• KSBB Journal
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• 제24권3호
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• pp.259-266
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• 2009

AfsR2 과발현 S. lividans TK21을 이용하여 DNA microarray를 수행하였다. 그 결과, phosphate starvation과 관련 있는 42개의 유전자들이 up-regulated 되었으며, 특히 SCO4139 (pstB, phosphate ABC transport system ATP-binding protein)와 SCO4142 (pstS, phosphate-binding protein precursor)는 afsR2가 phosphate와 같은 nutrient starvation에 적극적으로 관여한다는 것을 나타내며, SCO4228 (putative phosphate transport system regulatory protein)은 기존에 수행되었던 2D-electrophoresis 연구나 afsS null S. coelicolor를 이용한 DNA microarray 연구에서도 공통적으로 보고되었던 유전자로서 phosphate lilitation에 대한 afsR2의 효과가 지속적으로 검증되고 있음을 뜻한다. 또한 afsR2 과발현을 통해서 sigma factor인 SCO2954 (sigL)과 SCO5147 (sigE)의 발현이 유도되었으며 두 유전자의 구조적인 특징을 고려해 보았을 때 afsR2가 RNA polymerase와의 linker로서의 역할을 추측해 볼 수 있다. 뿐만 아니라 whi 관련 유전자들의 발현 또한 afsR2에 의해 증가되었다. 이는 afsR2가 단순히 2차 대사물질 생합성 조절에만 관여하는 것이 아니라 형태적 분화에 작용함으로써 최종적으로 여러 2차 대사물질의 합성을 유도한다고 말할 수 있다. 이러한 결과들을 토대로 afsR2가 기존에 항생제 생합성에만 관여하는 global regulatory 조절인자가 아닌 방선균이 stationary phase로 전환되는 시점에서 형태적 분화에 영향을 미치고 phosphate limitation stress를 줄여주는 2차 대사의 key-factor regulatory 유전자임을 알 수 있다.

• 한국지구과학회지
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• 제37권3호
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• pp.173-185
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• 2016

이 연구의 목적은 고등학생의 시스템 사고가 과학 자기 효능감을 구성하는 요인인 자기 조절 효능감과 과학에 대한 자신감에 어떤 영향을 미치는가를 분석하는 것이다. 고등학생의 과학 자기 효능감에 영향을 미치는 요인으로 시스템 사고를 설정하고, 시스템 사고가 자기 조절 효능감을 매개로 과학에 대한 자신감에 영향을 미친다는 가설을 설정하였다. 광역시 소재 일반계 고등학교 2학년 중 이공계열로 진학하고자 하는 학생 210명을 대상으로 시스템 사고 검사 도구 20문항과 과학 자기 효능감 검사 도구 중에서 자기 조절 효능감을 알아보는 11문항과 자신감을 알아보는 8문항, 총 39문항을 투입하였으며 성실하지 못한 응답지 22부를 제외한 188부를 분석하였다. 탐색적 요인 분석 결과 시스템 사고 검사 도구는 20문항이 모두 의도한 요인에 적합한 결과가 나왔으며, 자기 조절 효능감은 8문항이, 자신감은 4문항이 적합한 결과가 나왔다. 구조 방정식 모델을 통한 모델 적합도를 살펴본 결과 시스템 사고 5개 요인 중 학생 개인의 사고과정을 살펴보는 시스템 분석, 개인숙련, 정신모델 3개 요인이 자기 조절 효능감 및 자신감에 영향을 주는 모델의 적합도가 가장 높은 적합도를 보여주었다(${\chi}^2$=344.498, df=242, TLI= .921, RMSEA= .044). 또한 시스템 분석, 개인숙련, 정신모델 중 시스템 분석과 정신모델이 직 간접적으로 과학에 대한 자신감에 영향을 미치고 있었으며, 정신모델의 경우 자기 조절 효능감을 매개로 하여 자신감에 영향을 미친다는 결과를 도출할 수 있었다. 연구에서 도출된 결과를 토대로 시스템 사고가 과학 학습의 정의적 영역에 미치는 영향을 알 수 있었으며, 또한 시스템 사고를 활용한 과학 학습에 대하여 구체적인 이론적 근거를 제시할 수 있을 것이다.

