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Characterization of a Novel cry1-Type Gene from Bacillus thuringiensis subsp. alesti Strain LY-99

  • Qi, Xu Feng;Li, Ming Shun;Choi, Jae-Young;Roh, Jong-Yul;Song, Ji Zhen;Wang, Yong;Jin, Byung-Rae;Je, Yeon-Ho;Li, Jian Hong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.1
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    • pp.18-27
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    • 2009
  • B. thuringiensis strain LY-99 belonging to subsp. alesti (H3a3c), was isolated from Chinese tobacco warehouse and showed significantly high toxicity to Plutella xylostella. For the identification of the cry1-type genes from B. thuringiensis LY-99, an extended multiplex PCRrestriction fragment length polymorphism (PCRRFLP) method was established by using two pairs of universal primers based on the conserved regions of the cry1-type genes to amplify around 2.4 kb cry1-type gene fragments. Then the DNA fragment was cloned into pGEM-T Easy vector and digested with EcoRI and EcoRV enzymes. Through this method, a known cry1-type gene was successfully identified from the reference strain, B. thuringiensis subsp. alesti. In addition, the RFLP patterns revealed that B. thuringiensis LY-99 included a novel cry1A-type gene in addition to cry1Aa, cry1Ac, cry1Be and cry1Ea genes. The novel cry1A-type gene was designated cry1Ah2 (Genbank accession No DQ269474). An inverse PCR method was used to amplify the flank regions of cry1Ah2 gene. Finally, 3143 bp HindIII fragment from B. thuringiensis LY-99 plasmid DNA including 5' region and partial ORF was amplified, and sequence analysis revealed that cry1Ah2 gene from LY-99 showed 89.31% of maximum sequence similarity with cry1Ac1 crystal protein gene. In addition, the deduced amino acid sequence of Cry1Ah2 protein shared 87.80% of maximum identity with that of Cry1Ac2. This protein therefore belongs to a new class of B. thuringiensis crystal proteins.

Understanding of Percentages of Sixth Grade Students in Elementary School (초등학교 6학년 학생의 백분율 이해에 관한 연구)

  • Lee, Soo Eun;Chong, Yeong Ok
    • Journal of Elementary Mathematics Education in Korea
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    • v.21 no.2
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    • pp.309-341
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    • 2017
  • This study aims to investigate an approach to teach percentages in elementary mathematics class by analyzing calculating strategies with percentage the students use to solve the percentage tasks and their percentages of correct answers, as well as types of errors with percentages the students make. For this research 182 sixth graders were examined. The instrument test consists of various task types in reference to the previous study; the percentages tasks are divided into algebraic-geometric, part whole-comparison-change and find part-find whole-find percentage tasks. According to the analysis of this study, percentages of correct answers of students with percentage tasks were lower than we expected, approximately 50%. Comparing the percentages of correct answers according to the task types, the part-whole tasks are higher than the comparison and change tasks, the geometric tasks are approximately equal to the algebraic tasks, and the find percentage tasks are higher than the find whole and find part tasks. As to the strategies that students employed, the percentage of using the formal strategy is not much higher than that of using the informal strategy, even after learning the formal strategy. As an insightful approach for teaching percentages, based on the study results, it is suggested to reinforce the meaning of percentage, include various types of the comparison and change tasks, emphasize the informal strategy explicitly using models prior to the formal strategy, and understand the relations among part, whole and percentage throughly in various percentage situations before calculating.

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An Analysis of Success Factors in Internet Shopping Malls (인터넷 쇼핑몰구축의 성공요인에 대한 분석)

  • 진영배;권영식
    • Journal of the Korea Computer Industry Society
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    • v.2 no.12
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    • pp.1495-1504
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    • 2001
  • The main purpose of this research is to show Internet shopping mall the strategic way with the analysis of success factors in Internet shopping malls. To achieve the above purpose, the success factors and variables were defined by the survey of the reference. Sample malls for the research un selected of shopping malls registered in yahoo, Lycos, Empas and Hanmir regardless of their type and class, and did an online-survey of their operation. From the above method, the following results are deduced. First, there are five factors in the success of Internet shopping malls: the effectiveness of customer management the effectiveness of marketing. the competitiveness of product-sales, the convenience of use, the credibility of product. Second, the effectiveness of marketing is positively related to the number of member, visitors, and sales. Third, the credibility of product is negatively related to the number of member, visitors, and sales. At the end, the number of member and visitor are positively related to sales. This result could provide the managers with highly relevant managerial issues. The implication of the study are discussed and futher research directions are proposed.

