• Journal of Microbiology and Biotechnology
• /
• v.25 no.6
• /
• pp.782-787
• /
• 2015

In this study, we developed an assay system for missense mutations in human phenylalanine hydroxylases (hPAHs). To demonstrate the reliability of the system, eight mutant proteins (F39L, K42I, L48S, I65T, R252Q, L255V, S349L, and R408W) were expressed in a mutant strain (pah^-) of Dictyostelium discoideum Ax2 disrupted in the indigenous gene encoding PAH. The transformed pah - cells grown in FM minimal medium were measured for growth rate and PAH activity to reveal a positive correlation between them. The protein level of hPAH was also determined by western blotting to show the impact of each mutation on protein stability and catalytic activity. The result was highly compatible with the previous ones obtained from other expression systems, suggesting that Dictyostelium is a dependable alternative to other expression systems. Furthermore, we found that both the protein level and activity of S349L and R408W, which were impaired severely in protein stability, were rescued in HL5 nutrient medium. Although the responsible component(s) remains unidentified, this unexpected finding showed an important advantage of our expression system for studying unstable proteins. As an economic and stable cell-based expression system, our development will contribute to mass-screening of pharmacological chaperones for missense PAH mutations as well as to the in-depth characterization of individual mutations.

• The Journal of Industrial Distribution & Business
• /
• v.8 no.6
• /
• pp.75-86
• /
• 2017

Purpose - The purpose of this study is to grasp the concept, characteristics and application status of sharing economy, and to derive a research model based on sharing economic service, and to analyze factors and influences of consumers' intention to reuse of sharing economy. Research design, data, and methodology - The questionnaires were created to examine variables for practical and theoretical implications. After pilot survey, conducted for 24 days from March 10th to April 2st in 2017, total numbers were 377. But 330 copies were used for the analysis with IBM SPSS Statistics 23.0 and IBM SPSS AMOS 23.0. The structural equation model was applied for this. Results - First, sharing economic services remain at an early stage, but it is meaningful to identify the revenue mechanism of the business model of the sharing economic platform. Second, in this study, it is meaningful that we systematized the theoretical structure by examining existing studies on the characteristics of the sharing economic service and consumer characteristics, and by examining empirically. Third, Satisfaction and Reliability are related to the characteristics of Sharing Economic Service (Security, Convenience, Discount, Sharing, Social Interaction), Consumer Characteristics (Personal Innovation, Word-of-Mouth) It is meaningful to broaden the understanding of the factors by verifying the mediating effect. Fourth, the sharing economy business is meaningful in that it is a new consumption trend that changes the meaning of consumption to consumers. Gradually, more and more people are recalling that purchasing something is not consumption, but sharing and borrowing is also consumption. In other words, through the sharing economy, consumers can experience more products and services, have more choices, and are expected to have a positive impact on economic growth by increasing the utilization of idle resources. Conclusions - Currently, the sharing economy is growing rapidly all over the world. Therefore, in the subsequent study, it is necessary to compare Korea and China's sharing economy and study the cultural and social characteristics of Korea and China. In particular, I think that steady research is necessary for more precise and specific direction on the influence of the shared economy.

• Journal of Navigation and Port Research
• /
• v.36 no.10
• /
• pp.889-893
• /
• 2012

The growth of Chinese logistics market has brought many logistics enterprises enter into the chinese market. The purpose of this study is to suggest some alternatives for activating enter into chinese market for smaller-sized korean logistics enterprise. First of all, in order to achieve such a purpose, factors for activation through literature survey and previous studies are analyzed. After that, questionnaire survey are carried out. Also factor analysis and reliability test based on the questionnaire results are performed. Main findings are suggested as followings. First, in order to activate entering the chinese market, it is drawn out that possessing of logistics infrastructure and understanding of culture of chinese relationship are most important. Second, market information and reform of management system, inspection and customs clearance are suggested as less important.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.27 no.1
• /
• pp.1-7
• /
• 2007

As fiber reinforced composite materials are widely used in aircraft, space structures and robot arms, the study on non-destructive testing methods has become an important research area for improving their reliability and safety. AE (acoustic emission) can evaluate the defects by detecting the emitting strain energy when elastic waves are generated by the initiation and growth of crack, plastic deformation, fiber breakage, matrix cleavage, or delamination. In the paper, AE signals generated under uniaxial tension were measured and analyzed using the $8{\times}8$ matrix piezo electric sensor. The electronic circuit to control the transmitting distance of AE signals was designed and constructed. The optical data storage system was also designed to store the AE signal of 64channels using LED (light emitting diode) elements. From the tests, it was shown that the source location and propagation path of AE signals in composite materials could be detected effectively by the $8{\times}8$ matrix piezo electric sensor.