• 패션비즈니스
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• 제19권6호
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• pp.1-13
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• 2015

This study aimed to determine the relationship between consumer regulatory focus and impulse buying of apparel products and the mediating effect of consumer fashion innovativeness. A large portion of consumer buying behavior is based on impulse. However, impulse buying is difficult to predict; moreover, factors that explain consumers' impulse buying tendencies are not yet fully identified. In this study, consumer regulatory focus was considered as a factor that explains consumers' impulse buying tendencies. The purpose of this study was to examine how fashion innovativeness and impulse buying tendencies differ, based on the consumer regulatory focus. A structural equation modeling was conducted to validate the research model. A questionnaire agency conducted online survey for 1 week and of the 400 copies distributed, total of 400 were collected. Promotion-focus and fashion innovativeness were positively correlated, which in turn had an effect on rational, emotional, and situational impulse buying. The promotion-focus had a negative effect on fashion innovation, but a positive effect on emotional impulse buying. On the other hand, prevention-focus had negative effect on emotional impulse buying, and situational impulse buying. Consumer regulatory focus and fashion innovativeness were useful predictors different consumer tendencies of impulse buying of apparel.

• 한국미생물·생명공학회지
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• 제32권2호
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• pp.190-194
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• 2004

The gene product of dctD (DctD) activates transcription from the dctA promoter regulatory region by the $\sigma^{54}$ -holoenzyme form ofRNA polymerase ($E\sigma^{54}$ ) in Rhizobium meliloti and R. leguminosarum. The Escherichia coli integration host factor (IHF) stimulated DctD-mediated activation from the dctA promoter regulatory region of R. leguminosarum but not R. meliloti. In the absence of UAS, IHF inhibited DctD-mediated activation from both of these promoter regulatory regions. IHF also inhibited activation from R. leguminosarum dctA by nitrogen regulatory protein C (NtrC), another activator of $E\sigma^{54}$ but not by one which lacks a specific binding site in this promoter regulatory region. IHF, however, stimulated NtrC-mediated activation from the R. meliloti dctA promoter. Upon removal of the UAS, IHF inhibited NtrC-mediated transcription activation from the R. meliloti dctA promoter regulatory region. These data suggest that IHF likely faciliates productive contacts between the activators NtrC or DctD and $E\sigma^{54}$ to stimulate activation from dctA promoter.

• IMMUNE NETWORK
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• 제10권6호
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• pp.247-249
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• 2010

Foxp3 is a transcript factor for regulatory T cell development. Interestingly, Foxp3-expressing cells were identified in B cells, especially in CD19(+)CD5(+) B cells, while those were not examined in CD19(+)CD5(-) B cells. Foxp3-expressing CD5(+) B cells in this study were identified in human PBMCs and were found to consist of $8.5{\pm}3.5%$ of CD19(+)CD5(+) B cells. CD19(+)CD5(+)Foxp3(+) B cells showed spontaneous apoptosis. Rare CD19(+)CD5(+) Foxp3(+) regulatory B cell (Breg) population was unveiled in human peripheral blood mononuclear cells and suggested as possible regulatory B cells (Breg) as regulatory T cells (Treg). The immunologic and the clinical relevant of Breg needs to be further investigated.

• BMB Reports
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• 제53권12호
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• pp.628-633
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• 2020

WNT11 is a member of the non-canonical Wnt family and plays a crucial role in tumor progression. However, the regulatory mechanisms underlying WNT11 expression are unclear. Tumor necrosis factor-alpha (TNFα) is a major inflammatory cytokine produced in the tumor microenvironment and contributes to processes associated with tumor progression, such as tumor invasion and metastasis. By using site-directed mutagenesis and introducing a serial deletion in the 5'-regulatory region of WNT11, we observed that TNFα activates the early growth response 1 (EGR1)-binding sequence (EBS) in the proximal region of WNT11 and that the transcription factor EGR1 is necessary for the TNFα-induced transcription of WNT11. EGR1 bound directly to the EBSs within the proximal 5'-regulatory region of WNT11 and ectopic expression of EGR1 stimulated WNT11 promoter activity, whereas the knockdown of EGR1 expression by RNA interference reduced TNFα-induced WNT11 expression in T47D breast cancer cells. We also observed that mitogen-activated protein kinases (MAPK), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 kinase mediated TNFα-induced transcription of WNT11 via EGR1. Our results suggest that EGR1 directly targets WNT11 in response to TNFα stimulation in breast cancer cells.

