한 문헌연구와 로고 리뉴얼 디자인에 관한 전문가 설문, 브랜드의 수명주기를 판별 할 수 있는 통계자료가 있는 35개 식음료 브랜드의 로고 분석으로 진행되었다. 연구 결과는 크게 세 가지이다. 첫째, 네 단계(도입기, 성장기, 성숙기, 쇠퇴기)의 브랜드 수명주기의 특징과 리뉴얼 전략을 도출했다. 둘째, 네 가지 브랜드 리뉴얼 전략(부분변경, 전체변경, 리포지셔닝, 새 이미지 창출)과 수명주기를 기준으로 로고 리뉴얼 디자인 방향을 제안하였다. 이를 토대로 수명주기별 로고 리뉴얼 특징과 관계성을 제안하였다. 셋째, 브랜드 수명주기와 브랜드 리뉴얼 전략에 따라 중요하게 적용되는 아이덴티티 시각요소(그래픽 요소의 첨삭, 톤/매너, 서체, 시그니처 컬러 변경)를 발견했다. 성장기와 성숙기의 부분변경전략에 그래픽 요소의 첨삭과 톤 변경이 중요하며, 리포지셔닝 전략과 새이미지 창출에 시그니처 컬러 변경이 중요함을 발견했다.

• 대한수학회보
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• 제48권2호
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• pp.325-334
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• 2011

In this paper we consider the discrete time delayed renewal risk model. We investigate what will happen when the distribution function of the discounted proper deficit is asymptotic in the initial surplus. In doing this we establish several lemmas regarding some related ruin quantities in the discrete time delayed renewal risk model, which are of significance on their own right.

• Journal of the Korean Statistical Society
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• 제33권3호
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• pp.299-302
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• 2004

A Markovian approach is introduced to find the Laplace transform of the forward recurrence time in the renewal process at finite time t > 0. Until now, most works on the forward recurrence time have been done through renewal arguments.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2003년도 춘계학술발표회 논문집
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• pp.479-484
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• 2003

The renewal of digital map takes much time and the manual process. LiDAR data allows reduction of time, automatic manner, and acquisition of the precise position. So it is used to renew the digital map of 1:5,000 scale. From the accuracy test results using aerial imagery and digitizing, renewal of digital map are feasible in automatic manner to some extent.