Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Immune gene expression and protection effect against VHSV by injection of interferon regulatory factor 10 in zebrafish (Danio rerio)

제브라피쉬 interferon regulatory factor 10의 주사에 따른 면역 유전자 발현과 VHSV에 대한 방어 효과

  • Kim, Hye Ji (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Kim, Jin Young (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Park, Jong Bin (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Lee, Ji Hyun (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Park, Jeong Su (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Kim, Hyoung Jun (OIE Reference Laboratory for VHS, National Institute of Fisheries Science) ;
  • Kwon, Se Ryun (Department of Aquatic Life Medical Sciences, Sunmoon University)
  • 김혜지 (선문대학교 수산생명의학과) ;
  • 김진영 (선문대학교 수산생명의학과) ;
  • 박종빈 (선문대학교 수산생명의학과) ;
  • 이지현 (선문대학교 수산생명의학과) ;
  • 박정수 (선문대학교 수산생명의학과) ;
  • 김형준 (VHS OIE 표준실험실, 국립수산과학원) ;
  • 권세련 (선문대학교 수산생명의학과)
  • Received : 2021.05.28
  • Accepted : 2021.06.08
  • Published : 2021.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Interferon regulatory factors (IRFs) are a family of transcription factors essential to the control of antiviral immune response, cell growth, differentiation and apoptosis. IRF10 of zebrafish (Danio rerio) was negative regulation of the interferonΦ1 and 3 response in vitro. In this study, we analyze the induction of in vivo immune response activation from the IRF10 gene of zebrafish and the protective effect against VHSV. As the results, the group inoculated with IRF10 expression vectors, there was no expression of IFNΦ1, suggestion that IRF10 may function as a negative regulator of IRF3, which binds to the IFNΦ1 promoter. And other types of interferon genes (IFNΦ2-4) are thought to have been activated, inducing to the expression of pro-inflammatory cytokine and Mx genes. As the results of challenge test performed at 14 days after inoculation of the expression vectors, the maximum survival rate [50% (1㎍ DNA) and 42.5% (10㎍ DNA)] for IRF10 group were recorded. Meanwhile, the survival rates of pcDNA3.1 and PBS as the control groups were 10% and 15%, respectively. This study suggests that the possibility that activation of IRF10 molecule could be exploited as a VHS control method.

Keywords

Acknowledgement

이 논문은 국립수산과학원 수산과학연구사업(R2021065)의 지원 및 2017년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업(NRF-2017R1D1A1A0900992)임.