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문헌정보학과 WWW홈페이지의 필요성과 준비에 관한 연구- 한국과 북미주 지역 대학을 중심으로 -

  • 박일종
    • Journal of Korean Library and Information Science Society
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    • v.24
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    • pp.413-448
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    • 1996
  • Nowadays, the World Wide Web (WWW) has become an important resource of timely information for the information-related people such as information scientists, librarians, and students in Library and Information Sciences area. They are information professionals who navigate the information on the internet. Also, they need to be information providers who build a WWW homepage. This paper is a study of the necessity, preparation, and building WWW Homepage files for school of Library and Information Sciences in the age of competition among disciplines. It is particularly focused on the colleges and universities in Republic of Korea (ROK) and North America area. The purpose of this study is to provide various kinds of reference information to prepare a homepage in the future as utilizing information on the internet effectively. Even though a Web page was necessary for a school of Library and Information Sciences to show news, and introduce the purpose of the disciplines and the curriculum of the school, and the professors of a class etc., it was not well-prepared yet in ROK. However, a web page was used well enough and prosperous in North America area (Canada, the United States, and Puerto Rico) comparatively. Those web pages were analyzed and studied to prepare for a good designing of homepages for school of Library and Information Sciences in Korea and for the age of competition among disciplines in this paper. Suggestions for designing a good homepage and guidelines for preparing a best one were studied after both reviewing literature and utilizing experiences by the author who currently serves in the School of Library and Information Sciences in Keimyung University and builds homepage for the school. As a result, the major suggestions are ; premiered, and they are as follows: (1) English version of a homepage is necessary, (2) Provide a multimedia presentation about the nature of a school (3) Incorporate a place to let people make suggestions on the contents (of a homepage), (4) Bear in mind that potential users must be familiar with abbreviations you used, (5) Absolutely do not use abbreviations that may make the content more difficult to understand, (6) Add a feature on the every single page that will take the user back to the main page, (7) Use clear, short and well-structured sentences and remember to divide text into paragraphs, (8) Date with a creation or modification date in the homepage to indicate the updated date, (9) Being a multimedia environment, use colors effectively (the guidelines were also suggested), and (10) Put colleges' name into the title of bookmarks to find out them easily.

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A Study on Suppression of UT Grain Noise Using SSP MPO Algorithms (SSP MPO 알고리즘을 이용한 초음파 결정립 잡음 억제에 관한 연구)

  • Koo, Kil-Mo;Jun, Kye-Suk
    • The Journal of the Acoustical Society of Korea
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    • v.15 no.6
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    • pp.81-89
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    • 1996
  • It is very important for ultrasonic test method to evaluate the integrity of the class I components in nuclear power plants. However, as the rltrasonic test is affected by internal structures and configurations of test materials, backscattering, that is, time invariant noise is generated in large grain size materials. Due to the above reason, the received signal results in low signal to noise(S/N) ratio. Split spectrum processing(SSP) technique is effective to suppress the grain noise. The conventional SSP technique. however, has been applied to unique algorithm. This paper shows that MPO(minimization and polarity threshold) algorithm which two algorithms are applied simulatancously, was utilized, the signal processing time was shorten by using the new constant-Q SSP with the FIR filter which frequency to bandwidth ratio is constant and the optimum parameters were analysed for the signal processing to longitudinal wave and shear wave with the same requirements of inspection on nuclear power plant site. Moreover, the new ultrasonic test instrument, the reference block of the same product form and material specification, stainless stell test specimens and copper test specimens block of the same fabricated for the application of new SSP technique. As the result of experimental test with new ultrasonic test instrument and test specimens, the signal to noise ratio was improved by appying the new SSP technique.