• 한국어병학회지
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• 제34권1호
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• pp.23-29
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• 2021

Interferon regulatory factors (IRFs) are a family of transcription factors essential to the control of antiviral immune response, cell growth, differentiation and apoptosis. IRF10 of zebrafish (Danio rerio) was negative regulation of the interferonΦ1 and 3 response in vitro. In this study, we analyze the induction of in vivo immune response activation from the IRF10 gene of zebrafish and the protective effect against VHSV. As the results, the group inoculated with IRF10 expression vectors, there was no expression of IFNΦ1, suggestion that IRF10 may function as a negative regulator of IRF3, which binds to the IFNΦ1 promoter. And other types of interferon genes (IFNΦ2-4) are thought to have been activated, inducing to the expression of pro-inflammatory cytokine and Mx genes. As the results of challenge test performed at 14 days after inoculation of the expression vectors, the maximum survival rate [50% (1㎍ DNA) and 42.5% (10㎍ DNA)] for IRF10 group were recorded. Meanwhile, the survival rates of pcDNA3.1 and PBS as the control groups were 10% and 15%, respectively. This study suggests that the possibility that activation of IRF10 molecule could be exploited as a VHS control method.

• Journal of Ginseng Research
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• 제41권2호
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• pp.127-133
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• 2017

Background: Ginsenoside Rc (G-Rc) is one of the major protopanaxadiol-type saponins isolated from Panax ginseng, a well-known medicinal herb with many beneficial properties including anticancer, anti-inflammatory, antiobesity, and antidiabetic effects. In this study, we investigated the effects of G-Rc on inflammatory responses in vitro and examined the mechanisms of these effects. Methods: The in vitro inflammation system used lipopolysaccharide-treated macrophages, tumor necrosis $factor-{\alpha}/interferon-{\gamma}-treated$ synovial cells, and HEK293 cells transfected with various inducers of inflammation. Results: G-Rc significantly inhibited the expression of macrophage-derived cytokines, such as tumor necrosis $factor-{\alpha}$ and $interleukin-1{\beta}$. G-Rc also markedly suppressed the activation of TANK-binding kinase $1/I{\kappa}B$ kinase ${\varepsilon}/interferon$ regulatory factor-3 and p38/ATF-2 signaling in activated RAW264.7 macrophages, human synovial cells, and HEK293 cells. Conclusion: G-Rc exerts its anti-inflammatory actions by suppressing TANK-binding kinase $1/I{\kappa}B$ kinase ${\varepsilon}/interferon$ regulatory factor-3 and p38/ATF-2 signaling.

• 한국콘텐츠학회논문지
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• 제15권6호
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• pp.215-226
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• 2015

본 연구에서는 합리적 행동이론을 적용하여 주차규제정책에 대한 태도, 주관적 규범, 집행기관에 대한 신뢰가 직접적으로 주차규제정책에 대한 행동의도와 행동에 어떠한 영향을 미치는지와 간접적으로 행동의도를 매개로 하여 행동에 어떠한 영향을 미치는지를 분석하고, 거주지역과 주택종류에 따른 조절효과를 검증하고자 하였다. 이를 위해 주차규제정책이 적용되고 있는 지역의 정책대상집단을 대상으로 설문조사하고 실증분석하였다. 구조방정식모형에 의한 가설검정결과, 주차규제정책에 대한 태도와 주관적 규범 요인이 행동의도에 각각 정(+)의 영향을 미치는 것으로 나타났으며, 행동에는 태도와 집행기관 요인이 정(+)의 영향을 미치는 것으로 나타났다. 그리고 행동의도를 매개로 하여 행동에도 각각 유의한 영향을 미치는 것으로 나타났다. 주거지역과 주택종류에 따른 조절효과를 검증한 결과, 주거지역에서는 태도와 행동의도 간의 관계에, 주택종류에서는 집행기관과 행동간에 관계에 따른 조절효과는 유의한 것으로 나타났다. 이러한 분석 결과를 토대로 본 연구에서는 주차규제정책에 대한 순응과 불응을 위한 실천적 방향을 제시하였다.

• 자원ㆍ환경경제연구
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• 제11권2호
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• pp.233-260
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• 2002

This paper tries to analyze the efficiency structure and regulatory effects in electric power industry by using total factor productivity and indices based on cost function method. This paper performed an empirical analysis on the efficiency improvement and optimal scale taking the cost aspect in electricity industry into account. By expanding the cost structure framework, this paper proposes the analytical method regarding the needs of technology regulation and presents the empirical results which can be verified. Hence, this work can play a key role in decision-making of the national power development and regulatory policy. The empirical result indicates that the electricity industry in Korea has been in the state of economies of scale until 1980s, However, due to sustained growth of power generation, economies of scale declined and subsided after 1980s and then diseconomies of scale is shown recent years. The analysis on the effect of technology regulation shows the national large-scale base-load power plant development-oriented policy until so far contributed substantial cost effect to the electricity industry. The empirical result indicates that the reserve requirement as one of means of technology regulation policy did not contribute to the economies of scale but positive effect on the total factor productivity in Korea.