• Animal Bioscience
• /
• v.37 no.6
• /
• pp.1007-1020
• /
• 2024

Objective: We increased the nuclear maturation rate of antral follicle derived oocytes by using a pre-in vitro maturation (IVM) culture system and improved the developmental potential of these porcine pathenotes by supplementing with melatonin. Furthermore, we investigated the expression patterns of genes involved in cumulus expansion (HAS2, PTGS2, TNFAIP6, and PTX3) derived from small and medium antral follicles before and after oocyte maturation. Methods: Only the cumulus oocyte-complexes (COCs) derived from small antral follicles were induced with [Pre-SF(+)hCG] or without [Pre-SF(-)hCG] the addition of human chorionic gonadotropin (hCG) during the last 7 h of the pre-IVM period before undergoing the regular culture system. The mature oocytes were investigated on embryonic development after parthenogenetic activation (PA). Melatonin (10^-7 M) was supplemented during in vitro culture (IVC) to improve the developmental potential of these porcine pathenotes. Results: A pre-IVM culture system with hCG added during the last 7 h of the pre-IVM period [Pre-SF(+)hCG] effectively supported small antral follicle-derived oocytes and increased their nuclear maturation rate. The oocytes derived from medium antral follicles exhibited the highest nuclear maturation rate in a regular culture system. Compared with oocytes cultured in a regular culture system, those cultured in the pre-IVM culture system exhibited considerable overexpression of HAS2, PTGS2, and TNFAIP6. Porcine embryos treated with melatonin during IVC exhibited markedly improved quality and developmental competence after PA. Notably, melatonin supplementation during the IVM period can reduce and increase the levels of intracellular reactive oxygen species (ROS) and glutathione (GSH), respectively. Conclusion: Our findings indicate that the Pre-SF(+)hCG culture system increases the nuclear maturation rate of small antral follicle-derived oocytes and the expression of genes involved in cumulus expansion. Melatonin supplementation during IVC may improve the quality and increase the blastocyst formation rate of porcine embryos. In addition, it can reduce and increase the levels of ROS and GSH, respectively, in mature oocytes, thus affecting subsequent embryos.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.40 no.9
• /
• pp.1776-1785
• /
• 2015

Content Centric Network (CCN) has been introduced as a new paradigm due to a shift of users's perspective of using Internet from host-centric to content-centric. On the other hand, a demand for video streaming has been increasing. Thus, Adaptive streaming has been introduced and researched for achieving higher user's satisfaction. If an architecture of Internet is replaced with CCN architecture, it is necessary to consider adaptive video streaming in CCN according to the demand of users. However, if the same rate decision algorithm used in Internet is deployed in CCN, there are a limitation of utilizing content store (CS) in CCN router and a problem of reflecting dynamic requirements. Therefore, this paper presents a framework adequate to CCN protocol and cache utilization, adapting content naming method of exploiting regular expression to the rate decision algorithm of the existing adaptive streaming. In addition, it also improves the quality of video streaming and verifies the performance through dynamic expression strategies and selection algorithm of the strategies.

• Korean Journal of Veterinary Research
• /
• v.43 no.3
• /
• pp.449-456
• /
• 2003

The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein.

• Journal of Nutrition and Health
• /
• v.34 no.7
• /
• pp.727-733
• /
• 2001

The present study was conducted to identify the mechanism about the regulation of appetite by examining the expression patterns of neuropeptide Y in the hypothalamus of mice either fasting mouse for 24 hours or with anorexia mutant mouse. In order to investigate the patterns of expression of neurpeptide Y, immunohistochemistry was employed for measurements at the tissue level, along with the molecular biological techniques of reverse transcription polymerase chain reaction(RT-PCR) and dot blotting. The results of this study are as follows. The level of expression of neruopeptide Y, a neuropeptide known to enhance appetite, was shown to be lowered in the arcuate nucleus(ARC), paraventricular nucleus(PVN), lateral hypothalamic area(LHA), and dorsomedial hypothalamic nucleus(DMN) in both the fasting and anorexia mutant groups when measured via immunohistochemistry, a tissue-level method. RT-PCR and dot blotting, the molecular biological methods employed in this study, revealed that the level of neuropeptide Y mRNA in the entire hypothalamus was similar in the control and fasting groups and lower in the anorexia mutant group. The results of the present study showed that while the levels of expression of the neuropeptide Y in the various hypothalamic regions studied did not exhibit regular increases or decreases when measured immunohistochemically. But the entire hypothalamus via molecular biological methods showed that the changes in these levels were more definite in the anorexia mutant group than in the fasting group.

• International Journal of Internet, Broadcasting and Communication
• /
• v.13 no.4
• /
• pp.11-21
• /
• 2021

The causes of noise generation according to the classification of indoor spaces are very diverse. Individual happiness is infringed by this noise. In this paper, We tried to visualize the spatial sound characteristics of public places using sound color to express them so that anyone can sympathize. The noise inside a conference room of a medical device company was measured for 100 minutes, and the frequency band was divided into three different types of existing sound pressure expression units. Because the size of the noise is expressed differently depending on the situation, There are cases where there is a difference of opinion between the measurer and the researcher. This noise measurement experiment was conducted, and the sound color was applied to classify it on a log scale considering auditory characteristics. As a result of comparing this with the result expression for different loudness expression units, A specific table in different units yielded almost similar results. In addition, the sound source section for 100 minutes was divided into three analysis sections, the analysis sections were different, and the size of the energy ratio for each analysis section was divided in the form of an envelope. The characteristics of the low-frequency region of the space have a high energy ratio, and the decrease in the energy ratio according to the increase in frequency is constant and regular. You can see that conversations are possible.