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Bioequivalence of Losata Tablet to Cozaar Tablet (Losartan Potassium 50 mg) using HPLC with Fluorescence Detector (고속액체크로마토그래피-형광검출기를 이용한 코자 정(로자탄칼륨 50 mg)에 대한 로사타 정의 생물학적동등성)

  • Kang, Hyun-Ah;Kim, Se-Mi;Yoo, Dong-Jin;Kang, Seung-Rae;Lee, Sang-No;Kim, Yong-Hee;Lee, Yong-Bok
    • Journal of Pharmaceutical Investigation
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    • v.40 no.1
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    • pp.51-57
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    • 2010
  • Losartan potassium, 2-butyl-4-chloro-1-[p-(o-1H-tetrazol-5-ylphenyl)benzyl]imidazole-5-methanol mono-potassium salt, is a new class of antihypertensive agents, and is an antagonist in angiotensin receptor. The purpose of the present study was to evaluate the bioequivalence of two Losartan potassium tablets, Cozaar tablet (MSD Pharmaceutical Co., Ltd.) and Losata tablet (Kyung Dong Pharmaceutical Co., Ltd.), according to the guidelines of the Korea Food and Drug Administration (KFDA). The release of losartan from the two losartan potassium formulations in vitro was tested using KP VIII Apparatus II method with various dissolution media. Twenty eight healthy male subjects, $23.86{\pm}1.80$ years in age and $67.27{\pm}6.60\;kg$ in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After a single tablet containing 50 mg as losartan potassium was orally administered, blood samples were taken at predetermined time intervals, and the concentrations of losartan in serum were determined using HPLC with fluorescence detector. The dissolution profiles of two formulations were similar in all tested dissolution media. The pharmacokinetic parameters such as $AUC_t$, $C_{max}$ and $T_{max}$ were calculated, and Equiv Test/K-BE Test 2002 was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_t$, $C_{max}$ and untransformed $T_{max}$. The results showed that the differences between two formulations based on the reference drug, Cozaar, were -2.70%, 1.45% and 2.31% for $AUC_t$, $C_{max}$ and $T_{max}$, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log 0.8 to log 1.25 (e.g., log 0.8852~log 1.0655 and log 0.8319~log 1.2342 for $AUC_t$ and $C_{max}$, respectively). Thus, the criteria of the KFDA bioequivalence guideline were satisfied, indicating Losata tablet was bioequivalent to Cozaar tablet.

Factors Related to Dietary Fiber Intake Among Sixth Grade Elementary School Children in Daejon City

  • Chung, Young-Jin;Lee, Yangsoon;Suh, Yoon-Suk
    • Nutritional Sciences
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    • v.7 no.2
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    • pp.113-120
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    • 2004
  • This study was conducted to investigate variables related to dietary fiber intake among sixth grade children in an elementary school in Daejon city. One hundred and forty-seven children completed a questionnaire for determining their socioeconomic background and their food habits. Anthropometric measurements were taken, and a 24-hour diet recall method was used to collect three-day food intakes. The socioeconomic status of the children's families belonged to the upper middle class; 53.0% of their fathers and 25.8% of their mothers had completed college or higher degrees. 27.9% of the mothers had jobs, including part-time jobs. Approximately 30% of the children skipped breakfast, 66.4% of the children preferred animal foods to plant foods, and 52.4% of the children preferred green vegetables to yellow or pale vegetables. Grilled meat dishes, such as Grilled beef rib with seasoning, Bulgogi, grilled pork belly and beef steaks, were the most popular types of food eaten outside home by the children. Daily dietary fiber intake was 14.5 g in boys and 14.5 g in girls, and these intakes are low compared to the standard guidelines. The average intakes of energy and protein of the children were 84.5% and 114.0% of the Korean Recommended Dietary Allowances (RDAs), respectively. Besides energy, riboflavin, iron and calcium intakes were below the RDAs, and especially calcium intake was only 50% of the RDAs. On the other hand, thiamin, niacin, ascorbic acid, protein and phosphorus intakes exceeded the Korean RDAs. Family income or the children's body mass index (BMI) was not directly related to dietary fiber intakes. However, higher dietary fiber intakes tended to be related to higher intakes of green vegetables and fruits. Children with higher dietary fiber intake tended to prefer plant foods to animal foods. Energy and most nutrients, except heme iron and retinol, showed positive relationships with dietary fiber intake. Especially potassium and plant origin protein and calcium were highly correlated with dietary fiber intakes(r>0.6). From these results, it is concluded that dietary fiber intakes of these sixth grade elementary school children were less than the standard reference and it is anticipated to decrease further in the future with increased incomes. Therefore, increased intakes of dietary fiber by elementary school children should be promoted through nutrition education, together with the development of cooking methods and recipes utilizing green vegetables and fruits.