• The Research Journal of the Costume Culture
• /
• v.26 no.5
• /
• pp.649-664
• /
• 2018

With the rapid growth of the Chinese cosmetics market and the continuous introduction of new products into the market, beauty videos generated by other consumers, that are by beauty vloggers, have become a major source of information for cosmetics consumers. With the popularity of beauty vlogs in China, beauty vloggers have become influencers who affect consumers' information processing and attitude development regarding cosmetic products and beauty routines. The current study aims to explore the characteristics of beauty vloggers that affect consumers' attitudes toward both the beauty vlogs and the products featured in those vlogs. Based on parasocial interaction theory, the study examines whether perceived characteristics of beauty vloggers influence consumers' perceived parasocial interaction, which in turn influences their attitudes. The study employs an online survey, which was administered to female consumers in China. Data from a total of 372 responses was used for analysis. Findings show that the perceived similarities to and perceived trustworthiness of vloggers have a significant impact on parasocial interactions, which influences consumer attitudes toward both the vlogs and the products shown in them. Perceived reliability and perceived attractiveness are shown to have a positive impact on consumer attitudes toward vlogs. Perceived expertise, attractiveness, similarity, and trustworthiness have all been shown to affect product attitudes. These results show that while beauty vlogs have a significant impact on consumers' decision-making processes, the perceived characteristics of beauty vloggers themselves are important to consumers in the development of their attitudes toward both vlogs and products.

• Journal of Digital Convergence
• /
• v.11 no.11
• /
• pp.265-278
• /
• 2013

This study is about how does collaboration activities of large company's Supplier Relationship(SR) effect on manufacturing capability and performance of a SMEs. In this research, there are many ways to analyze data. The methods used in the study are hypothesis testing and empirical testing based on SPSS 21.0 and AMOS21.0, factorial analysis for validity and reliability of questionnaire item, construct validity and variance extracted as a research model, and subcontract-tiers as a moderating variable. As a result, the effect of the collaboration activities of large business did not have the significant effect on supplier's manufacturing capability. It showed different result by subcontract-tiers In first-tier cooperative, collaboration activities of large business had significant effect on manufacturing capability, but it did not have the significant effect on performance. In second/third-tier cooperative, manufacturing capability and performance are significantly affected by manufacturing practices. In conclusion, this study will suggest the direction how to achieve an accompanied growth between large enterprises and SMEs.

• Journal of Information Technology Services
• /
• v.14 no.3
• /
• pp.99-116
• /
• 2015

The concept of Cloud computing has been introduced in the IT field over 10 years and industry has been expanding constantly. However, compare to the maturity of global market, Korea cloud computing industry is only in the early stage. Even the Korea has advantages in technology infrastructure; the pace of Korea cloud computing market growth is taking a serious downturn. Under these circumstances, it is needed to be discussing that strategy for expanding the cloud computing market size and for sustaining global competitiveness of local companies. Previous studies on plans for Korea cloud computing market has been conducted since 2009 and most of them are tend to examined in demand perspective. Thus, this study aims at identifying the priority of business challenges for making better performance in the market with service provider aspects. To analyze the important factors in the providing cloud computing service, ANP methodology was applied in this study. The network model including five clusters, security, stability, performance, consumer, and institution, was defined through literature review and expert survey was conducted to collect data. As a result of ANP analysis, 'Securing service reliability' was analyzed as the most important factor and followed by 'Preparing the range of legal liability', 'Preventing personal information leakage' and 'Preventing confidential information data leakage.' The priority of result indicates that service provider needs to focus on to make the secured service environment. This study has significance on analyzing the priority of business challenges in the service provider perspective. This study will provide useful guidelines to for establishing strategies in cloud computing market.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
• /
• 1997.11a
• /
• pp.392-403
• /
• 1997

There is a strong international move towards performance based fire regulations for buildings with New Zealand and Australia at the forefront of research in this fold. The reform of regulations is thought to offer more innovation and flexibility in building design and greater cost effectiveness in construction. An important part of the research in this area is related to the development of agreed approaches to fire safety design, such as the Fire Code Reform Centre's "Fire Engineering Guidelines" or New Zealand's "Fire Engineering Design Guide". Such design process documents have incorporated or referenced much of the latest research in areas such as: tenability criteria fire compartment models egress models risk assessment. Use of such design guidelines or equivalents in major projects in countries such as Hong Kong and Australia have highlighted where fro engineering can offer real benefits to building designers and ultimately building owners and operators. However, there is still much research to be done and use of a systematic, logical design approach clearly identifies where design data or modelling techniques are still urgently required. Such areas are: fire growth rates and peak heat release rates for non-residential occupancies pre-movement times related to egress experimental validation and limits of applicability of CFD and other compartment Ire models probability/reliability data on fire protection systems for risk based analysis. Examples from case studies will be shown where lack of such research and poor judgement can lead to inferior design solutions or where unnecessarily conservative designs can lead to cost excesses. In summary, the link between Ire engineering designers and the research community is very important to highlight areas of fire research that will have the most benefit to the building and construction industry.nstruction industry.

• Journal of Aerospace System Engineering
• /
• v.15 no.5
• /
• pp.50-59
• /
• 2021

The need for risk based safety management has been highlighted in accordance with the growth of aviation industry and public interest in aviation safety. Maintenance, Repair and Overhaul (MRO) in aviation industry is defined to guarantee the safety and reliability. Therefore, state's Civil Airworthiness Authority (CAA) has established safety oversight system dedicated for the development of a healthy MRO industry. The study proposed a risk based oversight system considering Korean environment. Based on literature review including analysis of International Civil Aviation Organization guidance and advance system, we identified two organization risks. Organization complexity can be evaluated in accordance with how large the organization is and how complicated the service they provide are. Organization profile is the organization's characteristic related with safety. This study contributes to improving the process of identifying risk of organization and focusing surveillance capabilities on risk areas for efficient management of CAA's resources.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.397-402
• /
• 2009

Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.