References

  1. Aggad, D., Mazel, M., Boudinot, P., Mogensen, K. E., Hamming, O. J., Hartmann, R., Kotenko, S., Herbomel, P., Lutfalla, G., and Levraud, J.-P.: The Two Groups of Zebrafish Virus-Induced Interferons Signal via Distinct Receptors with Specific and Shared Chains. The Journal of Immunology, 183:3924-3931, 2009. https://doi.org/10.4049/jimmunol.0901495
  2. de Kinkelin, P., and Dorson, M.: Interferon production in rainbow trout (Salmo gairdneri) experimentally infected with Egtved virus. The Journal of General Virology, 19:125-127, 1973. https://doi.org/10.1099/0022-1317-19-1-125
  3. Gomez-Casado, E., Estepa, A., and Coll, J. M.: A comparative review on European-farmed finfish RNA viruses and their vaccines. Vaccine, 29:2657-2671, 2011. https://doi.org/10.1016/j.vaccine.2011.01.097
  4. Haller, O., Staeheli, P., Schwemmle, M., and Kochs, G.: Mx GTPases: Dynamin-like antiviral machines of innate immunity. Trends in Microbiology, 23:154-163, 2015. https://doi.org/10.1016/j.tim.2014.12.003
  5. Huang, B., Li, W. X., Wang, Z. X., Liang, Y., Huang, W. S., and Nie, P.: Identification of a novel splice variant isoform of interferon regulatory factor 10, IRF10, in orange spotted grouper Epinephelus coioides. Fish & Shellfish Immunology, 97:637-647, 2020. https://doi.org/10.1016/j.fsi.2019.12.056
  6. Huang, Bei, Qi, Z. T., Xu, Z., and Nie, P.: Global characterization of interferon regulatory factor (IRF) genes in vertebrates: Glimpse of the diversification in evolution. BMC Immunology, 11:1-28, 2010. https://doi.org/10.1186/1471-2172-11-1
  7. Kim, H. J., Park, J. S., Choi, M. C., and Kwon, S. R.: Comparison of the efficacy of Poly(I: C) immunization with live vaccine and formalin-killed vaccine against viral hemorrhagic septicemia virus (VHSV) in olive flounder (Paralichthys olivaceus). Fish and Shellfish Immunology, 48:206-211, 2016. https://doi.org/10.1016/j.fsi.2015.11.035
  8. Komuro, A., Bamming, D., and Horvath, C. M.: Negative regulation of cytoplasmic RNA-mediated anti- viral signaling. Cytokine, 43:350-358, 2008. https://doi.org/10.1016/j.cyto.2008.07.011
  9. Langevin, C., Aleksejeva, E., Passoni, G., Palha, N., Levraud, J. P., and Boudinot, P.: The antiviral innate immune response in fish: Evolution and conservation of the IFN system. Journal of Molecular Biology, 425:4904-4920, 2013. https://doi.org/10.1016/j.jmb.2013.09.033
  10. Li, S., Lu, L.-F., Feng, H., Wu, N., Chen, D.-D., Zhang, Y.-B., Gui, J.-F., Nie, P., and Zhang, Y.-A.: IFN Regulatory Factor 10 Is a Negative Regulator of the IFN Responses in Fish. The Journal of Immunology, 193:1100-1109, 2014. https://doi.org/10.4049/jimmunol.1400253
  11. Loo, Y.-M., Fornek, J., Crochet, N., Bajwa, G., Perwitasari, O., Martinez-Sobrido, L., Akira, S., Gill, M. A., Garcia-Sastre, A., Katze, M. G., and Gale, M.: Distinct RIG-I and MDA5 Signaling by RNA Viruses in Innate Immunity. Journal of Virology, 82:335-345, 2008. https://doi.org/10.1128/JVI.01080-07
  12. Lopez-Munoz, A., Roca, F. J., Meseguer, J., and Mulero, V.: New Insights into the Evolution of IFNs: Zebrafish Group II IFNs Induce a Rapid and Transient Expression of IFN-Dependent Genes and Display Powerful Antiviral Activities. The Journal of Immunology, 182:3440-3449, 2009. https://doi.org/10.4049/jimmunol.0802528
  13. Lu, M. W., Chao, Y. M., Guo, T. C., Santi, N., Evensen, O., Kasani, S. K., Hong, J. R., and Wu, J. L.: The interferon response is involved in nervous necrosis virus acute and persistent infection in zebrafish infection model. Molecular Immunology, 45:1146-1152, 2008. https://doi.org/10.1016/j.molimm.2007.07.018
  14. Ozato, K., Tailor, P., and Kubota, T.: The interferon regulatory factor family in host defense: Mechanism of action. Journal of Biological Chemistry, 282:20065-20069, 2007. https://doi.org/10.1074/jbc.R700003200
  15. Stark, G. R.: How cells respond to interferons revisited: From early history to current complexity. Cytokine and Growth Factor Reviews, 18:419-423, 2007. https://doi.org/10.1016/j.cytogfr.2007.06.013
  16. Stein, C., Caccamo, M., Laird, G., and Leptin, M.: Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish. Genome Biology, 8, 2007.
  17. Sun, F., Zhang, Y.-B., Liu, T.-K., Shi, J., Wang, B., and Gui, J.-F.: Fish MITA Serves as a Mediator for Distinct Fish IFN Gene Activation Dependent on IRF3 or IRF7. The Journal of Immunology, 187:2531-2539, 2011. https://doi.org/10.4049/jimmunol.1100642
  18. Tonheim, T. C., Bogwald, J., and Dalmo, R. A.: What happens to the DNA vaccine in fish? A review of current knowledge. Fish and Shellfish Immunology, 25:1-18, 2008. https://doi.org/10.1016/j.fsi.2008.03.007
  19. Turner, M. D., Nedjai, B., Hurst, T., and Pennington, D. J.: Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease. Biochimica et Biophysica Acta - Molecular Cell Research, 1843:2563-2582, 2014. https://doi.org/10.1016/j.bbamcr.2014.05.014
  20. Xu, Q., Jiang, Y., Wangkahart, E., Zou, J., Chang, M., Yang, D., Secombes, C. J., Nie, P., and Wang, T.: Sequence and Expression Analysis of Interferon Regulatory Factor 10 (IRF10) in Three Diverse Teleost Fish Reveals Its Role in Antiviral Defense. PLoS ONE, 11:1-22, 2016.