The Current Practices and Teacher's Perceptions of Highschool Home Economics Education -Focusing on Busan, Ulsan and Kyoungnam Area- (고등학교 가정과학의 운영실태 및 교과에 대한 담당교사들의 인식 -부산시, 울산시, 경남지역 일반계 고등학교 가정과학 담당교사를 대상으로-)

  • Kim Sang-Hee
    • Journal of Korean Home Economics Education Association
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    • v.17 no.2
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    • pp.61-77
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    • 2005
  • This study focuses on the current practices and teacher's perceptions of highschool Home Economics Education in Busan, Ulsan and Kyungnam area. Data were collected from 70 teachers with the questionnaire by mail. The results were following : 1. Home Economics have been teamed at schools of class rooms mere than 31, women's high schools and public schools. Teachers more than $70\%$ have operated and lessoned with 4 or 5 among 5 sectors of subject matters, especially emphasized family${\cdot}$human development and food${\cdot}$nutrition sectors. The most difficulties were the shortages of student's interest and reference books. 2. Teachers have cognized highly the connection of Technology${\cdot}$Home Economics and Home Economics, but evaluated lowly the job-course education among the H.E's goals. 3. Necessities of HE were evaluated highly, but student's interests by teacher's judgement lowly. Manual theses of clothing${\cdot}$textile and housing sectors have needs to alter partly. 4. Subject matters to add for the near future revision were related to dissatisfaction of H.E's matters. Teachers cognized problems about the excess, overlap, old-fashioned and irrelevant deepen levels of subject matters.

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Development of a force measurement device for curling sweeping with load cells (로드셀을 이용한 컬링 스위핑 힘 측정 장치 개발)

  • Lee, Sangcheol;Kim, Taewhan;Kil, Sekee;Choi, Sanghyup
    • Journal of the Korea Convergence Society
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    • v.8 no.11
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    • pp.49-56
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    • 2017
  • Curling sweeping is one of important motion to control the position of the curling stone, and sweeping speed and applied force to the broom pad are major research subjects. In this study, a device was developed to measure the force applied to the curling broom pad in curling sweeping motion, and two load cells were mounted between the broom pad and pad holder. Analog signals generated from the load cells were sampled about 300 times per second using a micro controller, and then converted to 10-bit digital signals. Calibration of the load cell and set up of regression equations to convert the measured electrical signals into mass (force) was done by three M1 class weights, and the developed system was designed as wearable device to minimize increasing of total weight of the broom. Same force was applied to the developed system and a force plate that was using as a reference force measurement system in field of sports, and the difference between the measured values were showed about $0.909{\pm}1.375N$(mean and standard deviation). The developed system could be applied other kinds of study which required force measurement function similar to sweeping motion.

Korean Secondary School Teachers' Professionalism in Teaching Environmental Subjects (중등교사들의 환경교육 수업에 대한 교사 전문성 분석)

  • Son, Yeon-A;Myeong, Soo-Jeong;Choi, Don-Hyung;Min, Byeong-Mee;Kim, Kyoung-Ran
    • Hwankyungkyoyuk
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    • v.22 no.2
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    • pp.43-55
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    • 2009
  • The objective of this study is to find out the professionalism of secondary school teachers in teaching environmental subjects. This research was conducted using a questionnaire survey of 431 secondary school teachers from 34 schools in Seoul and Gyeonggi Province, Korea. A 5-point Likert scale self-evaluation questionnaire on their professionalism in environmental education was developed based on Hungerford et al.'s (1994) evaluation criteria. Two middle school teachers' classes were observed, and video taping and interviewing were also conducted. The survey analysis revealed that teachers evaluated their professionalism on environmental subjects to be a little above the middle of the scale. The mean values were 2.80 for the foundation level; 2.86 for the conceptual awareness level; 2.84 for the investigation level; 2.94 for the environmental action skills level; and 2.84 for the educational application level. However, class observations revealed that there was some gap between their self-evaluation of professionalism and their actual classroom application. Both classes were not that satisfactory with regard to 'the abilities to communicate and apply ecological concepts', 'the ability to clarify the roles of different value systems for environmental issues and personal values in decision making', 'the ability to clarify their own value system regarding solutions to environmental issues', and 'the ability to apply citizen action skills for solving environmental issues and for sustainable development'. The results provide a basic reference for the improvement of environmental-related education.

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