• International Journal of Control, Automation, and Systems
• /
• v.6 no.4
• /
• pp.515-525
• /
• 2008

In this paper, the problem of delay-dependent stabilization for singular systems with multiple internal and external incommensurate constant point delays is investigated. The condition when a singular system subject to point delays is regular independent of time delays is given and it can be easily test with numerical or algebraic methods. Based on Lyapunov-Krasovskii functional approach and the descriptor integral-inequality lemma, a sufficient condition for delay-dependent stability is obtained. The main idea is to design multiple memoryless state feedback control laws such that the resulting closed-loop system is regular independent of time delays, impulse free, and asymptotically stable via solving a strict linear matrix inequality (LMI) problem. An explicit expression for the desired memoryless state feedback control laws is also given. Finally, a numerical example illustrates the effectiveness and the availability for the proposed method.

• Journal of Life Science
• /
• v.21 no.7
• /
• pp.997-1003
• /
• 2011

Menopause and obesity are associated with metabolism. The purpose of this study was to examine the changes of PPAR${\gamma}$, PGC-1(${\alpha},\;{\beta}$), NRf-1 and TFAM mRNA and mitochondria biogenesis in adipocytes and investigate the effect of swimming exercise for 6weeks on ovariectomized rats. Rats were randomly assigned to 3 groups: (1) ovariectomized rats fed with a control diet (C, n=4), (2) ovariectomized rats fed with high fat diet (H, n=4), and (3) ovariectomized rats trained to exercise and fed with high fat diet (H+EX, n=4). Exercise was performed by swimming for 5 days/wk, with a progressive increase in exercise over the course of 6 weeks. Results showed that the fat tissue weight in the H group was markedly increased (p<0.01) compared to other groups, however, regular exercise significantly decreased fat weight. The PPAR-${\gamma}$ (p<0.05), PGC-$1{\alpha}$ (p<0.01), -$1{\beta}$ (p<0.05), NRf-1 (p<0.01) and TFAM (p<0.05) mRNA expression in the adipocytes of H+EX were higher than in the H group. These results suggest that regular exercise for 6 weeks might exert positive effects by increasing PPAR-${\gamma}$, PGC-1 (${\alpha},\;{\beta}$), NRf-1 and TFAM mRNA expression and mitochondria in adipocytes. Thus, regular exercise may be helpful in the improvement of mitochondria biogenesis function in obese, ovariectomized rats.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.22 no.4
• /
• pp.81-86
• /
• 2022

In this work, we propose a method for automatically determining the domain of columns of file data structured by csv format. New data can be generated through convergence between data and data, and the consistency of the joined columns must be maintained in order for these new data to become an important resource. One of the methods for measuring data quality is a domain-based quality diagnosis method. Domain is the broadest indicator that defines the nature of each column, so a method of automatically determining it is necessary. Although previous studies mainly studied domain automatic discrimination of relational databases, this study developed a model that can automate domains using the characteristics of file data. In order to specialize in the domain discrimination of file data, the data were simplified and patterned using a regular expression, and the contents of the data header corresponding to the column name were analyzed, and the suffix used was used as a derived variable. When derivatives of regular expressions and suffixes were added, the result of automatically determining the domain with an accuracy of 95% greater than the existing method of 87% was derived. This study is expected to reduce the quality measurement period and number of people by presenting an automation methodology to the quality diagnosis of public data.

• Journal of Radiation Industry
• /
• v.5 no.1
• /
• pp.47-54
• /
• 2011

In this study, we investigated to evaluate differential expression of genes encoding lipid transfer proteins (LTP) by acute and chronic gamma irradiation in rice. After acute and chronic gamma irradiation by 100 Gy and 400 Gy to rice plant, necrotic lesion was observed in the leaf blade and anthocyanin contents were increased. We isolated a total of 21 rice lipid transfer protein (LTP) genes in the TIGR database, and these genes were divided into four different groups on the basis of nucleotide sequences. The LTP genes also were classified as different four classes according to expression pattern using RT-PCR. Group A, B contained genes with increased expression and decreased expression in acute and chronic, respectively. Group C contained genes with contrasted expression pattern. Group D wasn't a regular pattern. But the specific affinity was not obtained between two grouping.

• Journal of KIISE:Databases
• /
• v.30 no.6
• /
• pp.641-650
• /
• 2003

The eXtensible Markup Language(XML) is emerging as a standard format of data representation and exchange on the Internet. There have been researches about storing and retrieving XML documents using the relational database which has techniques in full growth about large data processing, recovery, concurrency control and so on. Since in previous systems same structure information and fundamental operation are used for processing of various kinds of XML queries, only some specific query can be efficiently processed not all types of query. In this paper, we propose a flexible query processing system. To process query efficiently, the proposed system analyzes regular path expression queries, and uses $\theta$-join operation using region numbering values to check ancestor-descendent relationship and equi-join operation using parent's region start value to check parent-child relationship. Thus, the proposed system processes efficiently XML regular path expressions. From the experimental results, we show that proposed XML query processing system is more efficient than previous systems.