• 한국산림과학회지
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• 제96권1호
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• pp.34-39
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• 2007

잡종키위(Actinidia deliciosa${\times}$A. arguta) 엽조직의 캘러스로부터 완전한 식물체를 재분화시킬 수 있었다. 캘러스는 옥신류(2,4-D, NAA: 0.1~0.5 mg/l)와 싸이토키닌류(BA: 0.1~0.2 mg/l)를 조함하여 첨가된 MS배지에 엽절편체를 배양하여 캘러스를 유도하였다. 가장 높은 캘러스 유도율(96.2%)은 0.5 mg/1 2,4-D+0.1 mg/l NAA+0.05 mg/l BA의 처리구에서 나타났다. 신초유도는 1차 신초 유도배지에서 1.0 mg/l BA+0.05 mg/l IBA 혹은 2.0 mg/l BA+0.05 mg/l lBA 첨가구에서만 소수의 신초가 유도되었다. 그러나 캘러스를 1.0, 2.0 혹은 5.0 mg/l zeatin의 단독처리구 혹은 BA, TDZ 혹은 zeatin이 첨가된 2차 신초 유도배지처리구에서 다경유도가 가능하였지만 BA 첨가구에는 그 농도에 관계없이 다경유도는 이루어지지 않아 잡종키위의 캘러스로부터 신초유도시 BA첨가는 별 효과가 없는 것으로 나타났다. 신초로부터 발근은 기내발근 [Standardi (St)+1.0 mg/l IBA]시 가장 높은 발근율(83.3%)를 보였지만 500 mg/l IBA의 수용액에 신초를 1시간동안 침지한 처리구에서 가장 저조한 발근율(40.0%)을 보였다. 줄기와 뿌리가 유도된 완전한 식물체를 재분화가 가능하였고 순화 또한 성공적으로 이루어졌다.

• Journal of Plant Biotechnology
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• 제7권1호
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• pp.57-65
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• 2005

The $F_1$ hybrid Red Coat is one of the most highly sought after cultivars of tomato in Australia and yields up to 7.5 $\cal{kg/plant}$. An experiment was conducted to de-termine the optimal strength and type of growth medium and sucrose concentration for shoot organogenesis of the Red Coat cultivar using cotyledonary explants. Two basal growth media, viz. MS and Gamborg' s $B_5$ at 0, 1/4, 1/2, full or double strength along with sucrose concentrations of 0, 0.5, 1.5, 3 or $5\%$, were evaluated using 25 replications. The main effects of treatment and their mutual interactions were evaluated for the proportion of explants that produced callus and/or shoots, number of shoots produced per explant, callus diameter and shoot height. The explants failed to produce shoots in the absence of mineral nutrient. Only a small proportion of the explants ($6\%$ with $B_5\;and\;3\%$ with MS) regenerated shoots in the absence of sucrose. Lower sucrose concentrations ($0.5-1.5\%$) along with full strength media were optimal for most of the traits studied. The $B_5$ medium outperformed MS medium for shoot organogenesis. For all the traits examined, significant differences in main effects (P < 0.05) and two-way interactions were detected, but no three-way interactions (medium type $\times$ medium concentration $\times$ sucrose concentration) were observed. Sucrose was found essential for the development of chlorophyll. Chlorophyll content increased with an increase in sucrose concentration up to $3\%$ and decreased at $5\%$ sucrose.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.221-224
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• 2003

Leaf explants of Ixeris sonchifolia produced adventitious shoots at a frequency of 100% when cultured on MS medium supplemented with combinations of various concentrations of 6-benzyladenine (BA) (0.44, 4.44, or 8.87 ${\mu}M$) and 0.54 ${\mu}M$ NAA, or MS medium supplemented with 22.19 ${\mu}M$ BA and 2.69 ${\mu}M\;\alpha$-naphthaleneacetic acid (NAA) after four weeks of culture. Each explants (approximately $3{\times}6mm$) produced greater than 70 shoots at a combination of 0.44 ${\mu}M$ BA and 0.54 ${\mu}M$ NAA. Leaf explants produced shoots at a frequency of greater than 80% even at as low as 0.13 ${\mu}M$ BA as the sole growth regulator. Upon transfer to one-third strength MS with 0.54 ${\mu}M$ NAA, excised adventitious shoots were rooted at a frequency of 100%. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse. The competence of I. sonchifolia for plant regeneration via organogenesis appears to be greater than the competence of tobacco, currently the best model plant for organogenesis.

• Journal of Plant Biotechnology
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• 제47권1호
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• pp.40-45
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• 2020

An efficient protocol for multiple shoot induction and plant regeneration from axillary bud culture of Magnolia 'Vulcan' was developed in the present study. Primary shoots were obtained from axillary bud explants cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BA). To induce multiple shoots effectively, primary shoot tips were cultured on MS medium supplemented with different concentrations of BA and zeatin at 0, 0.2, 0.5, and 1.0 mg/L. Of these treatments, the MS medium with 0.5 mg/L BA resulted in the highest number of shoots per explant with an average value of 5.9, and it produced the greatest shoot height at 4.8 cm after 12 weeks of culturing. In the rooting of in vitro produced shoots, the greatest percentage of explants forming roots (91.3%), number of roots per explant (9.7), and root length (2.8 cm) were obtained in half-strength MS medium supplemented with 6.0 mg/L indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room with 87.5% survival rate. Plants were transferred to a greenhouse with a 97.2% survival rate. The highly efficient shoot multiplication and plant regeneration system reported herein can be used for large-scale clonal propagation of valuable Magnolia species or cultivars.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.27-35
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• 2006

Plantlet regeneration in Asteracantha longifolia(L.) Nees (Acanthaceae), a medicinal herb has been achieved from seedling explants on basal MS medium. Three different seedling explants including node, internode and leaf segments on used. Of these three explant, leaf explants gave better response for both callus mediated organogenesis and direct multiple shoot induction. Number of explants showing differentiation of shout buds was higher on MS media supplemented with BA compared to kinetin. MS medium fortified with BA ($2.0mgl^{-1}$) and NAA ($0.5mgl^{-1}$) was found to be most suitable for both callus mediated organogenesis and elongation of shouts. The elongated shoots were successfully routed on MS medium fortified with NAA or IBA. Among them $0.1mgl^{-1}$ NAA or $0.2mgl^{-1}$ IBA provides better response for rhizogenesis. Regenerated plantlets were successfully established in soil where 85.4% or them developed into morphologically normal and fertile plants. RAPD profiling using four decamer primers confirmed the genetic uniformity of the regenerated plantlets and substantiated the efficacy and suitability of this protocol for in vitro propagation of A. longifolia.

• Plant Biotechnology Reports
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• 제5권3호
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• pp.255-263
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• 2011

Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain several kinds of triterpenoid saponin with high medicinal values. In this work, we induced transgenic hairy roots of C. lanceolata and analyzed triterpenoid saponins from the hairy roots and hairy root-derived transgenic plants. Hairy roots were obtained from leaf explants by the transformation of Agrobacterium rhizogenes R1000. Transgenic hairy root lines were confirmed by the transcriptional activities of rolA, B, C, and D genes by RT-PCR. Transgenic root lines actively proliferated on hormone-free medium but not in nontransformed roots. Hairy roots contained richer triterpenoids (lancemaside A, foetidissimoside A, and aster saponin Hb) than nontransformed roots. Transgenic plants were regenerated from the hairy roots via somatic embryogenesis. They showed phenotypic alterations such as shortened shoots and an increased number of axillary buds and adventitious roots. The transgenic plants also contained higher triterpenoid levels than wild-type plants. These results suggest that hairy roots and transgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.267-272
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• 2005

Common bean (Phaseolus vulgaris L.) plants were regenerated via organogenesis from mature embryonic axes, cultured on MS medium supplemented with ildole-3-ecetic acid (IAA) and thidiazuron (TDZ) for one week in the dark. Embryonic axillary regions were excised, longitudinally cut to split the both sides, and cultured for two weeks on MS medium supplemented with IAA and TDZ. The combination 0.5 mg $l^{-1}$ TDZ/0.5 mg $l^{-1}$ IAA presented the higher efficiency in shoot regeneration and the combination 0.5 mg $l^{-1}$ TDZ/0.25 mg $l^{-1}$ IAA presented the higher efficiency in conversion of shoots to plants. Regenerating explants were transferred to MS medium containing 1 mg $l^{-1}$ BAP for shoot development. All elongated shoots were rooted in vitro, presented normal phenotype and produced viable seeds. Histological analysis confirmed the mode of regeneration as de novo shoot organogenesis.

• Journal of Plant Biotechnology
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• 제35권3호
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• pp.209-213
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• 2008

This study was conducted to regenerate shoots from internode sections(about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body(PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25 $mg{\cdot}L^{-1}$ thidiazuron(TDZ) and 0.5 $mg{\cdot}L^{-1}$ indole-3-butyric acid(IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.

• 한국작물학회지
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• 제47권5호
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• pp.352-355
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• 2002

Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration. Murashige and Skoog(MS) and N6 media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin were used for callus induction. Percentage of callus induction showed significant among the combinations of plant growth regulators. Callus induction showed high efficiency in medium containing 3 mg/$\ell$ of 2,4-D. The high frequency of callus induction was obtained in Gwiri37. For plant regeneration, calli induced from mature embryos were transferred onto MS and N6 media supplemented with combinations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) for 5 weeks. Percentage of plant regeneration showed high in MS medium containing 0.2 mg/$\ell$ of NAA and 1 mg/$\ell$ of BA. The callus initiation medium affected the subsequent plant regeneration. Treatment with 3 mg/$\ell$ of 2,4-D, and 3 mg/$\ell$ of 2,4-D and 3 mg/$\ell$ of kinetin in callus induction media showed high frequency for plant regeneration. Plant regeneration frequency among the genotypes showed significant. Especially, Gwiri37 showed high regeneration frequency. Regenerated shoots were treated with 200, 350 and 500 mg/$\ell$ of indole-3-butyric acid (IBA) transferred onto half-strength MS medium without plant growth regulators. Treatment of shoots with IBA induced root formation rapidly.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.101-107
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• 2010

An efficient plant regeneration system has been developed for figleaf gourd (Cucurbita ficifolia Bouch$\'{e}$), which is exclusively used as a rootstock for cucumber. The protocol is based on results obtained from a series of culture experiments involving different parts of the cotyledons and various media. The culture of cotyledon explants was critical for the enhancement of shoot regeneration frequency. The lower parts of the cotyledon excised at the plumule base were found to display a markedly enhanced production of adventitious shoots compared to other cotyledon regions. Culture in silver nitrate-supplemented Murashige and Skoog (MS) medium was not beneficial for shoot regeneration and suppressed root regeneration. Efficient shoot regeneration was obtained on MS medium containing 1.0 $mg\;l^{-1}$ zeatin and 0.1 $mg\;l^{-1}$ indole-3-acetic acid. Regenerated shoots successfully elongated and rooted in medium containing 0.1 $mg\;l^{-1}$ 1-naphthalene-acetic acid after 10-15 days of subculturing. The plantlets were satisfactorily acclimatized in a greenhouse and grew into normal plants without any morphological alterations.